Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences

EMBO J. 1984 September; 3(9): 2197–2205.

PMCID: PMC557666

W. D. O. Hamilton, V. E. Stein, R. H. A. Coutts, and K. W. Buck

Department of Pure and Applied Biology, Imperial College of Science and Technology, London SW7 2BB, UK

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Abstract

The nucleotide sequences of the infectious cloned DNA components of tomato golden mosaic virus (TGMV) have been determined. DNA A (2588 nucleotides) and DNA B (2508 nucleotides) have little sequence homology except for a region of ˜200 bases which is almost identical in the two molecules. Analysis of open reading frames revealed six potential coding regions for proteins of mol. wt. >10 000, four in DNA A and two in DNA B. Possible regulatory signals are identified and a model for bidirectional transcription of the two genome components is presented. Comparison of the nucleotide sequences of the DNAs of TGMV and cassava latent virus (CLV) revealed a fairly close relationship between TGMV DNA A and CLV DNA 1 and a comparatively distant relationship between TGMV DNA B and CLV DNA 2. All the potential coding regions in the TGMV DNAs had counterparts in the CLV DNAs suggesting an overall similarity in genome organisation, but six potential coding regions in the CLV DNAs had no counterparts in the TGMV DNAs. The 200-base region common to the two DNAs of each virus had little sequence homology, except for a highly conserved 33-base sequence potentially capable of forming a stable hairpin structure.

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