RFE Fish Species Identification Isoelectric Focusing Tools - Rainbow (Steelhead) Trout PAGPlate IEF Gel 01

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Rainbow (Steelhead) Trout ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Oncorhynchus mykiss
FDA Seafood List Common Name: Rainbow (Steelhead) Trout
FDA Seafood List Market Name: Trout, Rainbow (Steehead)
Unique IEF Gel Code: 19960328gel01
Unique Web Code: stg01Srr
Tissue Aliquot Code: oncomyki I94-009 #01a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	21	1.9	0.321045	10	5.30418	11.4	9.3	3.5	24.4	9.8	0.304925
2	4.55	174	15.6	0.412553	4	2.98227	6.4	5.2	4.55	174.6	4.4	0.380369
3	5.2	262	23.5	0.406941	4	2.91418	6.3	5.1	5.2	261.6	4.3	0.383336
4	5.85	418	37.6	0.841003	3	4.90805	10.6	8.6	5.85	417.9	3.3	0.851405
5	6.55	589	52.9	0.359537	4	2.41541	5.2	4.2	6.55	588.8	4	0.34053
6	6.85	631	56.7	0.243822	7	2.70146	5.8	4.8	6.85	631	7	0.216481
7	7.35	723	65	0.602675	9	8.42464	18.2	14.8	7.35	721.7	8.5	0.557816
8	8.15	881	79.2	0.136421	8	1.81796	3.9	3.2	8.15	880.9	7.8	0.131695
9	8.45	936	84.1	0.235987	6	2.2879	4.9	4	8.45	935.7	5.5	0.233549
10	8.65	972	87.3	0.215728	5	1.84701	4	3.2	8.65	971.2	4.9	0.213726
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.233	1086	97.6	0.293022	11	5.42248	11.7	9.5	9.233	1087.6	10.9	0.280152
12	9.3	1099	98.7	0.816549	4	5.30077	11.4	9.3	9.3	1098.9	4.1	0.722867

PeakCounter: 12

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 17
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 19
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.561	26	2.3	0.95613	6	11.4178	16.2	12.5	3.561	26.3	6.3	1.01693
2	4.173	117	10.5	0.140533	5	1.12728	1.6	1.2	4.173	116.7	5	0.127516
3	4.261	130	11.7	0.249249	4	1.70771	2.4	1.9	4.261	130.7	4.1	0.236795
4	4.601	180	16.2	0.156814	5	1.13821	1.6	1.2	4.601	180.7	4.6	0.140054
5	5.12	251	22.6	0.326742	3	1.80561	2.6	2	5.12	250.7	3.3	0.312755
6	5.837	414	37.2	0.681628	4	4.36684	6.2	4.8	5.837	413.6	3.9	0.636113
7	5.972	447	40.2	0.996242	3	5.71931	8.1	6.3	5.972	446.9	3.3	0.974161
8	6.005	455	40.9	0.852596	3	4.7221	6.7	5.2	6.005	455.5	3.1	0.859014
9	6.066	470	42.2	0.787759	4	5.1718	7.4	5.7	6.066	469.5	3.6	0.817615
10	6.099	478	42.9	0.302502	2	0.924274	1.3	1	6.099	477.3	1.7	0.306486
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.123	484	43.5	1.00811	3	6.22324	8.8	6.8	6.123	484.8	3.4	1.04026
12	6.181	498	44.7	0.118869	2	0.447848	0.6	0.5	6.181	498	2.1	0.122157
13	6.254	516	46.4	0.68807	3	1.77539	2.5	1.9	6.254	514.9	2.9	0.343623
14	6.34	537	48.2	0.222903	18	8.47665	12	9.3	6.34	522.2	18	0.265696
15	6.364	543	48.8	0.296021	1	0.302026	0.4	0.3	6.364	543.4	0.5	0.288036
16	6.401	552	49.6	0.315465	4	1.41694	2	1.6	6.401	552.9	3.7	0.214683
17	6.503	577	51.8	0.419132	2	1.63527	2.3	1.8	6.503	576.4	2.2	0.42894
18	6.668	605	54.4	0.194227	2	0.642116	0.9	0.7	6.668	604.8	2	0.183079
19	6.839	629	56.5	0.148163	3	0.632214	0.9	0.7	6.839	629.1	2.5	0.140014
20	6.957	650	58.4	0.104891	3	0.525347	0.7	0.6	6.957	650.1	2.9	0.101329
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.082	673	60.5	0.0774226	2	0.347934	0.5	0.4	7.082	672.3	2.4	0.0814293
22	7.159	687	61.7	0.504284	3	2.25966	3.2	2.5	7.159	687.4	2.5	0.509164
23	7.202	695	62.4	0.205277	2	0.620319	0.9	0.7	7.202	694.9	1.8	0.195938
24	7.268	707	63.5	0.0215264	11	0.455301	0.6	0.5	7.268	704.6	10.5	0.0244317
25	7.345	721	64.8	0.241627	2	0.932413	1.3	1	7.345	721	2.3	0.229263
26	7.401	732	65.8	0.64898	3	2.9661	4.2	3.3	7.401	731.6	2.6	0.653569
27	7.514	754	67.7	0.0523819	3	0.263575	0.4	0.3	7.514	753.4	2.7	0.054728
28	8.845	1010	90.7	0.155838	9	2.33101	3.3	2.6	8.845	1008.2	8.7	0.151007

