Dates:

18 May 2000 depart Dutch Harbor, AK
30 May 2000 arrive Dutch Harbor, AK

Edward D. Cokelet, Ph D
NOAA/PMEL
7600 Sand Point Way NE
Seattle, WA 98115-6439
Phone: (206) 526-6820
Fax: (206) 526-6485
E-mail: cokelet@pmel.noaa.gov

1.1 Program Description:

The Fisheries Oceanography Coordinated Investigations (FOCI) are an effort by NOAA scientists to understand the physical and biological processes that determine recruitment variability of commercially valuable fin-fish and shellfish stocks in Alaskan waters. FOCI consists of several projects including the present one funded by the North Pacific Marine Research (NPMR) Program.

This project is a collaborative effort by NOAA and academic scientists to understand the influence of mesoscale eddies on continental shelf-slope exchange in the Southeastern Bering Sea. The objectives are to

(1) Detect movements of nutrient-rich slope water onto the shelf and relate them to temporal and spatial variations in chlorophyll,

(2) Identify physical mechanisms that create slope-water fluxes onto the continental shelf,

(3) Detect ocean-color variability in relation to physical processes,

(4) Use shipboard measurements of near-surface optical and biological parameters to validate and extend bio-optical algorithms for use in autonomous sampling and remote sensing, and

(5) Investigate the effects of on-shelf flow on phytoplankton biology.

These instructions in conjunction with the "FOCI Standard Operating Instructions for NOAA Ship Ronald H. Brown, 2000" provide complete information for this cruise. The Chief Scientist is authorized to alter the scientific portion of this cruise plan with the concurrence of the Commanding Officer, provided that the proposed changes will not

(1) Jeopardize the safety of personnel or the ship,

(2) Exceed the time allotted for the cruise,

(3) Result in undue additional expense, or

(4) Change the general intent of the cruise.

Southeastern Bering Sea, along the continental shelf-break, probably no farther north than the Pribilof Islands.

NOAA/Pacific Marine Environmental Laboratory (PMEL)
7600 Sand Point Way NE
Seattle, WA 98115-6439

University of Alaska-Fairbanks (UAF)
School of Fisheries & Ocean Sciences
P.O. Box 757220
Fairbanks, AK 99775-7220

Dalhousie University (Dal)
Department of Oceanography
Halifax, NS B3H 4J1
Canada

 

	Name	Title	Affil.	Sex	Nation
1.	Edward (Ned) Cokelet, Ph D	Chief Scientist	PMEL	M	USA
2.	Carol DeWitt	Field Ops. Spec.	PMEL	F	USA
3.	Elizabeth Dobbins	Research Scientist	PMEL	F	USA
4.	John Cullen, Ph D	Professor	Dal	M	USA
5.	Catherine Brown	Graduate Student	Dal	F	Canada
6.	Richard Davis	Research Assistant	Dal	M	USA
7.	Margaret Davey, Ph D	Post Doctoral Sci.	Dal	F	Canada
8.	Yannick Huot	Graduate Student	Dal	M	Canada
9.	Jason Fox	Graduate Student	Dal	M	Canada
10.	Kristian J. Curran	Graduate Student	Dal	M	Canada
11.	Terry Whitledge, Ph D	Professor	UAF	M	USA
12.	Taekeun Rho	Graduate Student	UAF	M	Korea
13.	Heloise Chenelot	Graduate Student	UAF	F	France

1.7 Administrative

Ship Operations:

CDR Jon Rix, Chief
Chief, Operations Division
Marine Operations Center, Atlantic
439 West York St.
Norfolk VA 23510
Phone: (757) 441-6842
Fax: (757) 441-6495
E-mail: Jon.E.Rix@noaa.gov

LCDR Jim Meigs
Marine Operations Center, Atlantic
439 West York St.
Norfolk VA 23510
Phone: (757) 441-6844
Fax: (757) 441-6495
E-mail: Jim.Meigs@noaa.gov

LCDR Dave Mattens
Pacific Marine Environmental Laboratory
7600 Sand Point Way NE
Seattle WA 98115-6439
Phone: (206) 526-4485
Fax: (206) 526-6815
E-mail: mattens@pmel.noaa.gov

2.1 Data to be collected:

Measurements will be collected with shipboard sensors including the ADCP and Sea Beam, a CTD profiler with water bottles, and user-supplied mast-mounted radiometers, tethered spectral radiometer buoy (TSRB) and spectral radiometer profiler. A few satellite-tracked drifting buoys will also be launched.

2.1.1: SCS will be configured to record the following:

2.2.3: Sea Beam data may be collected on site-specific surveys as required and stored for transport back to PMEL.

2.2.4: CTD cast data will be collected on the ship’s Sea Bird 911+ system. There will be approximately 100 CTD casts. 12 10-liter water samples will be taken on most casts.

2.2.5: AutoSal runs will be done to compare salinities with CTD values. Usually 2 samples will be taken per cast - one at depth and one in the surface mixed layer. This could be relaxed to one sample per cast - alternating between deep and mixed-layer samples.

2.2.6: AVHRR and SeaWiFS satellite observations of SST and ocean color will be downloaded using TeraScan and full-resolution, geo-referenced images made available in a timely manner to facilitate cruise planning. In clear weather these should prove invaluable for locating nutrient-rich slope water that moves onto the shelf and promotes phytoplankton blooms.

2.2.7: The ship will maintain a Marine Operations Abstract (MOA) on paper giving the date, time and location of significant events such as CTD casts, buoy deployments, etc.

2.2 Staging plan:

Gear not already aboard the vessel will be shipped to Dutch Harbor and loaded on 17 May 2000. Access to the ship as soon as possible after its arrival in port is needed because the chemical analysis equipment requires extensive set-up. The scientific party has secured use of an NMFS van to move gear from the airport and/or the docks to the ship in Dutch Harbor.

The goal of the cruise is to locate one or more eddies interacting with the Southeastern Bering Sea shelfbreak and sample the evolving flux of nutrient-rich slope water onto the continental shelf and associated primary production changes. Before and during the cruise, eddies will be located in three ways:

2.3.2: The speed and direction of the drifters within the onshore flow will not tell us its width, but previous work indicates that the breadth of an episodic onshore flow event is approximately 10-20 km. Estimates of jet size from remote sensing and onboard analysis of data will allow for real-time modification of the sampling scheme to accommodate the actual size of the jet encountered, within the available ship time. The ship will follow a zig zag pattern, crossing the onshelf flow approximately 5 times until the 100 m isobath is reached. Stations will be occupied every 5 to 10 km along any one diagonal.

2.3.3 Alternative Plan:

Drifters deployed on an earlier cruise along with altimeter data will be used to identify possible sites of episodic onshelf flow. There is, however, the chance that no onshelf flow events will be identified. If an episodic event is not located using either method, then the presence and effects of the relatively persistent onshelf flow, as evident in drifter trajectories and hydrographic surveys from past years, at either Bering Canyon or Pribilof Canyon will be examined. The sampling schedule will be as described, proceeding from the head of the canyon towards the shelf.

The Chief Scientist will e-mail the approximate location of the study area to the ship a few days in advance, once we have determined it from satellite-tracked drifting buoy trajectories and satellite images. Owing to the dynamic nature of the interaction of Bering Slope Current eddies with the shelf break, it will not be possible to provide sampling points until the day of departure. The sampling area will evolve with the eddy interaction. Figure 1 shows the eddy-shelf-interaction study area (box) and Mooring 2 and 3 sites.

CTD operations will proceed 24 hours per day in the study area for ~100 casts. We request that the ship provide 2 Survey Technicians to support these casts and ~200 AutoSal salinity determinations. The CTD will be deployed with 12 10-liter bottles on its rosette, a flash fluorometer, a beam transmissometer and an altimeter.

Several underway measurements are required. The Thermosalinograph, flow-through fluorometer, and ADCP will be used continuously. These and other SCS data should be logged throughout the cruise.

2.6.2: Sea Beam - Bering Sea eddies may interact with the continental shelf in submarine canyons and at discontinuities in the shelf break. Since Bering Sea bathymetry is poorly known, we may wish to map the bottom using Sea Beam in areas of eddy impingement.

2.7 Applicable restrictions:

None.

None planned.

Gear will be off-loaded in Dutch Harbor on 30 May 2000 and transported via NMFS van to the airport.

3.1 Equipment and capabilities provided by ship:

Oceanographic winch with slip rings and 3-conductor cable terminated for CTD,

Readout for oceanographic winch,

Sea Bird 911 plus CTD system including underwater CTD with twin temperature and conductivity sensors (plus spares), 12-bottle rosette, pinger, weights, deck unit, PC with Seasoft software and tape recorder,

10-liter sampling bottles for use with rosette (12 plus spares),

AUTOSAL salinometer for CTD salinity calibration,

Thermosalinograph,

Flow-through fluorometer,

For meteorological observations: radiometer, 2 anemometers, barometer, air temperature sensor, relative humidity sensor and rainfall sensor,

Freezer space for storage of biological and chemical samples (-20 F or colder, at least 10 cu. ft.),

Bench space (at least 10 linear feet for productivity experiment filtering),

Salt water outlets to cool productivity deck incubator(s) in an unshaded, but protected, deck area,

Laboratory refrigerator (at least 6 cu. ft.),

Distilled or reverse-osmosis water source,

Laboratory space with exhaust hood, sink, lab tables and storage space,

Echo sounders for deep and shallow water measurements,

RDI 150-KHz ADCP with position input from P-code GPS receiver, heading input from Ring-Laser Gyro and Seapath 200 and output to Iomega Zip drive,

SCS (Shipboard Computer System),

One or more networked PCs,

Network connection for science-party-supplied Macintosh, NT and Unix computers,

Networked black-and-white and color PostScript printers,

Internet access for downloading TOPEX/Poseidon sea-surface height anomaly plots and SeaWiFs images,

TeraScan oceanographic data satellite downlink and image analysis system,

Sea Beam 2112 swath bathymetric sonar system,

Adequate deck lighting for night-time operations,

Safety harnesses for working on deck,

Float coats.

PMEL:

• Profiling spectroradiometer

• Floating spectroradiometer

• 2 mast-mounted spectroradiometers

• Profiling FRR fluorometer

• Profiling ac-9 absorption/attenuation meter

• Profiling Hydroscat backscatter meter

• Benchtop fluorometer

• Filter rigs

• Various computers

• Beam transmissometer for CTD

4.1 Data responsibilities:

The Chief Scientist will receive all original data gathered by the ship for the primary project, and this data transfer will be documented on NOAA Form 61-29 "Letter Transmitting Data". The Chief Scientist in turn will furnish the ship a complete inventory listing all data gathered by the scientific party detailing types and quantities of data. The Chief Scientist will be responsible for the disposition, feedback on data quality, and archiving of data and specimens collected on board the ship for the primary project. The Chief Scientist will also be responsible for the dissemination of copies of these data to participants in the cruise, to any other requesters, and to notify NODC of measurements and samples taken at sea via a Cruise Summary Report (IOC ROSCOP, Third Edition). The ship may assist in copying data and reports insofar as facilities allow. Metadata describing data collected during FOCI, SEBSCC, and NPMR cruises must be submitted to the Bering Sea Ecosystem Biophysical Metadatabase within one month of completion of the cruise. On-line guidance and submission forms are available through the World Wide Web at http://www.pmel.noaa.gov/bering/mdb/. Alternatively, forms may be requested from the FOCI Coordinator.

4.1.2: Individuals in charge of supplementary ("piggyback") projects conducted during the cruise have the same responsibilities for their project's data as the Chief Scientist has for primary project data. All requests for data should be made through the Chief Scientist.

4.1.3: The Commanding Officer is responsible for all data collected for fleet ancillary projects until those data have been transferred to the project's principal investigators or their designees. Data transfers will be documented on NOAA Form 61-29. Copies of fleet ancillary project data will be provided to the Chief Scientist when requested. Reporting and sending copies of fleet ancillary project data to NESDIS (ROSCOP) is the responsibility of the program office sponsoring those projects.

4.2 Pre- and post-cruise meetings:

All scientific personnel will meet with ship’s representatives in a pre-cruise meeting the day of departure to discuss scientific objectives, operations, safety and Standing Orders. A post-cruise meeting will be scheduled between the Chief Scientist and Commanding Officer for a convenient time at the end of the cruise. Project accomplishments will be reviewed, as will general aspects of ship's performance and any administrative issues.

Reporting requirements for the Shipboard Operations Evaluation Form, to be completed and submitted by the Chief Scientist to the Office of Marine and Aviation Operations (OMAO) within 30 days of cruise completion, will also be reviewed.

Any additional work will be subordinate to the primary project and will be accomplished only with the concurrence of the Commanding Officer and the Chief Scientist(s).

5.1 Supplementary ("Piggyback") projects:

A. Underway Measurements in support of Global Carbon Cycle Research (GCC)

A1. Request:

As part of the ongoing research to quantify the CO2 uptake by the world's oceans we have installed underway systems on the BROWN. On many cruises we request bunk space for one scientist of our laboratories to maintain the many systems outlined below. If we cannot send a dedicated person we try to have a scientist of the specific scientific party look after the Underway pCO2 system (described in section A4 below). On some cruises we are unsuccessful in attracting a volunteer and would like to use the services of the survey technician for the Underway pCO2 system only . After initial start-up, which requires about one hour of monitoring, the system needs checking twice a day requiring a total of about 20-minutes. We would also request weekly data downloads and transmission such that we can perform on shore near-real-time quality control to assess if the instrument is operating satisfactorily. All costs of the email transmissions and survey technician overtime would be covered by AOML. The chief survey technician, J. Shannahoff, has operated the instrument before with good results. In the event of system malfunction that cannot be easily repaired, we will ask Mr. Shannahoff to shut the system down. The shoreside leader of the effort, Mr. Robert Castle has interacted closely with J. Shannahoff and feels that this arrangement would work well.

