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Research Project:
DIETARY FACTORS EARLY IN HUMAN DEVELOPMENT: HEALTH CONSEQUENCES OF PHYTOCHEMICAL INTAKE
Location: Arkansas Children's Nutrition Center
Title: ALTERED EX VIVO PLATELET AGGREGATION (PA) AFTER CONSUMPTION OF LOWBUSH BLUEBERRIES (BB) FOR 2 WEEKS IN HUMAN PATICIPANTS
Authors
| Prior, R - USDA, ARS | | Cook, R - UNIV. OF MAINE |
Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type:
Proceedings/Symposium
Publication Acceptance Date: January 10, 2004
Publication Date: March 29, 2004
Citation: Prior, R.L., Cook, R. 2004. Altered ex vivo platelet aggregation (pa) after consumption of lowbush blueberries (bb) for 2 weeks in human paticipants. The FASEB Journal. v. 18(4):348.8.
Interpretive Summary: Phytochemical components in grapes have been shown to inhibit platelet aggregation. Blueberries contain some similar chemical components, however, it is not known whether consumption of blueberries might have similar effects. In the current study, one group of 6 women consumed 1 cup of blueberries for 2 weeks while the control group did not consume blueberries. Platelet aggregation was studied before and 4 h after a meal containing carbohydrate, fat and protein either with or without a blueberry powder. Platelet aggregation was stimulated in vitro by collagen. Platelet aggregation was increased by increased collagen concentrations. Blueberry consumption inhibited platelet aggregation at the lowest collagen concentration. Consumption of 1 cup of blueberries per day appears to have a mild inhibitory effect on platelet aggregation which may be beneficial in cardiovascular events such as atherosclerosis or stroke.
Technical Abstract: Six women (42.6±4.5 y; 82.7±15.5 kg BW) (randomly assigned) consumed either 0 or 1 cup/d of frozen BB for 2 weeks. On the last day of the treatment (Trt) period, participants came into a medical center and were given a breakfast drink [Control (C) or BB] corresponding to Trt consumed during period and blood was collected during the next 4 h. Following a 2 wk washout, participants were placed on the opposite Trt for 2 weeks and the appropriate meal given. The C and BB breakfast meal provided 478 Cal, 94.5 g carbohydrate, 5.4 g fat and 11.0 g protein. In the BB meal, calories, fat and protein were removed to account for that in the BB powder. The freeze dried BB powder was 20.3% of the drink and provided 1424 mg anthocyanins, 5046 mg phenolics, and 39.9 mmol Trolox Equivalents (TE) of ORAC. Whole blood ex vivo PA and ATP release (nmol) were determined at 0 (before meal) and at 4 h after the meal using collagen (1, 2 or 5 µg/mL)(COL) as an agonist. Platelet ATP release increased in subjects that consumed BB compared to C (p<0.001). Increasing COL concentrations increased ATP release (p<0.001), but the interaction of COL by Trt was not significant. PA increased with increasing COL (p<0.001), but was not affected by sampling time (0 vs 4 h). An interaction of Trt by COL (p<0.065) was observed in ex vivo PA with 1 µg/mL COL decreasing and higher COL increasing PA in BB subjects. Dietary BB may affect cardiovascular events involving PA.
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Last Modified: 03/13/2009
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