RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Pollock ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Pollachius virens
FDA Seafood List Common Name: Pollock
FDA Seafood List Market Name: Pollock
Unique IEF Gel Code: 19940912gel09
Unique Web Code: aph01Srr
Tissue Aliquot Code: pollvire H94-002 #04a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	28	3.6	0.0723167	5	0.645112	10	8.2	4.55	28.4	5.3	0.0687704
2	5.2	129	16.6	0.0898885	4	0.664316	10.2	8.5	5.2	129	4.3	0.0881574
3	5.85	233	30.1	0.102699	4	0.803352	12.4	10.2	5.85	232.8	4.5	0.101344
4	6.55	364	47	0.0563445	9	0.871378	13.4	11.1	6.55	362.3	8.8	0.0557977
5	6.85	395	51	0.0142834	5	0.12056	1.9	1.5	6.85	395	5.1	0.0132472
6	7.35	462	59.6	0.068995	5	0.58586	9	7.5	7.35	462.4	4.9	0.0679068
7	8.15	582	75.1	0.0515781	7	0.620339	9.6	7.9	8.15	582.5	7.1	0.0494457
8	8.45	644	83.1	0.0760592	6	0.856136	13.2	10.9	8.45	643.1	6.4	0.0752818
9	8.65	695	89.7	0.0476325	5	0.426559	6.6	5.4	8.65	694.6	5	0.04858
10	9.3	746	96.3	0.0586275	9	0.888219	13.7	11.3	9.3	744.6	9.4	0.0534835

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.612	38	4.9	0.282295	8	2.9821	8.9	6.2	4.612	36.2	8.5	0.198382
2	4.749	58	7.5	0.232781	20	8.29219	24.9	17.2	4.749	56	20.4	0.22985
3	5.038	100	12.9	0.141443	14	3.14128	9.4	6.5	5.038	101.2	13.6	0.129931
4	5.251	132	17	0.0177028	2	0.0325723	0.1	0.1	5.251	132.8	2	0.0092133
5	5.354	149	19.2	0.57738	5	4.3964	13.2	9.1	5.354	149	4.5	0.550328
6	5.469	168	21.7	0.09924	14	2.43087	7.3	5	5.469	164.2	13.7	0.100349
7	5.711	208	26.8	0.0145436	8	0.175437	0.5	0.4	5.711	210.4	7.5	0.0131826
8	5.97	254	32.8	0.031881	8	0.380676	1.1	0.8	5.97	252.1	7.8	0.0276848
9	6.069	273	35.2	0.418551	4	3.04807	9.1	6.3	6.069	272.7	4.2	0.40712
10	6.143	287	37	0.336491	5	2.98004	8.9	6.2	6.143	286.7	5.4	0.313626
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.216	301	38.8	0.210805	3	0.96443	2.9	2	6.216	300.5	2.6	0.206045
12	6.268	311	40.1	0.0319936	3	0.180717	0.5	0.4	6.268	312.3	2.7	0.0370854
13	6.31	319	41.2	0.0662805	4	0.401482	1.2	0.8	6.31	319.5	3.5	0.0641584
14	6.378	332	42.8	0.0637355	4	0.447651	1.3	0.9	6.378	331.2	4	0.0632125
15	6.466	349	45	0.154129	4	1.1477	3.4	2.4	6.466	347.8	4.5	0.145308
16	6.662	376	48.5	0.0130992	7	0.207693	0.6	0.4	6.662	375.3	7.2	0.0163452
17	9.109	731	94.3	0.0791398	16	2.11313	6.3	4.4	9.109	729.6	16.1	0.0740233

PeakCounter: 17

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	32	4.1	0.0668888	5	0.590283	9.5	7.5	4.55	31.7	5.1	0.0649236
2	5.2	120	15.4	0.0829157	4	0.624523	10	8	5.2	120.1	4.4	0.0800167
3	5.85	231	29.7	0.111658	4	0.694163	11.1	8.8	5.85	230.7	3.5	0.111409
4	6.55	368	47.4	0.0607959	8	0.873171	14	11.1	6.55	365.7	8.2	0.0599588
5	6.85	396	51	0.0156078	7	0.152563	2.4	1.9	6.85	397.3	6.5	0.013201
6	7.35	462	59.5	0.0636968	5	0.53583	8.6	6.8	7.35	461.8	4.9	0.0617236
7	8.15	585	75.3	0.0590667	7	0.770311	12.4	9.8	8.15	585.5	7.4	0.0583579
8	8.45	646	83.1	0.0685689	7	0.808543	13	10.3	8.45	646.5	6.7	0.0682014
9	8.65	697	89.7	0.042565	5	0.347613	5.6	4.4	8.65	696.2	4.8	0.0410093
10	9.3	748	96.3	0.0544854	10	0.832417	13.4	10.6	9.3	747	9.8	0.0479952

