ILcccivctl for pul~licntion 1;cl)ru:iry 4, 195.1

265

(2), onc c:in solvc for tlic conccntr:ition of tlic
cornplcs. Sultstitiitiiig in cqu:itiini (3), wc 1i:rv.c

~vlicrc 1' is thc rntc of cnzymc syntlicsis at
inducer sntur:ition for fisccl IT] and is crpnl to
Ii'[r]. K. is tlic dissociation constant of thc Ti
coniplry. Itcl:ition (4) is foriii:illy iclcnticnl to thc
X~licli:it~lis-~~Icnteii crlri:ttioii. It cannot, Iiowcrer,
lie cmployctl :IS hiith siiicc in .our rsi)criincntaI

sptciii   is riot corist:iiit with tinw. Sincc the

intlurcr ciiiploycil is riot iiict:holizwl, [i] is con-
st:int in :my givcii csl)rrin~rnt. Cloiiscriiicntlj-, 1'
 nil, tlicrcfow, [B] inust hc furlrtionx of tinic. TIN
n:ittirc of tlic functian to lit: spccilictl is siiggcsted
by Llic olwrv:itioii t1i:it tlic kinctics of cnzyiie
syiitliosis in yc:ist untlcr tlic csptviiiiciitnl
cowlitions cmploycil Iterc povcss :in csponcntinl
pli:isc. \Yc :iswiiie, thci cforv, t1i:it B vwics cs-
poiieiiti:illy with tim :iiiiI inrrr:iscs in tlic forni
of thr, ai winp!cs. `l'tic I:dtvr t:hs :ircoiiiit of
tlic f:wt th:it iiirrr:iv iii rrizytiic for riiiiifi r:ip:icity
docs iiot take pl:u.c ii~il~ss iiitluccr in picscnt.
Wc fintl tlirri tli:it

dls'
Ill

~ YucW'li]\l~li Kelt (5)

\Vlicrc I" is tlic "Krowtli constant" of r nt
sntiir:iting iiitliiccr conccntr:\tioiis, BO tlic value of
 it time zero, :uid fi. is tlic tlissoci:ttion constant
of tlic ;ri-coniplcs.
Fiil)stitutiiix (5) in (q), iiitcgr:tting and
cv:ilir:ding tlic resulting corist:int yicklsL in

8-


6-

nf the inducer is, froiii cqirntiori (7), fcmii:illy
it1cntic:d to t he Xlic,li:~cli~-hIriit~,i~ rd:itioiisliip.
Tlic 1rsri:i1 nirtliotls tii:iy lic cniplty.wl. tlimfnrc,
on tlic rcsitlting t1:it:i to tlctcriiiiric tlicr I<, \dit? of
a-mcthyl filucosicI(* :IS :in iniliiccr. Sliorild T
turn out to 1)c tlic nizynic itself, tlrc f<, so.-
dctcrniinctl shoiiltl coincide with t lint ol)t:iinetl in
espcrinicnts Iiio:isuririg coml)iiintion I>ctrvccn
a-methyl glticosidc :mtl tlic criaytiic.
Rc mny stiniin:irix Iyc, I)ricfly, the tletnils
of Iiow siich cul)criiiirnts were wriird out.
I~spoticnti:il 1)Ii:isc cells wrc hnrvestctl :tiid
wnshccl ininic(1i:itcly prior tlJ tlicir use. Tlicii
thcy `sere siispcntlctl in nietliiim 13 to n tlcnsity of
2.5`1 ~ig tlry wiglit CJr cclls per nil. Iridiictions
wrrc condiirtctl :it :i st!rios of a-nictliyl gliicosidc
conccntrations in :icr:itrtl tirl)cs ;)lncwl in 11 wtcr
bath liclrl :it 30 C. Air c.nct~y hourcc wis piovidctl
in thc form of gliicosc :it :L cwiccntr:ttion of
3.3 nig pr nil. It h:d I)crn slirnvii in prcviniir
espcrinirnts (Siiic~jiclru:in rind I I:ilvormi, 1 !GI)
that this Irvcl tlocs not interfore with tlic onsrt or
suhcquc`nl pioptw of in:tlt:iw \yiitlic4s.
FoliowiIig tlic :itltlition of the itiditccr, s:rniplcs
acrc reixivcd :it intcrwls, :iiitl tlicir ciizyrnc
content st:iliilizrtl with rr1tr:rviolt~t liglit :IS
dexcrilwd tinder AIctIiw{s. `I'hc cclls tlicii wrc
aaslwl witti rliillwl wtw, I cwqvmkxl in 0.1 lr
plioxpli:itc-sric,t.in:itc bdfcr :it 1111 4.5, nncl tlicir
cnrymc :ictivity :is:iycd iii:inoiiictfic,:illy ovcr :L
period of c;n niinritcs. At c:icli a-tncthyl gliicositlc
conccntr:itioii htcd :i niininmii of tlircc s:iniplcs
was tnkrri in the (q)onmti:il period of cnzynic
form:ition for tlic tlcteriniii:ttioii of rate of cnaymc

SJntlrrSis. It \v:1s f~illnd t1i:it :It tllC low lovcls of

0.151
n .O.Z
n.010
0. I!` Iz'
0.1101

14.0 '""i. /

0 40. 80 120 IC0 200

I/S

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