RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Escolar(*) ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lepidocybium(*) flavobrunneum(*)
FDA Seafood List Common Name: Escolar(*)
FDA Seafood List Market Name: Escolar(*)
Unique IEF Gel Code: 19950830gel09
Unique Web Code: ech04Srr
Tissue Aliquot Code: lepiflav I94-003 #03 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	29	3.7	0.0238387	6	0.247946	13.7	8.1	4.55	29.9	6.3	0.0223278
2	5.2	125	15.9	0.0141836	6	0.12563	6.9	4.1	5.2	125.1	5.7	0.0124558
3	5.85	231	29.4	0.026517	4	0.191106	10.5	6.2	5.85	230.8	4.1	0.0265887
4	6.55	340	43.3	0.0204675	10	0.329565	18.2	10.7	6.55	338.5	10.2	0.018265
5	7.35	439	55.9	0.0136976	7	0.170658	9.4	5.5	7.35	437.3	7.4	0.0129726
6	8.15	556	70.7	0.0187744	10	0.275772	15.2	9	8.15	555.2	9.6	0.0161667
7	8.45	620	78.9	0.0165312	8	0.213386	11.8	6.9	8.45	618.6	7.9	0.015244
8	8.65	672	85.5	0.0174235	4	0.126721	7	4.1	8.65	672.6	4.3	0.0165075
9	9.3	735	93.5	0.0139248	5	0.133996	7.4	4.4	9.3	734.6	5.4	0.0138766

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	6.084	265	33.8	0.0063319	3	0.0398019	0.3	0.2	6.084	263.3	3.2	0.0069692
2	6.182	281	35.8	0.148164	2	0.66742	5.7	4	6.182	281.6	2.5	0.151911
3	6.273	296	37.8	0.056456	4	0.325468	2.8	2	6.273	296.4	3.5	0.051834
4	6.352	309	39.4	0.0795335	5	0.651221	5.6	3.9	6.352	308.3	4.8	0.0766573
5	6.535	339	43.2	0.0258006	3	0.125194	1.1	0.8	6.535	339	2.9	0.0245562
6	6.612	349	44.5	0.107456	5	1.06712	9.2	6.4	6.612	351.5	4.7	0.128673
7	6.686	358	45.7	0.392923	2	1.56496	13.4	9.4	6.686	357.9	2.4	0.361052
8	6.777	369	47.1	0.439281	3	2.13228	18.3	12.8	6.777	369	2.8	0.433711
9	6.843	377	48.1	0.0535296	2	0.215381	1.8	1.3	6.843	377.1	2.2	0.0562185
10	6.917	386	49.2	0.13752	2	0.623826	5.4	3.8	6.917	385.4	2.4	0.145227
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.983	394	50.3	0.0373466	2	0.137602	1.2	0.8	6.983	394	2.1	0.0372878
12	7.065	404	51.5	0.079101	3	0.462989	4	2.8	7.065	404	3.2	0.0814463
13	7.206	421	53.7	0.317852	3	1.66391	14.3	10	7.206	421.4	2.9	0.325111
14	7.91	520	66.3	0.266902	3	1.4324	12.3	8.6	7.91	520.2	3	0.266941
15	8.519	636	81.1	0.0266258	13	0.534404	4.6	3.2	8.519	637.6	12.5	0.024086

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	29	3.7	0.0268333	4	0.189189	12.6	7.8	4.55	28.9	4.1	0.0260056
2	5.2	121	15.5	0.0147107	7	0.154254	10.3	6.4	5.2	121.3	7.2	0.0120047
3	5.85	222	28.5	0.023564	4	0.173083	11.5	7.2	5.85	221.9	4.3	0.022776
4	6.55	343	44	0.0180078	8	0.236038	15.7	9.8	6.55	341.9	7.9	0.0168292
5	7.35	438	56.2	0.0120245	8	0.152175	10.1	6.3	7.35	436.6	8.4	0.010267
6	8.15	554	71	0.012886	10	0.197827	13.2	8.2	8.15	555.6	10.1	0.0110292
7	8.45	616	79	0.0146197	7	0.161737	10.8	6.7	8.45	616.1	6.6	0.0137877
8	8.65	668	85.6	0.0166756	5	0.144298	9.6	6	8.65	668.1	5.1	0.0160818
9	9.3	731	93.7	0.0133246	5	0.0932459	6.2	3.9	9.3	730.6	4.5	0.0116466

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	30	3.9	0.0160035	4	0.0964069	0.9	0.7	4.55	29.9	3.6	0.015101
2	5.2	127	16.3	0.0065367	6	0.0626678	0.6	0.4	5.2	126.7	6.2	0.0057446
3	5.85	228	29.3	0.0144174	5	0.121631	1.1	0.9	5.85	227.4	5.1	0.0133878
4	6.214	287	36.9	0.0890549	3	0.462653	4.2	3.2	6.214	286.5	3	0.0883588
5	6.295	300	38.6	0.0393261	4	0.23286	2.1	1.6	6.295	300.3	3.6	0.0365165
6	6.369	312	40.2	0.0509779	5	0.432675	3.9	3	6.369	311.5	4.8	0.0505922
7	6.556	342	44	0.0274838	3	0.131183	1.2	0.9	6.556	342.1	2.7	0.0277358
8	6.655	354	45.6	0.0890329	7	1.33782	12.2	9.4	6.655	357	7.4	0.10259
9	6.704	360	46.3	0.382656	2	0.989281	9	6.9	6.704	360.4	1.8	0.312709
10	6.796	371	47.7	0.418056	2	1.85179	16.8	13	6.796	371.5	2.4	0.428224
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.862	379	48.8	0.0502244	2	0.208797	1.9	1.5	6.862	378.9	2.4	0.0498743
12	6.936	388	49.9	0.125676	2	0.535303	4.9	3.8	6.936	387.6	2.3	0.132272
13	7.002	396	51	0.0350985	2	0.122827	1.1	0.9	7.002	395.8	1.9	0.0371605
14	7.085	406	52.3	0.0595357	3	0.383498	3.5	2.7	7.085	405.7	3.5	0.0624301
15	7.226	423	54.4	0.284069	3	1.36817	12.4	9.6	7.226	423	2.7	0.288453
16	7.35	438	56.4	0.0285411	5	0.253019	2.3	1.8	7.35	439	5.5	0.0260651
17	7.919	522	67.2	0.19452	4	1.22958	11.2	8.6	7.919	521.5	3.6	0.193178
18	8.15	556	71.6	0.0264981	18	0.712832	6.5	5	8.15	548	18	0.0223433
19	8.45	622	80.1	0.0086382	8	0.0983147	0.9	0.7	8.45	623.5	7.6	0.0073467
20	8.65	673	86.6	0.0237681	10	0.366454	3.3	2.6	8.65	673.5	10.1	0.0204575

