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Title Endothelial diaphragmed fenestrae: in vitro modulation by phorbol myristate acetate
Creator/Author Lombardi, T. ; Montesano, R. ; Furie, M.B. ; Silverstein, S.C. ; Orci, L.
Publication Date1986 Jan 01
OSTI IdentifierOSTI ID: 5786695
Other Number(s)CODEN: JCLBA
Resource TypeJournal Article
Resource RelationJ. Cell Biol. ; Vol/Issue: 102
Research OrgUniv. of Geneva Medical School, Switzerland
Subject550200 -- Biochemistry; ENDOTHELIUM-- CELL DIFFERENTIATION; CELL CULTURES;SCANNING ELECTRON MICROSCOPY;TUMOR PROMOTERS
Related SubjectANIMAL TISSUES;BODY;ELECTRON MICROSCOPY;MICROSCOPY;PROMOTERS;TISSUES
Description/Abstract Cultured microvascular endothelial cells isolated from fenestrated capillaries have been shown to express many properties of their in vivo differentiated phenotype, yet they contain very few diaphragmed fenestrae.^We show here that treatment of capillary endothelial cells with the tumor promoter, 4..beta..-phorbol, 12-myristate, 13-acetate, induces more than a fivefold increase in the frequency of fenestrae per ..mu..m/sup 2/ of cell surface, as determined from a quantitative evaluation on freeze-fracture replicas.^In quick-frozen, deep-etched preparations, the endothelial fenestrae appeared to be bridged by a diaphragm composed of radial fibers interweaving in a central mesh, as previously observed in vivo.^These results indicate that diaphragmed fenestrae are inducible structures, and provide an opportunity to study them in vitro.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 1965-1970
System Entry Date2001 May 13

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