RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Cod ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Gadus morhua
FDA Seafood List Common Name: Atlantic Cod
FDA Seafood List Market Name: Cod
Unique IEF Gel Code: 19930823gel10
Unique Web Code: ach02Srr
Tissue Aliquot Code: gadumorh 001 #4 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 1
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 1
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	51	6.3	0.0896887	4	0.666867	5.3	4.3	4.55	51.4	4.3	0.0881408
2	5.2	155	19.2	0.231446	4	1.51678	12.1	9.8	5.2	154.7	3.8	0.224598
3	5.85	257	31.8	0.237562	4	1.77855	14.1	11.5	5.85	257.4	4.2	0.238369
4	6.55	392	48.5	0.182963	6	1.85877	14.8	12	6.55	391.4	5.7	0.182773
5	6.85	424	52.4	0.0368555	4	0.272376	2.2	1.8	6.85	424	4.4	0.0349079
6	7.35	491	60.7	0.121879	5	0.993246	7.9	6.4	7.35	491.8	4.6	0.121423
7	8.15	611	75.5	0.125685	7	1.45825	11.6	9.4	8.15	611.2	6.7	0.123179
8	8.45	672	83.1	0.152948	7	1.89173	15	12.2	8.45	671.5	7	0.151877
9	8.65	726	89.7	0.119561	4	0.868791	6.9	5.6	8.65	725.1	4.1	0.118515
10	9.3	774	95.7	0.107329	7	1.27738	10.2	8.2	9.3	774.2	7.4	0.098017

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.435	29	3.6	0.0907869	6	0.900568	3.1	2.4	4.435	28.1	5.5	0.0921376
2	4.567	50	6.2	0.220196	15	5.33698	18.6	14.5	4.567	56.2	14.6	0.206682
3	5.118	138	17.1	0.0183384	5	0.1485	0.5	0.4	5.118	137.8	5.3	0.0157604
4	5.2	151	18.7	0.0717589	4	0.543632	1.9	1.5	5.2	149.9	4.1	0.0739634
5	5.289	165	20.4	0.432737	4	2.81479	9.8	7.6	5.289	165.1	3.7	0.433833
6	5.43	187	23.2	0.355155	4	1.9515	6.8	5.3	5.43	186.8	3.5	0.314302
7	5.57	209	25.9	0.0176689	16	1.2827	4.5	3.5	5.57	188.3	16.4	0.04408
8	5.711	231	28.6	0.0194016	5	0.172173	0.6	0.5	5.711	230.5	5.4	0.0180345
9	5.851	253	31.4	0.44899	4	3.05834	10.7	8.3	5.851	253.4	3.9	0.447649
10	5.95	272	33.7	0.155482	4	1.03197	3.6	2.8	5.95	271.9	3.8	0.154354
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.039	289	35.8	0.0388445	3	0.209036	0.7	0.6	6.039	288.9	3.1	0.0378722
12	6.086	298	36.9	0.129312	3	0.729976	2.5	2	6.086	297.1	3.2	0.127514
13	6.138	308	38.2	0.169326	5	1.72883	6	4.7	6.138	308.5	5.5	0.17854
14	6.237	327	40.5	0.172804	3	1.031	3.6	2.8	6.237	326.2	3.4	0.172801
15	6.32	343	42.5	0.0680743	2	0.278771	1	0.8	6.32	342.7	2.4	0.0662317
16	6.367	352	43.6	0.0378306	2	0.152591	0.5	0.4	6.367	352	2.2	0.0387085
17	6.404	359	44.5	0.0731027	3	0.395308	1.4	1.1	6.404	358.5	3.1	0.0724091
18	6.461	370	45.8	0.151536	5	1.1739	4.1	3.2	6.461	369.4	4.5	0.147038
19	6.55	387	48	0.030083	4	0.198094	0.7	0.5	6.55	387	3.6	0.0309012
20	6.85	418	51.8	0.0300359	5	0.250747	0.9	0.7	6.85	419.2	5.2	0.0274428
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.039	446	55.3	0.034456	11	0.616834	2.1	1.7	7.039	449.8	10.8	0.032126
22	7.35	492	61	0.149451	6	1.59943	5.6	4.3	7.35	492.2	6.3	0.142909
23	7.451	507	62.8	0.0526364	6	0.553401	1.9	1.5	7.451	507.2	6.1	0.0509237
24	8.15	611	75.7	0.0923574	4	0.567024	2	1.5	8.15	609.9	4.2	0.0754589
25	8.45	674	83.5	0.0721742	7	0.857597	3	2.3	8.45	673.4	6.8	0.0710715
26	8.65	725	89.8	0.0591414	4	0.378197	1.3	1	8.65	724.8	3.7	0.057424
27	9.3	774	95.9	0.0599627	8	0.741628	2.6	2	9.3	773.9	7.8	0.0534816

