Wheat microsatellite WMC 25/06/01
wmc(13g3.2)
Plate reference : 13g3.2
WMC locus number : WMC Length of sequence : 166
Last updated : Wed, Aug 27, 1997 at 10:43 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and Rsa1 at 3' end. Multi ple inserts (2) joined 173-190 by linkers - this is the second insert. Matches part of 5E6. No substantial microsatellite.
PCR Comments :
Length of sequence : 166 Consensus sequence is :
..
CGCGCTCTCC TTCGATGACG AGGTCGAAGG CGGCGAGGAC GGCGGCGCGG
AGGGCGTCGA GGTGGGCGGC GAGGATGAGG AGATCGACGT CGACGAGTGG
AGGAGTGTCT TCCCCAATGA CCCCGACAAC GGCACTGGCC CAAACCCGAC
TCGTGGCACA CTGTAC
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wmc(13h3)
Plate reference : 13h3
WMC locus number : WMC Length of sequence : 340
Last updated : Mon, Dec 29, 1997 at 6:06 pm
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3'. Bases 248-338 (complement) (ie 3' side of small microsatellite) match part of 2e9. No substantial microsatellite. Bases1-125 match part of 14E6 (complement) Bases 13 to 45 show homology to 14e6 bases 148 to 180 (complement) (33 in longest run, maxScore = 62).
PCR Comments :
Length of sequence : 340 Consensus sequence is :
..
CACACCATTC CACCTTCCCT TATACTAGGT TTACATCATT AGTGGTCTCT
CTACTCCACA TGCACACACT CCCTCTCACA CGCAAATGTG CGCACAAACA
CACACAAACA CGCACAAACA CCCATCTAGC TATCTGGACC CTTCTTTTAA
GACACACACC TTGCATATTC TCTACAGCTC TAGATATGTA TATACCCCCC
TCTCTAGCTC AGATGCACAC CAAAATTCTC CATCTCTCTA ACTCACACAT
CTCTCCCCAT GCCCGCATGT GTATCTCACA CACTCACTCT CTGATTATGT
CTATGTGTCT CCACGTTACA CAACACAAAG CCCCATGTAC
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wmc294(13a4)
Plate reference : 13a4
WMC locus number : WMC294 Length of sequence : 250
Last updated : Mar 17 Nov 1998 at 10:52
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 1-158 (complement) (ie. 5' side of microsatellite) match part of 7A3(3) - forward primer has 4 mismatches to 7A3(3) including 3' base.. Complex microsatellite (TC)8(CA)7(GCA)2(CA)2(GCA)(CA).
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide.
Forward primer details :
ccgccTgTgTgAAATTTTTgTT at 8 length 22 bases, Tm 65
Reverse primer details :
TTAAAAgcTgTcTcATgcgAAg at 213 length 22 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 227 bases.
Length of sequence : 250 Consensus sequence is :
..
GATTGTGCCG CCTGTGTGAA ATTTTTGTTT GCAAGTTCAG TGTGCTATGA
TGTTCTATAT GATTGTGTCA ACTATATAAG TTTTTACTGA GCATCAGCAA
TGCATATGGC TAAAAGATGT ATTTACGAGC ACCGACCGAT GGGTCTCTCT
CTCTCTCTCA CACACACACA CACGCACGCA CACACGCACA AGTGGGACCC
ATTGAATTAT TTCTTCGCAT GAGACAGCTT TTAATTAGGT TCCACTGTAC
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wmc295(13b4)
Plate reference : 13b4
WMC locus number : WMC295 Length of sequence : 238
Last updated : Mar 17 Nov 1998 at 10:52
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)8 144 to 159, (CA)9 162 to 179.
PCR Comments : 51C anneal, product.
Forward primer details :
ccATAAgTgTTTgcgTTTATTcc at 73 length 23 bases, Tm 61
Reverse primer details :
AATgcAcTATTTTTATAgcTTTgT at 187 length 24 bases, Tm 56
Expected size of amplified fragment (Chinese Spring=) 138 bases.
Length of sequence : 238 Consensus sequence is :
..
GTACGAGTGC ACATGCATGC TATCACACAC GAATTGGAAA GAGTTTCAAA
ATTTGTAGTC AGCTCATGTG CACCATAAGT GTTTGCGTTT ATTCCCCAAA
AAGGAGAATA TTACGTTTAC ACTAAGCAAG AAACAAACAC AAACACACAC
ACACACACAA ACACACACAC ACACACACAA ACACACACAA AGCTATAAAA
ATAGTGCATT TCTATTCAAT TTTGGTCATT AGCGTAGA
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wmc(13c4)
Plate reference : 13c4
WMC locus number : WMC Length of sequence : 242
Last updated : Wed, Aug 27, 1997 at 10:46 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)9 193 to 210. No room for primers.
PCR Comments :
Length of sequence : 242 Consensus sequence is :
..
GTACTAGCGC TATACCAAGG ACCTGAGCTC AAATCCATCA TCCACCACCC
TCTTTTTCAT CACACAAGGC GAGTTGATAT TCATGCCTCA AGGAGCTGGA
TATTGGGAAT TCTCCTGCAG TCTCTTGCGA CTCTACTGTT TGCTCTTTGG
ACAGTGTTTC AGGTATGCAT CTCCCCCTGT GCGCACATTC AGGTGTGTGT
GTGTGTGTGT GCGCGCGCGC GCGTGTGTGT GTGTGTCATG TG
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wmc(13d4)
Plate reference : 13d4
WMC locus number : WMC Length of sequence : 377
Last updated : Tue, Jul 29, 1997 at 10:21 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments :
Length of sequence : 377 Consensus sequence is :
..
GTACGCGCTC CGCGGCATGG GCTGGGCACT CGGGCTATCG GCGCTCACCG
CCGTCGCCGC CGTGACGTTC TACACCTACT ACCTCATGTC CCGCGTGCTC
GACCACTGCG AGGCCGCCGG CCGCCGCCAC ATCCGCTTCC GCGAGCTCGC
CGCCGACGTC CTCGGTATGC CTTAATTGTA GTCAATTAAT GCGGGCGCTG
GTAATCATGG ACCGACAAAT TTGGCAGCGT GGTTTGATGT GCTGTTTTGG
ATCGGTGTGG TGCGGTAGGG TCCGGGTGGG TGTTCTACGC GGTGGTCACC
GTGCAGACCG CCATCAACGC CGGCATCACC ATCGGCAGCA TCCTCATCGC
CGGCAACTGC CTCCAGGTAA ACCACTG
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wmc(13e4)
Plate reference : 13e4
WMC locus number : WMC Length of sequence : 246
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 44-140 have weak homology to 13e4. (CA)12 11 to 34. No room for primers. Bases 172 to 216 show homology to 12d2 bases 69 to 113 (45 in longest run, maxScore = 52). Bases 151-244 show homology to 8d3 (complement). Bases 183 to 193 show homology to 10g3 bases 377 to 387 (11 in longest run, maxScore = 49). Bases 173 to 195 show homology to 26f1 bases 323 to 345 (23 in longest run, maxScore = 52).
