|
| Status |
Public on Jun 27, 2008 |
| Title |
Non-CpG DNA at 6h-3 |
| Sample type |
RNA |
| |
|
| Source Name |
primary mesangial cells, Non-CpG DNA at 6h-3
|
| Organism(s) |
Mus musculus |
| Characteristics |
Genotype: C57Bl/6
Age: 8-10 week old
|
| Treatment protocol |
primary mesangial cells treated with non CpG DNA and 3P-RNA for 6 hours
|
| Growth protocol |
Primary mesangial cells were isolated from kidney and grow them in RPMI 1640 containing 20% FCS, 1% ITS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using RNAeasy Mini Kit according manufacturer-suggested protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix Expresssion Analysis Technical Manual from 6 µg total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, we used standard Affymetrix procedures for hybridization
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Gene expression data from primary mesangial cells that are stimulated with Non-CpG DNA and 3P-RNA
|
| Data processing |
The data were analyzed with RMA Express version 1.0 beta 2
|
| |
|
| Submission date |
Jun 26, 2008 |
| Contact name |
Ramanjaneyulu Allam |
| E-mail(s) |
Allam.Ramanjangyulu@med.uni-muenchen.de
|
| Organization name |
Klinikum der Universität München
|
| Department |
Medizinische Poliklinik-Innenstadt
|
| Street address |
Schillerstr. 42
|
| City |
munich |
| ZIP/Postal code |
80336 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE11898 |
Expression data from primary mesangial cells stimulated with DNA and RNA ligands |
|
