Ngansop C, Ndembi N, Ichimura H, Kenmogne P, Kaptue L; IAS Conference on HIV Pathogenesis and Treatment (2nd : 2003 : Paris, France).

Antivir Ther. 2003; 8 (Suppl.1): abstract no. 291.

Faculty of Medicine and Biomedical sciences, Yaounde, Cameroon

Mandrillus sphinx, a large primate living in Cameroon and Gabon, and belonging to papionini tribe, was reported to be infected with a simian immunodeficiency virus (SIVmndGB1). Recently, we have reported a novel highly divergent SIVmnd virtually different to SIVmnd-1 and designated SIVmnd-2. To determine the prevalence of SIVmnd-2, we carried out a survey on domesticated M. sphinx in southern Cameroon. From March to August 2001, blood was drawn from 28 nonhuman primates: 13 M. sphinx, five Cercopithecus nicti-tans and 10 Pan troglodytes troglodytes were kept as household pet in southern Cameroon. DNA of sero-reactive samples was isolated from whole blood or PBMCs. The PCR was carried out on pol-inte-grase region (approximately 288 bp). Amplified DNA was cloned and purified, then sequenced on both strands using ABI 310 sequencer. Newly derived SIVmnd-2 nucleotides were aligned with reference sequences from Los Alamos HIV/SIV using Clustal W with minor adjustment for protein sequences. A phylogenetic tree was constructed by the neighbour-joining method. Three seropositive M. sphinx 3/13 (23.1%) were confirmed by western blotting analysis, with New Lav BLot HIV-1 and HIV-2. Phylogenetical analysis showed that these strains clustered significantly in pol-integrase with SIVmnd-2 reference sequence (bootstrap >70%). In an epidemiolog-ical survey of human immunodefieciency virus (HIV) a case of a human infected with a virus serologically related to SIVmnd has been reported from Cameroon, suggesting that mandrills may represent a viral reservoir for human. To confirm this hypothesis, further sero-epidemiological survey of human samples using SIVmnd peptide would be needed.

Publication Types:

• Meeting Abstracts

Keywords:

• Amino Acid Sequence
• Animals
• Base Sequence
• Cameroon
• Cercopithecus
• Gabon
• Genes, pol
• HIV
• HIV-2
• Humans
• Mandrillus
• Primates
• Simian immunodeficiency virus
• genetics

Other ID:

• GWAIDS0022951

UI: 102262575

From Meeting Abstracts