NCI_CGAP_GC3

Library Description

Organism:	Homo sapiens
Tissue:	pooled germ cell tumors
Vector:	pT7T3D-PacI
Vector type:	phagemid (ampicillin resistant)
Lab host:	DH10B

1st strand cDNA was prepared from 3 pooled germ cell tumors, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is not normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo.

Source: Robert Strausberg, Ph.D.

Gene Content Analysis

2,506 ESTs from this library were grouped into 1,506 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
71	28332	Hs.510635	Immunoglobulin heavy constant gamma 1 (G1m marker).
59	23543	Hs.433670	Ferritin, light polypeptide.
58	23144	Hs.449585	Immunoglobulin lambda locus.
38	15164	Hs.449621	Immunoglobulin kappa locus.
20	7981	Hs.438429	Ribosomal protein S19.
13	5188	Hs.522584	Thymosin beta 4, X-linked.
12	4789	Hs.544577	Glyceraldehyde-3-phosphate dehydrogenase.
12	4789	Hs.469473	Ribosomal protein L31.
12	4789	Hs.433701	Ribosomal protein L37a.
12	4789	Hs.275865	Similar to ribosomal protein S18.
11	4389	Hs.8102	Ribosomal protein S20.
11	4389	Hs.280130	Ribosomal protein S24.
10	3990	Hs.449909	Ribosomal protein SA.
9	3591	Hs.498569	Ribosomal protein S2.
9	3591	Hs.119598	Ribosomal protein L3.
8	3192	Hs.703932	Immunoglobulin kappa constant.
8	3192	Hs.586423	Eukaryotic translation elongation factor 1 alpha 1.
8	3192	Hs.5662	Guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1.
8	3192	Hs.5308	Ubiquitin A-52 residue ribosomal protein fusion product 1.
8	3192	Hs.523185	Ribosomal protein L13a.
8	3192	Hs.477028	Ribosomal protein L24.
8	3192	Hs.446574	Thymosin beta 10.
8	3192	Hs.433529	Ribosomal protein S11.
8	3192	Hs.381126	Ribosomal protein S14.
8	3192	Hs.178551	Ribosomal protein L8.
7	2793	Hs.654439	Apolipoprotein E.
7	2793	Hs.652114	Ribosomal protein L28.
7	2793	Hs.546285	Ribosomal protein, large, P0.
7	2793	Hs.523463	Ribosomal protein L27a.
7	2793	Hs.517145	Enolase 1, (alpha).
7	2793	Hs.514196	Ribosomal protein L27.
7	2793	Hs.410817	Ribosomal protein L13.
7	2793	Hs.408073	Ribosomal protein S6.
7	2793	Hs.407995	Macrophage migration inhibitory factor (glycosylation-inhibiting factor).
7	2793	Hs.400295	Ribosomal protein L30.
6	2394	Hs.80545	Ribosomal protein L37.
6	2394	Hs.714487	Transcribed locus, moderately similar to NP_001020329.1 CD74 antigen isoform c [Homo sapiens].
6	2394	Hs.644639	Eukaryotic translation elongation factor 1 alpha 1.
6	2394	Hs.632703	Ribosomal protein L41.
6	2394	Hs.523836	Glutathione S-transferase pi 1.
6	2394	Hs.522632	TIMP metallopeptidase inhibitor 1.
6	2394	Hs.387208	Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed.
6	2394	Hs.381219	Ribosomal protein L15.
6	2394	Hs.381123	Ribosomal protein L21.
6	2394	Hs.378103	Ribosomal protein S5.
6	2394	Hs.374596	Tumor protein, translationally-controlled 1.
6	2394	Hs.356502	Ribosomal protein, large, P1.
6	2394	Hs.304682	Cystatin C.
6	2394	Hs.249184	POU class 5 homeobox 1.
6	2394	Hs.111779	Secreted protein, acidic, cysteine-rich (osteonectin).
5	1995	Hs.706580	Immunoglobulin lambda locus.
5	1995	Hs.627414	Ribosomal protein S18.