The underway sensors on RHB will be used in support of the objectives of the Global Carbon Cycle Research (GCC) to quantify the uptake of carbon by the world's ocean and to understand the bio-geochemical mechanisms responsible for variations of partial pressure of CO2 in surface water (pCO2). This work is a collaborative effort between the CO2 groups at AOML and PMEL.

Principal investigators:

Dr Rik Wanninkhof 305-361-4379 wanninkhof@aoml.noaa.gov AOML

Dr. Richard Feely 206-526-6214 feely@pmel.noaa.gov PMEL

The semi-automated instruments are installed on a permanent basis in the hydrolab of RHB and are operated by personnel from AOML and PMEL. All work is performed on a not-to-interfere basis and does not introduce any added ship logistic requirements other than the continuous operation of the bow water pump and thermosalinograph. This effort requires one permanent berth for the operator of the systems. The instrumentation is comprised of an underway system to measure pCO2, a SOMMA (single operator multi-parameter metabolic analyzer)-coulometer system to measure total dissolved inorganic carbon, a Turner Designs fluorometer, and a YSI oxygen probe. An oxygen titrator and stand-alone fluorometer will be used to calibrate the underway oxygen and fluorometer, respectively. All the instruments are set up along the port side wall and aft bench in the hydrolab.

Current estimates of anthropogenic CO2 uptake by the oceans range from 1 to 2.8 Gigatons per year. The CO2 fluxes between air and water are poorly constrained because of lack of seasonal and geographic coverage of delta pCO2 (the air-water disequilibrium) values and incomplete understanding of factors controlling the air-sea exchange of carbon dioxide. Seasonal and temporal coverage can be increased dramatically by deploying pCO2 analyzers on ships.

The effort on the RHB is expanded beyond the historical scope of the underway programs by incorporating additional sensors to improve our understanding of the factors controlling pCO2 levels.

A. Underway pCO2 system

This system consists of a large (40-liter) air-water equilibrator requiring an unobstructed drain at floor level for the 15 L/min outflow, an infrared analyzer with valves and flow meters, and a computer controlling the operating sequence and which also logs the data. The underway pCO2 system is an integrated package for measurement of pCO2 in air and water and support sensors necessary to reduce the data (such as equilibrator temperature, location, salinity, sea surface temperature and barometric pressure). This system is an upgrade from the initial systems and requires routine checks at 6-12 hour intervals, including logging of mercury thermometers in the equilibrator.

This is a compact pulsed electrode unit which also contains a temperature sensor. This is a new sensor built by Dr. Langdon at LDEO. Water requirement is 2-Liter/minute with a bench top drain. One foot of bench space is required. During this cruise the data will be validated against samples taken four time a day and analyzed by potentiometric winkler titrations

This instrument, which was jointly purchased by AOML and AMC for the BALDRIGE, requires a water throughput of about 5 L/min. Periodic cleaning of the flow through cell (2-14 days) is required . The signal of the fluorometer is logged on the shipboard SCS system or on the computer logging the underway pCO2 data. Aliquots of seawater are extracted twice per day and analyzed for chlorophyll and phaopigments on a separate fluorometer following routine procedures to calibrate the fluorometer signal. This information will be particularly useful to extrapolate the observations from the NASA SEAWIFS satellite to in situ pigment concentrations.

1. Continuous seawater supply: 20 lpm minimum, 40 lpm maximum for instruments, and 75 lpm throughput to assure short residence time of water in line and minimal heating.

2. Access to TSG and SCS data: Temperature at intake, salinity from TSG, fluorometer signal, wind speed (true and relative), wind direction (true and relative), time, latitude, longitude, and ship speed.

3. Bench space, hydrolab space, access to bow water line and drains.

Specific questions should be directed to:

Robert Castle, phone 305-361-4418, castle@aoml.noaa.gov

Ancillary tasks will be accomplished in accordance with the NOAA Fleet Standing Ancillary Instructions.

6.1 Policy/Compliance:

Ronald H. Brown will operate in full compliance with all NOAA hazardous materials (HAZMAT) requirements. All hazardous materials and substances needed to carry out the objectives of the embarked science mission, including ancillary tasks, are the direct responsibility of the embarked designated Chief Scientist, whether or not that Chief Scientist is using them directly. The ship's Environmental Compliance Officer will work with the Chief Scientist to ensure that this management policy is properly executed.

6.1.2: The Chief Scientist will provide the Commanding Officer with an inventory indicating the amount of each hazardous material brought onboard, and for which the Chief Scientist is responsible. This inventory shall be updated at departure from the ship, accounting for the amount of material being removed, as well as the amount consumed in science operations and the amount being removed in the form of waste.

6.1.3: The ship's dedicated HAZMAT Locker contains two 45-gallon capacity flame cabinets and one 22-gallon capacity flame cabinet, plus some available storage on deck. All HAZMAT, except small amounts for ready use, must be stored in the HAZMAT Locker. If science party requirements exceed ship's storage capacity, excess HAZMAT must be stored in dedicated lockers meeting OSHA/NFPA standards to be provided by the science party.

6.1.4: The scientific party, under supervision of the Chief Scientist, shall be prepared to respond fully to emergencies involving spills of any mission HAZMAT. This includes providing properly trained personnel for response, as well as the necessary neutralizing chemicals and clean-up materials. Ship's personnel are not first responders and will act in a support role in the event of a spill. The Chief Scientist shall provide a list of science party members that are properly trained to respond in the event of HAZMAT spills.

6.1.5: The Chief Scientist is directly responsible for the handling, both administrative and physical, of all scientific party hazardous wastes. No liquid wastes shall be introduced into the ship's drainage system. No solid waste material shall be placed in the ship's garbage.

6.1.6: The embarking Chief Scientist will work with the departing Chief Scientist and the ship's Environmental Compliance Officer to ensure proper tracking of inherited hazardous materials.

6.2 Inventory:

PMEL: None

UAF: See Section 9.3.1.1

Dalhousie: See Section 9.3.2.1

UAF: MSDS Forms are packed with chemicals and will transferred from the Miller Freeman when the chemicals are unloaded. A spare copy of the MSDS forms will accompany the PI/student.

Dalhousie: See Section 9.3.2.2.

7.1 Radioactive isotope policy:

Ronald H. Brown has no specially designed lab space for working with isotopes. We will therefore require that all radioisotope work be done in a dedicated van with its own storage area and separate waste discharge. This policy is consistent with that of the UNOLS fleet. All of the waste should remain segregated from the ship's waste and be packed out by the investigator.

7.1.1: Each scientist working with these materials will be required to wear a lab coat and disposable booties to reduce the likelihood of tracking the substance out of the van and into the ship.

7.1.2: It will be the responsibility of the investigator to conduct pre-cruise (for background) and post-cruise wipe tests (regardless of whether a spill occurred or not). Wipe tests should also be conducted in the event of a spill, as well as periodically while underway.

7.1.3: A detailed procedural methodology describing the use of these materials should be provided to the Environmental Compliance Officer(ECO) for review at least one month prior to bringing them aboard. A spill contingency plan should also be provided at the same time. Please note that ship's personnel are not first responders in the event of a spill.

7.1.4: A log detailing the type and amount of materials brought aboard and removed from of the ship shall be maintained, along with a record of any spills that occurred.

7.1.5: All radioisotope work will be conducted by NRC or State licensed investigators only, and copies of these licenses shall be provided to the ECO at least one month prior to bringing any materials on board.

7.2 Inventory:

PMEL: None

Dalhousie: None

UAF: None

Not applicable.

8.1 Scientific Berthing:

The Chief Scientist is responsible for assigning berthing for the scientific party within the spaces approved as dedicated scientific berthing. The Ops Officer will send stateroom diagrams to the Chief Scientist showing authorized berthing spaces. The Chief Scientist is responsible for returning the scientific berthing spaces in the condition in which they were received; for stripping bedding and for linen return; and for the return of any room keys which were issued.

8.1.2: In accordance with NC Instruction 5255.0, Controlled Substances Aboard NOAA Vessels, dated 06 August 1985, all persons boarding NOAA vessels give implied consent to conform with all safety and security policies and regulations which are administered by the Commanding Officer. All spaces and equipment on the vessel are subject to inspection or search at any time.

8.2 Medical Forms:

The NOAA Health Services Questionnaire must be completed in advance by each participating scientist. It will be sent out by the Chief Scientist and should be returned to him in a separate, sealed envelope marked with the participant’s name, cruise number and cruise dates. It should reach the ship no later than 4 weeks prior to the cruise to allow time to medically clear the individual, to request more information if needed, and to prepare for special circumstances. All personnel must bring with them prescription and routine, over-the-counter medication (e.g. an aspirin a day). Supplies on board are limited, and chances to restock are few.

Prior to departure, the Chief Scientist must provide a listing of emergency contacts to the Executive Officer for all members of the scientific party, with the following information: scientist’s name, emergency contact’s name, address, relationship to scientist, telephone number and e-mail address (if available).

Wearing open-toed footwear (such as sandals) outside of private berthing areas is unsafe and is not permitted. Steel-toed shoes are required to participate in any work dealing with suspended loads, including CTD deployment and recovery, on outside weather decks. All members of the scientific party are expected to be aware of these regulations and to comply with them.

The Chief Scientist or designated representative will have access to ship's telecommunications systems on a cost-reimbursable basis. Where possible, it is requested that direct payment (e.g. by credit card) be used as opposed to after-the-fact reimbursement. Specific information on how to contact the Ronald H. Brown and all other fleet vessels can be found at http://www.pmc.noaa.gov/phone.htm.

Standing Order 9.21-1: In recent years the proliferation of electronic mail (e-mail) and the reduction of INMARSAT costs have permitted the sending of nominal amounts of personal e-mail when transmitted with official ship's business. The availability of e-mail services is a valuable quality of life issue aboard ship. Accordingly, a complimentary amount of personal use will be permitted for all personnel aboard.

8.4.1.2: It should be understood that the cost of personal e-mail being transmitted from shore to an individual aboard ship will be charged against that individual's complimentary amount. A detailed billing statement will be issued periodically to any individual or Chief Scientist whose costs have exceeded his or his group's monthly entitlement. All costs in excess of an individual's or group's complimentary amount must be reimbursed. When personal use cannot be easily distinguished from official business, the amount of reimbursement will equal the total cost minus the complimentary amount. Each embarked personnel will have an e-mail account/address established in his/her name by the Lead Electronic Technician (LET) at the time of arrival. The general format is:

Firstname_Lastname%BROWN@ccmail.rdc.noaa.gov

8.4.2 Satellite Communications:

Standing Order 9.21-2: INMARSAT-A (voice and fax) and INMARSAT-M (voice) communications are available aboard ship and may be used for personal or business related calls so long as the caller makes arrangements to pay for the calls. Credit card calls are the preferred method of payment. INMARSAT calls can be extremely expensive and the exact cost may not be known until you receive your bill. Brevity is encouraged. See the Lead Electronic Technician (LET) for any questions regarding the use of these phones. If you do not have a credit card and need to make an INMARSAT call, arrangements to pay by personal check may be arranged with the XO.

Standing Order 9.21-3: Routine incoming non-emergency phone calls are discouraged. Use e-mail communications for this purpose. In an emergency, embarked personnel can be contacted by phone. Phone numbers for the Ronald H. Brown can be found at http://www.pmc.noaa.gov/phone.htm#RB.

For high speed data transmission, including FTP, and high quality voice telephone communications. Costs range from $5-$11 per minute for use of the service, and may be charged to credit card or otherwise reimbursed.

For voice telephone communications and 2400 baud data transfer. Cost is about $3 per minute to the US and may be charged to credit card, collect or otherwise reimbursed. Mini-M coverage is by spot beam and may not be available in all the areas the ship may be working in.

Messages can also be left with the Marine Operations Center, Norfolk, Virginia, by calling (757)441-6206. After hours and on weekends and holidays, an answering service will relay a message to the appropriate duty officer.

Standing Order 9.22: Incoming letters and packages can be sent to embarked members of the ship's operating crew and scientific complement by addressing them to:

Name
NOAA Ship RONALD H. BROWN
Atlantic Marine Center
439 West York Street
Norfolk, Virginia 23510

Mail received at the marine center will be periodically forwarded to the ship's next port of call. When the ship is on a foreign deployment, senders are encouraged to mail letters and packages earlier to ensure delivery. Be advised that some foreign customs authorities routinely open and inspect incoming mail. Arrangements for ship's outgoing mail will be made on the morning of departure. In foreign ports, mail must have US postage affixed as it will be boxed and overnight-expressed to the Atlantic Marine Center where it will enter the US postal system. US postage stamps are not routinely available aboard ship.

Contractual agreements exist between the port agents and the commanding officer for services provided to the NOAA Ship Ronald H. Brown. The costs or required reimbursements for any services arranged through the ship's agents by the scientific program, which are considered to be outside the scope of the agent/ship support agreement, will be the responsibility of that program. Where possible, it is requested that direct payment be arranged between the science party and port agent, as opposed to after-the-fact reimbursement to the ship's accounts.

Chief Scientist shall be cognizant of the reduced capability of the Ronald H. Brown's operating crew to support 24-hour mission activities with a high tempo of deck operations at all hours. Wage marine employees are subject to negotiated work rules contained in the applicable collective bargaining agreement. Dayworkers' hours of duty are a continuous eight-hour period, beginning no earlier than 0600 and ending no later than 1800. It is not permissible to separate such an employee's workday into several short work periods with interspersed non-work periods. Dayworkers called out to work between the hours of 0000 and 0600 are entitled to a rest period of one hour for each such hour worked. Such rest periods begin at 0800 and will result in no dayworkers being available to support science operations until the rest period has been observed. All wage marine employees are supervised and assigned work only by the Commanding Officer or designee. The Chief Scientist and the Commanding Officer shall consult regularly to ensure that the shipboard resources available to support the embarked mission are utilized safely, efficiently and with due economy.