PCR Comments :
Length of sequence : 246 Consensus sequence is :
..
CACACATCGA CACACACACA CACACACACA CACACTTAGT TACGAGTCGA
TGGTCGACAT GCAAATGGGG ATCCATGGCA AAACACACAC ACCCTAGTTG
CGAGTCGATG GTCGGCATAC AGCTGGGGAC TCTCGACAAA ACACACATCC
CTAGTTGGGA GTCCATGGTC GTCATGCAAC TGGGGACCCT CGACAAACAC
ACACACCCTC TAGTTGCGAG TCGATGGTCA GCTTGCAACT AGGGAC
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wmc(13f4)
Plate reference : 13f4
WMC locus number : WMC Length of sequence : 237
Last updated : Mar 10 Nov 1998 at 11:12
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimed to insert at 5' end and to Rsa1 at 3' end. Member of 1g1 family. (CA)9 59 to 76. Bases 93 to 150 show homology to 19a4 bases 37 to 94 (58 in longest run, maxScore = 100). Bases 117 to 213 show homology to 21f2 bases 32 to 128 (complement) (97 in longest run, maxScore = 102), true duplicate, 13f4 has priority.
PCR Comments :
Length of sequence : 237 Consensus sequence is :
..
CTCCATCACA CACACATACA CACTAAGACT CCATCTCTCT CTCTCACACA
CACACACCCA CACACACACA CACACACTGA GACTCCATCT CACACACACA
CATGCTCTAG GCGGAGCATT TGTTCCTACC ACCCTTCATC CACCCTTACC
CGTATGATAA ACAATCACCT TAATTTACTT GTATAACATG GACAAATTCC
ACTTTACTTT AGTAGTCAGC AAACTTATCA TCTGTAC
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wmc(13g4)
Plate reference : 13g4
WMC locus number : WMC Length of sequence : 566
Last updated : Tue, Jul 29, 1997 at 10:24 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. No substantial microsatellite. Weak homology to 3b9.
PCR Comments :
Length of sequence : 566 Consensus sequence is :
..
gtaccaagga tctcgcgtct gatgaagaga agatagggaa gGGAAATAAG
AACGGCAACG ACAAGGGTCA CCGGCATAAC CCGGCAAATC AAGGAGGTAA
CAATAAACGT AAGGCTGATG GTAGTCTGGA GTTTGTGGCT AACACCAAAG
CATAGGGCAA CAATCAATGA CGTAAGGGGA GGCCACCTCC TCGGTCAGGC
GGGTCAGGTC CCACCCTCGA ACAGTTGCTA AATGAACCCT GCCCGAGACA
TGGCACCCGG GAGAAGCCGG CCACCCATTT ATGGAAAGAC TGTACGATCA
TGAAGGCGTT CAAGAATTTA AACATGTTCG ACGGTAATCA TGGACCTAGT
AGTGGCTCAG GCGGTGGCGG TTTCCATGGC CCGGGCGGCG GCTCAAGTGG
TGGCGGTTTT CACGGCCCGG GCGGCGGCTC AAGTGGTGGA GGTTTCCATG
GACAGGGCCC TCAAGGCAAT CAAGGCGGCC ATAATCAGCA ATCTGGCCAA
GGTAATCAAC AGCAGCAGCA ATCCGGGTGT CAGAGTAACC CAAAGCAGCT
AAACATTGGG TAGTAC
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wmc(13h4)
Plate reference : 13h4
WMC locus number : WMC Length of sequence : 270
Last updated : Mon, Dec 8, 1997 at 5:28 pm
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 20.10.97.97 Public Domain : Yes
Comments : No sequence 14.7.97. Resequenced. Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)9 155 to 172. Weak match to part of 6a11 outside of microsatellite region. No room for primers.
PCR Comments :
Length of sequence : 270 Consensus sequence is :
..
GTACTCCTAG AAGGACGGCA AGGCATACCA ATAGAGAATG TCGGATCCTC
AAAACAAAGC GGCTGGTCGT GCGTCGGAAA CAATAAGGGG AGGTGTCTAG
ACAAGAGCCC CCCCCCCCCA CACACACACT AAGACTCCAT CTCTCTCTCT
CACTCACACA CACACACACA CAAACACACG CACACGCATG CACACACACC
CACACACATA CACACACTCT AAGACTTCAT CTCTCTCTCT CCCTCTCTCA
CACACACACA CTAAGACTCC
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wmc(13a5)
Plate reference : 13a5
WMC locus number : WMC Length of sequence : 602
Last updated : Tue, Jul 29, 1997 at 10:25 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments :
Length of sequence : 602 Consensus sequence is :
..
gtacacctaa gaatgaaggt ggtagcaaca ctccaagaca tcaccaatcg
tgctcctcct cctcagactc ctctgCAGCA GGTGTAGGTC CCCAAGGGCC
CTCATTAGAA TTAGTCCATG GGCAGTGCAC TCTGACCCAA GACTCCTCAT
CAGTGATCTT GTCCTCTAAA CCATCAGAAA CTGTGAGGCC CACCTACCTC
ATAATGGCCT TGTCACGACA TCGTGCCTTC TTTGCAGCCA CGTGAGCGTT
GTGCACTTTG TCCTTGTGTG CAAGGATGGA GAACCAAACA CTAACACCAT
TCACGAGCTA CCTTTGGTGT TTCAATCTCT TTTACAGGAA TTTCAAGATG
TTTTCCCCGA TGAGCTACCT CCGGGTCTAC CTCCTCTACG AGGCATTGAG
CATCGAATCG ACCTCATCCC CGGAGCGCCA CTTCCAAACA AAGCCCCATA
CCGATCATAA CGCCTCCGCC GCCGGCCGCA GGAGATCGCC AGAGACAGAC
ATGGGGTTAC CAGCGCACGA CGCTTCGACC GAGGACGCCC ACGCCGTCAC
CGCTTCCCCC GCCGCCGGCC CCGCCGAGGA CACTTCAACG GACGCTTCTG
GC
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wmc(13b5)
Plate reference : 13b5
WMC locus number : WMC Length of sequence : 521
Last updated : Mon, Dec 8, 1997 at 5:29 pm
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 20.10.97.97 Public Domain : Yes
Comments : No sequence 14.7.97. Resequenced. Trimmed to insert at both ends. (CA)19 25 to 62, (CA)14 329 to 356, (CA)13 359 to 384. Too difficult to design primers - microsatellites are set inside tandemly repeated (minisatellite-like) element.
PCR Comments :
Length of sequence : 521 Consensus sequence is :
..
