| J Clin Microbiol. 1997 June; 35(6): 1404–1410. | PMCID: PMC229758 |
Analysis of incidence of infection with enterotoxigenic Escherichia coli in a prospective cohort study of infant diarrhea in Nicaragua. M Paniagua, F Espinoza, M Ringman, E Reizenstein, A M Svennerholm, and H Hallander Department of Microbiology, National Autonomous University (UNAN), Léon, Nicaragua. Abstract Diarrheal episodes with enterotoxigenic Escherichia coli (ETEC) were prospectively monitored during the first 2 years of life in a cohort of 235 infants from Leon, Nicaragua. ETEC was an etiological finding in 38% (310 of 808) of diarrheal episodes and in 19% (277 of 1,472) of samples taken as asymptomatic controls at defined age intervals (P = <0.0001). The majority of diarrheal episodes (80%) occurred before 12 months of age. The major ETEC type was characterized by colonization factor CFA I and elaboration of both heat-labile enterotoxin and heat-stable enterotoxin (ST). The proportion of E. coli strains with CFA I was significantly higher in cases with diarrhea (P = 0.002). The second most prevalent type showed putative colonization factor PCFO166 and production of ST. The prevalence of PCFO166 was approximately 20%, higher than reported before. Children with a first CFA I episode contracted a second ETEC CFA I infection 24% of the time, compared with 46% for ETEC strains of any subtype. Most of the ETEC episodes were of moderate severity, and only 5% (15 of 310) were characterized as severe. In conclusion, our results give valuable information for the planning of intervention studies using ETEC vaccines. Full Text The Full Text of this article is available as a PDF (181K). These references are in PubMed. This may not be the complete list of references from this article. - Abe, A; Obata, H; Matsushita, S; Yamada, S; Kudoh, Y; Bangtrakulnonth, A; Ratchtrachenchat, OA; Danbara, H. A sensitive method for the detection of enterotoxigenic Escherichia coli by the polymerase chain reaction using multiple primer pairs. Zentralbl Bakteriol. 1992 Jul;277(2):170–178. [PubMed]
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