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Treponema denticola Search Results

Record: 1 of 1  
MiniMap IGR1408 IGR1405 IGR1407 IGR1406 IGR1404 apt, - TDE1684 ccmA5, - TDE1689 aroA, - TDE1686 TDE1688 atpA,fliI',ntpA, - TDE1683 TDE1687 TDE1685 apt, - TDE1684 ccmA5, - TDE1689 aroA, - TDE1686 TDE1688 atpA,fliI',ntpA, - TDE1683 TDE1687 TDE1685 apt, - TDE1684 ccmA5, - TDE1689 aroA, - TDE1686 TDE1688 atpA,fliI',ntpA, - TDE1683 TDE1687 TDE1685


Gene ID:TDE1686

DNA Molecule Name:
1  

GenBank ID:
42527192

Gene Name:
aroA  

Definition:
3-phosphoshikimate-1-carboxyvinyltransferase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
1741853

Gene Stop:
1740507

Gene Length:
1347

Molecular Weight*:
49166

pI*:


Net Charge*:


EC:
2.5.1.19  

Functional Class:
Amino acid biosynthesis; Aromatic amino acid family  

Gene Ontology:

Pathway: pathway table
Phenylalanine, tyrosine and tryptophan biosynthesis

Secondary Evidence:
Garside LH, Collins M, Langford PR, Rycroft AN.
Actinobacillus pleuropneumoniae serotype 1 carrying the defined aroA mutation is fully avirulent in the pig.
Res Vet Sci. 2002 Apr;72(2):163-7.
PMID: 12027598

Roberts M, Chatfield S, Pickard D, Li J, Bacon A.
Comparison of abilities of Salmonella enterica serovar typhimurium aroA aroD and aroA htrA mutants to act as live vectors.
Infect Immun. 2000 Oct;68(10):6041-3.
PMID: 10992518

Comment:
Protein aroE is TDE1640, aroH is TDE1502, aroC is TDE1132, aroB is TDE0104, both aroF and aroG correspond to TDE2569 (3e-84), and aroK is TDE2447. TDE1640 also corresponds slightly to the previously reported sequence for aroD in GenBank (3e-05), and TDE2447 shows some correlation to the sequence for aroI in GenBank (4e-06). No genes correspond to the previously reported sequence for aroQ.

View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search

TDE1686 has no significant similarity (BLAST p-value < 1e-03) to a Treponema pallidum gene.

TDE1686 has no significant similarity (BLAST p-value < 1e-3) to a Borrelia burgdorferi gene.


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|42527192|ref|NP_972290.1|  3-phosphoshikimate 1-carboxyvi...   843   0.0  
 gi|46446516|ref|YP_007881.1|  putative 3-phosphoshikimate 1-...   249   2e-64
 gi|118727959|ref|ZP_01576540.1|  3-phosphoshikimate 1-carbox...   233   2e-59
 gi|125975565|ref|YP_001039475.1|  3-phosphoshikimate 1-carbo...   229   2e-58
 gi|147920933|ref|YP_685260.1|  putative 3-phosphoshikimate 1...   210   1e-52
 gi|150383884|ref|ZP_01922596.1|  3-phosphoshikimate 1-carbox...   199   2e-49
 gi|150402378|ref|YP_001329672.1|  3-phosphoshikimate 1-carbo...   198   7e-49
 gi|29840480|ref|NP_829586.1|  3-phosphoshikimate 1-carboxyvi...   198   7e-49
 gi|150399272|ref|YP_001323039.1|  3-phosphoshikimate 1-carbo...   196   2e-48
 gi|27904782|ref|NP_777908.1|  3-phosphoshikimate 1-carboxyvi...   196   3e-48


COGS Summary:  COGS Search
BeTs to clades of COG0128
COG name: 5-enolpyruvylshikimate-3-phosphate synthase
Functional Class:  E
The phylogenetic pattern of COG0128 is aompkzyqvdrlbcefghsnuj-i--
Number of proteins in this genome belonging to this COG is


Blocks Summary:  Blocks Search

Significant hit ( 8.2e-64) to 9/9 blocks of the IPB001986 family, which is described as "EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)". Interpro entry for IPR001986.
    IPB001986A    14-54    1e-13
    IPB001986B    97-107    8.7
    IPB001986C    121-147    0.0093
    IPB001986D    181-193    64
    IPB001986E    208-224    0.0017
    IPB001986F    250-262    0.034
    IPB001986G    282-296    0.00035
    IPB001986H    331-383    3.3e-22
    IPB001986I    396-408    0.0028

ProDom Summary:  Protein Domain Search

Residues 2 to 446 match (4e-47) PD:PD001867 which is described as TRANSFERASE 1-CARBOXYVINYLTRANSFERASE SYNTHASE COMPLETE PROTEOME UDP-N-ACETYLGLUCOSAMINE 3-PHOSPHOSHIKIMATE EPSP BIOSYNTHESIS ACID

Paralogs:  Local Blast Search

TDE1686 has no significant similarity (blastp p-value < 1e-3) to any other gene in this genome.