ACCTGAAAAA CCCATGAAAA CCAGCACACA CACACACACA CACACACACA
CACACACACA CAGAGCAACC ACGCGGAATC TTCCGTAACC AGACCAAGCC
GGTTCATAGA ATCTTACCAA AACTGCTCGT GGCAGCTACA ATACTGGGCT
GACCTACTAA CCCCACCAAC TGCCACAGGT AACACCGAAG GATCTCTACC
TCCATGTTCG ACGTGTATTT TTTTATTACA ATGTTTATTG AAAAAAGTCG
ACATGTATTG GAAAAAAGAG AAAACAAAAC GAATAAAAAA AACTGGAAGA
TAACTGAAAA AACCCATCAA AATCGAAACA CACACACACA CACACACACA
CACACACGCA CACACACACA CACACACACA CACAAAGCAA CCACGTGGAA
TCTTTCGTAA CCGGACCGAA CTGGTTCATG GAATCTTACC AAAACTACTC
CTAGCAGGCA CAATACTGGG CTGGCCCTTA ACACATGAGT TCATCACATT
TGAAAAATTG ATTATGTTAT G
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wmc(13c5)
Plate reference : 13c5
WMC locus number : WMC Length of sequence : 478
Last updated : Lun 28 DŽc 1998 at 17:11
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end, and to Rsa1 at 3' end. Bases 352-475 (complement) match part of 6B12, bases 54-279 (complement) match 3D9. (GT)8 74 to 89. Too difficult to design primers - microsatellite is set within tandemly repeated (minisatellite) sequence. Bases 249 to 289 show homology to 17h7 bases 417 to 457 (complement) (41 in longest run, maxScore = 82). Bases 229 to 310 show homology to 17g11.1 bases 62 to 143 (complement) (82 in longest run, maxScore = 125). Bases 334 to 369 show homology to 10c9 bases 231 to 266 (36 in longest run, maxScore = 148). Bases 1 to 38 show homology to 21g4 bases 15 to 52 (38 in longest run, maxScore = 52). Bases 118 to 156 show homology to 25a2 bases 99 to 137 (39 in longest run, maxScore = 61). Duplicate with 25a2, 13c5 has priority.
PCR Comments :
Length of sequence : 478 Consensus sequence is :
..
TGTGTGTTTG AAAGAGGAGT AGATAGATTA CTATCAAGAT CGAGGTGCCG
TCCTACTCCT TCGGTGTCGG GTAGTGTGTG TGTGTGTGTG AATGTGTTTG
AGAGAGAAGC CAGTCGGTAG ATTAGTATCA AGATCGAGGT GTCACCCTAC
TCCTTCGGTG TTGGGAAGTG TGTGTGTGTG GTTGTGTGGG TGTGGGTGTG
TGTGTCGGGG TCCGGGGGCA GTGACACTTA CATATCCCTA CTCTTATAAA
AAACAGAGTT GGTGATGATG GTGTTCCTGC CATCCTGCAA TATAGGCCAT
CCGATTTATA CCCGACGGAT AGGAAGGAAA CTGTGGCAAT TTTGCAAAAA
GATACCCACA CCCCTCTCCG CATTTGCAAA TAAGGCATTC CCTCATTCAT
CCTTTTCTCC CACAAGATAA ACTACTCATA CAAATGCATC TTGATGTTCG
GTGCAATGCA TGGCCATCTT GCTAGTAC
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wmc296(13d5.1)
Plate reference : 13d5.1
WMC locus number : WMC296 Length of sequence : 378
Last updated : Mar 17 Nov 1998 at 10:53
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Multiple inserts (2) joined 377- 394 by linkers - this is first insert. (GA)11 233 to 254, (GT)28 177 to 232.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Forward primer details :
gAATcTcATcTTcccTTgccAc at 127 length 22 bases, Tm 64
Reverse primer details :
ATggAggggTATAAAgAcAgcg at 260 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 155 bases.
Length of sequence : 378 Consensus sequence is :
..
CTGCAGTTTC CTCTTTCTTT TTCACAGCTT GCTAGTCTGA GCTAAACTAC
AACCTCTGAG GCTTTTACGC GGCTTTCCGG ATACACGAAA CGTGGTCCAT
GACCATGTCT GCTCCGGTCA CCCGATGAAT CTCATCTTCC CTTGCCACTC
TGCAAAACCA TCCTCCATCC TGGCTTGTGT GTGTGTGTGT GTGTGTGTGT
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGAGAGAGA GAGAGAGAGA
GAGATAGCCC GCTGTCTTTA TACCCCTCCA TCTCCTTTCT CCCACCCCGG
CCTGGTCTTT GTTCAAGTTC AGCTCTGTGT CGCACTCTCC ACTCATCTCC
ACCTCCTCCT CTAGCGATGA CGCCGTAC
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wmc(13d5.2)
Plate reference : 13d5.2
WMC locus number : WMC Length of sequence : 683
Last updated : Mar 17 Nov 1998 at 10:53
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Multiple inserts (2) joined 377- 394 by linkers - this is second insert. No substantial microsatellite.
PCR Comments :
Length of sequence : 683 Consensus sequence is :
..
GTACAAATAC CATCAAGTAT ATACATATCT TCATCTATGG ATCCATGCAT
CAGGTGAGAT TGCAACCTCG TAAGTGCAGC TCTTAATGTT TCATTACCTG
CCATCGATGA AGCTAAAGAT CTTTGCAGGG CTGAATGCGG CGGCGGCGGC
AGCACCGAGA GCGTCAGCAT GAGGAGGAAA AGTTACACTG TGTTTGGATG
TCTCCATTGG AGCCCAGGAA ATGGATTTGG TAATCATCTG ACATCAATAC
AGCTGTTTGG CTGGACACTG AATTCATATT GGAAATGAAA AACCAATTCA
GGCCTGTTTT GGCTACCAAG TAAATGTCCC TCGCACGTTT CGGAGGGCTA
CCCCTTCGAA TTGAACTGAA ATGGCCAACC TCCCGCAAAA CATACCCGTT
CGAGCCCGCA ACTAGAAAAT AAAAGAAAGG AGGGGATGGT GACCTCGCGA
CGACCTAACA GTCAGCAGGC GGCGGCGGCG GCGACCTAGC GGGTAGGTGG
CGGCGGCGGT AACCACCATG GAGTCCGGCG GCGGCTCAGG TGCCCTCCTC
CCCGGTCCTT CCCCTCTTCC CCAACTCCTC TTCTCTGCCC CACTCAGCCA
CACCCCACCG TGGTGGTCGG CGGCGGTGCA GGACTGGCGC TCCCTTGTGG
CATGCCCTGC CGTCCTCCCC GGCCACCGTG CCC
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wmc(13e5)
Plate reference : 13e5
WMC locus number : WMC Length of sequence : 268
Last updated : Mer 14 juin 2000 at 10:34
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 58-243 match part of 7B4(CT)14 67 to 94, (CT)11 222 to 243. No room for primers. Bases 97 to 141 show homology to TW1c05 bases 99 to 143 (complement) (45 in longest run, maxScore = 49).
PCR Comments :
Length of sequence : 268 Consensus sequence is :
..
