Fetal heart

Library Description

Organism:	Homo sapiens
Vector:	Lambda gt22
Vector type:	Phage
Cloning site 1:	NotI
Cloning site 2:	SalI
Lab host:	E. coli Y1090

mRNA was purified from human fetal hearts (10-12 weeks). cDNA was constructed using a NotI-Oligo dT adaptor-primer. SalI adaptors were ligated, followed by digestion with NotI, for direction cloning into predigested lambda gt22. Method is described in J. Mol. Cell. Cardiol. (1994) 26, 1329-1333)

Source: Liew CC, Harvard Medical School

Gene Content Analysis

1,366 ESTs from this library were grouped into 996 UniGene entries (putative genes) [UniGene build #217, 04-Dec-2008]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
40	29283	Hs.644639	Eukaryotic translation elongation factor 1 alpha 1, mRNA (cDNA clone MGC:9345 IMAGE:3461588).
40	29283	Hs.586423	MRNA fragment for elongation factor TU (N-terminus).
13	9517	Hs.75640	Natriuretic peptide precursor A (NPPA), mRNA.
10	7321	Hs.712539	Gamma-A globin.
10	7321	Hs.134602	Titin.
8	5857	Hs.83577	Cysteine and glycine-rich protein 3 (cardiac LIM protein) (CSRP3), transcript variant 2, mRNA.
7	5124	Hs.701394	Transcribed locus, moderately similar to NP_989488.2 eukaryotic translation elongation factor 1 alpha 1 [Gallus gallus].
7	5124	Hs.544577	Glyceraldehyde-3-phosphate dehydrogenase, mRNA (cDNA clone MGC:23503 IMAGE:4842418).
7	5124	Hs.443687	Four and a half LIM domains 2, mRNA (cDNA clone MGC:15296 IMAGE:3840517).
6	4392	Hs.643483	Calmodulin.
6	4392	Hs.533613	Troponin T cardiac isoform mRNA, 3' end of cds.
6	4392	Hs.170839	Phospholamban, mRNA (cDNA clone MGC:12311 IMAGE:3934199).
6	4392	Hs.136348	Extracellular matrix protein periostin-bm.
6	4392	Hs.133892	Alpha-tropomyosin.
5	3660	Hs.678918	Beta-MHC mRNA for beta myosin heavy chain clone gtMHC-V.
5	3660	Hs.446123	CDNA FLJ50113 complete cds, highly similar to F-actin capping protein alpha-2 subunit.
5	3660	Hs.406475	Lumican, mRNA (cDNA clone MGC:32797 IMAGE:4688606).
5	3660	Hs.302145	CDNA clone IMAGE:3930644.
4	2928	Hs.709351	CDNA FLJ78096 complete cds, highly similar to Homo sapiens actin, alpha, cardiac muscle (ACTC), mRNA.
4	2928	Hs.654651	Full length insert cDNA clone ZD82E12.
4	2928	Hs.57975	Cardiac calsequestrin.
4	2928	Hs.546285	(xs13) mRNA, 284bp.
4	2928	Hs.526521	CDNA FLJ52206 complete cds, highly similar to Malate dehydrogenase, cytoplasmic (EC 1.1.1.37).
4	2928	Hs.446149	Lactate dehydrogenase B (LDH-B).
4	2928	Hs.418167	Serum albumin (HSA).
4	2928	Hs.369430	Clone 23731 peptidylglycine alpha-amidating monooxygenase.
4	2928	Hs.334347	Creatine kinase M.
4	2928	Hs.320890	MRNA; cDNA DKFZp451O223 (from clone DKFZp451O223).
4	2928	Hs.156316	EST from clone 130486, 5' end.
3	2196	Hs.78771	MRNA encoding phosphoglycerate kinase.
3	2196	Hs.716463	Cytochrome b small subunit of complex II.
3	2196	Hs.714548	Transcribed locus.
3	2196	Hs.712735	(xs31) mRNA, 835bp.
3	2196	Hs.656031	Popeye domain containing 2, mRNA (cDNA clone IMAGE:4517469).
3	2196	Hs.594952	Mutant desmin.
3	2196	Hs.593422	Putative cytosolic NADP-dependent isocitrate dehydrogenase.
3	2196	Hs.5662	G protein beta subunit-like protein 12.3, 5'UTR region.
3	2196	Hs.546283	Ribosomal protein L6.
3	2196	Hs.534603	PREDICTED: Homo sapiens similar to QM protein, transcript variant 2 (LOC284393), mRNA.
3	2196	Hs.524390	Alpha-tubulin {EST} [human, mRNA Partial, 337 nt].
3	2196	Hs.519904	RNA binding motif protein 24, mRNA (cDNA clone IMAGE:4151433).
3	2196	Hs.518475	EIF4A2=protein synthesis initiation factor 4A-II homolog [human, fetal lung tissue, mRNA Partial, 1881 nt].
3	2196	Hs.518084	CDNA FLJ32247 fis, clone PROST1000120.
3	2196	Hs.507498	Mitochondrial intermediate peptidase precursor (MIPEP) mRNA, nuclear gene for mitochondrial product.
3	2196	Hs.473583	DbpB-like protein.
3	2196	Hs.444403	CDNA FLJ40942 fis, clone UTERU2008516.
3	2196	Hs.436803	Von Hippel-Lindau binding protein (VBP-1).
3	2196	Hs.435369	Skeletal muscle LIM-protein SLIM.