Equipment Inventory Page 17
Figures Page 18
Hazardous Materials
Hazardous Material Summary- UAF Page 19
Hazardous Materials Summary - Dalhousie University Page 24

PMEL

9.3 Hazardous Materials

9.3.1 Hazardous Material Summary - UAF

9.3.1.1 Chemical Inventory - UAF

ALPKEM Nutrient Analyzer Chemical Inventory

R/V Ron Brown - April 2000

SOLIDS

chemical grams/pack #packs Total
******************************************************************************

Orthophosphate

Ammonium Molybdate 6.0 g 4 24 g

Potassium Antimony Tartrate 3.0 g 4 12 g

Sodium Lauryl Sulfate 15 g 4 60g

Ascorbic Acid 6 g 4 24 g

Potassium Phosphate standard 0.34 g 4 1.36 g

******************************************************************************

Dissolved Silicon (Silicate)

Ammonium Molybdate 10.8g 12 130 g

Tartaric Acid 100 g 8 800 g

Stannous Chloride 40 g 4 160 g

Sodium Silicofluoride standard 1.88 g 4 7.52 g

******************************************************************************

Nitrate plus Nitrite; Nitrite

Imidazole 6.8 g 8 54.4 g

Copper sulfate 2.5 g 4 10g

Copper sulfate 20 g 4 80 g

sulfanilamide 10 g 8 80 g

N-(1-Naphthyl)ethylenediamine- 1 g 8 8 g

Dihydrochloride

Cadmium metal tubing 20 g 3 60 g

Potassium nitrate standard 1.01 g 4 4.04 g

Sodium nitrite standard 0.17 g 4 .68 g

Compressed nitrogen gas 5 ml/min 30 ft3

******************************************************************************

Ammonium

Phenol 35 g 6 210 g

Sodium Nitroprusside Dihydrate 0.4 g 6 2.4 g

Sodium Citrate 140 g 12 1680 g

Sodium Hydroxide 2.5 g 12 30 g

Ammonium Chloride standard 0.14 g 4 0.56 g

******************************************************************************

Chimical Vol/each Total Vol
LIQUIDS

Sulfuric Acid (concentrated) 500 ml 1 liter

Acetone 500 ml 1 liter

Hydrochloric Acid (concentrated) 500 ml 2 liters

Brij-35 50 ml 100 ml

Chlorox (~5% solution) 250 ml 250 ml
 

Chemical Inventory for Shipping

(All Solids, No liquids)

CAS

No. g/pack #packs Total

*****************************************************************************

13106-76-8 Ammonium Molybdate 6.0 g 4 24 g

28300-74-5 Potassium Antimony Tartrate 3.0 g 4 12 g

151-21-3 Sodium Lauryl Sulfate 15 g 4 60g

50-81-7 Ascorbic Acid 6 g 4 24 g

7778-77-0 Potassium Phosphate 0.34 g 4 1.36g

13106-76-8 Ammonium Molybdate 10.8g 12 130 g

00087-69-4 Tartaric Acid 100 g 8 800 g

10025-69-1 Stannous Chloride 40 g 4 160 g

16893-85-9 Sodium Silicofluoride 1.88 g 4 7.52g

288-32-4 Imidazole 6.8 g 8 55 g

7758-98-7 Cupric sulfate 2.5 g 4 10g

7758-98-7 Cupric sulfate 20 g 1 20 g

63-74-1 Sulfanilamide 10 g 8 80 g

1465-25-4 N-(1-Naphthyl)ethylenedi- 1 g 8 8 g

amine Dihydrochloride

7440-43-9 Cadmium metal tubing 3 each

7757-79-1 Potassium nitrate 1.01 g 4 4.04g

7632-00-0 Sodium nitrite .17 g 4 .68 g

108-95-2 Phenol 35 g 6 210 g

13755-38-9 Sodium Nitroprusside 0.4 g 6 2.4 g

6132-04-3 Sodium Citrate 140 g 12 1680g

1310-73-2 Sodium Hydroxide 2.5 g 12 30 g

12125-0209 Ammonium Chloride .14g 4 .56g

Acids: hydrochloric acid, sulfuric acid

*** baking soda (sodium bicarbonate) is provided for neutralization.

*** vermiculite and kitty litter are provided for absorbency material.

Bases: sodium hydroxide

*** dilute hydrochloric acid (10%) is provided for neutralization.

*** vermiculite and kitty litter are provided for absorbency material.

9.3.1.4 Unused and waste chemicals will be offloaded in Dutch Harbor. No chemicals will remain onboard after the cruise.

9.3.1.5 All field party members will be briefed at a pre-cruise meeting on the location and quantity of hazardous chemicals. In addition, cleanup of chemical spills and use of neutralizing agents will be explained. A set of written instructions of cleanup procedures will be posted by the chemical analysis work area.

9.3.1.6 Material Safety Data Sheets (MSDS) - UAF

MSDS Forms are packed with chemicals and will transferred from the Miller Freeman when the chemicals are unloaded. A spare copy of the MSDS forms will accompany the PI/student.

The Marine Nutrient Chemistry Laboratory (MNCL) analyzes the quantity of biogenic nutrients (orthophosphate, dissolved silicon, nitrate plus nitrite, nitrite, and ammonium) that are utilized by marine plants during growth and are excreted by microorganisms and marine animals. The quantity of nutrients, along with available light, determines the growth rate of marine plants such as phytoplankton and seagrasses. The principal nutrients measured are dissolved in marine, estuarine and fresh waters and are present in concentrations ranging from nannomoles to millimoles per liter. Clean seawater will contain a few micromoles of nutrients per liter so high purity distilled water and analytical grade chemicals are necessary to obtain the required accuracy and precision of the measurements.

The Alpkem model 300 chemical analyzers have separate chemical analysis manifolds so the chemicals from each analysis could be collected individually. This has not been done in the past since it was much more convenient to combine all of the waste streams into a single waste line. In the past this waste stream was directed to a normal laboratory drain which also received tap water wastes.

The table shows each individual chemical and the quantities of chemical solutions that are prepared. The second column has a value for grams of chemical/liter in the chemical reagent. The third column lists the pumping rate of each chemical reagent, water washes and samples. The last column shows the grams of each chemical in the waste of a typical 8 hour day.
 

At the bottom of the table, the total hourly volume of chemical reagents, washes and samples are shown. The daily volume was calculated for an 8 hour day.

ALPKEM Nutrient Analyzer Waste Discharges

(assume an 8 hr day) chemical g/l ml/hr g/day

*****************************************************************************

Orthophosphate

Molydate Solution

Ammonium Molybdate 17.0 g 8.5 2.2 0.15

Sulfuric Acid 200 ml 100 2.2 1.76ml

Potassium Antimony Tartrate 0.37 g .185 2.2 0.003

Sodium Lauryl Sulfate 0.3 .15 2.2 0.002

dilute to 2000 ml with distilled water

Ascorbic Solution

Ascorbic Acid 6 g 15 2.2 0.26

Acetone 200 ml 500 2.2 8.8ml

dilute to 400 ml with distilled water

Dilution Water

distilled water - - 30

Sample (seawater or freshwater) - - 12

******************************************************************************

Dissolved Silicon (Silicate)

Molybdate Solution

Ammonium Molybdate 10.8g 10 17.2 1.38

Sulfuric Acid 2.8 ml 2.8 17.2 .39ml

dilute to 1000 ml with distilled water

Tartaric Solution

Tartaric Acid 100 g 100 7.1 5.68

dilute to 1000 ml with distilled water

Stannous Solution

Stannous Chloride 40 g 400 4.4 14.1

Hydrochloric Acid 50 ml 500 4.4 17.6ml

dilute to 100 ml with distilled water

Sample (seawater or freshwater) - - 12

******************************************************************************

Nitrate plus Nitrite

Ammonium Solution

Ammonium Chloride 10 g 5 17.2 .69

dilute to 2000 ml with distilled water

Sulfanilamide Solution

sulfanilamide 10 g 10 4.4 .35

Hydrochloric Acid 100 ml 100 4.4 3.5ml

Brij-35 0.5 ml .5 4.4 .02mldilute to 1000 ml with distilled water

Naphthyl Solution

N-(1-Naphthyl)ethylenediamine- 1 g 1 4.4 .04

Dihydrochloride

Brij-35 0.5 ml .5 4.4 .02ml

dilute to 1000 ml with distilled water

Sample (seawater or freshwater) - - 8

******************************************************************************

Nitrite

Sulfanilamide Solution

sulfanilamide 10 g 10 4.4 .35

Hydrochloric Acid 100 ml 100 4.4 3.5ml

Brij-35 0.5 ml .5 4.4 .02ml

dilute to 1000 ml with distilled water

Naphthyl Solution

N-(1-Naphthyl)ethylenediamine- 1 g 1 4.4 .04

Dihydrochloride

Brij-35 0.5 ml .5 4.4 .02ml

dilute to 1000 ml with distilled water

Dilution Water

distilled water - - 8

Sample (seawater or freshwater) - - 30

******************************************************************************

Ammonium

Reagent A - Phenol Solution

Phenol 35 g 35 4.4 1.23

Sodium Nitroprusside Dihydrate 0.4 g .4 4.4 .01

dilute to 1000 ml with distilled water

Reagent B - Chlorox Solution

Chlorox (~5%) 50 ml 2.5 4.4 .09

Sodium Hydroxide 5 g 5 4.4 .18

dilute to 1000 ml with distilled water

Dilution Complex - Citrate Buffer

Sodium Citrate 140 g 140 13.6 15.2

Sodium Hydroxide 5 g 5 13.6 .54

dilute to 1000 ml with distilled water

Sample (seawater or freshwater) - - 30

******************************************************************************

Sampler Wash Water

Baseline Solution

Distilled Water - - 200 -

******************************************************************************

Total Solution Volume (ml/hour) - - 499.5

Total Solution Volume (ml/day) - - 3996

So there are 500 ml of waste solution/hour which translates to 3996 ml of waste solution per day.
 

9.3.2.1 Chemical Inventory Estimate - Dalhousie University
 

Investigator Name: Cullen Date: April 2000

Cruise ID: RB-OO-03, Leg 3 Cruise Dates:18-30 May 2000 Chief Scientist: E. D. Cokelet
 

Chemical Name: acetone Concentration: 99.9%	Owner: John Cullen	Sample Owner: John Cullen	Used for: pigment extraction	Quantity: 24L
	Hazard: highly flammable; irritant to respiratory system, skin and eyes	MSDS Provided? yes	Neutralizer: activated carbon adsorber	Protection equipment: fume hood; gloves; lab coat	Loaded: 24L
	Storage: flammable locker	Disposal Plan: waste will be removed in Seattle by PMEL personnel; remainder of chemicals to be stored for use next year	Off-loading: to be off loaded in Seattle by PMEL personnel	Over-packing:	Used: 18L
					Unloaded: 6L

Chemical Name: chlorophyll	Concentration: 100%	Owner: John Cullen	Sample Owner: John Cullen	Used for: fluorometer calibration	Quantity: 2 mg
	Hazard: none	MSDS Provided? yes	Neutralizer: n/a	Protection equipment: n/a	Loaded: 2 mg
	Storage: freezer	Disposal Plan: n/a	Off-loading: n/a	Over-packing:	Used: 2 mg
					Unloaded: n/a

Chemical Name: dimethyl sulfoxide	Concentration: 100%	Owner: John Cullen	Sample Owner: John Cullen	Used for: pigment extraction	Quantity: 3.8 L
	Hazard: combustible; irritant to respiratory system, skin and eyes	MSDS Provided? yes	Neutralizer: sand or vermiculite	Protection equipment: fume hood; respirator; rubber gloves and boots	Loaded: 3.8 L
	Storage: flammable locker	Disposal Plan: waste will be removed in Seattle by PMEL personnel; remainder of chemicals to be stored for use next year	Off-loading: to be off loaded in Seattle by PMEL personnel	Over-packing:	Used: 1.8 L
					Unloaded: 2 L

Chemical Name: glutaraldehyde	Concentration: 50%	Owner: John Cullen	Sample Owner: John Cullen	Used for: sea-water sample preservation	Quantity: 100 ml
	Hazard: highly toxic; harmful in contact with skin	MSDS Provided? yes	Neutralizer: dry lime or soda ash	Protection equipment: fume hood; respirator; rubber gloves and boots	Loaded: 100 ml
	Storage: cool dry place	Disposal Plan: waste will be removed in Seattle by PMEL personnel; remainder of chemicals to be stored for use next year	Off-loading: to be off loaded in Seattle by PMEL personnel	Over-packing:	Used: 30 ml
					Unloaded: 70 ml

Chemical Name: hydrochloric acid	Concentration: 36%	Owner: John Cullen	Sample Owner: John Cullen	Used for: acidification of chlorophyll samples	Quantity: 1 L
	Hazard: corrosive; toxic by inhalation or swallowed; reacts violently with water	MSDS Provided? yes	Neutralizer: dry lime, sand or soda ash	Protection equipment: fume hood; respirator; rubber gloves and boots	Loaded: l L
	Storage: corrosive locker	Disposal Plan: waste will be removed in Seattle by PMEL personnel; remainder of chemicals to be stored for use next year	Off-loading: to be off loaded in Seattle by PMEL personnel	Over-packing:	Used: 0.2 L
					Unloaded: 0.8 L

Chemical Name: methanol	Concentration: 100%	Owner: John Cullen	Sample Owner: John Cullen	Used for: instrument cleaning	Quantity: 2 L
	Hazard: highly flammable; toxic if inhaled or swallowed; irritant to eyes and skin	MSDS Provided? yes	Neutralizer: dry lime, sand or soda ash	Protection equipment: fume hood; respirator; rubber gloves and boots	Loaded: 2 L
	Storage: flammable locker	Disposal Plan: waste will be removed in Seattle by PMEL personnel; remainder of chemicals to be stored for use next year	Off-loading: to be off loaded in Seattle by PMEL personnel	Over-packing:	Used: 0.5 L
					Unloaded: 1.5 L

9.3.2.2.1 MATERIAL SAFETY DATA SHEET

SECTION 1. - - - - - - - - - CHEMICAL IDENTIFICATION- - - - - - - - - -

CATALOG #: C6144

NAME: CHLOROPHYLL A FROM ANACYSTIS

NIDULANS ALGAE

SECTION 2. - - - - - COMPOSITION/INFORMATION ON INGREDIENTS - - - - - -

CAS #: 479-61-8

MF: C55H72MGN4O5

EC NO: 207-536-6

SYNONYMS

CHLOROFOLIN * CHLOROFYL (CZECH) * CHLOROPHYLLS * C.I. 1956 *

DEODOPHYLL * E 140 * L-GRUEN NO. 1 (GERMAN) *

SECTION 3. - - - - - - - - - - HAZARDS IDENTIFICATION - - - - - - - - -

LABEL PRECAUTIONARY STATEMENTS

CAUTION:

AVOID CONTACT AND INHALATION.