GTACACATCC AACACAAAAT TCTTGTTGGT CCCTCTCCCT TTCTCTCCAT
CTCTCTCTCT CTCTCCCTCT CTCTCTCTCT CTCTCTCTCT CTCTCACCGA
CAATCTTCAA TTCTGTCGCA CGCATCCAAC ACAAAATTCT TGTTGGTCCC
TCTCGCTTTC TCTCCATCTC TCTCTCTCTT TCTCTCCATC TCTCTCTCTC
TCCCTCTCTC TTTCTCTCCA TCTCTCTCTC TCTCTCTCTC TCTACGACAA
TCTTCAATTC TGTCGCAC
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wmc(13f5)
Plate reference : 13f5
WMC locus number : WMC Length of sequence : 263
Last updated : Mon, Dec 8, 1997 at 5:32 pm
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 20.10.97.97 Public Domain : Yes
Comments : No sequence 14.7.97. Resequenced. Trimmed to insert at both ends. (CA)12 72 to 95, (CT)9 54 to 71. Bases 86- 263 (ie 3' side of microsatellite) match part of 6H4 (member of 1g1 family). No room for primers.
PCR Comments :
Length of sequence : 263 Consensus sequence is :
..
CAAACTAAGA TCCATCTCTC TCTCTCTCAC ACACATACAA ACTAAGAATC
CATCTCTCTC TCTCTCTCTC TCACACACAC ACACACACAC ACACACCACA
CACACACACT AAGACTCCAC CTCACACACA TGCTCTAGGC GGAGCATTTG
TTCCTACTAC TCTTTATCCA ACCTTACCCG TATGATAAAC AATCACCTTT
ATTTACTTGC ATAACATGGA CGGATTCCAC TTTACTTTAG TATTCAGCAA
ACTTATCATC TGT
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wmc297(13g5)
Plate reference : 13g5
WMC locus number : WMC297 Length of sequence : 284
Last updated : Mar 17 Nov 1998 at 10:53
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)29 188 to 245. Bases 117 to 245 show homology to 19h3 bases 14 to 142 (complement) (129 in longest run, maxScore = 57).
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Forward primer details :
TcAAAAgccTcAAAgAAAcTcA at 59 length 22 bases, Tm 61
Reverse primer details :
GATAATAAgAATcccATggAAAcA at 257 length 24 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 220 bases.
Length of sequence : 284 Consensus sequence is :
..
GAGCTGCTAC AAATTGGTGT GTGCGACGAG CTGAAGGACA TCTCCGGGCT
ACCAGCCCTC AAAAGCCTCA AAGAAACTCA GTTTCCTAAT GATGGTGACA
GTTATTACAG TAAAATATTA GAGGTGCAGA GGGAACAGGT GGAGAGGCAG
GTAGCGGAGC ACGTGAGAGT GAACTATTAA ACTCTTTGTG TGTGTGTGTG
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTATGTG
TGTGTGTGTT TCCATGGGAT TCTTATTATC GTAC
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wmc(13h5)
Plate reference : 13h5
WMC locus number : WMC Length of sequence : 325
Last updated : Tue, Jul 29, 1997 at 10:32 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Reverse sequence only. Trimmed to Rsa1 at 5' end, 3' end not located. No substantial microsatellite.
PCR Comments :
Length of sequence : 325 Consensus sequence is :
..
GTACGTTCTG CATGTGTGTA TCTTTGTTGT GAGCGAGAGA ATAGTTGTGT
GGTAGTAGTT TCTAGATAGA GGTTTTACAT TGCGATACCA TGGAACGGAT
ACATGAAAGA ACAAAGCCTA CATGTGGTGG TCTAGTAGTC TACTTTTTCC
TCGAGTAATA TGTAGAGCTG CGGCATAGAG CGGTGGTCAG TTCATCCTTT
TTTTTCTGGG GGGTTGTTTT CCAGGAGCAA TGCTAGAACT AACGTCAGTT
TTTATGTGAA TGAAATCAGT TTGTTTAGGA TTAGCGGGTG AGGCCCACCC
CGGTTAAATT TGTGTGTGTG TGTGT
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wmc(13a6)
Plate reference : 13a6
WMC locus number : WMC Length of sequence : 286
Last updated : Wed, Aug 27, 1997 at 9:42 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. Duplicate of 13D6. No substantial microsatellite.
PCR Comments :
Length of sequence : 286 Consensus sequence is :
..
GTACATCAGC CACCCACGAC GTGCTGCTGC GATGGAACTA GCTAGGCGCC
CGCCTATATA AGCATGAGGT CCGGTGCATG TATTCCAGAC AAGGATACAA
CCTGAGCTAA TCAAGATGGC TTCCTCCACG CCTTACCGTG TCCTACTCTT
GTGCCTCACC ACTTTCCTGC AGGCATGGCT TGCTGCGGCA AAATACCCGC
CGCATAACCC ACCGCCGCCG CCCCCGCCGT TTGAGCTGCC GGAGTCCGAC
GTGAGGGGGA GGTTCTCCAG GTGGGTGATC AAGTAC
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wmc(13b6)
Plate reference : 13b6
WMC locus number : WMC Length of sequence : 421
Last updated : Lun 5 juil 1999 at 17:23
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Member of 1g1 family. (GA)8 229 to 244, (GT)8 339 to 354. Bases 87 to 126 show homology to 17e10 bases 97 to 136 (complement) (40 in longest run, maxScore = 76). Bases 47 to 125 show homology to 12d12 bases 404 to 482 (complement) (79 in longest run, maxScore = 89). Bases 193 to 229 show homology to 26b10.2 bases 88 to 124 (37 in longest run, maxScore = 46).
PCR Comments :
Length of sequence : 421 Consensus sequence is :
..
GTACAGATGA TAAGTTTGCT GACTACTAAA GTAAAGTGGA ATTTGTCCAT
GTTATGCAAG TAAATAAAGG TGATTGTTTA TCATACGGGT AAGGTTGGAT
GAAGGGTGGT AGGAACAAAT GCTCCGCCTA GAGCATGTGT GTGTGAGATG
GAGTCTTAGT GTGTGTGGGG TGTGTGTGTG AGAGAGAGAG AGGAGAGAGA
GATGGAGTCT TAGTTTGTGT GTGTGTGTGA GAGAGAGAGA GAGATGGAGT
CTTAGTGTGT GTGGGGGTGT GCGTGCGTGT GTGCGTGTGT GTGTGTGAGA
GAGAGATGGA GTCTTAGTGT GTGGGGGTGC CTGCGTGCGT GTGTGTGTGT
GTGTGCGCGT GCGTGCGTGC GTGTGTGTGT CGCGGGGTCC TTTGTGGCGG
ATGTAATTTA AGTGTGCATG G
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wmc(13c6)
Plate reference : 13c6
WMC locus number : WMC Length of sequence : 329
Last updated : Wed, Aug 27, 1997 at 10:57 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end, 5' end not located. (CA)38 19 to 94, (CA)11 97 to 118. No room for primers.
PCR Comments :
Length of sequence : 329 Consensus sequence is :
..