3	2196	Hs.412370	CDNA FLJ52179 complete cds, highly similar to 60S ribosomal protein L9.
3	2196	Hs.408767	Alpha B-crystallin=Rosenthal fiber component [human, glioma cell line, mRNA, 691 nt].
3	2196	Hs.374503	Putative transcription factor MRG15-2 (MRG15) mRNA, complete cds; alternatively spliced.
3	2196	Hs.227752	CDNA FLJ75326 complete cds.
3	2196	Hs.172928	COL1A1=type I collagen pro alpha 1(I) chain propeptide {3' region} [human, fetal cells 86-237, 86-146, 88-251, mRNA Partial Mutant, 855 nt].
3	2196	Hs.144835	CDNA FLJ59973 complete cds, highly similar to Elongation factor 1-gamma.
3	2196	Hs.144114	Calcium/calmodulin-dependent protein kinase II delta (CAMK2D), transcript variant 4, mRNA.
2	1464	Hs.90436	Sperm associated antigen 7, mRNA (cDNA clone IMAGE:4303011).
2	1464	Hs.77060	Proteasome (prosome, macropain) subunit, beta type, 6 (PSMB6), mRNA.
2	1464	Hs.75636	Myosin, light chain 7, regulatory, mRNA (cDNA clone MGC:34234 IMAGE:5223298).
2	1464	Hs.75535	Cardiac ventricular myosin light chain 2.
2	1464	Hs.716403	Homolog of Nedd5 (hNedd5).
2	1464	Hs.716377	CDNA FLJ90489 fis, clone NT2RP3003473, moderately similar to PAI-1 mRNA-binding protein.
2	1464	Hs.715549	Transcribed locus, strongly similar to NP_031501.1 ribosomal protein, large, P0 [Mus musculus].
2	1464	Hs.713159	Transcribed locus, strongly similar to NP_001011995.1 tubulin, alpha 1C [Rattus norvegicus].
2	1464	Hs.708738	(xs163) mRNA, 390bp.
2	1464	Hs.708162	Transcribed locus.
2	1464	Hs.707724	Transcribed locus, strongly similar to NP_000961.2 ribosomal protein L6 [Homo sapiens].
2	1464	Hs.706312	Transcribed locus, strongly similar to NP_001003447.1 hypothetical protein LOC445053 [Danio rerio].
2	1464	Hs.706089	CDNA FLJ33826 fis, clone CTONG2003427.
2	1464	Hs.703113	Transcribed locus.
2	1464	Hs.702021	Heat shock 70kDa protein 8 (HSPA8), transcript variant 2, mRNA.
2	1464	Hs.702010	Full length insert cDNA clone YP80G10.
2	1464	Hs.701778	MRNA; cDNA DKFZp686L0695 (from clone DKFZp686L0695); complete cds.
2	1464	Hs.699301	Voltage dependent anion channel form 3.
2	1464	Hs.694860	Transcribed locus, strongly similar to NP_001135947.1 neuronal protein 3.1 isoform c [Homo sapiens].
2	1464	Hs.657308	Transcribed locus, weakly similar to XP_906188.3 PREDICTED: hypothetical protein [Mus musculus].
2	1464	Hs.657243	Transcribed locus.
2	1464	Hs.655384	Transcribed locus.
2	1464	Hs.655143	Arg/Abl-interacting protein ArgBP2a (ArgBP2a).
2	1464	Hs.654638	CDNA FLJ76566 complete cds, highly similar to Homo sapiens putative NFkB activating protein, mRNA.
2	1464	Hs.651219	NADH:ubiquinone oxidoreductase subunit B13.
2	1464	Hs.648385	Putative HIV-1 infection related protein.
2	1464	Hs.645344	Transcribed locus, strongly similar to NP_775462.2 ribosomal protein L13A [Rattus norvegicus].
2	1464	Hs.636480	MRNA fragment encoding beta-tubulin. (from clone D-beta-1).
2	1464	Hs.632717	Smooth muscle myosin alkali light chain.
2	1464	Hs.632287	CAMP dependent proteinkinase PRKY, clone ct4A1.
2	1464	Hs.628678	Vimentin.
2	1464	Hs.620557	Ankyrin, Brank-1 protein.
2	1464	Hs.592048	Cytochrome bc-1 complex core protein II.
2	1464	Hs.586401	Transcribed locus.
2	1464	Hs.567598	CDNA FLJ34887 fis, clone NT2NE2016971, moderately similar to XLCL2 protein.
2	1464	Hs.567412	Leucine rich repeat containing 17, mRNA (cDNA clone MGC:34451 IMAGE:5229061).
2	1464	Hs.555915	Full length insert cDNA clone ZD97A03.
2	1464	Hs.546269	Csa-19.
2	1464	Hs.54457	CD81 molecule, mRNA (cDNA clone MGC:2221 IMAGE:3510634).
2	1464	Hs.533732	Signal recognition particle 14kDa (homologous Alu RNA binding protein), mRNA (cDNA clone MGC:14326 IMAGE:4298087).
2	1464	Hs.533526	Putative DNA dependent ATPase and helicase (ATRX) mRNA, alternatively spliced product 3.
2	1464	Hs.532359	Ribosomal protein L5.
2	1464	Hs.531561	Epithelial membrane protein-2.
2	1464	Hs.5308	MRNA fragment for ubiquitin (C-terminus).
2	1464	Hs.5258	Melanoma antigen family D, 1, mRNA (cDNA clone MGC:19900 IMAGE:4026470).