MT0126

Library Description

Organism:	Homo sapiens
Developmental stage:	Adult
Organ:	marrow
Vector:	puc18
Vector type:	Plasmid
Cloning site 1:	SmaI
Cloning site 2:	SmaI

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

417 ESTs from this library were grouped into 109 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
94	225420	Hs.78466	Proteasome (prosome, macropain) 26S subunit, non-ATPase, 8.
37	88729	Hs.406683	Ribosomal protein S15.
31	74341	Hs.714226	Transcribed locus, strongly similar to NP_006808.1 endoplasmic reticulum protein 29 isoform 1 precursor [Homo sapiens].
13	31175	Hs.520048	Major histocompatibility complex, class II, DR alpha.
13	31175	Hs.4859	Cyclin L1.
11	26379	Hs.75841	Endoplasmic reticulum protein 29.
11	26379	Hs.708545	Transcribed locus.
9	21583	Hs.712144	SID1 transmembrane family, member 2.
7	16787	Hs.711183	Transcribed locus, moderately similar to NP_066362.2 interferon-induced transmembrane protein 3 (1-8U) [Homo sapiens].
7	16787	Hs.591582	Vacuolar protein sorting 24 homolog (S. cerevisiae).
7	16787	Hs.108408	Anterior pharynx defective 1 homolog A (C. elegans).
6	14388	Hs.103183	Fragile X mental retardation 1.
5	11990	Hs.639520	Similar to hCG37602.
5	11990	Hs.631886	V-rel reticuloendotheliosis viral oncogene homolog (avian).
5	11990	Hs.426104	Transcribed locus, strongly similar to NP_058847.1 ribosomal protein S15 [Rattus norvegicus].
5	11990	Hs.408073	Ribosomal protein S6.
5	11990	Hs.314359	Eukaryotic translation initiation factor 3, subunit K.
5	11990	Hs.170999	Lupus brain antigen 1.
4	9592	Hs.658285	CDNA FLJ36442 fis, clone THYMU2012631.
4	9592	Hs.488478	Chromosome 7 open reading frame 42.
4	9592	Hs.471014	Talin 1.
4	9592	Hs.2533	Aldehyde dehydrogenase 9 family, member A1.
3	7194	Hs.713310	Transcribed locus.
3	7194	Hs.656274	Tumor necrosis factor, alpha-induced protein 8.
3	7194	Hs.642877	Metastasis associated lung adenocarcinoma transcript 1 (non-protein coding).
3	7194	Hs.407771	Cancer susceptibility candidate 1.
3	7194	Hs.211571	Holocytochrome c synthase (cytochrome c heme-lyase).
3	7194	Hs.168950	Ganglioside-induced differentiation-associated protein 1.
3	7194	Hs.150580	Eukaryotic translation initiation factor 1.
2	4796	Hs.714771	Kynurenine 3-monooxygenase (kynurenine 3-hydroxylase).
2	4796	Hs.712749	Transcribed locus.
2	4796	Hs.711490	Programmed cell death 4 (neoplastic transformation inhibitor).
2	4796	Hs.708239	Breast carcinoma amplified sequence 4.
2	4796	Hs.680325	Transcribed locus.
2	4796	Hs.654514	Protein tyrosine phosphatase, receptor type, C.
2	4796	Hs.632554	Splicing factor 3b, subunit 1, 155kDa.
2	4796	Hs.580351	Protein kinase C, epsilon.
2	4796	Hs.529303	Actin related protein 2/3 complex, subunit 2, 34kDa.
2	4796	Hs.513851	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide.
2	4796	Hs.503043	Carnitine palmitoyltransferase 1A (liver).
2	4796	Hs.500409	Glutamate dehydrogenase 1.
2	4796	Hs.491351	Clathrin, heavy chain (Hc).
2	4796	Hs.486031	Activating signal cointegrator 1 complex subunit 3.
2	4796	Hs.475392	Transmembrane protein 111.
2	4796	Hs.44811	Transcribed locus.