TCTCTAGGCC GTGGACtACA CACACACACA CACACACACA CACACACACA
CACACACACA CACACACACA CACACACACA CACACACACA CACACGCACA
CACACACACA CACACACACT TCTATGTTGA TGGGCCTTTT ATGGGCTTTC
ATAGAGACCG CGAAAAATAA TTGGGTCAAA ATAAATCAAC ATTGTGGGAA
TTGAACACAG GACCTCCTGT CTAGATCTTC GATGTAACTA ATTAAGGGAA
TGAGCAAACT AAAGGAAGGA TTGCGTCCTT TTCTTGGTTT GGTTTAGTCC
TTTTCGTTTG GTCAATCTGG GCCAGGTAC
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wmc(13d6)
Plate reference : 13d6
WMC locus number : WMC Length of sequence : 283
Last updated : Tue, Jul 29, 1997 at 10:36 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Duplicate of 13A6. No substantial microsatellite.
PCR Comments :
Length of sequence : 283 Consensus sequence is :
..
CATCAGCCAC CCACGACGTG CTGCTGCGAT GGAACTAGCT AGGCGCCCGC
CTATATAAGC ATGAGGTCCG GTGCATGTAT TCCAGACAAG GATACAACCT
GAGCTAATCA AGATGGCTTC CTCCACGCCT TACCGTGTCC TACTCTTGTG
CCTCACCACT CTCCTGCGGG CATGGCTTGC TGCGGCAAAA TACCCGCCGC
ATAACCCACC GCCGCCGCCC CCGCCGTTTG AGCTGCCGGA GTCCGACGTG
AGGGAGAGGT TCTCCAAGTG GGTGATCAAG TAC
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wmc(13e6)
Plate reference : 13e6
WMC locus number : WMC Length of sequence : 381
Last updated : Tue, Jul 29, 1997 at 10:37 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :
Length of sequence : 381 Consensus sequence is :
..
GCAACCGTGC ATGCTAGACC CTCCGGAGCA AACCATTTGC GACTTCTCTG
GCGGCGCATG TTGCAACAAC ACCGACCTCC CAGTATTTAA TAAAAAACCA
CCACGTTTGA CGGAAACATG ACGAACGTTT GTCGGGACGT CGGCTGTCGA
TCGGGTGCGG GTCAAGATCT GATCCGTTCC CCTCTCGCTG CACTCTCTCC
GTCCTCTCCC TCTCTCTGAA CTAGGCGGCG GCGGCGGCGG TTCCGGAGGG
GGGCGTCGGC GATATGGGTG GTGGGCACAA GTTTGGACGC TGCGGGCTCA
GTCGACAATT GGTTGGGGGG CACCAGTTTC TCGACGCAGG CCGGCTCGCA
TCAGCAGGAG GCACAGCTCG CCCAGTCATC G
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wmc298(13f6)
Plate reference : 13f6
WMC locus number : WMC298 Length of sequence : 475
Last updated : Mar 17 Nov 1998 at 10:53
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GA)16 271 to 302, (GT)18 235 to 270.
PCR Comments : tested 61C anneal, no product; tested 51C anneal, no product. Primer dimer - redesign primers.
Forward primer details :
cTcTTgcATAcAAATgccAggA at 177 length 22 bases, Tm 64
Reverse primer details :
ctAAAcAcTTcTcTgccTAggAcA at 330 length 24 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 177 bases.
Length of sequence : 475 Consensus sequence is :
..
CTGCCTCTGA GGCCAAATCT CTGGGAGCAG GATCGTGCTC ACACGGAATT
CAATGCTAGT GCAACTAATG CATGCATGCA CGCATGTCAG CATGTTGATC
CAGTTAATAA TGCCGTGATG ATATCCATCG TCGATCCGTT GGGCCTTGTT
TCATTGTCCT TATTTCTTCC CTTTGTCTCT TGCATACAAA TGCCAGGAGA
GATTGGGCCG ATCTGAATCT GAGAGATACT ATTTGTGTGT GTGTGTGTGT
GTGTGTGTGT GTGTGTGTGT GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA
GAGGAATAGC TAGAACAGAA TTTGGGTTCT GTCCTAGGCA GAGAAGTGTT
TAGTGATGTC ATTTTGCAAA GGTTTTTGGT TTCTGAAAAG ACCACTACTT
CTCAAGGAAA AGAATCTAGG CACGTTTTAT TTTAAAAATG ATCCCTTCCT
AGACTTTTAG CTTTAGACAA AAAAC
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wmc(13g6)
Plate reference : 13g6
WMC locus number : WMC Length of sequence : 318
Last updated : Wed, Aug 27, 1997 at 11:00 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)23 1 to 46. No room for primers.
PCR Comments :
Length of sequence : 318 Consensus sequence is :
..
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGCAG
GTTTTCCTAC ATGTGAAGCC AACCATCAGG ATGGCCTTGG AGTTTTCTCG
TATAGTAGTT GCTTGGTGCT GGATCTGCAG TATTTTCGGG AATGCTGTTG
GTTCTGCTTG GAGTTTCCTA CATGTGACTT CTTTCATTCT ACTATGAATC
TGCAGTAGTT CTTTCCTCAA CAAATGTAGA TTGTCGTGTA TTGAATGTGA
AAAGAGTTGA AATTGGAACT TCTATTTATT TCATATTATA GCTGAATTTC
GGATCGGCCC TGGGGTAC
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wmc299(13h6)
Plate reference : 13h6
WMC locus number : WMC299 Length of sequence : 382
Last updated : Lun 30 oct 2000 at 16:59
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. Apart from a few indels this sequence (complement) is a duplicate of 9F3.1 - forward primer has no match to 9f3.1, and reverse primer has 2 mismatches including antepenultimate base. (CT)8 187 to 202. Bases 162 to 203 show homology to TW6d06 bases 433 to 474 (complement) (42 in longest run, maxScore = 114).
PCR Comments : 51C anneal, product.
Forward primer details :
gtacTccATgAAcATgTTgAAAg at 1 length 23 bases, Tm 58
Reverse primer details :
gggAcTAAcAAggTTTgcgT at 239 length 20 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 258 bases.
Length of sequence : 382 Consensus sequence is :
..
GTACTCCATG AACATGTTGA AAGTCAAATT AATGAATTTG TTTGATATAC
GCATATCTCT GTTCATGTGT GGATTGCACA CAACATGTTC TTCAATTTAA
ATATTTGAAT GCAAGTTTAT ATCGAAACAT GGTTTATATG AGTCTTTGAT
GCATAAAATG CGACCTCCCC CTCTCCCGGA CTCCTGCTCT CTCTCTCTCT
CTCACCGACA ATCTTCAATT CTGTCGCACG CATCCAACAC GCAAACCTTG
TTAGTCCCTC TCCCTCTCTC TCCATCTCTC TCTCTCTCTC CCTCTCCCTC
TCTCTCTTTG TGTGTGTGTG TGTGGGGGGG TCTTTCTAGT TCGCATGCAT
ATATACTCCA GTCTCTCTCG CACACTATGT AC
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wmc300(8A1)
Plate reference : 8A1
WMC locus number : WMC300 Length of sequence : 239
Last updated : Mon, Mar 16, 1998 at 20:28
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : Contains (GT)19(GA)13 repeat. Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 189-239(ie. 3' side of microsatellite) match part of 1f11 (1 mismatch in reverse primer). (GA)13 180 to 205, (GT)19 142 to 179.