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	38	4.9	0.0079052	2	0.0285322	0.1	0.1	4.55	37.1	1.8	0.0087459
2	4.663	55	7.1	0.092277	7	0.496466	2.5	2	4.663	50.8	6.6	0.0424734
3	4.749	68	8.8	0.146274	14	3.31099	16.8	13.2	4.749	67.4	13.9	0.13412
4	4.981	103	13.3	0.0533344	17	1.6655	8.4	6.6	4.981	97.6	17.2	0.0547814
5	5.2	136	17.5	0.0371929	3	0.201189	1	0.8	5.2	136.2	3.4	0.0337913
6	5.3	152	19.6	0.42727	4	2.59639	13.1	10.3	5.3	152.1	3.6	0.408114
7	5.412	170	21.9	0.0621946	11	1.10229	5.6	4.4	5.412	165.3	11.3	0.0549987
8	5.625	204	26.3	0.0294198	7	0.350348	1.8	1.4	5.625	205.5	7.2	0.0275139
9	5.737	222	28.6	0.016482	4	0.119898	0.6	0.5	5.737	221.8	4.3	0.0157505
10	5.85	240	30.9	0.0633371	6	0.591461	3	2.4	5.85	240.7	5.6	0.0600912
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.991	266	34.2	0.41031	4	2.62123	13.3	10.4	5.991	266.6	3.6	0.405347
12	6.083	283	36.4	0.211985	6	1.99137	10.1	7.9	6.083	282.6	5.7	0.198297
13	6.165	298	38.4	0.101694	3	0.54406	2.8	2.2	6.165	297.9	3.3	0.0941757
14	6.268	317	40.8	0.0171577	2	0.0699752	0.4	0.3	6.268	317.2	2.1	0.0185783
15	6.349	332	42.7	0.0168504	3	0.0789691	0.4	0.3	6.349	332.4	2.7	0.0162846
16	6.447	350	45	0.0954625	7	0.99883	5.1	4	6.447	348.3	6.6	0.0848073
17	6.517	363	46.7	0.0125929	4	0.113757	0.6	0.5	6.517	361.7	4.4	0.0145272
18	7.35	466	60	0.0256024	6	0.276011	1.4	1.1	7.35	464	5.8	0.0267358
19	8.15	590	75.9	0.0177845	24	0.919619	4.7	3.7	8.15	575.9	23.9	0.021748
20	8.45	652	83.9	0.0381099	8	0.493042	2.5	2	8.45	651.3	7.6	0.0366023
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.65	700	90.1	0.0243894	5	0.204464	1	0.8	8.65	699.2	5	0.0230645
22	9.3	750	96.5	0.0342394	16	0.986367	5	3.9	9.3	742.1	16.5	0.0338244

