Bibliographic Citation
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Title | Endothelial diaphragmed fenestrae: in vitro modulation by phorbol myristate acetate |
Creator/Author | Lombardi, T. ; Montesano, R. ; Furie, M.B. ; Silverstein, S.C. ; Orci, L. |
Publication Date | 1986 Jan 01 |
OSTI Identifier | OSTI ID: 5786695 |
Other Number(s) | CODEN: JCLBA |
Resource Type | Journal Article |
Resource Relation | J. Cell Biol. ; Vol/Issue: 102 |
Research Org | Univ. of Geneva Medical School, Switzerland |
Subject | 550200 -- Biochemistry; ENDOTHELIUM-- CELL DIFFERENTIATION; CELL CULTURES;SCANNING ELECTRON MICROSCOPY;TUMOR PROMOTERS |
Related Subject | ANIMAL TISSUES;BODY;ELECTRON MICROSCOPY;MICROSCOPY;PROMOTERS;TISSUES |
Description/Abstract | Cultured microvascular endothelial cells isolated from fenestrated capillaries have been shown to express many properties of their in vivo differentiated phenotype, yet they contain very few diaphragmed fenestrae.^We show here that treatment of capillary endothelial cells with the tumor promoter, 4..beta..-phorbol, 12-myristate, 13-acetate, induces more than a fivefold increase in the frequency of fenestrae per ..mu..m/sup 2/ of cell surface, as determined from a quantitative evaluation on freeze-fracture replicas.^In quick-frozen, deep-etched preparations, the endothelial fenestrae appeared to be bridged by a diaphragm composed of radial fibers interweaving in a central mesh, as previously observed in vivo.^These results indicate that diaphragmed fenestrae are inducible structures, and provide an opportunity to study them in vitro. |
Country of Publication | United States |
Language | English |
Format | Pages: 1965-1970 |
System Entry Date | 2001 May 13 |
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