PCR Comments : tested 61C anneal, no product; 51C anneal, product; complex.
Forward primer details :
cAcgAAgcgAcgTAgggTTATT at 22 length 22 bases, Tm 64
Reverse primer details :
cAgAgcAccTTTTgTgTTgAgA at 208 length 22 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 208 bases.
Length of sequence : 239 Consensus sequence is :
..
GTACCGCGGA AGAGTGCAGC TCACGAAGCG ACGTAGGGTT ATTCCTGGCA
GCTTCTTTAC CAGGGACCTC TAGAGGCCCT ACTGGTAAGG GCTTGCCAGA
GTCAAGTGCC GGAAAAATGA AGCGAGAAAC AATGTGCGCG CGTGTGTGTG
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG AGAGAGAGAG AGAGAGAGAG
AGAGAAATCT CAACACAAAA GGTGCTCTGT TGGAATCCC
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wmc(8b1)
Plate reference : 8b1
WMC locus number : WMC Length of sequence : 6
Last updated : Mon, Oct 13, 1997 at 2:00 pm
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : No sequence 15.9.97.
PCR Comments :
No sequence available for this clone
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wmc301(8c1.1)
Plate reference : 8c1.1
WMC locus number : WMC301 Length of sequence : 346
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : contains (AC)14imp. Trimmed to Rsa1 at 5' end. Double insert - this is first insert. Bases 1-119 (complement) (ie. 5' side of microsatellite) match part of 9d1.(CA)8 73 to 88, several small CA runs. Bases 285 to 318 show homology to 26b1.1 bases 110 to 143 (complement) (34 in longest run, maxScore = 32).
PCR Comments : 61C anneal, polymorphic on acrylamide; though complex.
Forward primer details :
TgTAggAgTAggTcgATcTgTc at 22 length 22 bases, Tm 58
Reverse primer details :
AgTccAAgAATccTccTgTcAc at 186 length 22 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 186 bases.
Length of sequence : 346 Consensus sequence is :
..
GTACAGTACA CAACATCTCT CTGTAGGAGT AGGTCGATCT GTCGCTCTCT
CTAACACATA CATGATGTTA AACACACACA CACACACACG CACACACACA
CGCACGCACA CACGCACACA TCTTGGTCCC GCTTTACCTT CTAACTTGAG
GTCTCGGGTT CGATAACTAC ACTTGAGCAT AATTTGTGAC AGGAGGATTC
TTGGACTGGT CAAAATGTTT TCTAGGGTTT GCACGCTTCA CACTCTCTCT
CCAGTATATA TATACACGCT GGAGCCTCAG CGCATGTAGT TGCTCTCTTC
ACACTCTCTC ACCCTCTCCC TCCGTTCCGA ATTACTTGTC GCAGCT
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wmc(8c1.2)
Plate reference : 8c1.2
WMC locus number : WMC Length of sequence : 268
Last updated : Lun 5 juil 1999 at 17:23
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : contains (AC)14imp. Trimmed to Rsa1 at 5' end. Double insert - this is second insert in this clone. Bases 133-226 (ie. 5' side of small microsatellite) match part of 7g5. Bases 131 to 151 show homology to 14f5.2 bases 86 to 106 (21 in longest run, maxScore = 61). No substantial microsatellite. Bases 113 to 137 show homology to 21b6 bases 43 to 67 (25 in longest run, maxScore = 52). Bases 130 to 151 show homology to 26c9 bases 23 to 44 (complement) (22 in longest run, maxScore = 76).
PCR Comments :
Length of sequence : 268 Consensus sequence is :
..
GTACTCCAAC GGTTAACCGG GCATCTGCAA AGTCCATTCA TGTGTTGCAG
CAAAACCCCC TCAAAAACCA GCCCAAAACC CACCAAACTC TCTTCCATGC
TGTAGGTCGT TCGATCACGA TCGTGTGGGC GAAAACCGCA CCTCATTTGG
AGCCTCCTAG CTCCCTCTAC CTATTTATAC GTGACCCTCC CGAAAAAAGC
ACGCAGATCA AACCCTAGAC CCGCTCCCTC TCCGCCGCCG GACACGTCCG
GGCGGAGCCG GACGCGCC
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wmc(8d1)
Plate reference : 8d1
WMC locus number : WMC Length of sequence : 356
Last updated : Lun 2 Nov 1998 at 9:57
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : Contains (CA)51imp. Trimmed to Rsa1 at both ends. (CA)8 45 to 60, (CA)9 77 to 94. Too difficult to design primers Bases 49 to 89 show homology to 10f5.1 bases 306 to 346 (41 in longest run, maxScore = 23).
PCR Comments :
Length of sequence : 356 Consensus sequence is :
..
GTACTTCTTG CTAAAATTAG TTTTTGTACG CGCCCGGCCC GCTCCACACA
CACACACACA CGCATACACA CACATACACA CACACACACA CACACTACAT
ACACACACAC ATACACACAG ACACACTACA CACACACATA CACACAGACA
CACACAAACA CACACACACA CTACACGTCT CACCTCCTAA ATGATTCTAC
AGTGCCTTCT GTTTATCTTA GTAATATGCT AATATAGGGG TTAGCTCGGT
CTACTTTAGC TTAAGAGATG GTGGGTTTTG GTGGCGATGC AGCAATTACT
TCGACATCCG ATCAGCAGTT AAAGTAATTT CCAACAGTCG AACGGATATT
TTGTAC
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wmc302(8e1)
Plate reference : 8e1
WMC locus number : WMC302 Length of sequence : 308
Last updated : Tue, Mar 17, 1998 at 9:48 am
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : Contains (AC)18 and (AC)11 imp. Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)17 148 to 181. Bases 170-268 (complement) ie 3' side of microsatellite match part of 8C12. Reverse primer is outside this region. 8F2 is a duplicate of this.
PCR Comments : tested 61C anneal, no product; 51C anneal, many products, some polymorphic.
Forward primer details :
cAAcccTgTgTAATTgTAAccg at 76 length 22 bases, Tm 61
Reverse primer details :
ggTAggcAcAgAggcAcAAgTA at 279 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 225 bases.
Length of sequence : 308 Consensus sequence is :
..
GAAAGTGAAA AGGTGAACAC TAGAAGTCAG TCGACCGTTC CAAATTTTGG
CCGATCACCG CACGGCCGTC CGATGCAACC CTGTGTAATT GTAACCGTTG
GATCGCAATC CTACATGTCT TCACTTTCCC ATAGTTTTCT CTCGAAACAC
ACACACACAC ACACACACAC ACACACACAC ACTACCCGAC ACCAAAGGAG
TAGGGTGGCA CCTCGATCTT GATAGTAATT TATCTACTGG CTTCTCTCTC
AAACACACAC ACATACACAC ACACTCACTA CTTGTGCCTC TGTGCCTACC
GCGCGTAC
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wmc(8f1)
Plate reference : 8f1
WMC locus number : WMC Length of sequence : 6
Last updated : Mon, Oct 13, 1997 at 2:01 pm
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : No sequence 15.9.97.
