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Title Microbial removal of organic sulfur from coal (bacterial degradation of sulfur-containing heterocyclic compounds)
Creator/Author Klubek, B.
Publication Date1990 Mar 01
OSTI IdentifierOSTI ID: 7019091; Legacy ID: DE90010588
Report Number(s)DOE/PC/89904-T7
DOE Contract NumberFC22-89PC89904
Other Number(s)Other: ON: DE90010588
Resource TypeTechnical Report
Specific TypeProgress Report
Research OrgSouthern Illinois Univ., Carbondale, IL (USA). Dept. of Plant and Soil Science
Sponsoring OrgDOE/FE
Subject01 COAL, LIGNITE, AND PEAT; 59 BASIC BIOLOGICAL SCIENCES; COAL; DESULFURIZATION; THIOPHENE; BIODEGRADATION; AERATION; DNA; ESCHERICHIA COLI; MUTATIONS; PH VALUE; PLASMIDS; PROGRESS REPORT; SCREENING; SULFATES; TEMPERATURE EFFECTS; BACTERIA; CARBONACEOUS MATERIALS; CELL CONSTITUENTS; CHEMICAL REACTIONS; DECOMPOSITION; DOCUMENT TYPES; ENERGY SOURCES; FOSSIL FUELS; FUELS; HETEROCYCLIC COMPOUNDS; MATERIALS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; OXYGEN COMPOUNDS; SULFUR COMPOUNDS
Description/Abstract The presence of substantial levels of sulfur in coal is a major source of air pollution, and considerable efforts are being made to devise a cost-effective way of removing the sulfur. One method is to mutate a laboratory species, Escherichia coli, an organism which is genetically well-understood and whose pathways for the metabolism of sulfur-containing amino acids have been extensively investigated. Such thiophene degraders can be genetically analyzed and the genes involved can be cloned in order to amplify their products. A second approach is the development of naturally occurring bacteria capable of thiophene desulfurization. Characterization of the degradation of model compounds, enhancement of the desulfurization potential of the isolated strains via mutagenesis, and studies with crushed coal will comprise the approach used in this study. The screening of soil isolates for the potential to desulfurize thiophenic and other sources of organic sulfur will identify the best strains for the microbial removal of organic sulfur from coal. Ultimately, the genes responsible for thiophene degradation by the isolated strains will be transferred to an E. coli strain, creating a single organism capable of degrading a broad spectrum of thiophene compounds. 24 refs., 3 figs., 2 tabs.
Country of PublicationUnited States
LanguageEnglish
FormatSize: Pages: (19 p)
AvailabilityNTIS, PC A03/MF A01 - OSTI; GPO Dep.
System Entry Date2007 Feb 06

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