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.415	23	2.9	0.146598	4	0.455343	1.5	1.1	4.415	21.9	3.5	0.0732155
2	4.578	48	6	0.269147	22	9.52011	30.6	23.7	4.578	57.3	21.9	0.245058
3	5.125	132	16.4	0.0278002	2	0.12234	0.4	0.3	5.125	131.9	2.4	0.02888
4	5.197	143	17.7	0.0233951	3	0.14148	0.5	0.4	5.197	142.3	3.3	0.0240502
5	5.295	159	19.7	0.553739	3	3.07899	9.9	7.7	5.295	158.7	3.3	0.523111
6	5.436	182	22.6	0.439152	4	2.82078	9.1	7	5.436	182	4	0.394458
7	5.613	211	26.2	0.0118406	24	1.21902	3.9	3	5.613	188	24	0.0286572
8	5.717	228	28.3	0.0260621	3	0.117891	0.4	0.3	5.717	227.5	3.4	0.0194498
9	5.857	251	31.1	0.505086	4	3.14847	10.1	7.9	5.857	251.4	3.6	0.487388
10	5.947	269	33.4	0.227585	4	1.2963	4.2	3.2	5.947	269.4	3.5	0.208378
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.023	284	35.2	0.0787753	8	0.858971	2.8	2.1	6.023	279.3	7.9	0.0613238
12	6.063	292	36.2	0.143801	2	0.476788	1.5	1.2	6.063	292.2	1.9	0.144519
13	6.108	301	37.3	0.10166	5	0.923599	3	2.3	6.108	303.8	5.4	0.0962908
14	6.208	321	39.8	0.236292	4	1.33361	4.3	3.3	6.208	320.7	3.6	0.207569
15	6.294	338	41.9	0.12731	3	0.648529	2.1	1.6	6.294	337.7	2.9	0.126996
16	6.344	348	43.2	0.0351664	2	0.132417	0.4	0.3	6.344	347.5	1.9	0.0395225
17	6.374	354	43.9	0.10227	3	0.525144	1.7	1.3	6.374	354.4	3	0.097763
18	6.434	366	45.4	0.185575	5	1.56976	5	3.9	6.434	366.1	5	0.177733
19	7.029	444	55.1	0.0419191	12	0.948223	3	2.4	7.029	446.4	12.4	0.0430373
20	7.363	491	60.9	0.0493594	24	1.78115	5.7	4.4	7.363	493.1	24	0.0418719

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	40	5	0.101259	5	0.789683	6.2	5	4.55	40.6	4.6	0.0971489
2	5.2	136	16.9	0.238177	4	1.7208	13.6	11	5.2	135.4	4.2	0.233796
3	5.85	246	30.6	0.238655	5	1.89618	14.9	12.1	5.85	246	4.6	0.231426
4	6.55	391	48.6	0.208271	6	2.04428	16.1	13.1	6.55	391	5.8	0.20002
5	6.85	420	52.2	0.0464276	5	0.395556	3.1	2.5	6.85	420.5	5.1	0.0438263
6	7.35	486	60.4	0.117239	4	0.877613	6.9	5.6	7.35	486.1	4.3	0.11637
7	8.15	610	75.9	0.135487	6	1.38842	10.9	8.9	8.15	610.5	5.9	0.13321
8	8.45	672	83.6	0.156247	6	1.58213	12.5	10.1	8.45	671.5	5.7	0.157496
9	8.65	723	89.9	0.112564	4	0.786659	6.2	5	8.65	722	3.9	0.112375
10	9.3	769	95.6	0.104038	7	1.2088	9.5	7.7	9.3	769.6	7	0.0980806

