UI  - 98003244
TI  - The influence of prostaglandin E1 on platelet adherence and injury in
      preserved rat liver allografts.
AB  - We have previously shown that part of the injury sustained by
      cold-preserved livers on reperfusion is the consequence of platelet
      adhesion to sinusoidal endothelium. The purpose of the present study
      was to determine whether prostaglandin E1 (PGE1) can reduce the
      injury and if so, how to maximize this beneficial effect. Rat livers
      were cold-preserved in University of Wisconsin solution for 30 hours
      then subjected to 1-hour warm ischemia after which they were
      reperfused at 37 degrees C with oxygenated Krebs-Henseleit solution
      with or without isolated platelets. PGE1 was used to treat the donor
      liver during harvesting, cold preservation, and reperfusion. In some
      studies, PGE1 was used to pretreat platelets before exposing them to
      the liver, and in other studies, both liver and platelets were
      treated. Pretreatment of platelets with paraformaldehyde, which
      inactivates them, or ADP, which activates them, was also studied.
      Treatment of livers with PGE1 significantly decreased preservation
      injury when livers were reperfused in the absence of platelets.
      However, when platelets were added to the perfusate, prior treatment
      of the liver with PGE1 had relatively minor beneficial effects.
      Pretreatment of platelets alone with PGE1 was also beneficial, but
      again the effect was small. However, when both liver and platelets
      were treated with PGE1 there was a highly significant decrease in the
      extent of liver injury and platelet adhesion. Perfusate transaminase
      levels were lower, bile flow was improved, and histologically, livers
      appeared less injured. Pretreatment of platelets with
      paraformaldehyde produced similar results to pretreatment with PGE1.
      When platelets were preactivated with adenosine diphosphate,
      extensive hepatic injury occurred upon reperfusion despite PGE1
      treatment of the liver. PGE1 can lessen preservation-reperfusion
      injury impressively when administered to both liver and platelets but
      has little effect when platelets have been preactivated.