Kotler DP, Garro ML, Giang T, Orenstein JM; International Conference on AIDS.
Int Conf AIDS. 1993 Jun 6-11; 9: 63 (abstract no. WS-B20-2).
St. Luke's-Roosevelt Hosp, Columbia Univ., New York.
OBJECTIVE: Intestinal microsporidiosis (M) is the cause of chronic diarrhea and severe enteropathy in AIDS in up to one third of unexplained cases. To date, diagnosis has relied upon transmission electron microscopy (TEM), due to uncertainties in routine light microscopy (LM). We determined the sensitivity and specificity of the LM diagnosis of M and evaluated mucosal touch preparation (TP) and tissue chromatrope staining (TC) as confirmatory tests. METHODS: A consecutive series of 34 jejunal biopsies from AIDS patients were evaluated independently by LM (H&E) at St. Luke's-Roosevelt (SLR) and George Washington University (GWU), in addition to TP by modified Giemsa staining, TC and TEM. RESULTS: Microsporidosis was diagnosed by TEM in 15 cases. TABULAR DATA, SEE ABSTRACT VOLUME. LM results at SLR and GWU agreed in 30/34 cases, but diagnosis was difficult in 5 cases from GWU and 8 from SLR. M was characteristic on TP and TC, and no false + were seen. CONCLUSIONS: Pathologists with experience in biopsies from AIDS patients should be able to diagnose M on H&E in the majority of cases, 2) TP and TC are useful adjuncts and can provide rapid confirmation of LM diagnosis, 3) TEM should be needed for only the small minority of cases of M.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Biopsy
- Diarrhea
- Humans
- Intestinal Diseases
- Intestinal Diseases, Parasitic
- Light
- Microsporidiosis
- Phototherapy
- Sensitivity and Specificity
- Staining and Labeling
- Washington
- diagnosis
- instrumentation
Other ID:
UI: 102205643
From Meeting Abstracts