A STUDY OF BAT HABITAT AND ACTIVITY AT TWO SITES IN DUBUQUE COUNTY, IOWA: MINES OF SPAIN AND WHITE PINE HOLLOW Brian Lex and Laura Cady. Environmental Science Program, Biology Department University of Dubuque, Dubuque, IA 52001 A study of bat activity at two sites in eastern Dubuque County, IA was conducted to determine species diversity, habitat suitability and effects due to human disturbances. Mist netting for this experiment compared habitats at Mines of Spain along Catfish creek located near the Mississippi River, and along the Little Turkey River in White Pine Hollow. Human disturbance, temperature, and rainfall effects were evaluated as they may have influenced bat inhabitation of the sites and activity levels. Greater species diversity was observed at White Pine Hollow, where four different species were captured. These included the little brown bat (Myotis lucifugus), big brown bat (Eptesicus fuscus), silver- haired bat (Lasionycteris noctivangans), and the endangered Indiana bat (Myotis sodalis). Only the big brown bat was captured at the Mines of Spain site. White Pine Hollow is a preserve with few human disturbances, and Mines of Spain is a recreational area visited frequently by humans. Greater numbers of bats were observed following the wet spring than in the drier summer months. Keywords: Chiropterans, bats, White Pine Hollow, Mines of Spain