* Senior Investigator: Howard Jaffe

* Affiliation: The LNC-NINDS Protein/Peptide Sequencing Facility, Laboratory of Neurochemistry, NINDS.

* Research: Amino acid sequencing, protein chemistry,and HPLC and FPLC of proteins and peptides.

* The LNC-NINDS Protein/Peptide Sequencing Facility provides NINDS investigators with theoretical and technical expertise for the separation, purification, and amino acid sequencing of peptides and proteins. The Facility has two functions: (1) As a service mode to provide amino acid sequencing of purified proteins/peptides and (2) As a research entity to provide theoretical and practical expertise with respect to protein/peptide purification and more complicated sequencing strategies. These are done on a collaborative basis, often requiring development of new methods, but routinely include chemical and enzymatic cleavage of proteins and peptides to generate internal peptide fragments for sequence analysis, and purification of proteins and peptides by a variety of HPLC, FPLC, and electrophoretic techniques.

* Personnel/Resources: W.Timothy Link (protein chemistry, FPLC, HPLC).

The Facility is well equipped for amino acid sequencing, protein chemistry, and HPLC and FPLC of proteins and peptides. Major equipment includes: (1) Applied Biosystems Model 477A Protein Sequencer with on-line Model 120A PTH analyzer and 610 data system, (2) Beckman System Gold HPLC Systems; System A: Model 126 solvent module, Model 507 autosampler, Model 168 diode array detector, Isco Foxy II fraction collector; System B: Model 126 solvent module, Model 210 manual injector, Model 166 W detector, Model 406 analog interface module, Model 170 radioisotope detector, Isco Foxy II fraction collector, (3) Pharmacia FPLC with 2 Model P-SOOA pumps, Model GP250 gradient programmer, Model UV-M monitor, Model P-l peristaltic pump, Model Frac-100 fraction collector, (4) Waters Model 650 advanced protein purification system (FPLC) with Model 650 system controller, Model 650 pump, Model 650 injector, 484 UV detector and (5) Applied Biosystems Model 230A high performance electrophoretic chromatography (HPEC) system.

* Publications:

J. Way, M.R. Hellmich, H. Jaffe, B. Szaro, H. Pant, H. Gainer, & J. Battey (1992). Proc. Natl. Acad. Sci. USA 89:6963-7. A high molecular weight squid neurofilament protein contains a lamin-like rod domain and tail domain with lysine-serine repeats.

KT. Shetty, W.T. Link, & H.C. Pant (1993). Proc. Natl. Acad. Sci. USA (in press). CDC2-like kinase from rat spinal cord specifically phosphorylates KSPXK motifs in neurofilament proteins: Isolation and characterization.

* Contact: Dr. H. Jaffe, Bldg. 36, Room 4D20. NIH, NINDS, LNC, Bethesda, MD 20892.

Tel: (301) 402-1690 Fax: (301) 496-1339