Soares_NhHMPu_S1

Library Description

Organism:	Homo sapiens
Organ:	mixed (see below)
Tissue:	Pooled human melanocyte, fetal heart, and pregnant uterus
Vector:	pT7T3D-PacI
Vector type:	phagemid (ampicillin resistant)
Cloning site 1:	Not I
Cloning site 2:	Eco RI
Lab host:	DH10B

Equal amounts of plasmid DNA from three normalized libraries (melanocyte 2NbHM, pregnant uterus NbHPU, and fetal heart NbHH19W) were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 3 libraries. The pools consisted of I.M.A.G.E. clones 260232-265223, 340488-345479, and 484488-489479.

Source: Wilson RK, Washington University School of Medicine

Gene Content Analysis

39,765 ESTs from this library were grouped into 12,805 UniGene entries (putative genes) [UniGene build #217, 04-Dec-2008]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
38	956	Hs.308074	CDNA clone MGC:195946 IMAGE:9093277.
35	880	Hs.155742	Glyoxylate reductase/hydroxypyruvate reductase, mRNA (cDNA clone MGC:3078 IMAGE:3347232).
33	830	Hs.500842	Meningioma expressed antigen 5 (hyaluronidase), mRNA (cDNA clone MGC:48750 IMAGE:5558183).
33	830	Hs.164226	(clone lambda-TS-33) thrombospondin (THBS) mRNA, 5' end.
33	830	Hs.154163	Protein arginine methyltransferase 2, mRNA (cDNA clone IMAGE:5270398).
31	780	Hs.484363	MRNA; cDNA DKFZp547C1510 (from clone DKFZp547C1510).
31	780	Hs.370781	KIAA0251 gene.
31	780	Hs.355934	Myoblast cell surface antigen 24.1D5.
30	754	Hs.446240	MRNA upregulated during camptothecin-induced apoptosis of U937 cells.
30	754	Hs.380627	CKLF-like MARVEL transmembrane domain containing 6, mRNA (cDNA clone IMAGE:3948216).
30	754	Hs.369017	Rab2 mRNA, YPT1-related and member of ras family.
30	754	Hs.297304	Glycosyltransferase 8 domain containing 1 (GLT8D1), transcript variant 1, mRNA.
28	704	Hs.699195	SOX-4 protein.
28	704	Hs.370247	Transcribed locus.
27	679	Hs.716433	Protein phosphatase 2, regulatory subunit B', alpha isoform, mRNA (cDNA clone MGC:26270 IMAGE:4815091).
27	679	Hs.715644	Transcribed locus, strongly similar to NP_058878.1 H2A histone family, member Y [Rattus norvegicus].
27	679	Hs.130438	ODZ3 (ODZ3).
26	654	Hs.62886	CDNA FLJ52396 complete cds, highly similar to SPARC-like protein 1 precursor.
26	654	Hs.591633	KIAA0045.
26	654	Hs.497788	MRNA fragment for glutaminyl-tRNA synthetase (EC 6.1.1.18).
26	654	Hs.477879	H2A.X mRNA encoding histone H2A.X.
25	629	Hs.708303	Transcribed locus, strongly similar to NP_002308.2 lysyl oxidase preproprotein [Homo sapiens].
25	629	Hs.602757	Clone 24498 RNA polymerase II 140 kDa subunit.
25	629	Hs.567438	Brain protein I3, mRNA (cDNA clone MGC:31865 IMAGE:4875257).
25	629	Hs.493919	PZR isoform b (MPZL1) mRNA, complete cds; alternatively spliced.
25	629	Hs.335513	CDNA FLJ53487 complete cds, highly similar to Coagulation factor XIII A chain precursor (EC 2.3.2.13).
25	629	Hs.23871	CDNA clone IMAGE:30924414.
25	629	Hs.210532	KIAA0141 gene.
25	629	Hs.143873	Calpactin I light chain, 5'UTR region.
24	604	Hs.372541	Clone IMAGE:30181 mRNA sequence.
23	578	Hs.654572	LAMA3 mRNA for laminin Ah.
23	578	Hs.460317	Senataxin, mRNA (cDNA clone IMAGE:5580661).
23	578	Hs.439060	Full length insert cDNA clone ZE03C06.
23	578	Hs.2210	Thyroid hormone receptor interacting protein 3.
23	578	Hs.203717	Migration stimulation factor FN70+ (FN gene).
23	578	Hs.162233	Chromodomain helicase DNA binding protein 4, mRNA (cDNA clone MGC:46187 IMAGE:5528023).
23	578	Hs.131711	Dihydrolipoamide dehydrogenase.
23	578	Hs.120	Peroxiredoxin 6, mRNA (cDNA clone MGC:46173 IMAGE:5767682).
23	578	Hs.102406	CDNA FLJ60288 complete cds, highly similar to Melanophilin.
22	553	Hs.714890	PREDICTED: Homo sapiens hypothetical LOC387763 (LOC387763), mRNA.
22	553	Hs.714296	CDNA PSEC0103 fis, clone NT2RP2004755, highly similar to Reticulon-3.
22	553	Hs.558799	Proteasome subunit C8.
22	553	Hs.527524	WWC family member 3 (WWC3), mRNA.
22	553	Hs.493819	CDNA FLJ90007 fis, clone HEMBA1000128, weakly similar to PATHOGENESIS-RELATED PROTEIN 1 PRECURSOR.
22	553	Hs.471779	LRR FLI-I interacting protein 1 (LRRFIP1).