2	4796	Hs.432491	Esterase D/formylglutathione hydrolase.
2	4796	Hs.431498	Forkhead box P1.
2	4796	Hs.425125	Ribosomal protein L29.
2	4796	Hs.388903	Transcribed locus.
2	4796	Hs.3260	Presenilin 1.
2	4796	Hs.284630	TBC1 domain family, member 15.
2	4796	Hs.191346	Septin 7.
2	4796	Hs.105379	LSM14B, SCD6 homolog B (S. cerevisiae).
1	2398	Hs.93842	StAR-related lipid transfer (START) domain containing 4.
1	2398	Hs.93659	Protein disulfide isomerase family A, member 4.
1	2398	Hs.81964	SEC24 family, member C (S. cerevisiae).
1	2398	Hs.8102	Ribosomal protein S20.
1	2398	Hs.714443	Transcribed locus, strongly similar to NP_989488.2 eukaryotic translation elongation factor 1 alpha 1 [Gallus gallus].
1	2398	Hs.713945	Transcribed locus, strongly similar to NP_031866.2 DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 [Mus musculus].
1	2398	Hs.712994	Transcribed locus.
1	2398	Hs.712948	CDNA FLJ36515 fis, clone TRACH2001810.
1	2398	Hs.707624	Transcribed locus, strongly similar to NP_001242.2 CD68 antigen isoform A [Homo sapiens].
1	2398	Hs.706927	Transcribed locus.
1	2398	Hs.701265	Transcribed locus.
1	2398	Hs.700696	Insulin-like growth factor 2 mRNA binding protein 3.
1	2398	Hs.685604	Transcribed locus.
1	2398	Hs.682879	Transcribed locus.
1	2398	Hs.675108	Transcribed locus.
1	2398	Hs.670653	Transcribed locus.
1	2398	Hs.669238	Transcribed locus.
1	2398	Hs.667191	Transcribed locus.
1	2398	Hs.664952	SLC7A5 pseudogene.
1	2398	Hs.654597	ArfGAP with coiled-coil, ankyrin repeat and PH domains 2.
1	2398	Hs.654594	Ras homolog gene family, member H.
1	2398	Hs.643181	Hypothetical protein LOC100134713.
1	2398	Hs.637874	Transcribed locus.
1	2398	Hs.632340	Transcribed locus.
1	2398	Hs.626836	Transcribed locus.
1	2398	Hs.626835	Transcribed locus.
1	2398	Hs.61812	Protein tyrosine phosphatase, non-receptor type 12.
1	2398	Hs.601001	Transcribed locus, weakly similar to XP_518384.2 PREDICTED: similar to HLA class II histocompatibility antigen, DR alpha chain precursor (MHC class II antigen DRA) [Pan troglodytes].
1	2398	Hs.587802	Transcribed locus.
1	2398	Hs.586423	Eukaryotic translation elongation factor 1 alpha 1.
1	2398	Hs.564847	Small nuclear ribonucleoprotein polypeptide N.
1	2398	Hs.533514	Acetylserotonin O-methyltransferase-like.
1	2398	Hs.529989	Ribonuclease T2.
1	2398	Hs.524195	Rho GTPase activating protein 21.
1	2398	Hs.518203	Poly (ADP-ribose) polymerase family, member 14.
1	2398	Hs.513000	CDNA clone IMAGE:5299888.
1	2398	Hs.506603	Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2.
1	2398	Hs.495656	Transducin (beta)-like 1X-linked.
1	2398	Hs.483494	Eukaryotic translation termination factor 1.
1	2398	Hs.482017	Leucine rich repeat containing 8 family, member B.
1	2398	Hs.475319	Leucine rich repeat (in FLII) interacting protein 2.
1	2398	Hs.472508	Peptidylprolyl isomerase E pseudogene.
1	2398	Hs.443121	Purine-rich element binding protein A.
1	2398	Hs.439200	KIAA0090.
1	2398	Hs.389461	Unc-45 homolog A (C. elegans).
1	2398	Hs.368538	Glutamate dehydrogenase 2.
1	2398	Hs.318547	Protein tyrosine phosphatase, receptor type, J.