PeakCounter: 28

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 18
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 20
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	14	1.3	0.220612	9	3.39136	8.8	6.9	3.5	17	9.2	0.209042
2	4.55	173	15.5	0.455478	5	3.35004	8.7	6.8	4.55	172.9	4.7	0.405694
3	5.2	263	23.6	0.428778	4	3.13696	8.2	6.3	5.2	262.4	4.3	0.407028
4	5.85	417	37.5	0.846342	3	5.00341	13.1	10.1	5.85	417.1	3.3	0.85538
5	6.55	589	52.9	0.389789	4	2.71746	7.1	5.5	6.55	588.9	4.2	0.369309
6	6.85	631	56.7	0.196621	7	2.02326	5.3	4.1	6.85	631	6.6	0.171953
7	7.35	722	64.9	0.664554	8	8.49509	22.2	17.2	7.35	720.2	7.6	0.633047
8	8.15	876	78.7	0.155503	10	2.66509	7	5.4	8.15	875.4	10.1	0.1483
9	8.45	935	84	0.250156	5	2.37058	6.2	4.8	8.45	935.1	5.4	0.245601
10	8.65	972	87.3	0.221917	5	1.92665	5	3.9	8.65	972.2	4.9	0.2224
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1102	99	0.509107	4	3.24793	8.5	6.6	9.3	1101.3	3.6	0.509133

