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Title: | Adhesion and Control of Human Pathogens to and on Surfaces of Poultry Fruits and Vegetables | ||
Objective: |
FY01 Program Increase $359,190. Add 1 SY. | ||
More Info: | Model systems for characterizing the attachment and survival of Campylobacter jejuni, E.coli O157:H7, Salmonella and Listeria monocytogenes to poultry, fruit and vegetable surfaces will be developed. Mechanisms of attachment will be determined using biochemical, microbiological, immunochemical and molecular biological approaches. Methods for isolating and identifying multiple pathogens in foods or food related environments will be developed. Anti-pathogen monoclonal antibodies will be produced to detect and monitor pathogens in and on foods, and as inhibitors to detect attachment factors. Characterization of the mechanisms of attachment will provide information for developing new strategies to interfere with attachment. Plant extracts will be screened for compounds involved in attachment and that decrease the survival or virulence of pathogens on food products. Methods for measuring the types and viability of pathogens on food surfaces treated with candidate inhibitors or antimicrobials will be developed, including culture enrichment, immuno-capture, and cell sorting. | ||
Agency: | United States Department of Agriculture (USDA) Agricultural Research Service (ARS) | ||
Type: | Appropriated | ||
Start Date: | 1996 | End Date: | 2001 |
Project Number: | 5325-42000-022-00D | ||
Accession Number: | 0401009 | ||
Keywords: | food pathogens; poultry; fruit (food); vegetables; Escherichia coli; Salmonella; Listeria; antimicrobial properties; Campylobacter; plant-based foods; food surfaces; biofilm; air; water; microbial survival | ||
Institution: | USDA/ARS - Pacific West Area | ||
Investigator(s): | Robert Mandrell (Primary) Michael Cooley | ||
Food Safety Categories: | Sanitation and Pathogen Control Methodology and Quality Standards Pathogen Biology | ||
Publications: | Kimura, R., Mandrell, R.E., Galland, J.C., Hyatt, D., Riley, L.W. Restriction-site-specific PCR as a rapid test to detect enterohemorrhagic Escherichia coli O157:H7 strains in environmental samples. Applied Environmental Microbiology 2000; v. 66(6). p.2513-9.
Barak, J. D. and Gilbertson, R. L. Genetic diversity of Xanthomonas campestris pv. vitians, the causal agent of bacterial leafspot of lettuce. 2001 Phytopathology. In press. Brandl, M.T., Quinones, B., Lindow, S.E. Heterogeneous transcription of an indoleacetic acid biosynthetic gene in Erwinia herbicola on plant surfaces. PNAS (USA). 2001. 98(6). 3454-9. Barak, J.D., Koike, S.T., Gilbertson, R.L. Role of crop debris and weeds in the epidemiology of bacterial leaf spot of lettuce, caused by Xanthomonas campestris pv.vitians, in California. Plant Disease. 2001 v.85. p. 169-178. Campbell, J., Mohle-Boetani, J., Reporter, R., Abbott, S., Farrar, J., Brandl, M., Mandrell, R., Werner, S. An outbreak of Salmonella Thompson associated with fresh cilantro. Journal of Infectious Diseases. 2001. v. 183 p. 984-987. Charkowski, A.O., Sarreal, C.Z., Mandrell, R.E. Wrinkled alfalfa seeds harbor more aerobic bacteria and are more difficult to sanitize than smooth seeds. Journal of Food Protection. 2001. v. 64 (9). p. 1292-1298. Friedman, M., Brandon, D.L. Nutritional health benefits of soy proteins. Journal of Agricultural and Food Chemistry. Mar. 2001. v. 49(3). p. 1069-86. Friedman, M. Application of the S-pyridylethylation reaction to the elucidation of the structures and functions of proteins. Journal Protein Chemistry. 