RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Albacore ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Thunnus alalunga
FDA Seafood List Common Name: Albacore
FDA Seafood List Market Name: Tuna
Unique IEF Gel Code: 19960313gel01
Unique Web Code: alg01Srr
Tissue Aliquot Code: thunalal A96-001 #05a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 12
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	47	4.1	0.410378	17	12.1323	25.4	18.6	3.5	50.2	17.2	0.398956
2	4.55	189	16.6	0.38144	5	3.0727	6.4	4.7	4.55	189.6	4.7	0.364976
3	5.2	271	23.8	0.334166	5	2.6473	5.5	4.1	5.2	270.3	4.5	0.33062
4	5.85	427	37.6	0.8254	3	4.82854	10.1	7.4	5.85	426.7	3.2	0.84028
5	6.55	611	53.7	0.417174	4	2.64557	5.5	4.1	6.55	610.4	3.7	0.399377
6	6.85	653	57.4	0.250759	7	2.55865	5.4	3.9	6.85	652	6.5	0.220761
7	7.35	750	66	0.682391	7	7.55944	15.8	11.6	7.35	749	6.7	0.632717
8	8.15	911	80.1	0.155213	8	2.09459	4.4	3.2	8.15	910.2	7.9	0.149934
9	8.45	964	84.8	0.276754	6	2.79592	5.9	4.3	8.45	963.9	5.9	0.265462
10	8.65	1000	88	0.246018	5	2.27908	4.8	3.5	8.65	999.5	5.5	0.235253
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1116	98.2	0.555624	6	5.11578	10.7	7.8	9.3	1115.1	5.6	0.517648

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.665	72	6.3	0.063661	7	0.867645	1.6	1	3.665	70.5	7.1	0.0694072
2	5.621	379	33.3	0.78453	4	5.47167	10.1	6.4	5.621	378.8	3.9	0.801175
3	5.696	397	34.9	0.161675	3	0.850223	1.6	1	5.696	396.7	3	0.161205
4	6.216	528	46.4	0.0809523	4	0.60339	1.1	0.7	6.216	527	4.3	0.0793766
5	6.403	576	50.7	0.0572731	4	0.332748	0.6	0.4	6.403	575.8	3.7	0.0506155
6	6.586	619	54.4	1.03594	6	11.9219	22	14	6.586	616.2	6.4	1.04559
7	6.731	639	56.2	0.0604247	3	0.763114	1.4	0.9	6.731	642.7	3.4	0.124824
8	6.817	651	57.3	0.300299	4	2.24556	4.2	2.6	6.817	650.7	4.1	0.307814
9	6.931	671	59	0.113143	4	0.831073	1.5	1	6.931	671.8	4.1	0.114053
10	7.129	709	62.4	0.204404	4	1.5504	2.9	1.8	7.129	709.1	4.3	0.201818
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.202	723	63.6	1.02062	4	8.67414	16	10.2	7.202	723.8	4.5	1.09652
12	7.347	751	66.1	0.144036	18	4.16085	7.7	4.9	7.347	766.8	18	0.13042
13	7.492	780	68.6	0.550212	4	3.43693	6.4	4	7.492	779.6	4.2	0.465927
14	7.611	804	70.7	0.915343	3	4.93785	9.1	5.8	7.611	803.8	3	0.931384
15	8.43	962	84.6	0.391883	4	2.88243	5.3	3.4	8.43	962.2	4	0.404561
16	9.028	1069	94	0.295349	9	4.5449	8.4	5.3	9.028	1068.1	8.7	0.295553

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	48	4.2	0.551679	16	14.8626	27.2	19.4	3.5	50.8	16.2	0.516113
2	4.55	199	17.5	0.579975	4	4.01892	7.4	5.2	4.55	199.5	4.3	0.529339
3	5.2	284	25	0.539459	4	3.47876	6.4	4.5	5.2	284.2	3.8	0.519894
4	5.85	437	38.5	0.896627	3	5.33325	9.8	7	5.85	437.4	3.2	0.928515
5	6.55	614	54.1	0.441989	4	2.7246	5	3.6	6.55	613.6	3.7	0.418824
6	6.85	655	57.8	0.270056	6	2.62216	4.8	3.4	6.85	654.6	6.4	0.232474
7	7.35	750	66.1	0.679599	7	7.38924	13.5	9.7	7.35	749	6.7	0.625248
8	8.15	911	80.3	0.182225	8	2.55577	4.7	3.3	8.15	910.5	8	0.181017
9	8.45	964	85	0.313996	6	3.14631	5.8	4.1	8.45	964.3	5.7	0.309561
10	8.65	1000	88.2	0.302396	5	2.45514	4.5	3.2	8.65	1000.4	4.7	0.297886
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1116	98.4	0.668107	5	6.02827	11	7.9	9.3	1115.1	5.5	0.620786

