| Proc Natl Acad Sci U S A. 1974 December; 71(12): 4787–4791. | PMCID: PMC433982 |
Isolation of DNA-Membrane Complex in Bacillus subtilis Noboru Sueoka and Joan M. Hammers Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder Colo. 80302 Abstract A DNA-membrane complex of Bacillus subtilis was separated from the bulk of the membrane by CsCl-sucrose double gradient centrifugation. The complex has a CsCl-sucrose buoyant density of 1.35-1.45 g/cm3. Gel electrophoretic analyses show that the complex has about 5% of the whole membrane proteins and contains proteins unique to the complex as well as a small amount of membrane proteins. DNA in the complex is enriched for a genetic marker close to the origin (pur A16) and for one near the terminus (metB5). Artifactual formation of the complex during the lysate preparation was shown to be unlikely. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. Images in this article Click on the image to see a larger version. These references are in PubMed. This may not be the complete list of references from this article. - RYTER, A; JACOB, F. ETUDE AU MICROSCOPE 'ELECTRONIQUE DE LA LIAISON ENTRE NOYAU ET M'ESOSOME CHEZ BACILLUS SUBTILIS. Ann Inst Pasteur (Paris). 1964 Sep;107:384–400. [PubMed]
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