| J Clin Microbiol. 1991 September; 29(9): 1959–1962. | PMCID: PMC270242 |
Direct detection of molluscum contagiosum virus in clinical specimens by dot blot hybridization. J W Hurst, B Forghani, C S Chan, L Oshiro, and G Darai Viral and Rickettsial Disease Laboratory, California State Department of Health Services, Berkeley 94704. Abstract A dot blot hybridization protocol was developed for the direct detection of molluscum contagiosum virus (MCV) DNA in clinical specimens submitted for virus isolation. Samples were concentrated by high-speed centrifugation and treated with proteinase K; this was followed by a single phenol-chloroform extraction step. The DNA was denatured, and the entire volume was spotted onto a nitrocellulose membrane. A biotinylated DNA probe specific for the BamHI-C region of MCV type 1 was used for hybridization. Evidence of MCV DNA was visualized by using streptavidin alkaline phosphatase conjugate and 5-bromo-4-chloro-3-indolyl phosphate-nitroblue tetrazolium as the substrate. Results showed that nonspecific hybridization does not occur with herpes simplex virus- or orf virus-infected clinical specimens and that dot blotting is more sensitive and reproducible than electron microscopy. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (839K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. Images in this article Click on the image to see a larger version. These references are in PubMed. This may not be the complete list of references from this article. - Blank, H; Davis, C; Collins, C. Electron microscopy for the diagnosis of cutaneous viral infections. Br J Dermatol. 1970;83(Suppl):69–80. [PubMed]
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