PCR Comments :
No sequence available for this clone
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wmc(8g1)
Plate reference : 8g1
WMC locus number : WMC Length of sequence : 491
Last updated : Mon, Mar 16, 1998 at 11:59 am
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : Contains (CT)16. Trimmed to insert at 5' end and to Rsa1 at 3' end. (CT)16 328 to 359. Duplicate of 5H4.
PCR Comments :
Length of sequence : 491 Consensus sequence is :
..
TACCTTGTCA CTCGATCTCC CACCCATGTA GTCCCATAAC GATATCCAGG
GGATCTCTCT ATGACAAACA TACACTCTCT CCTCCCCATG TCTGCATTTT
CTCCGTACGT CTCTCTCGAC CTCTCTCCCT TGTTTGTCAG ACCCCTCTTG
GCCACGTGCT AGGGGTCTTC CTCTCTCGGT TCCAAACAAC CACACACTAT
CTCCTCACCT TGCCTCCATT GTCGAAGATG CATGTGTGAT ATGTTTGCAT
AAGACACGCA CGATTTTTCT CCACTTTTAT CGTGTGTTGT CCATGTCTCT
TTCACACACG CACACACACT TTTTTCACTC TCTCTCTCTC TCTCTCTCTC
TCTCTCTCTC GTCAGGGACT CTGTCACGTC CATAAGCATA TGGATGTTCT
GAAAAGTCAA ACCTCAAAAA AGTTTGACTA GGTATATGTG GAGAAAAACA
TTTACATCTG GAATGATCAT TAGATTCATC ACAACATGTA C
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wmc(8h1)
Plate reference : 8h1
WMC locus number : WMC Length of sequence : 364
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 200-364 (complement) show (weak) homology to part of 2d9. Bases 212 to 227 show homology to 12f11 bases 173 to 188 (16 in longest run, maxScore = 36). (GT)10 57 to 76 plus two small GT runs. First part of sequence containing microsatellite is embedded in minisatelite repeat region. Too difficult to design primers. Bases 161 to 178 show homology to 20a8 bases 88 to 105 (18 in longest run, maxScore = 47). Bases 1 to 22 show homology to 20g5 bases 275 to 296 (complement) (22 in longest run, maxScore = 52).
PCR Comments :
Length of sequence : 364 Consensus sequence is :
..
GTGTGTGTTT GTCGAGGGTC CCAAGTTGCA ACCCGACCAT TGACTCGTAA
CTAGGGGTGT GTGTGTGTGT GTGTGTCGAG GGTCCCCAGT TGCATGTCAA
CCGTCGGCTC ACAACTAGGG GTGTGTGTGT GTTTGTTTGT CAAGGGTCCC
CATTTGCAAT CCGACCATCA ACTCGCAACT AGGGGTGTGT GCATTTGTCA
AGGGTCCCCA TTTGCATGCC GACCATCAAC TCGCAACTAG GGGTGTGTGT
TTGTCGTGGC CCCTAGTTGC AACCCGATCA TCGACTCACA ACTAGGGGTG
TGTGTGTGTG TTTGTTGAGG GTCCCTAGTT GGATGCTGAC TATCGACTCG
CAACTGGGTG TGTG
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wmc(8a2)
Plate reference : 8a2
WMC locus number : WMC Length of sequence : 261
Last updated : Lun 2 Nov 1998 at 10:03
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : Contains a series of (CCN) repeats . Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite. Bases 93 to 100 show homology to 24e1 bases 222 to 229 (complement) (8 in longest run, maxScore = 21).
PCR Comments :
Length of sequence : 261 Consensus sequence is :
..
CGCCGCCGAT GCCGCCACCG CCACCTTTGC CGATCCCGAA ACCGAACCCA
CCTCCCTTGC CAAAGCCTTC ACCAAAACCG CCGCCACCAC CACCACCACC
TCCTCCGCCG CCTCCGCCGC CGCCTAGTCC ACCACCTTTG CCAAAGCCAC
CGCCGAGACC ACCGCCATTT CCGATGCCAC CACCGAGCCC ATGGCCGATG
CCGCCTCCGA GACCGCCACC ATTTCCGAGA CCACCACCAT TGCCATACTC
GCCACCGGTA C
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wmc(8b2)
Plate reference : 8b2
WMC locus number : WMC Length of sequence : 143
Last updated : Tue, Mar 17, 1998 at 10:05 am
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : Contains (CT)14 imp and (CT)12. Trimmed to insert at 5' end. (CT)10 1 to 20, (CT)12 92 to 115. Matches (part of) 11f12.
PCR Comments :
Length of sequence : 143 Consensus sequence is :
..
CTCTCTCTCT CTCTCTCTCT GTCTCTCTGA CAATCTTCAG TTCTGTCGCA
CGCATCCAAC ACACAAACCG TGTTGGTCCC TCTCCCTCCA TCTCTCTCTC
TCTCTCTCTC TCTCTGACAA TCTTCAGTTC TGTCGCACGC GCC
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wmc(8c2)
Plate reference : 8c2
WMC locus number : WMC Length of sequence : 142
Last updated : Mon, Mar 16, 1998 at 12:02 pm
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : contains (CA)12. Trimmed to insert at both ends. (CA)12 41 to 64. (Near) duplicate of 9d1.
PCR Comments :
Length of sequence : 142 Consensus sequence is :
..
GTCGATCTGT CGCTCTCTCT AACACACACA TGCTGTTAAA CACACACACA
CACACACACA CACATACACG CACACGCACA CACCTTGGTC CCGTTGCACC
TTCTAACGTG ACTTATTACA CCTAGACGGA GGGAGTAGTA AG
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wmc(8d2)
Plate reference : 8d2
WMC locus number : WMC Length of sequence : 264
Last updated : Mon, Oct 13, 1997 at 2:02 pm
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : Contains (GCG)5 imp. Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :
Length of sequence : 264 Consensus sequence is :
..
GCGTTGAGAG GACCAGGCTG GTGCGGCGAG GACCAAATTG GGAGCGGCGG
AGGCGGCGGC CAGGCTGGGG CGGCGGCGAT CAAATTGGGA GCGGCGGCGA
CCAAACTGGG CAAGGACGAC GGGGAAGAGA CTGGCGAAGG GGCCTGCTGG
TGGATGGACT AGTAGCTGGA GGAGGTGGAG GGCGGAGGAG GTGGCTGGCC
TGGCGGAGGA AGACGACGAC TCTCAGCGAC AACTCTCGTT TTCAAATCAG
GGGTATTTTT GTAC
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wmc(8e2)
Plate reference : 8e2
WMC locus number : WMC Length of sequence : 501
Last updated : Mon, Oct 13, 1997 at 2:02 pm
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : Contains (GCC)4 imp and (GGC)3 and (CAA)3. Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :
Length of sequence : 501 Consensus sequence is :
..