SECTION 4. - - - - - - - - - - FIRST-AID MEASURES- - - - - - - - - - -

IN CASE OF CONTACT, IMMEDIATELY FLUSH EYES WITH COPIOUS AMOUNTS OF

WATER FOR AT LEAST 15 MINUTES.

IN CASE OF CONTACT, IMMEDIATELY WASH SKIN WITH SOAP AND COPIOUS

AMOUNTS OF WATER.

IF INHALED, REMOVE TO FRESH AIR. IF NOT BREATHING GIVE ARTIFICIAL

RESPIRATION. IF BREATHING IS DIFFICULT, GIVE OXYGEN.

IF SWALLOWED, WASH OUT MOUTH WITH WATER PROVIDED PERSON IS CONSCIOUS.

CALL A PHYSICIAN.

WASH CONTAMINATED CLOTHING BEFORE REUSE.

SECTION 5. - - - - - - - - - FIRE FIGHTING MEASURES - - - - - - - - - -

EXTINGUISHING MEDIA

WATER SPRAY.

CARBON DIOXIDE, DRY CHEMICAL POWDER OR APPROPRIATE FOAM.

SPECIAL FIREFIGHTING PROCEDURES

WEAR SELF-CONTAINED BREATHING APPARATUS AND PROTECTIVE CLOTHING TO

PREVENT CONTACT WITH SKIN AND EYES.

UNUSUAL FIRE AND EXPLOSIONS HAZARDS

EMITS TOXIC FUMES UNDER FIRE CONDITIONS.

SECTION 6. - - - - - - - - ACCIDENTAL RELEASE MEASURES- - - - - - - - -

WEAR RESPIRATOR, CHEMICAL SAFETY GOGGLES, RUBBER BOOTS AND HEAVY

RUBBER GLOVES.

SWEEP UP, PLACE IN A BAG AND HOLD FOR WASTE DISPOSAL.

AVOID RAISING DUST.

VENTILATE AREA AND WASH SPILL SITE AFTER MATERIAL PICKUP IS COMPLETE.

SECTION 7. - - - - - - - - - - HANDLING AND STORAGE- - - - - - - - - - -

REFER TO SECTION 8.

SECTION 8. - - - - - - EXPOSURE CONTROLS/PERSONAL PROTECTION- - - - - -

CHEMICAL SAFETY GOGGLES.

NIOSH/MSHA-APPROVED RESPIRATOR.

MECHANICAL EXHAUST REQUIRED.

COMPATIBLE CHEMICAL-RESISTANT GLOVES.

AVOID BREATHING DUST.

AVOID CONTACT WITH EYES, SKIN AND CLOTHING.

WASH THOROUGHLY AFTER HANDLING.

KEEP CONTAINER CLOSED.

LIGHT SENSITIVE

REFRIGERATE.

SECTION 9. - - - - - - - PHYSICAL AND CHEMICAL PROPERTIES - - - - - - -

APPEARANCE AND ODOR

GREEN TO BLUE POWDER OR CRYSTALS

PHYSICAL PROPERTIES

MELTING POINT: 150 C TO 153 C (DEC)

SECTION 10. - - - - - - - - -STABILITY AND REACTIVITY - - - - - - - - -

INCOMPATIBILITIES

STRONG OXIDIZING AGENTS

MAY DISCOLOR ON EXPOSURE TO LIGHT.

HAZARDOUS COMBUSTION OR DECOMPOSITION PRODUCTS

TOXIC FUMES OF:

CARBON MONOXIDE, CARBON DIOXIDE

NITROGEN OXIDES

SECTION 11. - - - - - - - - - TOXICOLOGICAL INFORMATION - - - - - - - -

ACUTE EFFECTS

MAY BE HARMFUL BY INHALATION, INGESTION, OR SKIN ABSORPTION.

MAY CAUSE IRRITATION.

TO THE BEST OF OUR KNOWLEDGE, THE CHEMICAL, PHYSICAL, AND

TOXICOLOGICAL PROPERTIES HAVE NOT BEEN THOROUGHLY INVESTIGATED.

RTECS #: FW6420000

CHLOROPHYLL

TOXICITY DATA

IPR-RAT LD50:>100 MG/KG NIIRDN -,380,1990

IVN-GPG LD50:85 MG/KG ARZNAD 7,357,1957

ONLY SELECTED REGISTRY OF TOXIC EFFECTS OF CHEMICAL SUBSTANCES

(RTECS) DATA IS PRESENTED HERE. SEE ACTUAL ENTRY IN RTECS FOR

COMPLETE INFORMATION.

SECTION 12. - - - - - - - - - ECOLOGICAL INFORMATION - - - - - - - - - -

DATA NOT YET AVAILABLE.

SECTION 13. - - - - - - - - - DISPOSAL CONSIDERATIONS - - - - - - - - -

DISSOLVE OR MIX THE MATERIAL WITH A COMBUSTIBLE SOLVENT AND BURN IN A

CHEMICAL INCINERATOR EQUIPPED WITH AN AFTERBURNER AND SCRUBBER.

OBSERVE ALL FEDERAL, STATE AND LOCAL ENVIRONMENTAL REGULATIONS.

SECTION 14. - - - - - - - - - - TRANSPORT INFORMATION - - - - - - - - -

CONTACT SIGMA CHEMICAL COMPANY FOR TRANSPORTATION INFORMATION.

SECTION 15. - - - - - - - - - REGULATORY INFORMATION - - - - - - - - - -

DATA NOT AVAILABLE

SECTION 16. - - - - - - - - - - OTHER INFORMATION- - - - - - - - - - - -

THE ABOVE INFORMATION IS BELIEVED TO BE CORRECT BUT DOES NOT PURPORT TO

BE ALL INCLUSIVE AND SHALL BE USED ONLY AS A GUIDE. SIGMA, ALDRICH,

FLUKA SHALL NOT BE HELD LIABLE FOR ANY DAMAGE RESULTING FROM HANDLING

OR FROM CONTACT WITH THE ABOVE PRODUCT. SEE REVERSE SIDE OF INVOICE OR

PACKING SLIP FOR ADDITIONAL TERMS AND CONDITIONS OF SALE.

COPYRIGHT 1999 SIGMA-ALDRICH CO.

LICENSE GRANTED TO MAKE UNLIMITED PAPER COPIES FOR INTERNAL USE ONLY

SECTION 1. - - - - - - - - - CHEMICAL IDENTIFICATION- - - - - - - - - -

CATALOG #: D8779

NAME: DIMETHYL SULFOXIDE ACS REAGENT

SECTION 2. - - - - - COMPOSITION/INFORMATION ON INGREDIENTS - - - - - -

CAS #: 67-68-5

MF: C2H6OS

EC NO: 200-664-3

SYNONYMS

A 10846 * DELTAN * DEMESO * DEMASORB * DEMAVET * DEMSODROX *

DERMASORB * DIMETHYL SULFOXIDE * DIMETHYL SULPHOXIDE * DIMEXIDE *

DIPIRARTRIL-TROPICO * DMS-70 * DMS-90 * DMSO * DOLICUR * DOMOSO *

DROMISOL * DURASORB * GAMASOL 90 * HYADUR * INFILTRINA * M 176 *

METHANE, SULFINYLBIS- * METHYLSULFINYLMETHANE * NSC-763 * RIMSO-50 *

SOMIPRONT * SQ 9453 * SULFINYLBIS(METHANE) * SYNTEXAN * TOPSYM *

SECTION 3. - - - - - - - - - - HAZARDS IDENTIFICATION - - - - - - - - -

LABEL PRECAUTIONARY STATEMENTS

IRRITANT

IRRITATING TO EYES, RESPIRATORY SYSTEM AND SKIN.

COMBUSTIBLE.

READILY ABSORBED THROUGH SKIN.

TARGET ORGAN(S):

EYES

SKIN

IN CASE OF CONTACT WITH EYES, RINSE IMMEDIATELY WITH PLENTY OF

WATER AND SEEK MEDICAL ADVICE.

WEAR SUITABLE PROTECTIVE CLOTHING.

DO NOT BREATHE VAPOR.

SECTION 4. - - - - - - - - - - FIRST-AID MEASURES- - - - - - - - - - -

IF SWALLOWED, WASH OUT MOUTH WITH WATER PROVIDED PERSON IS CONSCIOUS.

CALL A PHYSICIAN.

IF INHALED, REMOVE TO FRESH AIR. IF NOT BREATHING GIVE ARTIFICIAL

RESPIRATION. IF BREATHING IS DIFFICULT, GIVE OXYGEN.

IN CASE OF CONTACT, IMMEDIATELY WASH SKIN WITH SOAP AND COPIOUS

AMOUNTS OF WATER.

IN CASE OF CONTACT, IMMEDIATELY FLUSH EYES WITH COPIOUS AMOUNTS OF

WATER FOR AT LEAST 15 MINUTES.

SECTION 5. - - - - - - - - - FIRE FIGHTING MEASURES - - - - - - - - - -

EXTINGUISHING MEDIA

WATER SPRAY.

CARBON DIOXIDE, DRY CHEMICAL POWDER OR APPROPRIATE FOAM.

SPECIAL FIREFIGHTING PROCEDURES

WEAR SELF-CONTAINED BREATHING APPARATUS AND PROTECTIVE CLOTHING TO

PREVENT CONTACT WITH SKIN AND EYES.

UNUSUAL FIRE AND EXPLOSIONS HAZARDS

COMBUSTIBLE LIQUID.

EMITS TOXIC FUMES UNDER FIRE CONDITIONS.

METHYL SULFOXIDE (DMSO) UNDERGOES A VIOLENT EXOTHERMIC REACTION ON

MIXING WITH COPPER WOOL AND TRICHLOROACETIC ACID. ON MIXING WITH

POTASSIUM PERMANGANATE IT WILL FLASH INSTANTANEOUSLY. IT REACTS

VIOLENTLY WITH: ACID HALIDES, CYANURIC CHLORIDE, SILICON TETRACHLORIDE,

PHOSPHORUS TRICHLORIDE AND TRIOXIDE, THIONYL CHLORIDE, MAGNESIUM

PERCHLORATE, SILVER FLUORIDE, METHYL BROMIDE, IODINE PENTAFLUORIDE,

NITROGEN PERIODATE, DIBORANE, SODIUM HYDRIDE, PERCHLORIC AND PERIODIC

ACIDS. WHEN HEATED ABOVE ITS BOILING POINT METHYL SULFOXIDE DEGRADES

GIVING OFF FORMALDEHYDE, METHYL MERCAPTAN, AND SULFUR DIOXIDE.

SECTION 6. - - - - - - - - ACCIDENTAL RELEASE MEASURES- - - - - - - - -

WEAR RESPIRATOR, CHEMICAL SAFETY GOGGLES, RUBBER BOOTS AND HEAVY

RUBBER GLOVES.

ABSORB ON SAND OR VERMICULITE AND PLACE IN CLOSED CONTAINERS FOR

DISPOSAL.

VENTILATE AREA AND WASH SPILL SITE AFTER MATERIAL PICKUP IS COMPLETE.

EVACUATE AREA.

SECTION 7. - - - - - - - - - - HANDLING AND STORAGE- - - - - - - - - - -

REFER TO SECTION 8.

SECTION 8. - - - - - - EXPOSURE CONTROLS/PERSONAL PROTECTION- - - - - -

MECHANICAL EXHAUST REQUIRED.

SAFETY SHOWER AND EYE BATH.

WASH THOROUGHLY AFTER HANDLING.

NIOSH/MSHA-APPROVED RESPIRATOR.

COMPATIBLE CHEMICAL-RESISTANT GLOVES.

CHEMICAL SAFETY GOGGLES.

DO NOT BREATHE VAPOR.

AVOID CONTACT WITH DMSO SOLUTIONS CONTAINING TOXIC MATERIALS OR

MATERIALS WITH UNKNOWN TOXICOLOGICAL PROPERTIES. DIMETHYL SULFOXIDE

IS READILY ABSORBED THROUGH SKIN AND MAY CARRY SUCH MATERIALS INTO

THE BODY.

AVOID PROLONGED OR REPEATED EXPOSURE.

STORE IN A COOL DRY PLACE.

KEEP AWAY FROM HEAT AND OPEN FLAME.

KEEP TIGHTLY CLOSED.

SECTION 9. - - - - - - - PHYSICAL AND CHEMICAL PROPERTIES - - - - - - -

PHYSICAL PROPERTIES

BOILING POINT: 189 C

MELTING POINT: 18,4 C

FLASHPOINT 188,6 F

87 C

EXPLOSION LIMITS IN AIR:

LOWER 42 %

VAPOR PRESSURE: 0,42 MMHG

SPECIFIC GRAVITY: 1,1 G

VAPOR DENSITY: 2,7 G/L

SECTION 10. - - - - - - - - -STABILITY AND REACTIVITY - - - - - - - - -

STABILITY

STABLE.

INCOMPATIBILITIES

SENSITIVE TO MOISTURE

ACID CHLORIDES

PHOSPHORUS HALIDES

STRONG ACIDS

STRONG OXIDIZING AGENTS

STRONG REDUCING AGENTS

HAZARDOUS COMBUSTION OR DECOMPOSITION PRODUCTS

CARBON MONOXIDE, CARBON DIOXIDE

SULFUR OXIDES

HAZARDOUS POLYMERIZATION

WILL NOT OCCUR.

