Hatch WC, Burstein Y, Calvelli TA, Rashbaum WK, Lyman WD; International Conference on AIDS.
Int Conf AIDS. 1991 Jun 16-21; 7: 97 (abstract no. M.A.1020).
Albert Einstein Coll. Med., NY, NY, USA
OBJECTIVE: To compare the kinetics of HIV-1 gene expression in human fetal macrophages (HFM) infected in vivo with the kinetics of infection of normal HFM in vitro using different HIV-1 isolates. METHODS: HFM from fetal livers were obtained from abortuses of HIV-1 seropositive and seronegative women. The cells were cultured using standard conditions and analyzed for phenotype by flow cytometry and non-specific esterase staining. HFM from abortuses of HIV-1 seropositive females were monitored for infection by reverse transcriptase (RT) and p24 assays, immunocytochemistry and nucleic acid hybridization. HFM from abortuses of seronegative women were exposed to the RF (L-cytotropic) and JRFL (M-cytotropic) isolates of HIV-1. These cultures were monitored for HIV-1 infection using the same methods. RESULTS: HFM obtained from HIV-1 seropositive females showed a 3-fold increase in RT activity after 30 days in culture. Similarly, HFM from abortuses of seronegative females incubated with the RF strain showed a 3-fold increase in RT activity after 30 days in culture. In contrast, HFM incubated with the JRFL strain showed a 50-fold increase in RT activity by 14 days in culture. DISCUSSION AND CONCLUSIONS: The kinetics of HIV-1 expression in cultured HFM infected in vivo were similar to HFM infected in vitro by the L-cytotropic RF isolate of HIV-1. This slow-low expression of HIV-1 may be related to viral latency. In contrast, the expression of viral genes after exposure of HFM to the M-cytotropic isolate (JRFL) demonstrated high-fast kinetics. These data suggest that HIV-1 infection of the human fetus by distinct viral isolates may contribute to the observed differences in post-natal pathology.
Publication Types:
Keywords:
- Animals
- Culture
- Cytopathogenic Effect, Viral
- Female
- Fetus
- Gene Expression
- Genes, Viral
- HIV Infections
- HIV Seropositivity
- HIV-1
- Humans
- In Vitro
- Laboratory Techniques and Procedures
- Macrophages
- RNA-Directed DNA Polymerase
- genetics
- organization & administration
- virology
Other ID:
UI: 102182584
From Meeting Abstracts