De Jong JJ, Tersmette M, Krone W, Meloen R, Miedema F, Goudsmit J; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 601 (abstract no. W.C.P.56).
Human Retrovirus Laboratory, Amsterdam, the Netherlands
OBJECTIVE: To study the antigenic variability of the V3 region (aa 296-331) of the external envelope of 120 kDa (gp120) of HIV-1 in natural infection. METHODS: Sequential isolates of two patients progressing to AIDS and showing a transition from non-syncytium to syncytium forming capacity were used to study antigenic variability in the V3 domain of gp120. The V3 coding domain was amplified using the polymerase chain reaction (PCR). Direct sequences of the V3 coding domain were determined and confirmed by cloning and sequencing. Antibody recognition of sequential sera of these individuals was determined for peptides covering the complete V3 domain of the isolates. RESULTS: The transition from non-syncytium to syncytium inducing isolates was marked by clustered nucleic acid changes of which three were consistent. This resulted in one amino acid changes crucial to antibody binding. Decline of the antibody to the syncytium forming antigenic variants preceded the emergence of these variants. CONCLUSION: Antigenic variants appear to be selected by antibody to the V3 domain of gp120 during natural infection.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Antibodies
- Antigenic Variation
- Base Sequence
- Binding Sites, Antibody
- Giant Cells
- HIV Antibodies
- HIV Envelope Protein gp120
- HIV-1
- Humans
- Polymerase Chain Reaction
- genetics
- immunology
Other ID:
UI: 102179065
From Meeting Abstracts