SECTION 11. - - - - - - - - - TOXICOLOGICAL INFORMATION - - - - - - - -

ACUTE EFFECTS

CAUSES SKIN IRRITATION.

READILY ABSORBED THROUGH SKIN.

CAUSES EYE IRRITATION.

MATERIAL IS IRRITATING TO MUCOUS MEMBRANES AND UPPER

RESPIRATORY TRACT.

MAY BE HARMFUL BY INHALATION, INGESTION, OR SKIN ABSORPTION.

TO THE BEST OF OUR KNOWLEDGE, THE CHEMICAL, PHYSICAL, AND

TOXICOLOGICAL PROPERTIES HAVE NOT BEEN THOROUGHLY INVESTIGATED.

AVOID CONTACT WITH DMSO SOLUTIONS CONTAINING TOXIC MATERIALS OR

MATERIALS WITH UNKNOWN TOXICOLOGICAL PROPERTIES. DIMETHYL SULFOXIDE

IS READILY ABSORBED THROUGH SKIN AND MAY CARRY SUCH MATERIALS INTO

THE BODY.

CHRONIC EFFECTS

TARGET ORGAN(S):

EYES

SKIN

RTECS #: PV6210000

METHYL SULFOXIDE

IRRITATION DATA

SKN-RBT 10 MG/24H OPEN MLD AIHAAP 23,95,1962

SKN-RBT 500 MG/24H MLD 85JCAE -,1044,1986

EYE-RBT 100 MG TXAPA9 39,129,1977

EYE-RBT 500 MG/24H MLD 85JCAE -,1044,1986

TOXICITY DATA

ORL-RAT LD50:14500 MG/KG TXAPA9 15,74,1969

SKN-RAT LD50:40 GM/KG ANYAA9 141,96,1967

IPR-RAT LD50:8200 MG/KG FCTOD7 22,665,1984

SCU-RAT LD50:12 GM/KG ARZNAD 14,1050,1964

IVN-RAT LD50:5360 MG/KG TXAPA9 7,104,1965

UNR-RAT LD50:1300 MG/KG NTIS** AD-A159-418

ORL-MUS LD50:7920 MG/KG CHTPBA 3,10,1968

SKN-MUS LD50:50 GM/KG ANYAA9 141,96,1967

IPR-MUS LD50:2500 MG/KG RPTOAN 35,300,1972

SCU-MUS LD50:14 GM/KG ANYAA9 141,96,1967

IVN-MUS LD50:3100 MG/KG TXAPA9 15,74,1969

UNR-MUS LD50:12 GM/KG USXXAM #4767763

ORL-DOG LD50:>10 GM/KG ANYAA9 141,96,1967

IVN-DOG LD50:2500 MG/KG CNCRA6 31,7,1963

ORL-CKN LD50:12 GM/KG JPPMAB 15,688,1963

ORL-MAM LD50:21400 MG/KG GISAAA 39(4),86,1974

ORL-BWD LD50:100 MG/KG TXAPA9 21,315,1972

TARGET ORGAN DATA

BEHAVIORAL (ALTERED SLEEP TIME)

LUNGS, THORAX OR RESPIRATION (DYSPNAE)

LUNGS, THORAX OR RESPIRATION (CYANOSIS)

GASTROINTESTINAL (NAUSEA OR VOMITING)

LIVER (JAUNDICE, OTHER OR UNCLASSIFIED)

BLOOD (OTHER CHANGES)

EFFECTS ON FERTILITY (PRE-IMPLANTATION MORTALITY)

EFFECTS ON EMBRYO OR FETUS (FETOTOXICITY)

SPECIFIC DEVELOPMENTAL ABNORMALITIES (MUSCULOSKELETAL SYSTEM)

ONLY SELECTED REGISTRY OF TOXIC EFFECTS OF CHEMICAL SUBSTANCES

(RTECS) DATA IS PRESENTED HERE. SEE ACTUAL ENTRY IN RTECS FOR

COMPLETE INFORMATION.

SECTION 12. - - - - - - - - - ECOLOGICAL INFORMATION - - - - - - - - - -

DATA NOT YET AVAILABLE.

SECTION 13. - - - - - - - - - DISPOSAL CONSIDERATIONS - - - - - - - - -

THIS COMBUSTIBLE MATERIAL MAY BE BURNED IN A CHEMICAL INCINERATOR

EQUIPPED WITH AN AFTERBURNER AND SCRUBBER.

OBSERVE ALL FEDERAL, STATE AND LOCAL ENVIRONMENTAL REGULATIONS.

SECTION 14. - - - - - - - - - - TRANSPORT INFORMATION - - - - - - - - -

CONTACT SIGMA CHEMICAL COMPANY FOR TRANSPORTATION INFORMATION.

SECTION 15. - - - - - - - - - REGULATORY INFORMATION - - - - - - - - - -

EUROPEAN INFORMATION

IRRITANT

R 36/37/38

IRRITATING TO EYES, RESPIRATORY SYSTEM AND SKIN.

S 26

IN CASE OF CONTACT WITH EYES, RINSE IMMEDIATELY WITH PLENTY OF

WATER AND SEEK MEDICAL ADVICE.

S 36

WEAR SUITABLE PROTECTIVE CLOTHING.

S 23

DO NOT BREATHE VAPOR.

REVIEWS, STANDARDS, AND REGULATIONS

OEL=MAK

OEL-RUSSIA:STEL 20 MG/M3 JAN 1993

OEL-SWITZERLAND:TWA 50 PPM (160 MG/M3);SKIN JAN 1993

OEL-SWEDEN: TWA 50 PPM (150 MG/M3), KTV 150 PPM (500 MG/M3), SKIN,

JAN1999

NOHS 1974: HZD 80564; NIS 11; TNF 476; NOS 25; TNE 22461

NOES 1983: HZD 80564; NIS 29; TNF 3507; NOS 40; TNE 44947; TFE 16837

EPA GENETOX PROGRAM 1988, POSITIVE: ASPERGILLUS-ANEUPLOIDY; S

CEREVISIAE GENE CONVERSION

EPA GENETOX PROGRAM 1988, NEGATIVE: SHE-CLONAL ASSAY; CELL

TRANSFORM.-MOUSE EMBRYO

EPA GENETOX PROGRAM 1988, NEGATIVE: CELL TRANSFORM.-RLV F344 RAT EMBRYO

EPA GENETOX PROGRAM 1988, NEGATIVE: D MELANOGASTER-WHOLE SEX CHROM.

LOSS; HOST-MEDIATED ASSAY

EPA GENETOX PROGRAM 1988, NEGATIVE: N CRASSA-ANEUPLOIDY; E COLI POLA

WITH S9

EPA GENETOX PROGRAM 1988, NEGATIVE: HISTIDINE REVERSION-AMES TEST; IN

VITRO SCE-NONHUMAN

EPA GENETOX PROGRAM 1988, NEGATIVE: D MELANOGASTER SEX-LINKED LETHAL

EPA GENETOX PROGRAM 1988, INCONCLUSIVE: ASPERGILLUS-RECOMBINATION;

CARCINOGENICITY-MOUSE/RAT

EPA GENETOX PROGRAM 1988, INCONCLUSIVE: D MELANOGASTER-RECIPROCAL

TRANSLOCATION

EPA GENETOX PROGRAM 1988, INCONCLUSIVE: RODENT DOMINANT LETHAL; B

SUBTILIS REC ASSAY

EPA GENETOX PROGRAM 1988, INCONCLUSIVE: E COLI POLA WITHOUT S9

EPA TSCA SECTION 8(B) CHEMICAL INVENTORY

EPA TSCA SECTION 8(D) UNPUBLISHED HEALTH/SAFETY STUDIES

EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, SEPTEMBER 1999

SECTION 16. - - - - - - - - - - OTHER INFORMATION- - - - - - - - - - - -

THE ABOVE INFORMATION IS BELIEVED TO BE CORRECT BUT DOES NOT PURPORT TO

BE ALL INCLUSIVE AND SHALL BE USED ONLY AS A GUIDE. SIGMA, ALDRICH,

FLUKA SHALL NOT BE HELD LIABLE FOR ANY DAMAGE RESULTING FROM HANDLING

OR FROM CONTACT WITH THE ABOVE PRODUCT. SEE REVERSE SIDE OF INVOICE OR

PACKING SLIP FOR ADDITIONAL TERMS AND CONDITIONS OF SALE.

COPYRIGHT 1999 SIGMA-ALDRICH CO.

LICENSE GRANTED TO MAKE UNLIMITED PAPER COPIES FOR INTERNAL USE ONLY

SECTION 1. - - - - - - - - - CHEMICAL IDENTIFICATION- - - - - - - - - -

CATALOG #: G6403

NAME: GLUTARALDEHYDE 50% AQUEOUS SOLUTION

PHOTOGRAPHIC GRADE

SECTION 2. - - - - - COMPOSITION/INFORMATION ON INGREDIENTS - - - - - -

CAS #: 111-30-8

MF: C5H8O2

EC NO: 203-856-5

SYNONYMS

ALDEHYD GLUTAROWY (POLISH) * CIDEX * 1,3-DIFORMYLPROPANE * GLUTARAL *

GLUTARALDEHYD (CZECH) * GLUTARALDEHYDE (ACGIH) * GLUTARDIALDEHYDE *

GLUTARIC DIALDEHYDE * NCI-C55425 * 1,5-PENTANEDIAL * 1,5-PENTANEDIONE

* POTENTIATED ACID GLUTARALDEHYDE * SONACIDE *

SECTION 3. - - - - - - - - - - HAZARDS IDENTIFICATION - - - - - - - - -

LABEL PRECAUTIONARY STATEMENTS

HIGHLY TOXIC (USA)

VERY TOXIC (EU)

VERY TOXIC BY INHALATION.

TOXIC IF SWALLOWED.

HARMFUL IN CONTACT WITH SKIN.

CAUSES BURNS.

MAY CAUSE SENSITIZATION BY SKIN CONTACT.

POSSIBLE RISK OF IRREVERSIBLE EFFECTS.

TARGET ORGAN(S):

CENTRAL NERVOUS SYSTEM

HEART

IN CASE OF ACCIDENT OR IF YOU FEEL UNWELL, SEEK MEDICAL ADVICE

IMMEDIATELY (SHOW THE LABEL WHERE POSSIBLE).

IN CASE OF CONTACT WITH EYES, RINSE IMMEDIATELY WITH PLENTY OF

WATER AND SEEK MEDICAL ADVICE.

WEAR SUITABLE PROTECTIVE CLOTHING, GLOVES AND EYE/FACE

PROTECTION.

DO NOT BREATHE VAPOR.

SECTION 4. - - - - - - - - - - FIRST-AID MEASURES- - - - - - - - - - -

IN CASE OF CONTACT, IMMEDIATELY FLUSH EYES OR SKIN WITH COPIOUS

AMOUNTS OF WATER FOR AT LEAST 15 MINUTES WHILE REMOVING CONTAMINATED

CLOTHING AND SHOES.

ASSURE ADEQUATE FLUSHING OF THE EYES BY SEPARATING THE EYELIDS

WITH FINGERS.

IF INHALED, REMOVE TO FRESH AIR. IF NOT BREATHING GIVE ARTIFICIAL

RESPIRATION. IF BREATHING IS DIFFICULT, GIVE OXYGEN.

IF SWALLOWED, WASH OUT MOUTH WITH WATER PROVIDED PERSON IS CONSCIOUS.

CALL A PHYSICIAN.

WASH CONTAMINATED CLOTHING BEFORE REUSE.

SECTION 5. - - - - - - - - - FIRE FIGHTING MEASURES - - - - - - - - - -

EXTINGUISHING MEDIA

WATER SPRAY.

CARBON DIOXIDE, DRY CHEMICAL POWDER OR APPROPRIATE FOAM.

SPECIAL FIREFIGHTING PROCEDURES

WEAR SELF-CONTAINED BREATHING APPARATUS AND PROTECTIVE CLOTHING TO

PREVENT CONTACT WITH SKIN AND EYES.

UNUSUAL FIRE AND EXPLOSIONS HAZARDS

EMITS TOXIC FUMES UNDER FIRE CONDITIONS.

SECTION 6. - - - - - - - - ACCIDENTAL RELEASE MEASURES- - - - - - - - -

EVACUATE AREA.

WEAR SELF-CONTAINED BREATHING APPARATUS, RUBBER BOOTS AND HEAVY

RUBBER GLOVES.

SHUT OFF ALL SOURCES OF IGNITION.

COVER WITH DRY LIME OR SODA ASH, PICK UP, KEEP IN A CLOSED CONTAINER

AND HOLD FOR WASTE DISPOSAL.

VENTILATE AREA AND WASH SPILL SITE AFTER MATERIAL PICKUP IS COMPLETE.

SECTION 7. - - - - - - - - - - HANDLING AND STORAGE- - - - - - - - - - -

REFER TO SECTION 8.

SECTION 8. - - - - - - EXPOSURE CONTROLS/PERSONAL PROTECTION- - - - - -

WEAR APPROPRIATE NIOSH/MSHA-APPROVED RESPIRATOR, CHEMICAL-RESISTANT

GLOVES, SAFETY GOGGLES, OTHER PROTECTIVE CLOTHING.

USE ONLY IN A CHEMICAL FUME HOOD.

SAFETY SHOWER AND EYE BATH.

FACESHIELD (8-INCH MINIMUM).

DO NOT BREATHE VAPOR.

DO NOT GET IN EYES, ON SKIN, ON CLOTHING.

AVOID PROLONGED OR REPEATED EXPOSURE.

WASH THOROUGHLY AFTER HANDLING.

KEEP TIGHTLY CLOSED.

STORE IN A COOL DRY PLACE.

SECTION 9. - - - - - - - PHYSICAL AND CHEMICAL PROPERTIES - - - - - - -

APPEARANCE AND ODOR

LIQUID.

PHYSICAL PROPERTIES

VAPOR PRESSURE: 15MM 20 C

SPECIFIC GRAVITY: 1.106

VAPOR DENSITY: 1.05

SECTION 10. - - - - - - - - -STABILITY AND REACTIVITY - - - - - - - - -

STABILITY

STABLE.

