BLASTX+BEAUTY Search Results

WU-BLAST 2.0 search of the National Center for Biotechnology Information's NR Protein Database.

BEAUTY post-processing provided by the Human Genome Sequencing Center, Baylor College of Medicine.

BEAUTY Reference:
Worley KC, Culpepper P, Wiese BA, Smith RF. BEAUTY-X: enhanced BLAST searches for DNA queries. Bioinformatics 1998;14(10):890-1. Abstract

Worley KC, Wiese BA, Smith RF. BEAUTY: an enhanced BLAST-based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res 1995 Sep;5(2):173-84 Abstract



RepeatMasker repeats found in sequence:

No Repeats Found.

Reference:  Gish, Warren (1994-1997).  unpublished.
Gish, Warren and David J. States (1993).  Identification of protein coding
regions by database similarity search.  Nat. Genet. 3:266-72.

Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames.

Query= 'D07A06_A18_01.ab1' (465 letters)

  Translating both strands of query sequence in all 6 reading frames

Database: nr 625,274 sequences; 197,782,623 total letters.



     Observed Numbers of Database Sequences Satisfying
    Various EXPECTation Thresholds (E parameter values)

        Histogram units:      = 4 Sequences     : less than 4 sequences

 EXPECTation Threshold
 (E parameter)
    |
    V   Observed Counts-->
  10000 872 207 |===================================================
   6310 665 131 |================================
   3980 534 129 |================================
   2510 405 132 |=================================
   1580 273  86 |=====================
   1000 187  65 |================
    631 122  32 |========
    398  90  22 |=====
    251  68  21 |=====
    158  47  21 |=====
    100  26  10 |==
   63.1  16   1 |:
   39.8  15   3 |:
   25.1  12   0 |
   15.8  12   2 |:
 >>>>>>>>>>>>>>>>>>>>>  Expect = 10.0, Observed = 10  <<<<<<<<<<<<<<<<<
   10.0  10   0 |
   6.31  10   2 |:
   3.98   8   0 |
   2.51   8   2 |:


                                                                     Smallest
                                                                       Sum
                                                     Reading  High  Probability
Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N
gi|1173351|sp|P42552|S1FA_SPIOLDNA BINDING PROTEIN S1... +2   179  8.0e-13   1
gi|4371289|gb|AAD18147.1|(AC006260) unknown protein [... +2   168  1.2e-11   1
gi|1173348|sp|P42551|S1FA_ARATHDNA BINDING PROTEIN S1FA  +2   165  2.4e-11   1
gi|11357802|pir||T45877hypothetical protein F4P12.70 ... +2   165  2.4e-11   1
gi|1173349|sp|P42554|S1FA_MAIZEDNA BINDING PROTEIN S1FA  +2   150  9.5e-10   1
gi|1173350|sp|P42553|S1FA_ORYSADNA BINDING PROTEIN S1FA  +2   149  1.2e-09   1
gi|131035|sp|P08433|PRT1_SCYCAPROTAMINE Z1 (SCYLLIORH... +1    41  0.86      2
gi|225668|prf||1310226Ascyliorhinine Z1 [Scyliorhinus... +1    41  0.86      2
gi|1899127|gb|AAC57008.1|(U86779) pol protein [Human ... -1    61  0.99      1
gi|1041721|gb|AAC49087.1|(U32307) Ras1p [Saccharomyce... -1    60  0.997     1

Use the and icons to retrieve links to Entrez:

E = Retrieve Entrez links (e.g., Medline abstracts, FASTA-formatted sequence reports).
R = Retrieve links to Related sequences (neighbors).
Use the icon (if present) to retrieve links to the Sequence Retrieval System (SRS).
Use the icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .
Use the icon (if present) to retrieve links to the Protein Data Bank database.


to_Entrezto_Relatedto_Related >gi|1173351|sp|P42552|S1FA_SPIOL  DNA BINDING PROTEIN S1FA
            >gi|629495|pir||S47063 s1Fa protein - spinach
            >gi|1361972|pir||S54730 s1Fa protein - spinach
            >gi|498705|emb|CAA56077.1| (X79543) s1Fa [Spinacia oleracea]
            Length = 70