5	1995	Hs.592455	Transcribed locus, strongly similar to NP_001003447.1 hypothetical protein LOC445053 [Danio rerio].
5	1995	Hs.546286	Ribosomal protein S3.
5	1995	Hs.534404	Ribosomal protein L10.
5	1995	Hs.511605	Annexin A2.
5	1995	Hs.479728	Transcribed locus, strongly similar to NP_002037.2 glyceraldehyde-3-phosphate dehydrogenase [Homo sapiens].
5	1995	Hs.437594	Ribosomal protein, large, P2.
5	1995	Hs.424126	Small EDRK-rich factor 2.
5	1995	Hs.419463	Ribosomal protein L23a.
5	1995	Hs.181163	High-mobility group nucleosomal binding domain 2.
4	1596	Hs.716390	Similar to 23 kD highly basic protein.
4	1596	Hs.714721	Transcribed locus, strongly similar to NP_997675.1 ribosomal protein L18a [Rattus norvegicus].
4	1596	Hs.714447	Transcribed locus, strongly similar to NP_033106.1 ribosomal protein L26 [Mus musculus].
4	1596	Hs.709313	Beta-2-microglobulin.
4	1596	Hs.702021	Heat shock 70kDa protein 8.
4	1596	Hs.653904	Ribosomal protein L41.
4	1596	Hs.633003	Apolipoprotein A-I.
4	1596	Hs.632717	Myosin, light chain 6, alkali, smooth muscle and non-muscle.
4	1596	Hs.632379	Complement component 1, q subcomponent, A chain.
4	1596	Hs.533624	H3 histone, family 3A.
4	1596	Hs.532634	Interferon, alpha-inducible protein 27.
4	1596	Hs.529631	Ribosomal protein L35a.
4	1596	Hs.525600	Heat shock protein 90kDa alpha (cytosolic), class A member 1.
4	1596	Hs.524910	Ferritin, heavy polypeptide 1.
4	1596	Hs.520640	Actin, beta.
4	1596	Hs.515517	Ribosomal protein L18.
4	1596	Hs.512675	Ribosomal protein S8.
4	1596	Hs.467408	Tripartite motif-containing 28.
4	1596	Hs.436657	Clusterin.
4	1596	Hs.412370	Ribosomal protein L9.
4	1596	Hs.397609	Ribosomal protein S16.
4	1596	Hs.370247	Transcribed locus.
4	1596	Hs.288215	CDNA clone IMAGE:3831740.
4	1596	Hs.247077	Ras homolog gene family, member A.
4	1596	Hs.203717	Fibronectin 1.
4	1596	Hs.183684	Eukaryotic translation initiation factor 4 gamma, 2.
4	1596	Hs.170622	EGF-containing fibulin-like extracellular matrix protein 2.
4	1596	Hs.15535	Ribosomal protein L11.
4	1596	Hs.148340	Ribosomal protein L10a.
4	1596	Hs.112318	Translocase of outer mitochondrial membrane 7 homolog (yeast).
3	1197	Hs.77961	Major histocompatibility complex, class I, B.
3	1197	Hs.716432	Transcribed locus, strongly similar to NP_775369.1 ribosomal protein L36A [Danio rerio].
3	1197	Hs.709403	Transcribed locus, strongly similar to NP_031501.1 ribosomal protein, large, P0 [Mus musculus].
3	1197	Hs.709223	Ribosomal protein S2.
3	1197	Hs.708256	Transcribed locus, moderately similar to NP_989488.2 eukaryotic translation elongation factor 1 alpha 1 [Gallus gallus].
3	1197	Hs.69855	Cold shock domain containing E1, RNA-binding.
3	1197	Hs.655579	Transcribed locus, strongly similar to NP_001021.1 ribosomal protein S27 [Homo sapiens].
3	1197	Hs.645643	Transcribed locus, strongly similar to XP_867599.1 PREDICTED: similar to 40S ribosomal protein SA (p40) (34/67 kDa laminin receptor) (Colon carcinoma laminin-binding protein) (NEM/1CHD4) (Multidrug resistance-associated protein MGr1-Ag) isoform 3 [Canis familiaris].
3	1197	Hs.645317	Ribosomal protein S10.