Technical Abstract: The Agricultural Research Service of the United States Department of Agriculture and the North Carolina Agricultural Research Service announce the release of a new soybean [Glycine max L. Merr.] variety Nitrasoy. Nitrasoy is a nonnodulating group VI maturity soybean variety with a large requirement for soil-applied nitrogen to obtain excellent seed yields. It is the only public release of a nonnodulating soybean variety. Nitrasoy provides a leguminous crop option for land application of animal waste previously unavailable using commercial soybean varieties. It is adapted to southern USA, 27° - 37° N lat. Nitrasoy is an F5 selection from the cross D68-0099/`Cook¿. D68-0099 is a backcross-derived breeding line and is the donor parent of the rj1 nonnodulating gene, which when homozygous, prevents the nodulation of the soybean root by Bradyrhizobium japonicum. Experiments with three genotypes (Cook, D68-0099, and Nitrasoy) and seven soil-applied nitrogen rates (0, 49, 99, 148, 197, 246, and 296 kg ha-1 of N as ammonium nitrate) were conducted in 2002 on the Central Crops Research Station near Clayton, NC. The seed yield of nodulating variety Cook averaged 3,561 kg ha-1 with no difference across the applied N rates. In 0 N treatment, Nitrasoy and D68-0099 had similar seed yield (1,680 kg ha-1). The seed yield of Nitrasoy increased with each level of applied N rate, yielding 3,383 kg ha-1 with 250 kg ha-1 of applied N. Experiments with four non-nodulating genotypes (D68-0099, N97-2996, Nitrasoy, and N99-3342) and three nitrogen levels applied as swine-lagoon effluent were conducted at two locations in NC in 2001. The seed yield of nitrasoy was greater than the other three genotypes. Nitrasoy recovered 17% more soil plus applied N in seed than D68-0099 (193 kg ha-1 versus 165 kg N ha-1) Thus, it is an excellent choice as an N receiver crop for fields receiving animal waste. Corn-wheat-double cropped soybean (nodulated) is a common cropping system used on fields receiving swine lagoon effluent. Substitution of nonnodulated Nitrasoy into this system for nodulated soybean varieties should improve recovery of nitrogen applied in animal waste. Nitrasoy is also a useful tool for studying effects of microbial symbiotic and asymbiotic N fixation on soybean productivity. Positive effects of symbiotic N fixation by Bradyrhizobium on soybean are well known. Effects of associative N fixation from freeliving N fixing bacteria such as Azotobacter are not known, even though they are routinely included in some soybean inoculums. Nitrasoy provides a very useful tool for studying these effects.