Frame  3 hits (HSPs):                                   __________________
Frame  2 hits (HSPs):        _________________________________________    
                        __________________________________________________
Database sequence:     |             |             |              |       | 70
                       0            20            40             60

  Plus Strand HSPs:

 Score = 179 (63.0 bits), Expect = 8.0e-13, P = 8.0e-13
 Identities = 33/57 (57%), Positives = 45/57 (78%), Frame = +2

Query:   122 SKGFNPALIVLLLVGGLLLTFLIGNYVLYTYAQKTLPPXKKEASLKEEDEKGETEAG 292
             +KG NP LIVLL++GGLLLTFL+GN++LYTYAQK LPP KK+   K++ ++   + G
Sbjct:     8 AKGLNPGLIVLLVIGGLLLTFLVGNFILYTYAQKNLPPKKKKPISKKKMKRERLKQG 64

 Score = 108 (38.0 bits), Expect = 2.7e-05, P = 2.7e-05
 Identities = 20/24 (83%), Positives = 23/24 (95%), Frame = +3

Query:   240 KKKPVSKKKMKKERLKQGVSAPGE 311
             KKKP+SKKKMK+ERLKQGV+ PGE
Sbjct:    47 KKKPISKKKMKRERLKQGVAPPGE 70


to_Entrezto_Relatedto_Related >gi|4371289|gb|AAD18147.1|  (AC006260) unknown protein [Arabidopsis thaliana]
            Length = 76

Frame  3 hits (HSPs):                _____________________________________
Frame  2 hits (HSPs):           ______________________________________    
                        __________________________________________________
Database sequence:     |            |            |            |           | 76
                       0           20           40           60

  Plus Strand HSPs:

 Score = 168 (59.1 bits), Expect = 1.2e-11, P = 1.2e-11
 Identities = 32/57 (56%), Positives = 44/57 (77%), Frame = +2

Query:   122 SKGFNPALIVLLLVGGLLLTFLIGNYVLYTYAQKTLPPXKKEASLKEEDEKGETEAG 292
             +KG NP LIVLL++GGLL+TFLI NYV+Y YAQK LPP KK+   K++ ++ + + G
Sbjct:    14 AKGLNPGLIVLLVIGGLLVTFLIANYVMYMYAQKNLPPRKKKPLSKKKLKREKLKQG 70

 Score = 105 (37.0 bits), Expect = 5.6e-05, P = 5.6e-05
 Identities = 25/56 (44%), Positives = 36/56 (64%), Frame = +3

Query:   147 LFSCLLVGCC*HSSLETMYSTHMHRRPSLL-XKKKPVSKKKMKKERLKQGVSAPGE 311
             L   L++G    + L   Y  +M+ + +L   KKKP+SKKK+K+E+LKQGV  PGE
Sbjct:    21 LIVLLVIGGLLVTFLIANYVMYMYAQKNLPPRKKKPLSKKKLKREKLKQGVPVPGE 76


to_Entrezto_Relatedto_Related >gi|1173348|sp|P42551|S1FA_ARATH  DNA BINDING PROTEIN S1FA
            Length = 76

Frame  3 hits (HSPs):                                     ________________
Frame  2 hits (HSPs):          _______________________________________    
Annotated Domains:      _________________________________________________ 
                        __________________________________________________
Database sequence:     |            |            |            |           | 76
                       0           20           40           60
__________________

Annotated Domains:
   DOMO                 DM04705:                                 1..75
   PRODOM               PD019013: S1FA(2) Q42337(1) Q9ZQC9(1)    8..48
   PRODOM               PD026675: S1FA(2)                        50..75
__________________


  Plus Strand HSPs:

 Score = 165 (58.1 bits), Expect = 2.4e-11, P = 2.4e-11
 Identities = 34/59 (57%), Positives = 43/59 (72%), Frame = +2

Query:   116 AGSKGFNPALIVLLLVGGLLLTFLIGNYVLYTYAQKTLPPXKKEASLKEEDEKGETEAG 292
             A +KG NP LIVLL+VGG LL FLI NYVLY YAQK LPP KK+   K++ ++ + + G
Sbjct:    12 AEAKGLNPGLIVLLVVGGPLLVFLIANYVLYVYAQKNLPPRKKKPVSKKKLKREKLKQG 70