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	52	4.6	0.126914	10	2.68304	2.8	2.3	3.5	57	9.7	0.156373
2	3.649	72	6.3	0.298462	11	5.32612	5.6	4.6	3.649	72.4	10.5	0.284911
3	4.55	193	17	0.349028	4	2.53414	2.7	2.2	4.55	193.3	4.4	0.322509
4	5.2	272	24	0.289993	5	2.44984	2.6	2.1	5.2	271.4	5.5	0.251987
5	5.597	368	32.5	0.769274	4	5.76539	6.1	5	5.597	367.7	4.2	0.781419
6	5.664	384	33.9	0.150502	3	1.02088	1.1	0.9	5.664	382.8	3.5	0.166105
7	5.85	429	37.8	0.498905	3	2.74898	2.9	2.4	5.85	428.9	3.1	0.503981
8	6.198	522	46	0.100593	3	0.455297	0.5	0.4	6.198	522.1	2.6	0.0998716
9	6.55	616	54.3	1.06099	6	10.6872	11.3	9.3	6.55	613.2	6.5	0.931364
10	6.768	640	56.4	0.123755	2	0.389938	0.4	0.3	6.768	638.8	2.5	0.0896938
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.85	649	57.2	0.238683	7	2.86505	3	2.5	6.85	648.4	6.9	0.233049
12	7.159	707	62.3	0.139268	4	0.870251	0.9	0.8	7.159	707.3	3.6	0.136215
13	7.265	727	64.1	0.984773	4	7.26499	7.7	6.3	7.265	727.6	4	1.03363
14	7.35	743	65.5	0.523535	4	3.65585	3.9	3.2	7.35	742.5	3.9	0.526029
15	7.405	754	66.5	0.0758696	2	0.217514	0.2	0.2	7.405	754.1	1.8	0.0674871
16	7.555	784	69.1	0.480875	5	3.89499	4.1	3.4	7.555	783.6	4.6	0.473478
17	7.665	806	71.1	0.8464	3	5.17904	5.5	4.5	7.665	805.3	3.3	0.876534
18	8.15	903	79.6	0.184426	11	3.30845	3.5	2.9	8.15	904.8	10.6	0.176759
19	8.45	966	85.2	0.624295	4	3.76799	4	3.3	8.45	965.4	4.5	0.476937
20	8.65	1000	88.2	0.353261	24	16.1167	17	14	8.65	987.5	23.8	0.382517
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	9.006	1064	93.8	0.295625	12	5.84955	6.2	5.1	9.006	1062.6	12.4	0.265643
22	9.3	1117	98.5	0.767396	6	7.52146	8	6.5	9.3	1115.3	6.1	0.697791

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.612	72	6.3	0.0675266	6	0.788843	1.5	1	3.612	71.3	6.3	0.0705345
2	5.61	374	33	0.773585	4	5.64081	10.9	7.1	5.61	374.1	4	0.792434
3	5.676	390	34.4	0.158071	3	0.899118	1.7	1.1	5.676	389.8	3.2	0.15733
4	6.195	522	46	0.0831288	3	0.367865	0.7	0.5	6.195	521.8	2.5	0.0819831
5	6.373	568	50.1	0.0719317	7	0.772663	1.5	1	6.373	566.2	6.7	0.0648457
6	6.419	580	51.1	0.0496934	4	0.337787	0.7	0.4	6.419	579.1	4.4	0.0431414
7	6.538	611	53.9	1.05731	6	10.4657	20.1	13.2	6.538	609.4	6	0.990187
8	6.79	644	56.8	0.207049	8	2.58561	5	3.3	6.79	643.4	8.4	0.174257
9	7.116	702	61.9	0.192987	4	1.13655	2.2	1.4	7.116	702	3.6	0.176969
10	7.237	725	63.9	1.02566	4	7.90342	15.2	10	7.237	725.6	4.4	1.01584
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.347	746	65.8	0.135296	6	1.12206	2.2	1.4	7.347	747.7	5.9	0.107782
12	7.496	776	68.4	0.573994	5	5.14234	9.9	6.5	7.496	775.4	5.5	0.530858
13	7.604	798	70.4	0.913914	3	5.64888	10.9	7.1	7.604	797.2	3.3	0.956991
14	8.4	956	84.3	0.353198	4	2.69277	5.2	3.4	8.4	956.6	4.1	0.37388
15	9.012	1066	94	0.300689	13	6.441	12.4	8.1	9.012	1063.5	13.1	0.277826

