|
Cold Spring Harbor Laboratory Course: This course provides extensive laboratory exposure to the biology, manipulation and use of oocytes and embryos of Xenopus. The course consists of intensive laboratory sessions, supplemented by daily lectures and demonstrations from experts in cellular, experimental and molecular development. Areas to be covered include: (i) care of adults; (ii) oocyte isolation and embryo production; (iii) stages of embryonic development and anatomy; (iv) whole mount in situ hybridization and immunocytochemistry; (v) microinjection of eggs and oocytes with lineage tracers, DNA constructs, mRNA and antisense oligonucleotides; (vi) micromanipulation of embryos, including explant and transplantation assays; (vii) preparation of transgenic embryos; and (viii) use of Xenopus tropicalis for genetic analyses. This course is suited both for investigators who have had no experience with Xenopus, as well as those who have worked with Xenopus and wish to learn new and cutting edge techniques. All applicants should have current training in molecular biology and some knowledge of developmental biology. For more information, see http://meetings.cshl.org/2004/2004c-xeno.htm. |