 Score = 102 (35.9 bits), Expect = 0.00012, P = 0.00012
 Identities = 19/24 (79%), Positives = 22/24 (91%), Frame = +3

Query:   240 KKKPVSKKKMKKERLKQGVSAPGE 311
             KKKPVSKKK+K+E+LKQGV  PGE
Sbjct:    53 KKKPVSKKKLKREKLKQGVPVPGE 76


to_Entrezto_Relatedto_Related >gi|11357802|pir||T45877  hypothetical protein F4P12.70 - Arabidopsis thaliana
            >gi|6729488|emb|CAB67644.1| (AL132966) hypothetical protein
            [Arabidopsis thaliana]
            Length = 76

Frame  3 hits (HSPs):                                     ________________
Frame  2 hits (HSPs):          _______________________________________    
                        __________________________________________________
Database sequence:     |            |            |            |           | 76
                       0           20           40           60

  Plus Strand HSPs:

 Score = 165 (58.1 bits), Expect = 2.4e-11, P = 2.4e-11
 Identities = 34/59 (57%), Positives = 43/59 (72%), Frame = +2

Query:   116 AGSKGFNPALIVLLLVGGLLLTFLIGNYVLYTYAQKTLPPXKKEASLKEEDEKGETEAG 292
             A +KG NP LIVLL+VGG LL FLI NYVLY YAQK LPP KK+   K++ ++ + + G
Sbjct:    12 AEAKGLNPGLIVLLVVGGPLLVFLIANYVLYVYAQKNLPPRKKKPVSKKKLKREKLKQG 70

 Score = 102 (35.9 bits), Expect = 0.00012, P = 0.00012
 Identities = 19/24 (79%), Positives = 22/24 (91%), Frame = +3

Query:   240 KKKPVSKKKMKKERLKQGVSAPGE 311
             KKKPVSKKK+K+E+LKQGV  PGE
Sbjct:    53 KKKPVSKKKLKREKLKQGVPVPGE 76


to_Entrezto_Relatedto_Related >gi|1173349|sp|P42554|S1FA_MAIZE  DNA BINDING PROTEIN S1FA
            Length = 63

Frame  3 hits (HSPs):                      _______________________________
Frame  2 hits (HSPs):   _____________________________________________     
                        __________________________________________________
Database sequence:     |               |              |               |   | 63
                       0              20             40              60

  Plus Strand HSPs:

 Score = 150 (52.8 bits), Expect = 9.5e-10, P = 9.5e-10
 Identities = 28/57 (49%), Positives = 39/57 (68%), Frame = +2

Query:   122 SKGFNPALIVLLLVGGLLLTFLIGNYVLYTYAQKTLPPXKKEASLKEEDEKGETEAG 292
             +KG NP ++V L+V   LL F +GNY LY YAQKTLPP KK+   K++ +K + + G
Sbjct:     1 NKGLNPGMVVXLVVASFLLIFFVGNYALYXYAQKTLPPKKKKPVSKKKLKKEKLKQG 57

 Score = 115 (40.5 bits), Expect = 4.8e-06, P = 4.8e-06
 Identities = 24/38 (63%), Positives = 31/38 (81%), Frame = +3

Query:   201 YSTHMHRRPSLL-XKKKPVSKKKMKKERLKQGVSAPGE 311
             Y+ + + + +L   KKKPVSKKK+KKE+LKQGVSAPGE
Sbjct:    26 YALYXYAQKTLPPKKKKPVSKKKLKKEKLKQGVSAPGE 63


to_Entrezto_Relatedto_Related >gi|1173350|sp|P42553|S1FA_ORYSA  DNA BINDING PROTEIN S1FA
            Length = 76

Frame  3 hits (HSPs):                            _________________________
Frame  2 hits (HSPs):           ______________________________________    
Annotated Domains:      _________________________________________________ 
                        __________________________________________________
Database sequence:     |            |            |            |           | 76
                       0           20           40           60
__________________

