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An organized view of the transcripome



Library:5176 (dbEST ID)

HT0495

Library Description

Organism:Homo sapiens
Developmental stage:Adult
Organ:head_neck
Vector:puc18
Vector type:Plasmid
Cloning site 1:SmaI
Cloning site 2:SmaI

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

53 ESTs from this library were grouped into 44 UniGene entries (putative genes) [UniGene build #217, 04-Dec-2008]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs TPM   UniGene Entry
3 56604 Hs.499839 Ribosomal protein L7a, mRNA (cDNA clone MGC:23150 IMAGE:4841982).
2 37736 Hs.7744 NDUFV1=NADH:ubiquinone oxidoreductase flavoprotein 1 subunit [human, kidney, mRNA Partial, 771 nt].
2 37736 Hs.520640 MRNA fragment encoding cytoplasmic actin. (isolated from cultured epidermal cells grown from human foreskin).
2 37736 Hs.495656 Transducin (beta) like 1 protein.
2 37736 Hs.434980 Amyloid protein (AD-AP) mRNA, 3' end.
2 37736 Hs.362806 G protein-coupled receptor 116 (GPR116), transcript variant 1, mRNA.
2 37736 Hs.337295 Stress-induced-phosphoprotein 1, mRNA (cDNA clone MGC:39562 IMAGE:4830381).
2 37736 Hs.127897 Rap guanine nucleotide exchange factor (GEF) 1, mRNA (cDNA clone MGC:49019 IMAGE:6051007).
1 18868 Hs.88025 CDNA FLJ23613 fis, clone ADKA03214.
1 18868 Hs.82890 Defender against cell death 1, mRNA (cDNA clone MGC:13634 IMAGE:4284779).
1 18868 Hs.82071 Msg1-related gene 1 (mrg1).
1 18868 Hs.79136 Solute carrier family 39 (zinc transporter), member 6, mRNA (cDNA clone MGC:50406 IMAGE:5552785).
1 18868 Hs.76152 Uterine water channel=28 kda erythrocyte integral membrane protein homolog [human, uterus, mRNA, 1340 nt].
1 18868 Hs.716383 DEAD-box protein p72 (P72).
1 18868 Hs.712666 MRNA; cDNA DKFZp686K2379 (from clone DKFZp686K2379).
1 18868 Hs.710484 PREDICTED: Homo sapiens hypothetical protein LOC100131476 (LOC100131476), mRNA.
1 18868 Hs.709313 Beta 2- mu =beta 2-microglobulin [human, SK-MEL-33 cells, mRNA Mutant, 433 nt].
1 18868 Hs.659631 Transcribed locus.
1 18868 Hs.654591 Thyroglobulin.
1 18868 Hs.646575 26S proteasome subunit p55.
1 18868 Hs.644639 Eukaryotic translation elongation factor 1 alpha 1, mRNA (cDNA clone MGC:9345 IMAGE:3461588).
1 18868 Hs.636600 Transcribed locus.
1 18868 Hs.632348 Clone IMAGE:322502 L amino acid transporter-2 (SLC7A8).
1 18868 Hs.631827 CDNA FLJ55060 complete cds, highly similar to Annexin A7.
1 18868 Hs.621695 Transcribed locus, moderately similar to NP_055634.3 praja 2, RING-H2 motif containing [Homo sapiens].
1 18868 Hs.6076 JAB1-containing signalosome subunit 3 (SGN3).
1 18868 Hs.598958 Hypothetical gene supported by AK128398, mRNA (cDNA clone IMAGE:5266032).
1 18868 Hs.592225 CDNA FLJ75682 complete cds, highly similar to Homo sapiens armadillo repeat containing, X-linked 3 (ARMCX3), transcript variant 1, mRNA.
1 18868 Hs.587278 Homo sapiens, clone IMAGE:5209126, mRNA.
1 18868 Hs.536663 CDNA FLJ34276 fis, clone FEBRA2003520.
1 18868 Hs.521640 XP-C repair complementing protein (p58/HHR23B).
1 18868 Hs.518265 Transcribed locus.
1 18868 Hs.507515 CDNA FLJ54313 complete cds, highly similar to Homo sapiens olfactomedin-like 2B (OLFML2B), mRNA.
1 18868 Hs.504237 STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A), mRNA.
1 18868 Hs.483993 MRNA full length insert cDNA clone EUROIMAGE 240269.
1 18868 Hs.480364 Methionyl aminopeptidase 1 (METAP1), mRNA.
1 18868 Hs.479693 Arginine-rich nuclear protein.
1 18868 Hs.471851 High density lipoprotein binding protein (vigilin), mRNA (cDNA clone IMAGE:3920936).
1 18868 Hs.465818 Myosin IF (MYO1F), mRNA.
1 18868 Hs.461086 Uvomorulin (put. CAM 120/80).
1 18868 Hs.446260 Proteasome (prosome, macropain) subunit, alpha type, 6, mRNA (cDNA clone MGC:29618 IMAGE:4869452).
1 18868 Hs.435952 CDNA FLJ53994 complete cds, highly similar to CDK5 regulatory subunit-associated protein 1.
1 18868 Hs.136947 Autoantigen p542.
1 18868 Hs.118076 CDNA FLJ40595 fis, clone THYMU2010705, highly similar to 40S RIBOSOMAL PROTEIN S4, X ISOFORM.

Source: NCBI UniGene

EST Sequences

128 EST sequences from this library have been submitted to the public sequence database as of 04-Dec-2008.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

  • thyroid
  • head and neck tumor
  • adult

Source: NCBI Clone Registry

References

Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).



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