Conley AH, Kessler JA, Boots LJ, Lineberger D, Schleif WA, Emini EA; National Conference on Human Retroviruses and Related Infections.
Program Abstr First Natl Conf Hum Retrovir Relat Infect Natl Conf Hum Retrovir Relat Infect 1st 1993 Wash DC. 1993 Dec 12-16; 67.
Merck Research Laboratories, West Point, PA.
The third variable region (V3) of the HIV-1 gp120 can induce potent, virus neutralizing antibodies. We recently reported the isolation and characterization of an anti-V3 human MAb, 447-52D that neutralized all HIV- 1 laboratory strains that express the antibody's binding determinant GPGR, in the apex of the V3 loop. We now show that 447-52D efficiently neutralizes primary HIV-1 isolates in human PBMC cultures using an infectivity reduction assay. Neutralization is dependent on the presence of GPGR in the isolate's V3 sequence. Some isolates are completely neutralized by as little as 10 micrograms/mL MAb, when viral input doses are 50-500 pg p24. 100% neutralization of infectivity can be observed for some isolates where virus inputs as high as 1000 pg p24 are used. This antibody also can neutralize infectious HIV-1 directly from patient plasma ex vivo. The data support an immunoprophylactic role for such broadly reactive virus-neutralizing anti-V3 antibodies.
Publication Types:
Keywords:
- Animals
- Antibodies, Monoclonal
- Base Sequence
- HIV Envelope Protein gp120
- HIV-1
- Humans
- genetics
- immunology
Other ID:
UI: 102214038
From Meeting Abstracts