Voth R, Rossol S, Klein K, Hess G, Schutt K, Schroder HC, Meyer zum Buschenfelde KH, Muller WE; International Conference on AIDS.
Int Conf AIDS. 1990 Jun 20-23; 6: 162 (abstract no. S.A.321).
Med Klinik and Poliklinik, Johannes-Gutenberg Universitat, Mainz, FRG
OBJECTIVE: Human PBMC from HIV-1 infected individuals produced ex vivo in response to vesicular stomatitis virus (VSV) only low amounts of IFN-alpha. In order to define if the decrease of the level of transcripts is also observed for TNF-alpha, transcriptional regulation of TNF-alpha was studied in HIV-1 infected patients at various stages of disease. METHODS: PBMC from 142 HIV-1 infected individuals were stimulated with LPS for TNF-alpha production. Northern blot analysis was used to define TNF-alpha transcripts and for detection of the protein a TNF-alpha ELISA was conducted. RESULTS: Almost parallel with the increase of the level of the transcripts for TNF-alpha, the level of TNF-beta increases as well. Data are presented which show that the increased TNF-alpha production is due to a longer half-life of TNF-alpha transcripts in PBMC from infected individuals. CONCLUSIONS: The results let us suggest that the upregulation of TNF-alpha gene expression in PBMC from HIV-infected individuals is controlled predominantly on the posttranscriptional level, whereas transcriptional events regulate the level of IFN-alpha transcripts. This assumption is supported by run-on experiments which revealed that the extent of transcription of TNF-alpha gene is almost identical in nuclei from stimulated PBMC of noninfected and HIV infected donors, whereas the transcription of IFN-alpha gene is strongly suppressed in nuclei from HIV-infected individuals at WR stages 3-6.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Blotting, Northern
- Gene Expression
- HIV
- HIV Antigens
- HIV Core Protein p24
- HIV Infections
- HIV Seropositivity
- HIV-1
- Humans
- Interferon-alpha
- Lipopolysaccharides
- Lymphotoxin-alpha
- TNF protein, human
- Transcription, Genetic
- Tumor Necrosis Factor-alpha
- genetics
- immunology
Other ID:
UI: 102196120
From Meeting Abstracts