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.449	28	3.5	0.0721366	6	0.763505	3	2.5	4.449	27.7	5.8	0.0740887
2	4.55	43	5.4	0.040767	1	0.0095792	0	0	4.55	43.1	0.7	0.0080014
3	4.677	62	7.7	0.199372	16	5.36131	21.4	17.6	4.677	62.6	16.4	0.184895
4	5.113	127	15.8	0.0135742	7	0.148892	0.6	0.5	5.113	129	7.4	0.0113626
5	5.2	140	17.5	0.0821828	4	0.527473	2.1	1.7	5.2	139	3.6	0.0816426
6	5.279	153	19.1	0.389383	4	2.58988	10.3	8.5	5.279	152.8	3.7	0.393737
7	5.413	175	21.8	0.291508	5	2.54176	10.1	8.4	5.413	175	5.1	0.280148
8	5.73	227	28.3	0.0166775	5	0.146529	0.6	0.5	5.73	226.2	5.4	0.0154325
9	5.867	250	31.2	0.376448	4	2.59648	10.4	8.5	5.867	250.5	3.9	0.379732
10	5.958	268	33.4	0.151407	4	1.12896	4.5	3.7	5.958	268.9	4.2	0.152398
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.039	284	35.4	0.0584661	4	0.345153	1.4	1.1	6.039	283.9	3.8	0.0512407
12	6.085	293	36.5	0.0910299	2	0.329381	1.3	1.1	6.085	292.6	2	0.0935672
13	6.13	302	37.7	0.071372	2	0.238121	1	0.8	6.13	301.2	2.2	0.0618895
14	6.15	306	38.2	0.0727983	3	0.407306	1.6	1.3	6.15	306.2	3.3	0.0699603
15	6.231	322	40.1	0.171685	3	0.850786	3.4	2.8	6.231	321.4	3.2	0.148951
16	6.317	339	42.3	0.0578054	2	0.22817	0.9	0.8	6.317	338.8	2.1	0.0601855
17	6.373	350	43.6	0.0289758	2	0.13151	0.5	0.4	6.373	348.6	2.2	0.0338387
18	6.403	356	44.4	0.0664899	4	0.420904	1.7	1.4	6.403	355.9	3.9	0.0614767
19	6.459	367	45.8	0.145654	5	1.12587	4.5	3.7	6.459	366.6	4.5	0.14037
20	6.55	385	48	0.0297673	5	0.270063	1.1	0.9	6.55	385.2	5	0.0302944
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.85	418	52.1	0.0249471	6	0.243215	1	0.8	6.85	418.8	5.9	0.0231716
22	7.115	453	56.5	0.0289078	12	0.586682	2.3	1.9	7.115	449	12.3	0.0268649
23	7.35	484	60.3	0.0873333	5	0.807741	3.2	2.7	7.35	484.7	5.1	0.0893319
24	7.447	499	62.2	0.025722	5	0.210596	0.8	0.7	7.447	499.2	4.9	0.024029
25	8.15	608	75.8	0.0846391	9	0.959692	3.8	3.2	8.15	609.4	8.6	0.0628148
26	8.45	673	83.9	0.0871516	6	0.916613	3.7	3	8.45	672.9	6.1	0.0854622
27	8.65	722	90	0.0596476	4	0.394111	1.6	1.3	8.65	721.8	4	0.0559859
28	9.3	768	95.8	0.0663789	7	0.777444	3.1	2.6	9.3	767.8	7.4	0.059501

PeakCounter: 28

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.472	28	3.5	0.121316	7	1.41039	4.7	3.8	4.472	27.1	6.9	0.114596
2	4.635	53	6.6	0.268573	13	6.0919	20.3	16.6	4.635	57.8	13.3	0.257965
3	4.929	98	12.2	0.0876083	24	3.76059	12.5	10.2	4.929	87.3	23.5	0.0902832
4	5.118	127	15.9	0.0237959	1	0.0068903	0	0	5.118	127.5	0.5	0.0065045
5	5.197	139	17.4	0.023661	2	0.0564621	0.2	0.2	5.197	139	1.9	0.0164573
6	5.294	155	19.4	0.549755	3	2.88599	9.6	7.8	5.294	154.8	3.1	0.52297
7	5.44	179	22.3	0.419172	4	2.30465	7.7	6.3	5.44	178.5	3.5	0.367125
8	5.616	208	26	0.0243198	24	1.85882	6.2	5	5.616	181.8	24	0.0436978
9	5.731	227	28.3	0.0226827	3	0.0938504	0.3	0.3	5.731	227.4	3.3	0.0158409
10	5.873	251	31.3	0.492001	3	2.86897	9.5	7.8	5.873	251.1	3.4	0.481468
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.955	267	33.3	0.202831	4	1.33743	4.4	3.6	5.955	267.8	3.7	0.201834
12	6.026	281	35.1	0.0711361	2	0.304548	1	0.8	6.026	281.4	2.5	0.0694104
13	6.067	289	36.1	0.140589	2	0.528037	1.8	1.4	6.067	289.2	2.3	0.13047
14	6.108	297	37.1	0.112926	6	1.06904	3.6	2.9	6.108	298.5	5.8	0.103397
15	6.21	317	39.6	0.206228	3	1.14831	3.8	3.1	6.21	316.5	3.5	0.186452
16	6.297	334	41.7	0.0846905	2	0.342096	1.1	0.9	6.297	333.9	2.3	0.0840791
17	6.353	345	43.1	0.0388998	3	0.189144	0.6	0.5	6.353	344.1	2.6	0.0411305
18	6.384	351	43.8	0.0911019	3	0.506799	1.7	1.4	6.384	351.2	3.4	0.0841005
19	6.445	363	45.3	0.180215	6	1.64768	5.5	4.5	6.445	363	5.5	0.167999
20	7.109	450	56.2	0.0412453	12	0.786132	2.6	2.1	7.109	447	11.7	0.0379879
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.385	488	60.9	0.0492346	11	0.869724	2.9	2.4	7.385	488.1	11.1	0.0441627