Pfam Summary:  Pfam Search

Residues 7 to 440 (E-value = 1.1e-88) place TDE1686 in the EPSP_synthase family which is described as EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase) (PF00275)


Structural Feature(s):
Feature Type  Start  Stop
transmembrane  
104  
121
transmembrane  
252  
270
transmembrane  
327  
344

Top PDB Hits:

PDB hits to TDE1686 from Psi-BLAST round 5 vs. nr database

27.1% similar to PDB:1G6S Structure Of Epsp Synthase Liganded With Shikimate-3- Phosphate And Glyphosate (Chain A; 1e-111)
27.1% similar to PDB:1G6T Structure Of Epsp Synthase Liganded With Shikimate-3- Phosphate (Chain A; 1e-111)
26.9% similar to PDB:1MI4 Glyphosate Insensitive G96a Mutant Epsp Synthase Liganded With Shikimate-3-Phosp (Chain A; 1e-110)
26.9% similar to PDB:1EPS 5-Enol-Pyruvyl-3-Phosphate Synthase (E.C.2.5.1.9) (1e-110)
17.4% similar to PDB:1NAW Enolpyruvyl Transferase (Chain A,B; 1e-109)
17.4% similar to PDB:1EYN Structure Of Mura Liganded With The Extrinsic Fluorescence Probe Ans (Chain A; 1e-109)
17.4% similar to PDB:1EJC Crystal Structure Of Unliganded Mura (Type2) (Chain A; 1e-109)
17.4% similar to PDB:1EJD Crystal Structure Of Unliganded Mura (Type1) (Chain A,B; 1e-109)
17.6% similar to PDB:1DLG Crystal Structure Of The C115s Enterobacter Cloacae Mura In The Un-Liganded Stat (Chain A,B; 1e-108)
17.1% similar to PDB:1Q3G Mura (Asp305ala) Liganded With Tetrahedral Reaction Intermediate (Chain A,B,C,D,E,F,G,H,I,J,K,L,W,X,Y,Z; 1e-108)

Gene Protein Sequence:
MILKIKKSKPVSPMPIEVPPSKSHSMRALVLATFAEGPSQIKNLLMSGDT
KTAVSVFESLGVKFKIEQKNISSADIIVFPPKEGLKKRIEKQKQIKIDAG
NSGTLFYFLGSILSLMSSDFILTGDSSILKRPAKPLIEIYEELGLKYEFL
DGFEKAPIRVLPGSSSIKNLKGKTLCLEGDFSQVVSGLLLGAGLLDFPLQ
INLKRAGELPYLKMTLHWLKTCGIKFDVSDDFKTFKIIESQKILGFSAGI
PADWSSAAFPIALALITASTISIKNIDINDVQGDARIVKVLKEMNADIRF
EEKSQTLKIFPSSLKGGTFDCSDIPDAVPALSAIACFAKGETILKNIEIC
RYKECDRLSVISSELKKLGADITEGRDVLHIIGNAGKNLSPASVDSHKDH
RIAMMLTIIGFGIDDKDGTVFTLKNAECFDISYPSFLEDLKRMGFNISQ

Gene Nucleotide Sequence:  Sequence Viewer
ATGATTTTAAAGATTAAAAAATCAAAACCCGTATCTCCCATGCCCATTGA
AGTACCTCCTTCAAAGAGTCATTCAATGAGGGCTCTTGTTTTGGCTACAT
TTGCTGAAGGTCCTTCCCAAATAAAAAATCTTCTGATGAGCGGAGACACT
AAAACGGCTGTATCGGTATTTGAATCCTTAGGCGTAAAATTTAAAATAGA
ACAAAAAAACATATCCTCTGCCGATATAATTGTTTTTCCGCCGAAAGAAG
GCTTAAAAAAAAGAATCGAAAAACAAAAGCAGATAAAAATAGATGCGGGA
AATTCCGGCACTCTTTTTTACTTTTTAGGTTCCATTCTTTCTTTAATGTC
TTCCGATTTTATTTTAACCGGAGATTCATCTATTTTAAAGCGTCCCGCGA
AGCCTTTAATCGAAATTTATGAGGAGCTCGGTTTAAAATACGAATTTCTT
GACGGTTTTGAAAAGGCTCCCATACGGGTTTTACCCGGCTCATCTTCAAT
TAAAAACCTTAAAGGAAAAACGCTCTGTCTTGAGGGGGATTTTTCACAGG
TTGTAAGCGGCCTCCTGCTTGGAGCCGGCTTACTCGATTTTCCTTTACAA
ATAAATTTAAAAAGAGCAGGGGAGCTTCCCTACTTAAAAATGACCCTTCA
TTGGCTTAAAACTTGCGGTATTAAATTTGATGTTTCGGACGATTTTAAAA
CTTTTAAAATTATAGAAAGTCAAAAAATTTTAGGTTTTTCGGCAGGCATT
CCGGCCGACTGGTCTAGTGCTGCCTTTCCCATTGCCTTAGCCCTGATTAC
GGCTTCAACTATCAGCATAAAGAATATAGATATAAATGATGTTCAAGGAG
ATGCGAGGATAGTAAAGGTCTTAAAAGAGATGAATGCGGATATCCGCTTT
GAGGAGAAATCGCAAACCCTTAAAATTTTTCCTTCAAGTTTAAAGGGCGG
AACCTTTGACTGTTCCGATATTCCCGATGCAGTTCCGGCTCTTTCGGCGA
TAGCCTGTTTTGCAAAAGGCGAAACCATTTTAAAAAATATAGAAATCTGC
CGTTATAAAGAGTGTGACAGACTTTCCGTAATTTCCTCAGAATTAAAAAA
ACTTGGAGCCGATATTACGGAGGGAAGGGATGTTTTACATATAATAGGAA
ACGCAGGTAAAAACCTAAGCCCCGCCTCGGTTGATTCTCATAAAGATCAC
CGCATAGCTATGATGCTTACCATTATAGGCTTTGGTATTGATGATAAAGA
TGGGACTGTATTTACCCTAAAAAACGCAGAGTGTTTTGATATAAGTTATC
CATCCTTTTTGGAAGATCTAAAAAGGATGGGATTTAACATTAGTCAA

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