GCACGATATT GCAGCTGCGA GAGATGTCGA TCCCGGACGG CGGCAGCGGC
GTCTTCTCTG GTGGGAGGCC GGCGGCGGCG ATCTCGTGCA TCGGCGGCAG
GAAGATCGTA CGTAGGCGGC GGCTTCTCCT CTGGGAGTTC GAGGGGATTG
AGGGGAGCGG CGTTGTATGT TTTTTTTGGG TGCGTGCCTA GGGACCCTAG
AGGGGGGACG AAAAAAACCA GCGAAATAAA ACGGTTGGAA GTGGTGGGAC
GAAAATAAAC CGTGGATACT ATTCTACCAA CTCAGACATT AGGAGTAGAG
ATATCAATGA TAAGTTTGTT TTATTGTTCG TTCATATGAG CAACAGATCT
ATAAAGGTTT CAGCATTTCT TGATACATGT CTACAGCAAG AATGCAGCGA
CAGCAATTCT CATATGAGTG TGAACTTTCC CTTTTCTCAA CAACAATCCT
TACAAGGTGG TGGTTTTAAT TTTTGTTTGA CAAGTTGAGA TTGATCAGTA
C
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wmc(8f2)
Plate reference : 8f2
WMC locus number : WMC Length of sequence : 308
Last updated : Mon, Mar 16, 1998 at 12:06 pm
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : Contains (AC)18 and (AC)11imp. Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)17 148 to 181. Duplicate of 8e1 (use 8e1 primers).
PCR Comments :
Length of sequence : 308 Consensus sequence is :
..
GAAAGTGAAA AGGTGAACAC TAGAAGTCAG TCGACCGTTC CAAATTTTGG
CCGATCACCG CACGGCCGTC CGATGCAACC CTGTGTAATT GTAACCGTTG
GATCGCAATC CTACATGTCT TCACTTTCCC ATAGTTTTCT CTCGAAACAC
ACACACACAC ACACACACAC ACACACACAC ACTACCCGAC ACCAAAGGAG
TAGGGTGGCA CCTCGATCTT GATAGTAATT TATCTACTGG CTTCTCTCTC
AAACACACAC ACATACACAC ACACTCACTA CTTGTGCCTC TGTGCCTACC
GCGCGTAC
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wmc(8g2)
Plate reference : 8g2
WMC locus number : WMC Length of sequence : 6
Last updated : Mon, Oct 13, 1997 at 2:02 pm
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : No sequence 15.9.97.
PCR Comments :
No sequence available for this clone
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wmc(8h2)
Plate reference : 8h2
WMC locus number : WMC Length of sequence : 6
Last updated : Mon, Oct 13, 1997 at 2:02 pm
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : No sequence 15.9.97.
PCR Comments :
No sequence available for this clone
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wmc(8a3)
Plate reference : 8a3
WMC locus number : WMC Length of sequence : 176
Last updated : Mon, Oct 13, 1997 at 2:02 pm
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :
Length of sequence : 176 Consensus sequence is :
..
TCTGGAGACC ACCCTCCCGT GCCTCCAGAG GCGACATGGC CTTGTACGCT
TTAGCCACAA TAGTCCGAGA GGACGCATGA TGGCCATAGG TTCCCGAGTG
GATATCGTCA AGGATTATCC GACCTTCTTC TGGCGTTATA CATTTCTGAC
CGACTCCAGT CGCGCTTTCT CTGTAC
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wmc(8b3)
Plate reference : 8b3
WMC locus number : WMC Length of sequence : 6
Last updated : Mon, Oct 13, 1997 at 2:02 pm
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : No sequence 15.9.97.
PCR Comments :
No sequence available for this clone
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wmc(8c3)
Plate reference : 8c3
WMC locus number : WMC Length of sequence : 155
Last updated : Mon, Oct 13, 1997 at 2:03 pm
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : Contains (CGC)5. Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :
Length of sequence : 155 Consensus sequence is :
..
GTGACAGCCT CTCATTGCAC GCCGCCGCCG CCGCTTCAAG CACTGCCGGT
TCCCTCATCC CACGATAAAC AACAGCATAG CCTCCTAGAT ATTTTTCCCA
TATAATCACG ACGCACTGCT CCAATAGCTC ATCGAGATAA ATGCATGTGT
GGTAC
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wmc(8d3)
Plate reference : 8d3
WMC locus number : WMC Length of sequence : 309
Last updated : Lun 2 Nov 1998 at 10:06
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : Contains (GT)12. Trimmed to insert at 5' end and to Rsa1 at 3' end.(GT)12 115 to 138. Bases 14-114 (complement) homologous to part of 13e4. Microsatellite appears set in a ministallite like repeat area. Too difficult to design primers. Bases 170 to 181 show homology to 24e5 bases 107 to 118 (12 in longest run, maxScore = 37).
PCR Comments :
Length of sequence : 309 Consensus sequence is :
..
GGTGTTTGTG TTTGTCGGTG CCCCCAGTTG CAAGTCGACC ATCGACTTGC
AACTAGGTGG TGTATGTGTT CGTTGAGGAT CCCCAGTTGC ATGTCAACCA
TCGACTCCCA ACTAGTGTGT GTGTGTGTGT GTGTGTGTTT GTCGAAGCCC
TCGGTTGCAA GGCAACTAGC GACTCGCAAC TAGGGGTCTA TGTGTGTTTG
TCGAGGGTCC CCAGTTGCAA GCCGAACATC GACTCACAAC TAGGGGATGT
GTGTGTTTAT CGAGGCCCCT AGCTACAAGT CCATCCTTGA CTCGCCAGTG
GTATCGTAC
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wmc(8e3)
Plate reference : 8e3
WMC locus number : WMC Length of sequence : 6
Last updated : Mon, Oct 13, 1997 at 2:03 pm
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : No sequence 15.9.97.
PCR Comments :
No sequence available for this clone
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wmc(8f3)
Plate reference : 8f3
WMC locus number : WMC Length of sequence : 246
Last updated : Mar 30 mai 2000 at 9:54
Owner : Rouse, Novartis Date at Agrogene : 15.9.97 Public Domain : Yes
Comments : Contains (TG)33. Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)33 85 to 150. Duplicate of 2a10 (see 2a10 for primers). Bases 123 to 141 show homology to 26f6.2 bases 186 to 204 (19 in longest run, maxScore = 43).
PCR Comments :
Length of sequence : 246 Consensus sequence is :
..
CACACGAGTT TTACGAGTTC AGGCCCTTCG CGGAGGAAGT AATAGCCCCA
CGTCTCGGAG CCCGGAGGTG GTCGACTGAA TTATGTGTGT GTGTGTGTGT
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT
TACAGGGGGT GCGAACCCTT GTGCCAGTGG AGGGGGTGGC TTATATAGAG
TGCGCCAGGA CCCCAGCCAG CCCACGTTAC AAGGAGTTCA ATGTAC
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