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DOI 10.1016/j.virol.2004.01.028
Title An avirulent chimeric Pestivirus with altered cell tropism protects pigs against lethal infection with classical swine fever virus
Creator/Author Reimann, Ilona ; Depner, Klaus ; Trapp, Sascha ; Beer, Martin E-mail: Martin.Beer@rie.bfav.de
Publication Date2004 Apr 25
OSTI IdentifierOSTI ID: 20495932
Other Number(s)Journal ID: ISSN 0042-6822; VIRLAX; TRN: US04R3542068164
Resource TypeJournal Article
Resource RelationJournal: Virology; Journal Volume: 322; Journal Issue: 1; Other Information: DOI: 10.1016/j.virol.2004.01.028; PII: S0042682204000911; Copyright (c) 2004 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); PBD: 25 Apr 2004
Subject60 APPLIED LIFE SCIENCES; ANTIBODIES; CATTLE; CELL CULTURES; DIARRHEA; ENZYME IMMUNOASSAY; FEVER; INOCULATION; RNA; SWINE; VACCINES; VIRAL DISEASES; VIRULENCE; VIRUSES
Description/Abstract A chimeric Pestivirus was constructed using an infectious cDNA clone of bovine viral diarrhea virus (BVDV) [J. Virol. 70 (1996) 8606]. After deletion of the envelope protein E2-encoding region, the respective sequence of classical swine fever virus (CSFV) strain Alfort 187 was inserted in-frame resulting in plasmid pA/CP7{sub E}2alf. After transfection of in vitro-transcribed CP7{sub E}2alf RNA, autonomous replication of chimeric RNA in bovine and porcine cell cultures was observed. Efficient growth of chimeric CP7{sub E}2alf virus, however, could only be demonstrated on porcine cells, and in contrast to the parental BVDV strain CP7, CP7{sub E}2alf only inefficiently infected and propagated in bovine cells. The virulence, immunogenicity, and 'marker vaccine' properties of the generated chimeric CP7{sub E}2alf virus were determined in an animal experiment using 27 pigs. After intramuscular inoculation of 1 x 10{sup 7} TCID{sub 50}, CP7{sub E}2alf proved to be completely avirulent, and neither viremia nor virus transmission to contact animals was observed; however, CSFV-specific neutralizing antibodies were detected from day 11 after inoculation. In addition, sera from all animals reacted positive in an E2-specific CSFV-antibody ELISA, but were negative for CSFV-E{sup RNS}-specific antibodies as determined with a CSFV marker ELISA. After challenge infection with highly virulent CSFV strain Eystrup, pigs immunized with CP7{sub E}2alf were fully protected against clinical signs of CSFV infection, viremia, and shedding of challenge virus, and almost all animals scored positive in a CSFV marker ELISA. From our results, we conclude that chimeric CP7{sub E}2alf may not only serve as a tool for a better understanding of Pestivirus attachment, entry, and assembly, but also represents an innocuous and efficacious modified live CSFV 'marker vaccine'.
Country of PublicationUnited States
LanguageEnglish
FormatSize: page(s) 143-157
System Entry Date2004 Jul 13

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