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	37	4.6	0.097827	4	0.74575	6.2	4.9	4.55	37.5	4.4	0.0955981
2	5.2	139	17.4	0.224885	4	1.54948	12.8	10.2	5.2	138.5	3.9	0.224239
3	5.85	246	30.7	0.253825	4	1.85255	15.3	12.2	5.85	245.9	4.2	0.248461
4	6.55	382	47.7	0.191356	6	1.79555	14.8	11.8	6.55	381.7	5.7	0.179155
5	6.85	412	51.4	0.0373269	5	0.30164	2.5	2	6.85	413.2	5.1	0.0335781
6	7.35	481	60	0.113847	4	0.833312	6.9	5.5	7.35	481.1	4.1	0.11543
7	8.15	608	75.9	0.131152	6	1.46833	12.1	9.7	8.15	607.2	6.3	0.132441
8	8.45	674	84.1	0.159201	5	1.53268	12.7	10.1	8.45	674	5.5	0.158557
9	8.65	724	90.4	0.11525	4	0.851108	7	5.6	8.65	723.6	4.3	0.112035
10	9.3	769	96	0.123071	6	1.16471	9.6	7.7	9.3	769.1	5.6	0.118073

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
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PeakCount: 126

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 5.300 5.200 12.100 5.850 14.100 6.550 14.800 6.850 2.200 7.350 7.900 8.150 11.600 8.450 15.000 8.650 6.900 9.300 10.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 1 LANE TYPE: pI Markers ACTUAL GEL LANE: 1 PeakCount: 10 Return to Lane Navigator
4.435 3.100 4.567 18.600 5.118 0.500 5.200 1.900 5.289 9.800 5.430 6.800 5.570 4.500 5.711 0.600 5.851 10.700 5.950 3.600 6.039 0.700 6.086 2.500 6.138 6.000 6.237 3.600 6.320 1.000 6.367 0.500 6.404 1.400 6.461 4.100 6.550 0.700 6.850 0.900 7.039 2.100 7.350 5.600 7.451 1.900 8.150 2.000 8.450 3.000 8.650 1.300 9.300 2.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 2 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 2 PeakCount: 27 Return to Lane Navigator
4.415 1.500 4.578 30.600 5.125 0.400 5.197 0.500 5.295 9.900 5.436 9.100 5.613 3.900 5.717 0.400 5.857 10.100 5.947 4.200 6.023 2.800 6.063 1.500 6.108 3.000 6.208 4.300 6.294 2.100 6.344 0.400 6.374 1.700 6.434 5.000 7.029 3.000 7.363 5.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 20 Return to Lane Navigator
4.550 6.200 5.200 13.600 5.850 14.900 6.550 16.100 6.850 3.100 7.350 6.900 8.150 10.900 8.450 12.500 8.650 6.200 9.300 9.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.449 3.000 4.550 0 4.677 21.400 5.113 0.600 5.200 2.100 5.279 10.300 5.413 10.100 5.730 0.600 5.867 10.400 5.958 4.500 6.039 1.400 6.085 1.300 6.130 1.000 6.150 1.600 6.231 3.400 6.317 0.900 6.373 0.500 6.403 1.700 6.459 4.500 6.550 1.100 6.850 1.000 7.115 2.300 7.350 3.200 7.447 0.800 8.150 3.800 8.450 3.700 8.650 1.600 9.300 3.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 28 Return to Lane Navigator
4.472 4.700 4.635 20.300 4.929 12.500 5.118 0 5.197 0.200 5.294 9.600 5.440 7.700 5.616 6.200 5.731 0.300 5.873 9.500 5.955 4.400 6.026 1.000 6.067 1.800 6.108 3.600 6.210 3.800 6.297 1.100 6.353 0.600 6.384 1.700 6.445 5.500 7.109 2.600 7.385 2.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 21 Return to Lane Navigator
4.550 6.200 5.200 12.800 5.850 15.300 6.550 14.800 6.850 2.500 7.350 6.900 8.150 12.100 8.450 12.700 8.650 7.000 9.300 9.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Atlantic Cod ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Cod ---- Go to Display Gel Lane Navigator.