IV.6 The genetic male sterile barley collection.
E. A. Hockett. Western Region ARS, U.S.D.A. and Plant and Soil Science Department, Montana State University, Bozeman, Montana 59715, USA.
The 1974 report contains an error in assigning stock number 346 and msg,,cx to the CI 15514 Hector mutant. The correct designation for the CI 15514 Hector mutant is shown in Table 2. The mutant which had already been assigned msg,,cx is HA 6-33-02 from Finland (Table 2).
The msg,,cw mutant in last year's report (2) had a shortage of male sterile plants when a 3:1 ratio was tested. Additional data from 1974 added to last years results gives a ratio of 80:17 of fertile : sterile plants and a P of .2 - .1 of fitting a 3:1 ratio. The selfed seeds for msg,,cs and msg,,cv shown in last years report were planted at Bozeman in 1974 and all seeds produced sterile plants. This indicated that selfing had occurred in these genotypes. On closer examination, the two seeds listed as selfs for msg,,cw in last year's report, proved to be ergot sclerotia and not selfed seed.
Table 1 gives the data for four mutants found to be allelic with previously designated male sterile mutants.
Table 1. Ratios of fertile to male sterile F1 plants from crosses of genes determined to be allelic.
The segregation ratios and selfing behavior of 15 mutants is shown in Table 2. Actually two of these lines, msg,,dc and msg,,dg, are partial steriles and not male steriles since they have 77.6 and 68.9% selfing respectively. They also do not segregate 3:1 for fertile : sterile plants (Table 2). The mutants msg,,cy, msg,,df, and msg,,dj have too few male sterile plants and msg,,di has too many sterile plants for a good fit to a 3:1 ratio of fertile:sterile. No sib cross data is available at this time to shed further light on these male sterile segregations. The mutant msg,,dl found in Hector is undoubtedly an outcross rather than a sib cross of Hector, since this line is quite variable for plant height and appearance. The data on the mutant msg,,cx in HA6-33-02 in Table 2 is taken from the recent publication by Ahokas (1).
Table 2. Ratios of fertile to sterile plants in F2 and selfing behavior of sterile plants.
Information on linkage relationships of msg24v and msg25r with Bl 1 on chromosome 4 and of msg1Oay with n, lk2 and Bl2 on chromosome 1 are given in (3). Another thesis (4) gives information on linkage between seedling lethals and the following: msg1Oay, msg14cm, msg23b (chr 1); msg2cb (chr 2); msg5ce (chr 3); msg1ca (chr 5); and msg16co (chr 7).
References:
Ahokas, H. A spontaneous male sterile mutant derived from a cultivated X wild barley cross. Ann. Bot. Rennici 11: 25-28.
Hockett, E. A. The genetic male sterile barley collection. Barley Genet. Newsletter 4: 121-123.
Kushnak, G. D. Utilizing linkages of genetic male sterile and aleurone color genes in hybrid barley (Hordeum vulgare L.) systems. Ph.D. Thesis. Mont. State Univ. 123 p. Bozeman, MT.
Rahman, M. M. Balanced male sterile-lethal systems for hybrid barley production. Ph.D. Thesis Mont. State Univ. 93 p. Bozeman, MT.