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 19
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 21
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	27	2.4	0.879977	9	14.2405	13	11.4	3.5	25.2	8.9	0.898231
2	4.151	115	10.3	0.172168	6	1.55549	1.4	1.2	4.151	114.2	5.7	0.1532
3	4.254	129	11.6	0.257356	4	1.8179	1.7	1.5	4.254	129.1	4.3	0.239744
4	4.55	169	15.2	0.261561	4	1.83875	1.7	1.5	4.55	168.5	4.4	0.234358
5	4.626	179	16.1	0.173631	7	2.08725	1.9	1.7	4.626	177.1	6.5	0.180094
6	5.132	246	22.1	0.285972	12	6.14631	5.6	4.9	5.132	248.1	11.9	0.290387
7	5.2	255	22.9	0.238348	9	0.365524	0.3	0.3	5.2	252.3	9.1	0.0227796
8	5.85	408	36.7	0.703826	6	7.45756	6.8	6	5.85	409.9	6	0.699111
9	6.007	447	40.2	0.868913	4	6.15879	5.6	4.9	6.007	446.9	4.3	0.809473
10	6.043	456	41	0.699275	3	4.16913	3.8	3.3	6.043	456.5	3.3	0.719941
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.099	470	42.3	0.665015	4	4.45425	4.1	3.6	6.099	469.5	3.6	0.701679
12	6.136	479	43.1	0.269281	2	0.797638	0.7	0.6	6.136	478.3	1.6	0.276977
13	6.16	485	43.6	0.871941	4	5.73706	5.2	4.6	6.16	485.7	3.5	0.921402
14	6.208	497	44.7	0.0967281	2	0.31868	0.3	0.3	6.208	497.5	1.9	0.0933145
15	6.289	517	46.5	0.715522	10	10.1002	9.2	8.1	6.289	518.7	10	0.567529
16	6.381	540	48.6	0.249598	2	0.642491	0.6	0.5	6.381	540.6	1.9	0.19173
17	6.409	547	49.2	0.26025	2	0.740152	0.7	0.6	6.409	546.5	1.6	0.253601
18	6.441	555	49.9	0.305865	3	1.57736	1.4	1.3	6.441	555.7	3	0.292856
19	6.55	582	52.3	0.418489	2	1.61284	1.5	1.3	6.55	581.7	2.3	0.392706
20	6.764	612	55	0.170635	2	0.599397	0.5	0.5	6.764	612	2.1	0.161465
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.85	624	56.1	0.12686	5	0.975392	0.9	0.8	6.85	624.4	4.7	0.117158
22	6.921	637	57.3	0.159511	3	0.717526	0.7	0.6	6.921	636.4	2.8	0.143706
23	7.029	657	59.1	0.0399126	4	0.493181	0.5	0.4	7.029	653.9	4.3	0.0646032
24	7.149	679	61.1	0.0760382	2	0.325091	0.3	0.3	7.149	678.5	2.3	0.0784887
25	7.23	694	62.4	0.497339	3	2.8947	2.6	2.3	7.23	693.7	3.3	0.500439
26	7.285	704	63.3	0.19728	3	1.00021	0.9	0.8	7.285	703.4	2.9	0.197126
27	7.35	716	64.4	0.506542	5	4.64916	4.2	3.7	7.35	715.7	5	0.519671
28	7.423	730	65.6	0.194578	2	0.683263	0.6	0.5	7.423	729.6	2	0.192215
29	7.475	740	66.5	0.679565	3	3.37831	3.1	2.7	7.475	739.7	2.8	0.692911
30	7.584	761	68.4	0.0503113	3	0.300286	0.3	0.2	7.584	759.9	2.9	0.0579922
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.15	870	78.2	0.0474743	18	1.69508	1.5	1.4	8.15	873.2	18	0.0531314
32	8.45	929	83.5	0.174084	8	2.13549	2	1.7	8.45	929	7.5	0.159802
33	8.65	967	87	0.333706	15	7.48377	6.8	6	8.65	964.8	14.5	0.290489
34	8.858	1008	90.6	0.181441	9	3.08644	2.8	2.5	8.858	1007.3	9.4	0.185196
35	9.3	1095	98.5	0.788387	6	7.16464	6.5	5.7	9.3	1093.6	5.7	0.712592