INCOMPATIBILITIES

STRONG ACIDS

STRONG BASES

STRONG OXIDIZING AGENTS

MAY DISCOLOR ON EXPOSURE TO AIR.

HAZARDOUS COMBUSTION OR DECOMPOSITION PRODUCTS

TOXIC FUMES OF:

CARBON MONOXIDE, CARBON DIOXIDE

HAZARDOUS POLYMERIZATION

NONHAZARDOUS POLYMERIZATION MAY OCCUR. AVOID INITIATORS, ACCELERATORS,

HEAT, PRESSURE, CONTAMINATION.

SECTION 11. - - - - - - - - - TOXICOLOGICAL INFORMATION - - - - - - - -

ACUTE EFFECTS

MAY BE FATAL IF INHALED.

TOXIC IF SWALLOWED.

HARMFUL IF ABSORBED THROUGH SKIN.

MATERIAL IS EXTREMELY DESTRUCTIVE TO TISSUE OF THE MUCOUS MEMBRANES

AND UPPER RESPIRATORY TRACT, EYES AND SKIN.

INHALATION MAY RESULT IN SPASM, INFLAMMATION AND EDEMA OF THE

LARYNX AND BRONCHI, CHEMICAL PNEUMONITIS AND PULMONARY EDEMA.

SYMPTOMS OF EXPOSURE MAY INCLUDE BURNING SENSATION, COUGHING,

WHEEZING, LARYNGITIS, SHORTNESS OF BREATH, HEADACHE, NAUSEA AND

VOMITING.

MAY CAUSE ALLERGIC SKIN REACTION.

CHRONIC EFFECTS

LABORATORY EXPERIMENTS HAVE SHOWN MUTAGENIC EFFECTS.

TARGET ORGAN(S):

CENTRAL NERVOUS SYSTEM

HEART

THE TOXICOLOGICAL PROPERTIES HAVE NOT BEEN THOROUGHLY

INVESTIGATED.

RTECS #: MA2450000

GLUTARALDEHYDE

IRRITATION DATA

SKN-HMN 6 MG/3D-I SEV 85DKA8 -,127,1977

SKN-RBT 13 MG OPEN MLD UCDS** 1/30/1970

SKN-RBT 2 MG/24H SEV 85JCAE -,272,1986

EYE-RBT 1 MG SEV UCDS** 1/30/1970

EYE-RBT 250 UG/24H SEV 85JCAE -,272,1986

TOXICITY DATA

ORL-RAT LD50:134 MG/KG OYYAA2 19,503,1980

IHL-RAT LC50:480 MG/M3/4H EPASR* 8EHQ-1290-1008

SKN-RAT LD50:>2500 MG/KG OYYAA2 12,11,1976

IPR-RAT LD50:17900 UG/KG IYKEDH 10,232,1979

SCU-RAT LD50:>750 MG/KG NIIRDN -,395,1995

IVN-RAT LD50:9800 UG/KG EPASR* 8EHQ-1290-1008

ORL-MUS LD50:100 MG/KG OYYAA2 19,503,1980

SKN-MUS LD50:>5840 MG/KG OYYAA2 12,11,1976

IPR-MUS LD50:13900 UG/KG IYKEDH 10,232,1979

SCU-MUS LD50:>590 MG/KG NIIRDN -,395,1995

IVN-MUS LD50:15400 UG/KG OYYAA2 19,503,1980

SKN-RBT LD50:560 UL/KG UCDS** 11/4/1971

ORL-GPG LD50:50 MG/KG GISAAA 52(3),77,1987

ORL-DCK LD50:820 MG/KG NTIS** OTS0526410-1

TARGET ORGAN DATA

BEHAVIORAL (ALTERED SLEEP TIME)

BEHAVIORAL (SOMNOLENCE)

BEHAVIORAL (EXCITEMENT)

BEHAVIORAL (FOOD INTAKE)

PATERNAL EFFECTS (TESTES, EPIDIDYMIS, SPERM DUCT)

PATERNAL EFFECTS (PROSTATE, SEMINAL VESICLE, COWPER'S, ACCESSORY GLANDS

EFFECTS ON EMBRYO OR FETUS (FETOTOXICITY)

ONLY SELECTED REGISTRY OF TOXIC EFFECTS OF CHEMICAL SUBSTANCES

(RTECS) DATA IS PRESENTED HERE. SEE ACTUAL ENTRY IN RTECS FOR

COMPLETE INFORMATION.

SECTION 12. - - - - - - - - - ECOLOGICAL INFORMATION - - - - - - - - - -

DATA NOT YET AVAILABLE.

SECTION 13. - - - - - - - - - DISPOSAL CONSIDERATIONS - - - - - - - - -

DISSOLVE OR MIX THE MATERIAL WITH A COMBUSTIBLE SOLVENT AND BURN IN A

CHEMICAL INCINERATOR EQUIPPED WITH AN AFTERBURNER AND SCRUBBER.

OBSERVE ALL FEDERAL, STATE AND LOCAL ENVIRONMENTAL REGULATIONS.

SECTION 14. - - - - - - - - - - TRANSPORT INFORMATION - - - - - - - - -

CONTACT SIGMA CHEMICAL COMPANY FOR TRANSPORTATION INFORMATION.

SECTION 15. - - - - - - - - - REGULATORY INFORMATION - - - - - - - - - -

EUROPEAN INFORMATION

VERY TOXIC

R 23/25

TOXIC BY INHALATION AND IF SWALLOWED.

R 34

CAUSES BURNS.

R 42/43

MAY CAUSE SENSITIZATION BY INHALATION AND SKIN CONTACT.

R 50

VERY TOXIC TO AQUATIC ORGANISMS.

S 26

IN CASE OF CONTACT WITH EYES, RINSE IMMEDIATELY WITH PLENTY OF

WATER AND SEEK MEDICAL ADVICE.

S 36/37/39

WEAR SUITABLE PROTECTIVE CLOTHING, GLOVES AND EYE/FACE

PROTECTION.

S 45

IN CASE OF ACCIDENT OR IF YOU FEEL UNWELL, SEEK MEDICAL ADVICE

IMMEDIATELY (SHOW THE LABEL WHERE POSSIBLE).

S 61

AVOID RELEASE TO THE ENVIRONMENT. REFER TO SPECIAL INSTRUCTIONS/

SAFETY DATA SHEETS.

REVIEWS, STANDARDS, AND REGULATIONS

OEL=MAK

ACGIH TLV-CL 0.2 MG/M3 (0.05 PPM) DTLVS* TLV/BEI,1997

EPA FIFRA 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTRATION

FEREAC 54,7740,1989

OEL-AUSTRALIA:TWA 0.2 PPM (0.7 MG/M3) JAN 1993

OEL-BELGIUM:TWA 0.2 MG/M3;STEL 0.82 MG/M3 JAN 1993

OEL-FINLAND:STEL 0.2 PPM (0.8 MG/M3) JAN 1993

OEL-GERMANY:TWA 0.2 PPM (0.8 MG/M3) JAN 1993

OEL-THE NETHERLANDS:TWA 0.25 MG/M3 JAN 1993

OEL-RUSSIA:STEL 5 MG/M3 JAN 1993

OEL-SWEDEN:STEL 0.2 PPM (0.8 MG/M3) JAN 1993

OEL-SWITZERLAND:TWA 0.2 PPM (0.8 MG/M3);STEL 0.4 PPM (1.6 MG/M3) JAN

1993

OEL-UNITED KINGDOM:TWA 0.2 PPM (0.7 MG/M3);STEL 0.2 PPM JAN 1993

OEL IN BULGARIA, COLOMBIA, JORDAN, KOREA CHECK ACGIH TLV

OEL IN NEW ZEALAND, SINGAPORE, VIETNAM CHECK ACGIH TLV

NIOSH REL TO GLUTARALDEHYDE-AIR:CL 0.2 PPM

NIOSH* DHHS #92-100,1992

NOHS 1974: HZD 84349; NIS 11; TNF 2069; NOS 25; TNE 12954

NOES 1983: HZD 84349; NIS 35; TNF 9475; NOS 63; TNE 367330; TFE 265564

EPA GENETOX PROGRAM 1988, NEGATIVE: CHO GENE MUTATION

EPA TSCA SECTION 8(B) CHEMICAL INVENTORY

EPA TSCA SECTION 8(D) UNPUBLISHED HEALTH/SAFETY STUDIES

EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, SEPTEMBER 1999

NIOSH CURRENT INTELLIGENCE BULLETIN #55, SEPTEMBER 1991

NIOSH ANALYTICAL METHOD, 1994: GLUTARALDEHYDE, 2532

NTP TOXICITY STUDIES, RPT# TOX-25, SEPTEMBER 1999

NTP CARCINOGENESIS STUDIES; TEST COMPLETED (CAMERA COPY IN PROGRESS),

SEPTEMBER 1999

OSHA ANALYTICAL METHOD #ID-64

SECTION 16. - - - - - - - - - - OTHER INFORMATION- - - - - - - - - - - -

THE ABOVE INFORMATION IS BELIEVED TO BE CORRECT BUT DOES NOT PURPORT TO

BE ALL INCLUSIVE AND SHALL BE USED ONLY AS A GUIDE. SIGMA, ALDRICH,

FLUKA SHALL NOT BE HELD LIABLE FOR ANY DAMAGE RESULTING FROM HANDLING

OR FROM CONTACT WITH THE ABOVE PRODUCT. SEE REVERSE SIDE OF INVOICE OR

PACKING SLIP FOR ADDITIONAL TERMS AND CONDITIONS OF SALE.

COPYRIGHT 1999 SIGMA-ALDRICH CO.

LICENSE GRANTED TO MAKE UNLIMITED PAPER COPIES FOR INTERNAL USE ONLY

SECTION 1. - - - - - - - - - CHEMICAL IDENTIFICATION- - - - - - - - - -

CATALOG #: H7020

NAME: HYDROCHLORIC ACID ACS REAGENT

SECTION 2. - - - - - COMPOSITION/INFORMATION ON INGREDIENTS - - - - - -

CAS #: 7647-01-0

MF: HCL

EC NO: 231-595-7

SYNONYMS

ACIDE CHLORHYDRIQUE (FRENCH) * ACIDO CLORIDRICO (ITALIAN) * ANHYDROUS

HYDROCHLORIC ACID * CHLOORWATERSTOF (DUTCH) * CHLOROHYDRIC ACID *

CHLOROWODOR (POLISH) * CHLORWASSERSTOFF (GERMAN) * HYDROCHLORIDE *

HYDROGEN CHLORIDE (ACGIH:OSHA) * MURIATIC ACID * SPIRITS OF SALT *

SECTION 3. - - - - - - - - - - HAZARDS IDENTIFICATION - - - - - - - - -

LABEL PRECAUTIONARY STATEMENTS

CORROSIVE

CAUSES BURNS.

TOXIC BY INHALATION AND IF SWALLOWED.

REACTS VIOLENTLY WITH WATER.

IN CASE OF CONTACT WITH EYES, RINSE IMMEDIATELY WITH PLENTY OF

WATER AND SEEK MEDICAL ADVICE.

TAKE OFF IMMEDIATELY ALL CONTAMINATED CLOTHING.

WEAR SUITABLE PROTECTIVE CLOTHING, GLOVES AND EYE/FACE

PROTECTION.

DO NOT BREATHE VAPOR.

SECTION 4. - - - - - - - - - - FIRST-AID MEASURES- - - - - - - - - - -

IF SWALLOWED, WASH OUT MOUTH WITH WATER PROVIDED PERSON IS CONSCIOUS.

CALL A PHYSICIAN IMMEDIATELY.

IN CASE OF CONTACT, IMMEDIATELY FLUSH EYES OR SKIN WITH COPIOUS

AMOUNTS OF WATER FOR AT LEAST 15 MINUTES WHILE REMOVING CONTAMINATED

CLOTHING AND SHOES.

IF INHALED, REMOVE TO FRESH AIR. IF NOT BREATHING GIVE ARTIFICIAL

RESPIRATION. IF BREATHING IS DIFFICULT, GIVE OXYGEN.

ASSURE ADEQUATE FLUSHING OF THE EYES BY SEPARATING THE EYELIDS

WITH FINGERS.

SECTION 5. - - - - - - - - - FIRE FIGHTING MEASURES - - - - - - - - - -

EXTINGUISHING MEDIA

NONCOMBUSTIBLE.

USE EXTINGUISHING MEDIA APPROPRIATE TO SURROUNDING FIRE CONDITIONS.

SPECIAL FIREFIGHTING PROCEDURES

WEAR SELF-CONTAINED BREATHING APPARATUS AND PROTECTIVE CLOTHING TO

PREVENT CONTACT WITH SKIN AND EYES.

USE WATER SPRAY TO COOL FIRE-EXPOSED CONTAINERS.

UNUSUAL FIRE AND EXPLOSIONS HAZARDS

EMITS TOXIC FUMES UNDER FIRE CONDITIONS.

SECTION 6. - - - - - - - - ACCIDENTAL RELEASE MEASURES- - - - - - - - -

EVACUATE AREA.

WEAR SELF-CONTAINED BREATHING APPARATUS, RUBBER BOOTS AND HEAVY

RUBBER GLOVES.

COVER WITH DRY-LIME, SAND, OR SODA ASH. PLACE IN COVERED CONTAINERS

USING NON-SPARKING TOOLS AND TRANSPORT OUTDOORS.

SECTION 7. - - - - - - - - - - HANDLING AND STORAGE- - - - - - - - - - -

REFER TO SECTION 8.

SECTION 8. - - - - - - EXPOSURE CONTROLS/PERSONAL PROTECTION- - - - - -

FACESHIELD (8-INCH MINIMUM).

WASH CONTAMINATED CLOTHING BEFORE REUSE.

DISCARD CONTAMINATED SHOES.

WASH THOROUGHLY AFTER HANDLING.

NIOSH/MSHA-APPROVED RESPIRATOR IN NONVENTILATED AREAS AND/OR FOR

EXPOSURE ABOVE THE ACGIH TLV.

