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| Title: |
Gene activity during germination of spores of the fern, Onoclea sensibilis. Cell-free translation analysis of mRNA of spores and the effect of alpha-amanitin on spore germination
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| Author(s): |
Raghavan, V.
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| Abstract: |
Poly(A)-RNA fractions of dormant, dark-imbibed (non-germinating) and photoinduced (germinating) spores of Onoclea sensibilis were poor templates in the rabbit reticulocyte lysate protein synthesizing system, but the translational efficiency of poly(A)RNA was considerably higher than that of unfractionated RNA. Poly(A)RNA isolated from photoinduced spores had a consistently higher translational efficiency than poly(A)RNA from dark-imbibed spores. Analysis of the translation products by one-dimensional polyacrylamide gel electrophoresis showed no qualitative differences in the mRNA populations of dormant, dark-imbibed, and photoinduced spores. However, poly(A)RNA from dark-imbibed spores appeared to encode in vitro fewer detectable polypeptides at a reduced intensity than photoinduced spores. A DNA clone encoding the large subunit of maize ribulose bisphosphate carboxylase hybridized at strong to moderate intensity to RNA isolated from dark-imbibed spores, indicating the absence of mRNA degradation. Although alpha-amanitin did not inhibit the germination of spores, the drug prevented the elongation of the rhizoid and protonemal initial with a concomitant effect on the synthesis of poly(A)RNA. These results are consistent with the view that some form of translational control involving stored mRNA operates during dark-imbibition and photoinduced germination of spores.
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| NASA Center: |
NASA (non Center Specific)
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| Publication Date: |
1992
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| Document Source: |
Other Sources |
| No Digital Version Available: |
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| Document ID: |
20040090166
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| Publication Information: |
Journal of plant physiology, Volume 140, 4, 434-40, ISSN 0176-1617 |
| Contract-Grant-Task Number: |
NAGW-901 |
| Keywords: |
CELLS (BIOLOGY); GENETICS; GERMINATION; RIBONUCLEIC ACIDS; SPORES; TRANSLATING; NASA Discipline Number 29-20; NASA Discipline Plant Biology; NASA Program Space Biology; Non-NASA Center; EXOBIOLOGY; GENES; PLANT PHYSIOLOGY; PLANTS (BOTANY);
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| Accessibility: |
Unclassified; Copyright; Unlimited; Publicly available; |
| Updated/Added to NTRS: |
2006-08-02 |
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