Hoshino H, Tokizawa S, Shimizu N, Liu HY, Fang D, Haraguchi Y, Oite T; International Conference on AIDS.
Int Conf AIDS. 2000 Jul 9-14; 13: abstract no. TuPpA1218.
H. Hoshino, Gunma University, Japan, Tel.: 81-27-220-8001, Fax: 81-27-220-8006, E-mail: hoshino@akagi.sb.gunma-u.ac.jp
Background: Mesangial cells are an important component of the glomerulus. Dysfunction of mesangial cells is thought to be involved in the development of human immunodeficiency virus type 1 (HIV-1)-associated nephropathy (HIVAN). HIVAN is a structural renal failure frequently observed in patients with acquired immune deficiency syndrome. However, the susceptibility of mesangial cells to HIV-1 is disputable. More than ten G protein-coupled receptors, including chemokine receptors, have been shown to act as HIV-1 coreceptors that determine the susceptibilities of cells to HIV-1 strains with specific cell tropisms. Methods: We examined the susceptibility of mesangial cells to various HIV-1, HIV type 2(HIV-2) and simian immunodeficiency virus(SIV) strains. Expression of CD4 and HIV/SIV coreceptors was examined by Western blotting and polymerase chain reaction. Results: Mesangial cells were found to be susceptible to HIV-1 variant and mutants that infect brain-derived cells but highly resistant to T-tropic (X4), M-tropic (R5) or dual-tropic (X4R5) HIV-1 strains. In addition, mesangial cells were also susceptible to HIV-2 and SIV strains that infect the brain-derived cells. Among HIV/SIV coreceptors we tested, the expression of GPR1 mRNA was detected in mesangial cells. Expression of CD4 mRNA and protein was also detected in them. Mesangial cells and GPR1-transduced CD4-positive cells showed similar susceptibilities to the HIV-1 variant and mutants and HIV-2 and SIV strains. Conclusions: CD4 and GPR1 mRNAs were detected in mesangial cells. Mesangial cells were susceptible to HIV/SIV strains that use GPR1 as a coreceptor. Our findings suggest that an orphan G protein-coupled receptor, GPR1, is a coreceptor expressed in mesangial cells. It remains to be investigated whether the interaction of mesangial cells with specific HIV-1 strains through GPR1 plays a role in the development of HIVAN.
Publication Types:
Keywords:
- AIDS Vaccines
- AIDS-Associated Nephropathy
- Acquired Immunodeficiency Syndrome
- Antigens, CD4
- Communicable Diseases
- Connective Tissue Cells
- GPRC5C protein, human
- Glomerular Mesangium
- HIV Infections
- HIV Seropositivity
- HIV-1
- HIV-2
- Humans
- Infection
- Receptors, Chemokine
- Receptors, G-Protein-Coupled
- Simian immunodeficiency virus
- immunology
Other ID:
UI: 102238826
From Meeting Abstracts