RUBBER GLOVES.

CHEMICAL SAFETY GOGGLES.

SAFETY SHOWER AND EYE BATH.

AVOID BREATHING VAPOR.

DO NOT GET IN EYES, ON SKIN, ON CLOTHING.

AVOID PROLONGED OR REPEATED EXPOSURE.

KEEP TIGHTLY CLOSED.

STORE IN A COOL DRY PLACE.

REACTS VIOLENTLY WITH WATER.

MAY DEVELOP PRESSURE.

SECTION 9. - - - - - - - PHYSICAL AND CHEMICAL PROPERTIES - - - - - - -

APPEARANCE AND ODOR

LIQUID.

PHYSICAL PROPERTIES

VAPOR PRESSURE: 409,981 MMHG

SPECIFIC GRAVITY: 1,19

VAPOR DENSITY: 1,3 G/L

SECTION 10. - - - - - - - - -STABILITY AND REACTIVITY - - - - - - - - -

INCOMPATIBILITIES

DO NOT ALLOW WATER TO ENTER CONTAINER BECAUSE OF VIOLENT REACTION.

AMINES

ALKALI METALS

COPPER, COPPER ALLOYS

HAZARDOUS COMBUSTION OR DECOMPOSITION PRODUCTS

HYDROGEN CHLORIDE GAS

SECTION 11. - - - - - - - - - TOXICOLOGICAL INFORMATION - - - - - - - -

ACUTE EFFECTS

MAY BE FATAL IF INHALED, SWALLOWED, OR ABSORBED THROUGH SKIN.

CAUSES BURNS.

MATERIAL IS EXTREMELY DESTRUCTIVE TO TISSUE OF THE MUCOUS MEMBRANES

AND UPPER RESPIRATORY TRACT, EYES AND SKIN.

INHALATION MAY RESULT IN SPASM, INFLAMMATION AND EDEMA OF THE

LARYNX AND BRONCHI, CHEMICAL PNEUMONITIS AND PULMONARY EDEMA.

SYMPTOMS OF EXPOSURE MAY INCLUDE BURNING SENSATION, COUGHING,

WHEEZING, LARYNGITIS, SHORTNESS OF BREATH, HEADACHE, NAUSEA AND

VOMITING.

RTECS #: MW4025000

HYDROCHLORIC ACID

IRRITATION DATA

EYE-RBT 5 MG/30S RINSE MLD TXCYAC 23,281,1982

TOXICITY DATA

ORL-MAN LDLO:2857 UG/KG MJAUAJ 158,28,1993

ORL-WMN LDLO:420 UL/KG JJTOEX 9,351,1996

IHL-HMN LCLO:1300 PPM/30M 29ZWAE -,207,1968

IHL-HMN LCLO:3000 PPM/5M TABIA2 3,231,1933

UNR-MAN LDLO:81 MG/KG 85DCAI 2,73,1970

IHL-RAT LC50:3124 PPM/1H AMRL** TR-74-78,1974

IHL-MUS LC50:1108 PPM/1H JCTODH 3,61,1976

IPR-MUS LD50:40142 UG/KG COREAF 256,1043,1963

ORL-RBT LD50:900 MG/KG BIZEA2 134,437,1923

TARGET ORGAN DATA

SENSE ORGANS AND SPECIAL SENSES (OTHER EYE EFFECTS)

VASCULAR (BP LOWERING NOT CHARACTERIZED IN AUTONOMIC SECTION)

LUNGS, THORAX OR RESPIRATION (RESPIRATORY DEPRESSION)

LUNGS, THORAX OR RESPIRATION (RESPIRATORY STIMULATION)

GASTROINTESTINAL (CHANGES IN STRUCTURE OR FUNCTION OF ESOPHAGUS)

SKIN AND APPENDAGES (AFTER SYSTEMIC EXPOSURE: DERMATITIS, OTHER)

ONLY SELECTED REGISTRY OF TOXIC EFFECTS OF CHEMICAL SUBSTANCES

(RTECS) DATA IS PRESENTED HERE. SEE ACTUAL ENTRY IN RTECS FOR

COMPLETE INFORMATION.

SECTION 12. - - - - - - - - - ECOLOGICAL INFORMATION - - - - - - - - - -

DATA NOT YET AVAILABLE.

SECTION 13. - - - - - - - - - DISPOSAL CONSIDERATIONS - - - - - - - - -

CONTACT A LICENSED PROFESSIONAL WASTE DISPOSAL SERVICE TO DISPOSE OF

THIS MATERIAL.

OBSERVE ALL FEDERAL, STATE AND LOCAL ENVIRONMENTAL REGULATIONS.

SECTION 14. - - - - - - - - - - TRANSPORT INFORMATION - - - - - - - - -

CONTACT SIGMA CHEMICAL COMPANY FOR TRANSPORTATION INFORMATION.

SECTION 15. - - - - - - - - - REGULATORY INFORMATION - - - - - - - - - -

EUROPEAN INFORMATION

EC INDEX NO: 017-002-00-2

CORROSIVE

R 23

TOXIC BY INHALATION.

R 35

CAUSES SEVERE BURNS.

S 9

KEEP CONTAINER IN A WELL-VENTILATED PLACE.

S 26

IN CASE OF CONTACT WITH EYES, RINSE IMMEDIATELY WITH PLENTY OF

WATER AND SEEK MEDICAL ADVICE.

S 36/37/39

WEAR SUITABLE PROTECTIVE CLOTHING, GLOVES AND EYE/FACE

PROTECTION.

S 45

IN CASE OF ACCIDENT OR IF YOU FEEL UNWELL, SEEK MEDICAL ADVICE

IMMEDIATELY (SHOW THE LABEL WHERE POSSIBLE).

REVIEWS, STANDARDS, AND REGULATIONS

OEL=MAK

ACGIH TLV-CL 7.5 MG/M3 (5 PPM) DTLVS* TLV/BEI,1997

IARC CANCER REVIEW:HUMAN INADEQUATE EVIDENCE IMEMDT 54,189,1992

IARC CANCER REVIEW:ANIMAL INADEQUATE EVIDENCE IMEMDT 54,189,1992

IARC CANCER REVIEW:GROUP 3 IMEMDT 54,189,1992

EPA FIFRA 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTRATION

FEREAC 54,7740,1989

MSHA STANDARD:AIR-CL 5 PPM (7 MG/M3)

DTLVS* 3,129,1971

OSHA PEL (GEN INDU):CL 5 PPM (7 MG/M3)

CFRGBR 29,1910.1000,1994

OSHA PEL (CONSTRUC):CL 5 PPM (7 MG/M3)

CFRGBR 29,1926.55,1994

OSHA PEL (SHIPYARD):CL 5 PPM (7 MG/M3)

CFRGBR 29,1915.1000,1993

OSHA PEL (FED CONT):CL 5 PPM (7 MG/M3)

CFRGBR 41,50-204.50,1994

OEL-AUSTRALIA:TWA 5 PPM (7 MG/M3) JAN 1993

OEL-AUSTRIA: MAK 5 PPM (7 MG/M3), JAN1999

OEL-AUSTRIA:TWA 5 PPM (7 MG/M3) JAN 1993

OEL-BELGIUM:STEL 5 PPM (7.7 MG/M3) JAN 1993

OEL-DENMARK: TWA 5 PPM (7 MG/M3), JAN1999

OEL-FINLAND:STEL 5 PPM (7 MG/M3);SKIN JAN 1993

OEL-JAPAN: STEL 5 PPM (7.5 MG/M3), JAN1999

OEL-GERMANY:TWA 5 PPM (7 MG/M3) JAN 1993

OEL-HUNGARY:STEL 5 MG/M3 JAN 1993

OEL-JAPAN:STEL 5 PPM (7.5 MG/M3) JAN 1993

OEL-POLAND: MAC(TWA) 5 MG/M3, MAC(C) 7 MG/M3, JAN1999

OEL-THE NETHERLANDS:TWA 5 PPM (7 MG/M3) JAN 1993

OEL-THE PHILIPPINES:TWA 5 PPM (7 MG/M3) JAN 1993

OEL-RUSSIA:STEL 5 PPM (5 MG/M3) JAN 1993

OEL-SWEDEN:STEL 5 PPM (8 MG/M3) JAN 1993

OEL-SWITZERLAND:TWA 5 PPM (7.5 MG/M3);STEL 10 PPM (15 MG/M3) JAN 1993

OEL-THAILAND:TWA 5 PPM (7 MG/M3) JAN 1993

OEL-TURKEY:TWA 5 PPM (7 MG/M3) JAN 1993

OEL-UNITED KINGDOM:TWA 5 PPM (7 MG/M3);STEL 5 PPM (7 MG/M3) JAN 1993

OEL IN BULGARIA, COLOMBIA, JORDAN, KOREA CHECK ACGIH TLV

OEL IN NEW ZEALAND, SINGAPORE, VIETNAM CHECK ACGIH TLV

NIOSH REL TO HYDROGEN CHLORIDE-AIR:CL 5 PPM

NIOSH* DHHS #92-100,1992

NOHS 1974: HZD 38580; NIS 360; TNF 87434; NOS 156; TNE 824985

NOES 1983: HZD 38580; NIS 321; TNF 60309; NOS 183; TNE 1238572; TFE

388130

EPA GENETOX PROGRAM 1988, NEGATIVE: CELL TRANSFORM.-SA7/SHE

EPA TSCA SECTION 8(B) CHEMICAL INVENTORY

EPA TSCA SECTION 8(D) UNPUBLISHED HEALTH/SAFETY STUDIES

EPA TSCA SECTION 8(E) RISK NOTIFICATION, 8EHQ-0892-9246

ON EPA IRIS DATABASE

EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, SEPTEMBER 1999

NIOSH ANALYTICAL METHOD, 1994: ACIDS, INORGANIC, 7903

U.S. INFORMATION

THIS PRODUCT IS SUBJECT TO SARA SECTION 313 REPORTING REQUIREMENTS.

SECTION 16. - - - - - - - - - - OTHER INFORMATION- - - - - - - - - - - -

THE ABOVE INFORMATION IS BELIEVED TO BE CORRECT BUT DOES NOT PURPORT TO

BE ALL INCLUSIVE AND SHALL BE USED ONLY AS A GUIDE. SIGMA, ALDRICH,

FLUKA SHALL NOT BE HELD LIABLE FOR ANY DAMAGE RESULTING FROM HANDLING

OR FROM CONTACT WITH THE ABOVE PRODUCT. SEE REVERSE SIDE OF INVOICE OR

PACKING SLIP FOR ADDITIONAL TERMS AND CONDITIONS OF SALE.

COPYRIGHT 1999 SIGMA-ALDRICH CO.

LICENSE GRANTED TO MAKE UNLIMITED PAPER COPIES FOR INTERNAL USE ONLY

SECTION 1. - - - - - - - - - CHEMICAL IDENTIFICATION- - - - - - - - - -

CATALOG #: M3641

NAME: METHANOL SPECTROPHOTOMETRIC GRADE

SECTION 2. - - - - - COMPOSITION/INFORMATION ON INGREDIENTS - - - - - -

CAS #: 67-56-1

MF: CH4O

EC NO: 200-659-6

SYNONYMS

ALCOOL METHYLIQUE (FRENCH) * ALCOOL METILICO (ITALIAN) * BIELESKI'S

SOLUTION * CARBINOL * COLONIAL SPIRIT * COLUMBIAN SPIRIT * METANOLO

(ITALIAN) * METHANOL (ACGIH) * METHYL ALCOHOL (DOT:OSHA) * METHYLOL *

METHYLALKOHOL (GERMAN) * METHYL HYDRATE * METHYL HYDROXIDE * METYLOWY

ALKOHOL (POLISH) * MONOHYDROXYMETHANE * PYROXYLIC SPIRIT * RCRA WASTE

NUMBER U154 * WOOD ALCOHOL * WOOD NAPHTHA * WOOD SPIRIT *

SECTION 3. - - - - - - - - - - HAZARDS IDENTIFICATION - - - - - - - - -

LABEL PRECAUTIONARY STATEMENTS

FLAMMABLE (USA)

HIGHLY FLAMMABLE (EU)

TOXIC

TOXIC BY INHALATION AND IF SWALLOWED.

IRRITATING TO EYES AND SKIN.

TARGET ORGAN(S):

EYES

KIDNEYS

KEEP CONTAINER TIGHTLY CLOSED.

KEEP AWAY FROM SOURCES OF IGNITION - NO SMOKING.

AVOID CONTACT WITH SKIN.

IN CASE OF ACCIDENT OR IF YOU FEEL UNWELL, SEEK MEDICAL ADVICE

IMMEDIATELY (SHOW THE LABEL WHERE POSSIBLE).

TAKE PRECAUTIONARY MEASURES AGAINST STATIC DISCHARGES.

SECTION 4. - - - - - - - - - - FIRST-AID MEASURES- - - - - - - - - - -

IF SWALLOWED, WASH OUT MOUTH WITH WATER PROVIDED PERSON IS CONSCIOUS.

CALL A PHYSICIAN IMMEDIATELY.

IF INHALED, REMOVE TO FRESH AIR. IF NOT BREATHING GIVE ARTIFICIAL

RESPIRATION. IF BREATHING IS DIFFICULT, GIVE OXYGEN.

IN CASE OF SKIN CONTACT, FLUSH WITH COPIOUS AMOUNTS OF WATER

FOR AT LEAST 15 MINUTES. REMOVE CONTAMINATED CLOTHING AND

SHOES. CALL A PHYSICIAN.

IN CASE OF CONTACT WITH EYES, FLUSH WITH COPIOUS AMOUNTS OF WATER

FOR AT LEAST 15 MINUTES. ASSURE ADEQUATE FLUSHING BY SEPARATING

THE EYELIDS WITH FINGERS. CALL A PHYSICIAN.

SECTION 5. - - - - - - - - - FIRE FIGHTING MEASURES - - - - - - - - - -

EXTINGUISHING MEDIA

WATER SPRAY.

