Onanga R, Poaty-Mavongou V, Souquiere S, Kornfeld C, Muller-Trutwin M, Rouquet P, Apetrei C, Simon F, Mboup SJ; International Conference on AIDS.
Int Conf AIDS. 2000 Jul 9-14; 13: abstract no. TuPeA3029.
R. Onanga, Laboratoire De Retrouirologie Centre, International De Recherches Medicales, PO Box 769, Franceville, Gabon, Tel.: +241 677 092, Fax: +241 677 292, E-mail: onanga@cirmf.sci.ga
Background: HIV dynamics during primary infection have a predictive value for the outcome of the disease. However the study of primary infection is difficult, only a few patients being diagnosed as HIV-infected at this early stage of infection. Animal models for the study of viral and immune system dynamics during the primary are badly needed and were already developed during the last years. Here we present an alternative model of SIVmndGB1 primary infection in M. sphinx. SIVmndGB1 was characterised 12 years ago from a wild M. sphinx in CIRMF Gabon. This virus is the representant of an independent lineage of SIV. Looseness=-100000 Methods: GB1 virus was obtained from its original host (GB1) still living and symptom free. We developed an original approach for viral stock preparation to be used to infect the target animals. GB1 infectious plasma was titered on sup T1 cell line at 4 TCID50. In order to obtain a large amount of highly infectious viral stock, we inoculated 10 ml of GB1 blood to a new M. sphinx. Plasma of this mandrills was collected (25 ml) at day 11 of primary infection allowing us to obtain aliquots of 3,000 TCID 50/ml. Subsequently, four monkeys received 1 ml of this viral stock and were bleeded at days 0, 4, 7, 10, 14, 17, 21, 28, 32, 60 and 180. Investigations consisted of viral isolation, quantitation of p24 Ag, RT-PCR, peptide ELISA for the detection of antibodies directed to gp36 and V3 loop and CD4/CD8 counts on peripheral blood and lymph nodes. Results: All included animals developed a primary infection with GB1. The pick of viremia was observed at days 7-10 by HIV-1 p24 Ag, RT activity in plasma and RNA detection by PT-PCR. P24 Ag positivity and plasma RNA were detected until D60. Anti-gp36 antibodies were detected at days 28-32 and the anti-V3 seroconversion starting from D32. We did not observed any modification in CD4, CD8 or CD4/CD8 ratio neither in blood nor lymph nodes. Conclusion: We developed virological and immunological tools to study the SIVmndGB1 infection in Mandrillus sphinx. The virus replicate at high titer during the primary infection. However, after this stage the viral dynamics is well controlled. This new animal model of primary SIV infection helps in understanding the early phase of lentiviral infections and offers a comparative non-pathogenic model.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Animals
- Gabon
- HIV Infections
- HIV Seropositivity
- Humans
- Mandrillus
- Models, Biological
- Simian Acquired Immunodeficiency Syndrome
- Simian immunodeficiency virus
- Viremia
Other ID:
UI: 102238875
From Meeting Abstracts