Ben KL, Zheng YT; International Conference on AIDS.

Int Conf AIDS. 1998; 12: 782 (abstract no. 41203).

Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnah, P.R. China.

OBJECTIVES: To find new anti-HIV-1 ribosome-inactivating proteins (RIPs) METHODS: The anti-HIV-1 activities of 57 RIPs and crude proteins from 17 species of plants were examined by the following measurement: 1) the inhibition of syncytia formation; 2) the protection of HIV-1 infected cell; 3) inhibition of HIV-1 infected cell fusion in coculture; 4) reduction of p24 antigen expression and numbers of HIV antigen positive cells in acutely and chronically infected culture. RESULTS: Among 57 proteins, trichosanthin (TCS) (SI = 193.3), trichobitacin (SI is between 300-900) from Trichosanthes kirilowii, alpha-momorcharin (alpha-MMC) from Momordica charantia (SI = 332), Protein fractions V (SI = 243) and VI (SI > 1200) from Trichosanthes damiaoshanesis were found to obviously inhibit syncytia formation induced by HIV-1. Crotin I from Croton tiglium, luffin from Luffa cylinarica, RIPs from Hodgsonia macrocarpa and Entada phaseoloides showed slight inhibition of the syncytia formation (SI < 50). Crude protein extracts from Trichosanthes ovigera, Momordica macrophylla and an unnamed plant growing in Xishuangbanna district, Yunnan province, obviously inhibited syncytia formation, but proteins purified from them lost their activities. All RIPs were unable to block HIV infected cell fusion in coculture, and did not protect HIV-1 infected MT-4 or C8166 cells from dying. TCS, alpha-MMC and trichobitacin markedly reduced both expression of p24 core protein and the numbers of HIV antigen positive cells in acutely but not chronically HIV-1 infected culture. CONCLUSION: In addition of TCS and the other known RIPs, trichobitacin is a novel anti-HIV ribosome-inactivating protein. alpha-MMC is a main component of momorcharin with anti-HIV activity.

Publication Types:

• Meeting Abstracts

Keywords:

• Anti-HIV Agents
• China
• HIV
• HIV Core Protein p24
• HIV Envelope Protein gp120
• HIV Integrase
• HIV Integrase Inhibitors
• HIV Protease
• HIV Protease Inhibitors
• HIV-1
• Humans
• MMC protein, Momordica charantia
• Plant Proteins
• Plants
• Ribosomal Proteins
• Ribosomes
• Trichosanthin
• crotin II protein, Croton tiglium
• immunology
• reverse transcriptase, Human immunodeficiency virus 1

Other ID:

• 98402819

UI: 102230851

From Meeting Abstracts