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	63	5.6	0.449882	30	21.1156	33.1	25.8	3.5	60.5	29.9	0.398247
2	4.55	195	17.2	0.48301	4	3.06422	4.8	3.7	4.55	195.5	3.9	0.447482
3	5.2	276	24.3	0.464285	4	2.95638	4.6	3.6	5.2	276	3.7	0.448978
4	5.85	436	38.4	0.835144	3	5.20502	8.2	6.4	5.85	435.5	3.4	0.875388
5	6.55	612	54	0.364011	4	2.42601	3.8	3	6.55	611.4	4	0.343905
6	6.85	654	57.7	0.264734	7	2.92936	4.6	3.6	6.85	653.7	7.4	0.223332
7	7.35	750	66.1	0.666862	6	7.10574	11.1	8.7	7.35	749.4	6.4	0.623767
8	8.15	914	80.6	0.216095	8	2.97967	4.7	3.6	8.15	913	8	0.209765
9	8.45	965	85.1	0.418631	6	4.33517	6.8	5.3	8.45	964.7	6.1	0.400308
10	8.65	1001	88.3	0.325766	6	3.34057	5.2	4.1	8.65	1000.3	6	0.311734
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1119	98.7	0.810674	6	8.30839	13	10.1	9.3	1117	6.2	0.752369

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 86

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 25.400 4.550 6.400 5.200 5.500 5.850 10.100 6.550 5.500 6.850 5.400 7.350 15.800 8.150 4.400 8.450 5.900 8.650 4.800 9.300 10.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 12 PeakCount: 11 Return to Lane Navigator
3.665 1.600 5.621 10.100 5.696 1.600 6.216 1.100 6.403 0.600 6.586 22.000 6.731 1.400 6.817 4.200 6.931 1.500 7.129 2.900 7.202 16.000 7.347 7.700 7.492 6.400 7.611 9.100 8.430 5.300 9.028 8.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 13 PeakCount: 16 Return to Lane Navigator
3.500 27.200 4.550 7.400 5.200 6.400 5.850 9.800 6.550 5.000 6.850 4.800 7.350 13.500 8.150 4.700 8.450 5.800 8.650 4.500 9.300 11.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 14 PeakCount: 11 Return to Lane Navigator
3.500 2.800 3.649 5.600 4.550 2.700 5.200 2.600 5.597 6.100 5.664 1.100 5.850 2.900 6.198 0.500 6.550 11.300 6.768 0.400 6.850 3.000 7.159 0.900 7.265 7.700 7.350 3.900 7.405 0.200 7.555 4.100 7.665 5.500 8.150 3.500 8.450 4.000 8.650 17.000 9.006 6.200 9.300 8.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 15 PeakCount: 22 Return to Lane Navigator
3.612 1.500 5.610 10.900 5.676 1.700 6.195 0.700 6.373 1.500 6.419 0.700 6.538 20.100 6.790 5.000 7.116 2.200 7.237 15.200 7.347 2.200 7.496 9.900 7.604 10.900 8.400 5.200 9.012 12.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 16 PeakCount: 15 Return to Lane Navigator
3.500 33.100 4.550 4.800 5.200 4.600 5.850 8.200 6.550 3.800 6.850 4.600 7.350 11.100 8.150 4.700 8.450 6.800 8.650 5.200 9.300 13.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 17 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Albacore ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Albacore ---- Go to Display Gel Lane Navigator.