Annotated Domains:
   DOMO                 DM04705:                                 1..75
   PRODOM               PD019013: S1FA(2) Q42337(1) Q9ZQC9(1)    15..48
   PRODOM               PD026675: S1FA(2)                        50..75
__________________


  Plus Strand HSPs:

 Score = 149 (52.5 bits), Expect = 1.2e-09, P = 1.2e-09
 Identities = 29/57 (50%), Positives = 40/57 (70%), Frame = +2

Query:   122 SKGFNPALIVLLLVGGLLLTFLIGNYVLYTYAQKTLPPXKKEASLKEEDEKGETEAG 292
             +KG NP  IVLL+V  LL+ F +GNY LY YAQKTLPP KK+   K++ ++ + + G
Sbjct:    14 NKGLNPGTIVLLVVATLLILFFVGNYALYMYAQKTLPPRKKKPVSKKKLKREKLKQG 70

 Score = 118 (41.5 bits), Expect = 2.3e-06, P = 2.3e-06
 Identities = 24/38 (63%), Positives = 32/38 (84%), Frame = +3

Query:   201 YSTHMHRRPSLL-XKKKPVSKKKMKKERLKQGVSAPGE 311
             Y+ +M+ + +L   KKKPVSKKK+K+E+LKQGVSAPGE
Sbjct:    39 YALYMYAQKTLPPRKKKPVSKKKLKREKLKQGVSAPGE 76


to_Entrezto_Relatedto_Related >gi|131035|sp|P08433|PRT1_SCYCA  PROTAMINE Z1 (SCYLLIORHININE Z1)
            >gi|85457|pir||S00016 protamine Z1 - smaller spotted catshark
            >gi|64303|emb|CAA29099.1| (X05611) protamine Z1 (AA 1-51)
            [Scyliorhinus canicula]
            Length = 51

Frame  1 hits (HSPs):         ________________________________________    
Annotated Domains:      ________________________________________________  
                        __________________________________________________
Database sequence:     |                  |                   |           | 51
                       0                 20                  40
__________________

Annotated Domains:
   PRODOM               PD050534: PRT1_SCYCA                     1..49
__________________


  Plus Strand HSPs:

 Score = 41 (14.4 bits), Expect = 2.0, Sum P(2) = 0.86
 Identities = 7/16 (43%), Positives = 12/16 (75%), Frame = +1

Query:    85 RRQSPSFLRSRGFKRV 132
             ++Q+P FLR R  +R+
Sbjct:     8 KKQAPCFLRRRHLRRL 23

 Score = 38 (13.4 bits), Expect = 2.0, Sum P(2) = 0.86
 Identities = 8/25 (32%), Positives = 13/25 (52%), Frame = +1

Query:   205 LHICTEDPPSXKKRSQSQRRR*KRR 279
             L++C  D     +R +  RR  K+R
Sbjct:    23 LNVCKRDTSKTYRRRRHVRRLPKKR 47


to_Entrezto_Related >gi|225668|prf||1310226A  scyliorhinine Z1 [Scyliorhinus canicula]
            Length = 50

Frame  1 hits (HSPs):         ________________________________________    
                        __________________________________________________
Database sequence:     |                   |                   |          | 50
                       0                  20                  40

  Plus Strand HSPs:

 Score = 41 (14.4 bits), Expect = 2.0, Sum P(2) = 0.86
 Identities = 7/16 (43%), Positives = 12/16 (75%), Frame = +1

Query:    85 RRQSPSFLRSRGFKRV 132
             ++Q+P FLR R  +R+
Sbjct:     7 KKQAPCFLRRRHLRRL 22

 Score = 38 (13.4 bits), Expect = 2.0, Sum P(2) = 0.86
 Identities = 8/25 (32%), Positives = 13/25 (52%), Frame = +1

Query:   205 LHICTEDPPSXKKRSQSQRRR*KRR 279
             L++C  D     +R +  RR  K+R
Sbjct:    22 LNVCKRDTSKTYRRRRHVRRLPKKR 46


to_Entrezto_Relatedto_Related >gi|1899127|gb|AAC57008.1|  (U86779) pol protein [Human immunodeficiency virus
            type 1]
            Length = 69