PeakCounter: 35

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 20
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 22
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.507	24	2.2	0.787257	8	11.6957	16.4	12.7	3.507	27.6	8	0.826941
2	4.181	118	10.6	0.151063	8	1.83937	2.6	2	4.181	115.5	8.2	0.126317
3	4.281	132	11.9	0.234718	3	1.18804	1.7	1.3	4.281	132.6	3.1	0.215483
4	4.653	183	16.5	0.159858	5	1.32444	1.9	1.4	4.653	182.6	5	0.149995
5	5.189	253	22.8	0.324977	3	1.70216	2.4	1.8	5.189	252.4	3	0.316063
6	5.856	409	36.8	0.256844	2	1.01166	1.4	1.1	5.856	409.5	2.1	0.270536
7	5.884	416	37.4	0.582591	3	3.27299	4.6	3.5	5.884	415.7	3.3	0.557045
8	5.983	441	39.7	0.251783	1	0.51545	0.7	0.6	5.983	440.7	1.4	0.209344
9	6.011	448	40.3	0.82875	3	3.86443	5.4	4.2	6.011	447.9	2.6	0.83816
10	6.046	457	41.1	0.721912	3	4.07231	5.7	4.4	6.046	456.8	3.3	0.700036
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.094	469	42.2	0.71733	4	4.77667	6.7	5.2	6.094	468.7	3.6	0.741931
12	6.134	479	43.1	0.342242	2	0.913083	1.3	1	6.134	478.4	1.5	0.342485
13	6.157	485	43.6	0.921568	3	5.77985	8.1	6.3	6.157	485.3	3.5	0.939033
14	6.197	495	44.5	0.112587	2	0.460697	0.6	0.5	6.197	494.1	2.1	0.124495
15	6.28	516	46.4	0.771846	8	9.79276	13.7	10.6	6.28	517.9	8.1	0.683684
16	6.36	536	48.2	0.316031	2	1.05538	1.5	1.1	6.36	536.2	2.2	0.265271
17	6.383	542	48.7	0.298863	2	1.15415	1.6	1.2	6.383	542.1	2.3	0.280076
18	6.415	550	49.5	0.464092	3	2.51552	3.5	2.7	6.415	550.3	3.1	0.454696
19	6.518	576	51.8	0.448874	2	1.5619	2.2	1.7	6.518	575.8	2.1	0.42965
20	6.695	604	54.3	0.21477	2	0.810783	1.1	0.9	6.695	603.5	2.3	0.202269
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.858	627	56.4	0.177163	3	0.781492	1.1	0.8	6.858	627.3	2.6	0.171178
22	6.891	633	56.9	0.125688	2	0.587653	0.8	0.6	6.891	632.2	2.2	0.14849
23	7.081	668	60.1	0.114613	2	0.347745	0.5	0.4	7.081	667.8	1.7	0.116175
24	7.135	678	61	0.614997	2	2.26252	3.2	2.4	7.135	677.8	2	0.623239
25	7.211	692	62.2	0.242063	2	1.02878	1.4	1.1	7.211	691.5	2.4	0.237975
26	7.266	702	63.1	0.0873334	2	0.294289	0.4	0.3	7.266	701.7	1.9	0.0887106
27	7.347	717	64.5	0.245422	3	1.10208	1.5	1.2	7.347	717.3	2.6	0.241157
28	7.395	726	65.3	0.599147	3	3.11004	4.4	3.4	7.395	725.8	2.9	0.599997
29	7.516	749	67.4	0.0401222	3	0.284854	0.4	0.3	7.516	747.3	3.1	0.051887
30	8.852	1006	90.5	0.149038	8	2.14537	3	2.3	8.852	1005.7	7.7	0.157701

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 21
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 23
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	18	1.6	0.31956	8	4.50035	9.7	7.2	3.5	20.2	8.4	0.304064
2	4.55	169	15.2	0.435644	5	3.37164	7.3	5.4	4.55	168.8	4.9	0.391137
3	5.2	253	22.8	0.341948	5	2.84294	6.1	4.6	5.2	252.5	4.8	0.335931
4	5.85	406	36.5	0.854287	3	4.81438	10.4	7.8	5.85	406.1	3.2	0.857203
5	6.55	585	52.7	0.399825	4	2.59564	5.6	4.2	6.55	584.7	3.8	0.385973
6	6.85	626	56.3	0.212682	7	2.34092	5	3.8	6.85	625.3	6.9	0.190566
7	7.35	718	64.6	0.816407	7	9.88504	21.3	15.9	7.35	717.4	7.2	0.774783
8	8.15	868	78.1	0.185497	11	3.74766	8.1	6	8.15	865.8	11	0.192434
9	8.45	928	83.5	0.239614	8	3.02051	6.5	4.9	8.45	927.4	7.7	0.221293
10	8.65	964	86.8	0.235722	6	2.25767	4.9	3.6	8.65	963.7	5.8	0.218465
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1093	98.4	0.849793	5	7.03371	15.2	11.3	9.3	1091.7	4.9	0.816772