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.58	42	5.4	0.245793	8	3.05133	11.7	8.1	4.58	39.5	8	0.215556
2	4.668	55	7.1	0.178926	10	2.81712	10.8	7.5	4.668	52.7	10.1	0.157747
3	4.782	72	9.3	0.124033	13	2.74845	10.5	7.3	4.782	71	12.8	0.121432
4	5.052	112	14.4	0.0195152	4	0.120184	0.5	0.3	5.052	111.2	3.8	0.0177309
5	5.212	136	17.5	0.0111031	3	0.0644493	0.2	0.2	5.212	133.5	3.4	0.0107431
6	5.293	149	19.2	0.629255	4	3.88394	14.8	10.3	5.293	149.4	3.6	0.608218
7	5.405	167	21.5	0.132693	11	2.1468	8.2	5.7	5.405	163.5	11	0.109899
8	5.579	195	25.1	0.0163815	3	0.0797264	0.3	0.2	5.579	195.7	2.8	0.0158132
9	5.667	209	26.9	0.0098017	3	0.0481949	0.2	0.1	5.667	208.9	3	0.0090883
10	5.869	242	31.1	0.0219896	9	0.35119	1.3	0.9	5.869	243.4	9.2	0.0214959
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.996	266	34.2	0.600074	4	3.72841	14.2	9.9	5.996	266.2	3.5	0.593301
12	6.087	283	36.4	0.303337	6	3.07667	11.8	8.2	6.087	282.7	6.2	0.279232
13	6.183	301	38.7	0.143323	3	0.750171	2.9	2	6.183	300.5	3	0.141607
14	6.241	312	40.2	0.0239551	3	0.142176	0.5	0.4	6.241	312.8	3.3	0.0243575
15	6.289	321	41.3	0.0446151	3	0.255622	1	0.7	6.289	320.8	3.2	0.045332
16	6.358	334	43	0.017826	2	0.0853152	0.3	0.2	6.358	334.7	2.5	0.0193665
17	6.449	351	45.2	0.167178	6	1.58478	6.1	4.2	6.449	350.4	5.9	0.150809
18	6.572	372	47.9	0.0166732	7	0.206908	0.8	0.5	6.572	375.7	7.3	0.0159033
19	9.153	741	95.4	0.0620552	10	1.02624	3.9	2.7	9.153	738.8	10.3	0.0560014

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	37	4.8	0.0665056	6	0.636054	10.2	8.1	4.55	37	5.9	0.0610333
2	5.2	132	17	0.0773559	4	0.57616	9.3	7.3	5.2	132.2	4.3	0.0753335
3	5.85	237	30.5	0.0991493	5	0.836416	13.5	10.7	5.85	237.4	4.9	0.0968096
4	6.55	371	47.7	0.0519757	8	0.688009	11.1	8.8	6.55	369.8	7.6	0.0512462
5	6.85	398	51.2	0.0119052	6	0.113826	1.8	1.5	6.85	399	5.6	0.0114886
6	7.35	467	60.1	0.0382847	5	0.333818	5.4	4.3	7.35	467.3	5.2	0.0363166
7	8.15	588	75.7	0.0592211	7	0.711768	11.5	9.1	8.15	586.5	7.1	0.0568926
8	8.45	651	83.8	0.0605104	8	0.848288	13.6	10.8	8.45	650.8	8.1	0.0592062
9	8.65	703	90.5	0.0407734	6	0.383346	6.2	4.9	8.65	701.8	5.5	0.0392032
10	9.3	755	97.2	0.0859822	7	1.08736	17.5	13.9	9.3	753.7	7.2	0.0848133

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 88

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 10.000 5.200 10.200 5.850 12.400 6.550 13.400 6.850 1.900 7.350 9.000 8.150 9.600 8.450 13.200 8.650 6.600 9.300 13.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.612 8.900 4.749 24.900 5.038 9.400 5.251 0.100 5.354 13.200 5.469 7.300 5.711 0.500 5.970 1.100 6.069 9.100 6.143 8.900 6.216 2.900 6.268 0.500 6.310 1.200 6.378 1.300 6.466 3.400 6.662 0.600 9.109 6.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 17 Return to Lane Navigator
4.550 9.500 5.200 10.000 5.850 11.100 6.550 14.000 6.850 2.400 7.350 8.600 8.150 12.400 8.450 13.000 8.650 5.600 9.300 13.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.550 0.100 4.663 2.500 4.749 16.800 4.981 8.400 5.200 1.000 5.300 13.100 5.412 5.600 5.625 1.800 5.737 0.600 5.850 3.000 5.991 13.300 6.083 10.100 6.165 2.800 6.268 0.400 6.349 0.400 6.447 5.100 6.517 0.600 7.350 1.400 8.150 4.700 8.450 2.500 8.650 1.000 9.300 5.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 22 Return to Lane Navigator
4.580 11.700 4.668 10.800 4.782 10.500 5.052 0.500 5.212 0.200 5.293 14.800 5.405 8.200 5.579 0.300 5.667 0.200 5.869 1.300 5.996 14.200 6.087 11.800 6.183 2.900 6.241 0.500 6.289 1.000 6.358 0.300 6.449 6.100 6.572 0.800 9.153 3.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 19 Return to Lane Navigator
4.550 10.200 5.200 9.300 5.850 13.500 6.550 11.100 6.850 1.800 7.350 5.400 8.150 11.500 8.450 13.600 8.650 6.200 9.300 17.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Pollock ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Pollock ---- Go to Display Gel Lane Navigator.