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	6.094	261	33.9	0.0083674	4	0.0422434	0.4	0.2	6.094	260.6	3.5	0.0067825
2	6.196	278	36.1	0.134513	3	0.670434	5.9	3.7	6.196	277.7	2.8	0.137457
3	6.275	291	37.7	0.0452182	4	0.274238	2.4	1.5	6.275	291.6	3.5	0.0437766
4	6.353	304	39.4	0.0693005	5	0.578748	5.1	3.2	6.353	303.2	4.8	0.0685163
5	6.534	334	43.3	0.0252053	3	0.130901	1.1	0.7	6.534	333.9	2.9	0.0256326
6	6.62	345	44.7	0.0876679	6	1.13188	9.9	6.3	6.62	346.8	5.8	0.110351
7	6.679	352	45.7	0.407841	2	1.29498	11.3	7.2	6.679	352.3	2	0.360459
8	6.779	364	47.2	0.46657	2	1.99931	17.5	11.2	6.779	363.7	2.4	0.462918
9	6.838	371	48.1	0.065352	2	0.188452	1.6	1.1	6.838	371.7	1.7	0.0639056
10	6.914	380	49.3	0.156198	2	0.64948	5.7	3.6	6.914	379.9	2.3	0.158013
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.989	389	50.5	0.0543529	3	0.2559	2.2	1.4	6.989	389	2.6	0.0555107
12	7.073	399	51.8	0.103637	4	0.729995	6.4	4.1	7.073	399	3.9	0.104402
13	7.216	416	54	0.319941	3	1.5941	14	8.9	7.216	416.2	2.8	0.326756
14	7.923	518	67.2	0.294441	4	1.88536	16.5	10.5	7.923	517.8	3.7	0.289373

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	19	2.5	0.0256592	5	0.225124	13	7.1	4.55	20.1	5	0.0253919
2	5.2	110	14.3	0.0095425	6	0.0974225	5.6	3.1	5.2	111.5	5.9	0.0092753
3	5.85	215	28	0.021297	5	0.175166	10.1	5.5	5.85	215	4.9	0.0199835
4	6.55	334	43.5	0.0247061	8	0.330062	19	10.4	6.55	331.9	8.1	0.0231249
5	7.35	428	55.8	0.0179068	6	0.16604	9.6	5.2	7.35	427.8	5.8	0.0162595
6	8.15	550	71.7	0.0172448	8	0.201025	11.6	6.3	8.15	550.6	7.7	0.0147226
7	8.45	616	80.3	0.0198587	8	0.259139	14.9	8.1	8.45	613.9	7.8	0.0188611
8	8.65	667	87	0.0168771	5	0.152474	8.8	4.8	8.65	666.5	5.1	0.0169892
9	9.3	731	95.3	0.0118664	7	0.131093	7.5	4.1	9.3	728.9	6.9	0.0107711

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 76

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 13.700 5.200 6.900 5.850 10.500 6.550 18.200 7.350 9.400 8.150 15.200 8.450 11.800 8.650 7.000 9.300 7.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
6.084 0.300 6.182 5.700 6.273 2.800 6.352 5.600 6.535 1.100 6.612 9.200 6.686 13.400 6.777 18.300 6.843 1.800 6.917 5.400 6.983 1.200 7.065 4.000 7.206 14.300 7.910 12.300 8.519 4.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 15 Return to Lane Navigator
4.550 12.600 5.200 10.300 5.850 11.500 6.550 15.700 7.350 10.100 8.150 13.200 8.450 10.800 8.650 9.600 9.300 6.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.550 0.900 5.200 0.600 5.850 1.100 6.214 4.200 6.295 2.100 6.369 3.900 6.556 1.200 6.655 12.200 6.704 9.000 6.796 16.800 6.862 1.900 6.936 4.900 7.002 1.100 7.085 3.500 7.226 12.400 7.350 2.300 7.919 11.200 8.150 6.500 8.450 0.900 8.650 3.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 20 Return to Lane Navigator
6.094 0.400 6.196 5.900 6.275 2.400 6.353 5.100 6.534 1.100 6.620 9.900 6.679 11.300 6.779 17.500 6.838 1.600 6.914 5.700 6.989 2.200 7.073 6.400 7.216 14.000 7.923 16.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 14 Return to Lane Navigator
4.550 13.000 5.200 5.600 5.850 10.100 6.550 19.000 7.350 9.600 8.150 11.600 8.450 14.900 8.650 8.800 9.300 7.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Escolar(*) ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Escolar(*) ---- Go to Display Gel Lane Navigator.