The enhancing effect of dextran sulfate (DS) on delayed hypersensitivity to nonviable Mycoplasma pulmonis in mice was evaluated by means of delayed footpad swelling. Mice pretreated subcutaneously with nonviable M. pulmonis and DS had significantly increased delayed footpad swelling when the mice were injected subcutaneously in the footpad with the same antigen 7 days later. Delayed footpad swelling was not detected in mice pretreated only with nonviable M. pulmonis or DS followed by footpad injection with nonviable M. pulmonis. Subcutaneous pretreatment in the scruff of the neck with nonviable M. pulmonis and DS was more effective in eliciting delayed footpad swelling than intravenous or intraperitoneal pretreatment. Delayed footpad swelling did not develop when mice were injected in the footpad with nonviable Mycoplasma hyopneumoniae, M. gallisepticum, M. dispar, or M. bovis on day 7 after pretreatment with M. pulmonis and DS. Injection of nonviable M. pulmonis in the footpad on day 3 after pretreatment with M. pulmonis and DS resulted in no significant footpad swelling, but injection in the footpad on days 7, 14, 21, and 28 resulted in significant footpad swelling. Delayed footpad swelling was transferred by intravenous injection with spleen cells of mice which were pretreated with nonviable M. pulmonis and DS into nontreated recipient mice. Intravenous injection with anti-mouse thymus cell rabbit serum into the mice which were previously pretreated with nonviable M. pulmonis and DS reduced the delayed footpad swelling significantly.

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