2001. v. 20. p. 471-493. Hallmann J., Quadt-Hallmann, A., Miller, W.G., Sikora, R.A., Lindow, S.E. Endophytic colonization of plants by the biocontrol agent Rhizobium etli G12 in relation to Meloidogyne incognita infection. 2001. Phytopathology. V. 91. p415-422. Kozukue, N., Tsuchida, H. and Friedman, M. Tracer studies on the incorporation of DL-mevalonate-2-14C into chlorophyll a and b, (-chaconine and (-solanine of potato sprouts. Journal Agricultural Food Chemistry. 2001. v. 49. p. 92-97. Miller, WG., Bates, A.H., Horn, S.T., Brandl, M.T., Quinones, B., Lindow, S.E., Biological sensor for sucrose availability: relative sensitivities of various reporter genes. Applied and Environmental Microbiology. Mar 2001. v. 67 (3). p. 1308-17. Miller, W.G., Bates, A.H., Horn, S.T., Brandl, M.T., Wachtel, M.R., Mandrell, R.E., Detection on surfaces and in Caco-2 cells of Campylobacter jejuni cells transformed with gfp, yfp and cfp marker plasmids, Applied and Environmental Microbiology. Dec. 2000. v 66(12). p. 5426-36. Miller, W.G., Leveau, J.H.L, Lindow, S.E. Improved gfp and inaZ broad-host-range promoter-probe vectors. Molecular Plant-Microbe Interactions. 2000. v. 13. p. 1243-1250 Miller, W.G., Brandl, M.T., Quinones, B., Lindow, S.E. Biological sensor for sucrose availability: relative sensitivities of various reporter genes. 2001. Applied and Environmental Microbiology. v. 67. p. 1308-1317. Wachtel, M.R., Whitehand, L.C., Mandrell, R.E. Association of Escherichia coli O157:H7 with pre-harvest leaf lettuce upon exposure to contaminated irrigation water. Journal of Food Protection. 2002. v. 65 (1). In press. Wachtel, M.R., Whitehand, L.C., Mandrell, R.E. Prevalence of Esherichia coli associated with a cabbage crop irrigated inadvertently with partially treated sewage waste water. Journal of Food Protection. 2002. In press. Alfano, J.R., Charkowski A.O., Deng W.L., Badel J.L., Petnicki-Ocwieja, T., van Dijk, K., Collmer, A. The Pseudomonas syringae Hrp pathogenicity island has a tripartite mosaic structure composed of a cluster of type III secretion genes bounded by exchangeable effector and conserved effector loci that contribute to parasitic fitness and pathogenicity in plants. Proceedings of the National Academy of Science USA. 2000. v. 97(9). p.4856-61. Collmer, A., Badel J.L., Charkowski, A.O., Deng, W.L., Fouts, D.E., Ramos, A.R., Rehm, A. H., Anderson, D.M., Schneewind, O., van Dijk, K. Pseudomonas syringae Hrp type III secretion system and effector proteins. Proceedings of the National Academy of Science USA 2000.v. 97(16).p.8770-8777. Licking, E., Gorski, L., Kaiser, D. A common step for changing cell shape in fruiting body and starvation-independent sporulation of Myxococcus xanthus. Journal of Bacteriology; v. 182. p.3553-3558. Gorski, L., Gornewold, T., Kaiser, D. A sigma-54 activator protein necessary for spore differentiation within the fruiting body of Myxococcus xanthus. Journal of Bacteriology. 2000. v.182. p.2438-2444. Cooley, M.B., Pathirana, S., Wu, H.J., Kachroo, P., Klessig, D.F. Members of the Arabidopsis HRT/RPP8 family of resistance genes confer resistance to both viral and oomycete pathogens. Plant Cell. 2000. v.12. p.663-676. Miller, W.G., Bates, A.H., T, H.S., Brandl, M.T., Wachtel, M.R., and Mandrell, R.E. 2000. Detection of Campylobacter jejuni cells transformed with new gfp, yfp and cfp marker plasmids on surfaces and in Caco-2 cells Applied and Environmental Microbioogy. V.66 (12). In press | ||
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