LUFT BJ, YANG X, QIU D, DATTWYLER R, DUNN J; Interscience Conference on Antimicrobial Agents and Chemotherapy.
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2000 Sep 17-20; 40: 248.
State Univ. of New York, Stony Brook, NY
BACKGROUND: A major limitation of the current OspA vaccine for Lyme disease is that it is directed against an antigen expressed predominantly in the tick vector. Another immunoprotective antigen is OspC which is expressed in early infection. We have bioengineered a chimeric vaccine containing epitopes derived from OspA and OspC.METHODS: Chimeric forms of the OspA and OspC proteins were bioengineered such that the conformation of the protective domains of each protein were maintained. Mice were immunized with the chimeric vaccine candidate in alum. Antibody from immunized mice were tested for reactivity against OspA and OspC in an ELISA and for cytotoxicity against B.burgdorferi in a complement-dependent assay. Mice were challenged with ticks infected with Borrelia burgdorferi and transmission of infection was assessed by seroconversion and culture.RESULTS: Mice immunized with the chimeric antigens gave a remarkable and equivalent antibody response to both OspA and OspC compared to the lipidated OspA and OspC antigen when used alone. In addition, the antibody in these sera were also cytotoxic against two different strains of Borrelia burgdorferi that were predominantly expressing either OspA or OspC. 10 of 10 chimer immunized mice (p<0.001) were fully protected as measured by seroconversion and culture against challenge with ticks infected with Borrelia burgdorferi compared to sham vaccinated controls (100% infected).CONCLUSIONS: Mice immunized with OspA-OspC chimeric proteins generate a potent protective immune response against two targets that are expressed at different points of the life cycle of Borrelia burgdorferi. This approach has important advantages compared to the current vaccine strategies for Lyme disease.KEYWORDS: Lyme disease; Outer surface protein; Vaccine
Publication Types:
Keywords:
- 1-oleoyl-2-stearoylphosphatidylcholine
- Animals
- Antigens, Bacterial
- Antigens, Surface
- Bacterial Outer Membrane Proteins
- Borrelia burgdorferi
- Borrelia burgdorferi Group
- Enzyme-Linked Immunosorbent Assay
- Epitopes
- Lipoproteins
- Lyme Disease
- Lyme Disease Vaccines
- Mice
- OspA protein
- OspC protein
- Phosphatidylcholines
- Ticks
- immunology
Other ID:
UI: 102249049
From Meeting Abstracts