CARBON DIOXIDE, DRY CHEMICAL POWDER OR APPROPRIATE FOAM.

SPECIAL FIREFIGHTING PROCEDURES

WEAR SELF-CONTAINED BREATHING APPARATUS AND PROTECTIVE CLOTHING TO

PREVENT CONTACT WITH SKIN AND EYES.

UNUSUAL FIRE AND EXPLOSIONS HAZARDS

COMBUSTIBLE LIQUID.

SECTION 6. - - - - - - - - ACCIDENTAL RELEASE MEASURES- - - - - - - - -

WEAR RESPIRATOR, CHEMICAL SAFETY GOGGLES, RUBBER BOOTS AND HEAVY

RUBBER GLOVES.

COVER WITH DRY-LIME, SAND, OR SODA ASH. PLACE IN COVERED CONTAINERS

USING NON-SPARKING TOOLS AND TRANSPORT OUTDOORS.

VENTILATE AREA AND WASH SPILL SITE AFTER MATERIAL PICKUP IS COMPLETE.

SECTION 7. - - - - - - - - - - HANDLING AND STORAGE- - - - - - - - - - -

REFER TO SECTION 8.

SECTION 8. - - - - - - EXPOSURE CONTROLS/PERSONAL PROTECTION- - - - - -

SAFETY SHOWER AND EYE BATH.

MECHANICAL EXHAUST REQUIRED.

WASH THOROUGHLY AFTER HANDLING.

DO NOT BREATHE VAPOR.

AVOID CONTACT WITH EYES, SKIN AND CLOTHING.

AVOID PROLONGED OR REPEATED EXPOSURE.

WEAR APPROPRIATE NIOSH/MSHA-APPROVED RESPIRATOR, CHEMICAL-RESISTANT

GLOVES, SAFETY GOGGLES, OTHER PROTECTIVE CLOTHING.

KEEP CONTAINER CLOSED.

KEEP AWAY FROM HEAT AND OPEN FLAME.

STORE IN A COOL DRY PLACE.

ACIDS

ACID CHLORIDES

ACID ANHYDRIDES

OXIDIZING AGENTS

ALKALI METALS

REDUCING AGENTS

SECTION 9. - - - - - - - PHYSICAL AND CHEMICAL PROPERTIES - - - - - - -

APPEARANCE AND ODOR

LIQUID.

PHYSICAL PROPERTIES

BOILING POINT: 64.6 - 64.7 C

MELTING POINT: -98 C

FLASHPOINT 52 F

11 C

EXPLOSION LIMITS IN AIR:

UPPER 36 %

LOWER 6 %

VAPOR PRESSURE: 410 MMHG

SPECIFIC GRAVITY: 0.791

VAPOR DENSITY: 1.1 G/L

SECTION 10. - - - - - - - - -STABILITY AND REACTIVITY - - - - - - - - -

STABILITY

STABLE.

HAZARDOUS COMBUSTION OR DECOMPOSITION PRODUCTS

CARBON MONOXIDE, CARBON DIOXIDE

NITROGEN OXIDES

HYDROGEN CHLORIDE GAS

HAZARDOUS POLYMERIZATION

WILL NOT OCCUR.

SECTION 11. - - - - - - - - - TOXICOLOGICAL INFORMATION - - - - - - - -

ACUTE EFFECTS

CAUSES SKIN IRRITATION.

MAY BE HARMFUL IF ABSORBED THROUGH THE SKIN.

CAUSES EYE IRRITATION.

HARMFUL IF INHALED.

MATERIAL MAY BE IRRITATING TO MUCOUS MEMBRANES AND UPPER

RESPIRATORY TRACT.

HARMFUL IF SWALLOWED.

GASTROINTESTINAL DISTURBANCES

MAY CAUSE CONVULSIONS.

TO THE BEST OF OUR KNOWLEDGE, THE CHEMICAL, PHYSICAL, AND

TOXICOLOGICAL PROPERTIES HAVE NOT BEEN THOROUGHLY INVESTIGATED.

CHRONIC EFFECTS

TARGET ORGAN(S):

EYES

KIDNEYS

LIVER

HEART

RTECS #: PC1400000

METHANOL

IRRITATION DATA

SKN-RBT 20 MG/24H MOD 85JCAE -,187,1986

EYE-RBT 40 MG MOD UCDS** 3/24/1970

EYE-RBT 100 MG/24H MOD 85JCAE -,187,1986

TOXICITY DATA

ORL-MAN LDLO:6422 MG/KG CMAJAX 128,14,1983

ORL-HMN LDLO:428 MG/KG NPIRI* 1,74,1974

ORL-HMN LDLO:143 MG/KG 34ZIAG -,382,1969

UNR-MAN LDLO:868 MG/KG 85DCAI 2,73,1970

ORL-RAT LD50:5628 MG/KG GTPZAB 19(11),27,1975

IHL-RAT LC50:64000 PPM/4H NPIRI* 1,74,1974

IPR-RAT LD50:7529 MG/KG EVHPAZ 61,321,1985

IVN-RAT LD50:2131 MG/KG EVHPAZ 61,321,1985

ORL-MUS LD50:7300 MG/KG TXCYAC 25,271,1982

IPR-MUS LD50:10765 MG/KG EVHPAZ 61,321,1985

SCU-MUS LD50:9800 MG/KG TXAPA9 18,185,1971

IVN-MUS LD50:4710 MG/KG EVHPAZ 61,321,1985

ORL-MKY LD50:7 GM/KG TXAPA9 3,202,1961

ORL-RBT LD50:14200 MG/KG FAONAU 48A,105,1970

SKN-RBT LD50:15800 MG/KG NPIRI* 1,74,1974

IPR-RBT LD50:1826 MG/KG EVHPAZ 61,321,1985

IVN-RBT LD50:8907 MG/KG EVHPAZ 61,321,1985

IPR-GPG LD50:3556 MG/KG EVHPAZ 61,321,1985

IPR-HAM LD50:8555 MG/KG EVHPAZ 61,321,1985

TARGET ORGAN DATA

SENSE ORGANS AND SPECIAL SENSES (OPTIC NERVE NEUROPATHY)

SENSE ORGANS AND SPECIAL SENSES (VISUAL FIELD CHANGES)

BEHAVIORAL (HEADACHE)

LUNGS, THORAX OR RESPIRATION (DYSPNAE)

LUNGS, THORAX OR RESPIRATION (OTHER CHANGES)

GASTROINTESTINAL (NAUSEA OR VOMITING)

SPECIFIC DEVELOPMENTAL ABNORMALITIES (CENTRAL NERVOUS SYSTEM)

SPECIFIC DEVELOPMENTAL ABNORMALITIES (MUSCULOSKELETAL SYSTEM)

ONLY SELECTED REGISTRY OF TOXIC EFFECTS OF CHEMICAL SUBSTANCES

(RTECS) DATA IS PRESENTED HERE. SEE ACTUAL ENTRY IN RTECS FOR

COMPLETE INFORMATION.

SECTION 12. - - - - - - - - - ECOLOGICAL INFORMATION - - - - - - - - - -

DATA NOT YET AVAILABLE.

SECTION 13. - - - - - - - - - DISPOSAL CONSIDERATIONS - - - - - - - - -

BURN IN A CHEMICAL INCINERATOR EQUIPPED WITH AN AFTERBURNER AND

SCRUBBER BUT EXERT EXTRA CARE IN IGNITING AS THIS MATERIAL IS HIGHLY

FLAMMABLE.

OBSERVE ALL FEDERAL, STATE AND LOCAL ENVIRONMENTAL REGULATIONS.

SECTION 14. - - - - - - - - - - TRANSPORT INFORMATION - - - - - - - - -

CONTACT SIGMA CHEMICAL COMPANY FOR TRANSPORTATION INFORMATION.

SECTION 15. - - - - - - - - - REGULATORY INFORMATION - - - - - - - - - -

EUROPEAN INFORMATION

EC INDEX NO: 603-001-00-X

HIGHLY FLAMMABLE

TOXIC

R 11

HIGHLY FLAMMABLE.

R 23/25

TOXIC BY INHALATION AND IF SWALLOWED.

S 7

KEEP CONTAINER TIGHTLY CLOSED.

S 16

KEEP AWAY FROM SOURCES OF IGNITION - NO SMOKING.

S 24

AVOID CONTACT WITH SKIN.

S 45

IN CASE OF ACCIDENT OR IF YOU FEEL UNWELL, SEEK MEDICAL ADVICE

IMMEDIATELY (SHOW THE LABEL WHERE POSSIBLE).

REVIEWS, STANDARDS, AND REGULATIONS

OEL=MAK

ACGIH TLV-STEL 328 MG/M3 (250 PPM) (SKIN) DTLVS* TLV/BEI,1997

ACGIH TLV-TWA 262 MG/M3 (200 PPM) DTLVS* TLV/BEI,1997

EPA FIFRA 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTRATION

FEREAC 54,7740,1989

MSHA STANDARD-AIR:TWA 200 PPM (260 MG/M3) (SKIN)

DTLVS* 3,155,1971

OSHA PEL (GEN INDU):8H TWA 200 PPM (260 MG/M3)

CFRGBR 29,1910.1000,1994

OSHA PEL (CONSTRUC):8H TWA 200 PPM (260 MG/M3)

CFRGBR 29,1926.55,1994

OSHA PEL (SHIPYARD):8H TWA 200 PPM (260 MG/M3)

CFRGBR 29,1915.1000,1993

OSHA PEL (FED CONT):8H TWA 200 PPM (260 MG/M3)

CFRGBR 41,50-204.50,1994

OEL-ARAB REPUBLIC OF EGYPT:TWA 200 PPM (260 MG/M3);SKIN JAN 1993

OEL-AUSTRIA: MAK 200 PPM (260 MG/M3), SKIN, JAN1999

OEL-AUSTRALIA:TWA 200 PPM (260 MG/M3);STEL 250 PPM;SKIN JAN 1993

OEL-BELGIUM:TWA 200 PPM (262 MG/M3);STEL 250 PPM;SKIN JAN 1993

OEL-DENMARK: TWA 200 PPM (260 MG/M3), SKIN, JAN1999

OEL-FINLAND:TWA 200 PPM (260 MG/M3);STEL 250 PPM;SKIN JAN 1993

OEL-JAPAN: OEL 200 PPM (260 MG/M3), SKIN, JAN1999

OEL-GERMANY:TWA 200 PPM (260 MG/M3);SKIN JAN 1993

OEL-HUNGARY:TWA 50 MG/M3;STEL 100 MG/M3;SKIN JAN 1993

OEL-POLAND: MAC(TWA) 100 MG/M3, MAC(STEL) 300 MG/M3, JAN1999

OEL-JAPAN:TWA 200 PPM (260 MG/M3);SKIN JAN 1993

OEL-THE NETHERLANDS:TWA 200 PPM (260 MG/M3);SKIN JAN 1993

OEL-THE PHILIPPINES:TWA 200 PPM (260 MG/M3) JAN 1993

OEL-RUSSIA:TWA 200 PPM;STEL 5 MG/M3;SKIN JAN 1993

OEL-SWEDEN:TWA 200 PPM (250 MG/M3);STEL 250 PPM (350 MG/M3);SKIN JAN

1993

OEL-SWITZERLAND:TWA 200 PPM (260 MG/M3);STEL 400 PPM;SKIN JAN 1993

OEL-THAILAND:TWA 200 PPM (260 MG/M3) JAN 1993

OEL-TURKEY:TWA 200 PPM (260 MG/M3) JAN 1993

OEL-UNITED KINGDOM:TWA 200 PPM (260 MG/M3);STEL 250 PPM;SKIN JAN 1993

OEL IN BULGARIA, COLOMBIA, JORDAN, KOREA CHECK ACGIH TLV

OEL IN NEW ZEALAND, SINGAPORE, VIETNAM CHECK ACGIH TLV

NIOSH REL TO METHANOL-AIR:10H TWA 200 PPM (SK);STEL 250 PPM (SK)

NIOSH* DHHS #92-100,1992

NOHS 1974: HZD 45930; NIS 344; TNF 78840; NOS 203; TNE 737242

NOES 1983: HZD 45930; NIS 373; TNF 101075; NOS 225; TNE 1620617; TFE

388352

EPA GENETOX PROGRAM 1988, NEGATIVE: SHE-CLONAL ASSAY; CELL

TRANSFORM.-SA7/SHE

EPA GENETOX PROGRAM 1988, NEGATIVE: N CRASSA-ANEUPLOIDY; IN VITRO

SCE-NONHUMAN

EPA TSCA SECTION 8(B) CHEMICAL INVENTORY

EPA TSCA SECTION 8(D) UNPUBLISHED HEALTH/SAFETY STUDIES

EPA TSCA SECTION 8(E) RISK NOTIFICATION, 8EHQ-0892-8989

ON EPA IRIS DATABASE

EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, SEPTEMBER 1999

NIOSH ANALYTICAL METHOD, 1994: METHANOL, 2000

NIOSH ANALYTICAL METHOD, 1996: VOLATILE ORGANIC COMPOUND, 2549

U.S. INFORMATION

THIS PRODUCT IS SUBJECT TO SARA SECTION 313 REPORTING REQUIREMENTS.

SECTION 16. - - - - - - - - - - OTHER INFORMATION- - - - - - - - - - - -

THE ABOVE INFORMATION IS BELIEVED TO BE CORRECT BUT DOES NOT PURPORT TO

BE ALL INCLUSIVE AND SHALL BE USED ONLY AS A GUIDE. SIGMA, ALDRICH,

FLUKA SHALL NOT BE HELD LIABLE FOR ANY DAMAGE RESULTING FROM HANDLING

OR FROM CONTACT WITH THE ABOVE PRODUCT. SEE REVERSE SIDE OF INVOICE OR

PACKING SLIP FOR ADDITIONAL TERMS AND CONDITIONS OF SALE.

COPYRIGHT 1999 SIGMA-ALDRICH CO.

LICENSE GRANTED TO MAKE UNLIMITED PAPER COPIES FOR INTERNAL USE ONLY