Fetal liver tissue from C57/B6 mice of E11.5 (n=12), E12.5 (n=9), E13.5 (n=9), E14.5 (n=6), E15.5 (n=6), E16.5 (n=3), E17.5 (n=3), E18.5 (n=3), the birth day (n=3), 3 days (n=3), 7 days (n=3), 14 days (n=3) and 21 days (n=3) after birth, as well as liver from mature mice, 18 weeks after birth (n=3), were prepared under the binocular microscope and pooled.
Growth protocol
Mouse embryos at various stages of gestation were obtained by mating random-bred C57/B6 animals. Noon of the following day of vaginal plug appearance was considered to be embryonic day 0.5. The C57/B6 mouse strains were bred and maintained to obtain embryos at National Institute of Biological Sciences (NIBS) at Beijing. All animal experiments in this study were approved by the Institutional Animal Care and Use Committee (IACUC) of NIBS.
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted using TRIZol reagent according to the manufacturer's instruction from Invitrogen.
Label
Biotin
Label protocol
One ug of total RNA was annealed to oligo(dT) and transcribed using SurperScript II reverse transcriptase (Invitrogen, Carlsbad, CA), Labeling, hybridization, washing and signal scan on the microarrays were performed according to the manufacturer's instructions.
Hybridization protocol
The pooled RNA sample were labelled and hybridized to Mouse430 2.0 high-density oligonucleotide arrays (Affymetrix). One ug of total RNA was annealed to oligo(dT) and transcribed using SurperScript II reverse transcriptase (Invitrogen, Carlsbad, CA), Labeling, hybridization, washing and signal scan on the microarrays were performed according to the manufacturer's instructions.
Scan protocol
Primary image analysis of the arrays was performed using GENECHIP 3.2 (Affymetrix, Santa Clara, CA).
Description
gene expression data from fetal liver, embryonic day 11.5
Data processing
The data were analyzed and normalized with RMA software using default settings.