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
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PeakCount: 127

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 11.400 4.550 6.400 5.200 6.300 5.850 10.600 6.550 5.200 6.850 5.800 7.350 18.200 8.150 3.900 8.450 4.900 8.650 4.000 9.233 11.700 9.300 11.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 12 Return to Lane Navigator
3.561 16.200 4.173 1.600 4.261 2.400 4.601 1.600 5.120 2.600 5.837 6.200 5.972 8.100 6.005 6.700 6.066 7.400 6.099 1.300 6.123 8.800 6.181 0.600 6.254 2.500 6.340 12.000 6.364 0.400 6.401 2.000 6.503 2.300 6.668 0.900 6.839 0.900 6.957 0.700 7.082 0.500 7.159 3.200 7.202 0.900 7.268 0.600 7.345 1.300 7.401 4.200 7.514 0.400 8.845 3.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 17 LANE TYPE: Specimen ACTUAL GEL LANE: 19 PeakCount: 28 Return to Lane Navigator
3.500 8.800 4.550 8.700 5.200 8.200 5.850 13.100 6.550 7.100 6.850 5.300 7.350 22.200 8.150 7.000 8.450 6.200 8.650 5.000 9.300 8.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 18 LANE TYPE: pI Markers ACTUAL GEL LANE: 20 PeakCount: 11 Return to Lane Navigator
3.500 13.000 4.151 1.400 4.254 1.700 4.550 1.700 4.626 1.900 5.132 5.600 5.200 0.300 5.850 6.800 6.007 5.600 6.043 3.800 6.099 4.100 6.136 0.700 6.160 5.200 6.208 0.300 6.289 9.200 6.381 0.600 6.409 0.700 6.441 1.400 6.550 1.500 6.764 0.500 6.850 0.900 6.921 0.700 7.029 0.500 7.149 0.300 7.230 2.600 7.285 0.900 7.350 4.200 7.423 0.600 7.475 3.100 7.584 0.300 8.150 1.500 8.450 2.000 8.650 6.800 8.858 2.800 9.300 6.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 19 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 21 PeakCount: 35 Return to Lane Navigator
3.507 16.400 4.181 2.600 4.281 1.700 4.653 1.900 5.189 2.400 5.856 1.400 5.884 4.600 5.983 0.700 6.011 5.400 6.046 5.700 6.094 6.700 6.134 1.300 6.157 8.100 6.197 0.600 6.280 13.700 6.360 1.500 6.383 1.600 6.415 3.500 6.518 2.200 6.695 1.100 6.858 1.100 6.891 0.800 7.081 0.500 7.135 3.200 7.211 1.400 7.266 0.400 7.347 1.500 7.395 4.400 7.516 0.400 8.852 3.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 20 LANE TYPE: Specimen ACTUAL GEL LANE: 22 PeakCount: 30 Return to Lane Navigator
3.500 9.700 4.550 7.300 5.200 6.100 5.850 10.400 6.550 5.600 6.850 5.000 7.350 21.300 8.150 8.100 8.450 6.500 8.650 4.900 9.300 15.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 21 LANE TYPE: pI Markers ACTUAL GEL LANE: 23 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Rainbow (Steelhead) Trout ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Rainbow (Steelhead) Trout ---- Go to Display Gel Lane Navigator.