|
| Status |
Public on May 01, 2008 |
| Title |
CRU950Cy3_RefCy5 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source Name |
CRU950
|
| Organism(s) |
Homo sapiens |
| Characteristics |
Alveolar macrophages from subject CRU950 treated with 1 ug/mL Chapel Hill PM2.5 particles for 4 hrs.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
Low Input Linear Amplification Kit (Agilent)
|
| |
|
| Channel 2 |
| Source Name |
Reference pool
|
| Organism(s) |
Homo sapiens |
| Characteristics |
Alveolar macrophages from 6 subjects included in the study. Macrophages were treated with vehicle control.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy5
|
| Label protocol |
Low Input Linear Amplification Kit (Agilent)
|
| |
|
| |
| Hybridization protocol |
standard Agilent hybridization
|
| Scan protocol |
Axon 4000B scanner
|
| Description |
alveolar macrophages from 6 subjects were treated in vitro with PM2.5 particles or vehicle control for 4 hrs
|
| Data processing |
Feature extraction done in TIGR Spotfinder and lowess normalization done in TIGR MIDAS.
|
| |
|
| Submission date |
Feb 05, 2008 |
| Contact name |
Ivana V Yang |
| E-mail(s) |
yangi@njhealth.org
|
| Phone |
303-270-2589
|
| Fax |
303-270-2136
|
| Organization name |
National Jewish Health
|
| Department |
Medicine
|
| Street address |
1400 Jackson Street
|
| City |
Denver |
| State/province |
CO |
| ZIP/Postal code |
80206 |
| Country |
USA |
| |
|
| Platform ID |
GPL6458 |
| Series (1) |
| GSE10394 |
INDUCTION OF OXIDATIVE STRESS AND METAL BINDING GENES IN HUMAN ALVEOLAR MACROPHAGES EXPOSED TO FINE PARTICLES |
|