Frame -1 hits (HSPs):             ___________________________             
                        __________________________________________________
Database sequence:     |             |              |             |       | 69
                       0            20             40            60

  Minus Strand HSPs:

 Score = 61 (21.5 bits), Expect = 4.6, P = 0.99
 Identities = 14/36 (38%), Positives = 19/36 (52%), Frame = -1

Query:   195 FPMRNVNSNPPTSRRTIKAGLNPFEPARSKEGGTLS 88
             FP     +N PTSR+    G NP   A ++  GTL+
Sbjct:    16 FPTEQARANSPTSRKLQVRGDNPRSEAGAEGQGTLN 51


to_Entrezto_Relatedto_Related >gi|1041721|gb|AAC49087.1|  (U32307) Ras1p [Saccharomyces cerevisiae]
            Length = 65

Frame -1 hits (HSPs):       ______________________________________________
                        __________________________________________________
Database sequence:     |              |               |              |    | 65
                       0             20              40             60

  Minus Strand HSPs:

 Score = 60 (21.1 bits), Expect = 6.0, P = 1.0
 Identities = 14/59 (23%), Positives = 27/59 (45%), Frame = -1

Query:   177 NSNPPTSRRTIKAGLNPFEPARSKEGGTLSANSKSSAMDERRXGTDLRXEESR-CSFLC 4
             N+N   ++ +     N  + +R  +   L++ SK SA  ++    + R E S  C  +C
Sbjct:     7 NNNEGNTKYSSNGNGNRSDISRGNQNNALNSRSKQSAEPQKNSSANARKESSGGCCIIC 65


Parameters:
  filter=none
  matrix=BLOSUM62
  V=50
  B=50
  E=10
  gi
  H=1
  sort_by_pvalue
  echofilter

  ctxfactor=5.95

  Query                        -----  As Used  -----    -----  Computed  ----
  Frame  MatID Matrix name     Lambda    K       H      Lambda    K       H
   Std.    0   BLOSUM62                                 0.318   0.135   0.401  
   +3      0   BLOSUM62        0.318   0.135   0.401    0.336   0.146   0.467  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +2      0   BLOSUM62        0.318   0.135   0.401    0.331   0.146   0.470  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +1      0   BLOSUM62        0.318   0.135   0.401    0.360   0.157   0.582  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -1      0   BLOSUM62        0.318   0.135   0.401    0.338   0.143   0.453  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -2      0   BLOSUM62        0.318   0.135   0.401    0.347   0.152   0.514  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -3      0   BLOSUM62        0.318   0.135   0.401    0.351   0.159   0.554  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a

  Query
  Frame  MatID  Length  Eff.Length     E    S W   T  X   E2     S2
   +3      0      154       141       10.  73 3  12 22  0.12    33
                                                    30  0.11    36
   +2      0      154       143       10.  73 3  12 22  0.12    33
                                                    30  0.11    36
   +1      0      155       144       10.  73 3  12 22  0.12    33
                                                    30  0.11    36
   -1      0      155       143       10.  73 3  12 22  0.12    33
                                                    30  0.11    36
   -2      0      154       143       10.  73 3  12 22  0.12    33
                                                    30  0.11    36
   -3      0      154       143       10.  73 3  12 22  0.12    33
                                                    30  0.11    36


Statistics:

  Database:  /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
    Title:  nr
    Release date:  unknown
    Posted date:  4:06 PM CST Feb 28, 2001
    Format:  BLAST
  # of letters in database:  197,782,623
  # of sequences in database:  625,274
  # of database sequences satisfying E:  10
  No. of states in DFA:  589 (58 KB)
  Total size of DFA:  165 KB (192 KB)
  Time to generate neighborhood:  0.01u 0.01s 0.02t  Elapsed: 00:00:00
  No. of threads or processors used:  6
  Search cpu time:  138.00u 1.33s 139.33t  Elapsed: 00:00:39
  Total cpu time:  138.02u 1.36s 139.38t  Elapsed: 00:00:39
  Start:  Wed Jan 16 18:26:25 2002   End:  Wed Jan 16 18:27:04 2002

Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000