22	553	Hs.432330	Adenovirus protein E3-14.7k interacting protein 1 (FIP-1).
22	553	Hs.36587	CDNA FLJ57350 complete cds, highly similar to Protein phosphatase 1 regulatory subunit 7.
21	528	Hs.714791	Jun oncogene, mRNA (cDNA clone IMAGE:3947905).
21	528	Hs.528834	Pre-mRNA cleavage factor I 25 kDa subunit.
21	528	Hs.524250	CDNA FLJ59143 complete cds, highly similar to Gamma-aminobutyric acid receptor-associated protein-like 1.
21	528	Hs.517307	CDNA FLJ35689 fis, clone SPLEN2019379, highly similar to INTERFERON-REGULATED RESISTANCE GTP-BINDING PROTEIN MXA.
21	528	Hs.513984	Flightless I homolog (Drosophila), mRNA (cDNA clone IMAGE:3915427).
21	528	Hs.471200	Vascular endothelial cell growth factor 165 receptor 2 (VEGF165R2).
21	528	Hs.380897	CDNA FLJ57463 complete cds, highly similar to Fused toes protein.
21	528	Hs.349656	Scavenger receptor class B, member 2, mRNA (cDNA clone IMAGE:5262974).
21	528	Hs.325846	Zinc finger CCCH-type containing 14, mRNA (cDNA clone IMAGE:4904207).
21	528	Hs.268787	MOP-6.
20	503	Hs.714727	Makorin ring finger protein 1, mRNA (cDNA clone MGC:35287 IMAGE:5176176).
20	503	Hs.6232	CDNA FLJ57460 complete cds, highly similar to STAGA complex 65 subunit gamma.
20	503	Hs.522114	CDNA FLJ60566 complete cds, highly similar to Clathrin light chain A.
20	503	Hs.502876	RhoB gene mRNA.
20	503	Hs.491440	Phosphatase 2A.
20	503	Hs.472330	Chromosome 20 open reading frame 3 (C20orf3), mRNA.
20	503	Hs.406530	CDNA FLJ60203 complete cds.
20	503	Hs.356604	KIAA0344 gene.
20	503	Hs.155942	Homo sapiens, clone IMAGE:4332660, mRNA.
19	478	Hs.97858	KIAA0591 protein.
19	478	Hs.78948	Rab geranylgeranyl transferase, beta-subunit.
19	478	Hs.716514	CDNA clone IMAGE:5269868.
19	478	Hs.555910	TOX high mobility group box family member 4, mRNA (cDNA clone IMAGE:4721305).
19	478	Hs.532755	CDNA FLJ58848 complete cds, highly similar to Homo sapiens transcription factor IIB.
19	478	Hs.527861	OS-9.
19	478	Hs.515785	Biliverdin-IXbeta reductase I.
19	478	Hs.487046	CDNA clone IMAGE:5301545.
19	478	Hs.472437	CDNA FLJ52068 complete cds, highly similar to Microtubule-associated protein RP/EB family member 1.
19	478	Hs.444451	Mixed lineage kinase.
19	478	Hs.42146	PAX3B=transcription factor {alternatively spliced} [human, adult cerebellum, mRNA, 841 nt].
19	478	Hs.408355	CDNA FLJ51289 complete cds, highly similar to Low-density lipoprotein receptor-related protein 11 precursor.
19	478	Hs.282901	Clone DT1P1F10 mRNA, CAG repeat region.
19	478	Hs.2533	CDNA FLJ51658 complete cds, highly similar to 4-trimethylaminobutyraldehyde dehydrogenase (EC 1.2.1.47).
19	478	Hs.2178	CDNA FLJ36428 fis, clone THYMU2011564, highly similar to HISTONE H2B GL105.
19	478	Hs.170107	CDNA FLJ20218 fis, clone COLF3345.
19	478	Hs.165859	Clone IMAGE 286356.
19	478	Hs.163893	PICALM mRNA for phosphatidylinositol-binding clathrin assembly protein isoform 2, complete cds, clone: FLJ08165AAAN.
19	478	Hs.124503	Zinc finger homeodomain protein.
18	453	Hs.654792	Clone 24497 mRNA sequence.
18	453	Hs.591706	Tetraspanin 5, mRNA (cDNA clone MGC:9300 IMAGE:3895933).
18	453	Hs.571177	RRM RNA binding protein Gry-rbp (GRY-RBP).
18	453	Hs.531085	Hs-TBX2=T-box gene {T-box region} [human, fetal kidney, mRNA Partial, 283 nt].
18	453	Hs.389700	Microsomal glutathione S-transferase 1 (MGST1), transcript variant 1d, mRNA.
18	453	Hs.289092	Autonomously replicating sequence (ARS) mRNA.
18	453	Hs.27695	Ring finger protein (FXY).
18	453	Hs.274415	PQ loop repeat containing 3 (PQLC3), mRNA.
18	453	Hs.141125	Cysteine protease Yama.
18	453	Hs.124027	CDNA FLJ57394 complete cds, highly similar to Selenide, water dikinase 1 (EC 2.7.9.3).
17	428	Hs.716436	CDNA FLJ90227 fis, clone NT2RM1000899, highly similar to Mitochondrial solute carrier protein.
17	428	Hs.706793	Clone 334-2C mRNA sequence.
17	428	Hs.694727	Serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1, mRNA (cDNA clone MGC:20155 IMAGE:3531777).
17	428	Hs.6909	Monooxygenase, DBH-like 1 (MOXD1), transcript variant 2, mRNA.
17	428	Hs.655519	Myopodin.