Yamamoto T, Yamamoto S, Kataoka T, Kohase M, Komuro K, Tokunaga T; International Conference on AIDS.
Int Conf AIDS. 1994 Aug 7-12; 10: 101 (abstract no. PA0286).
Natl. Inst. of Health, Tokyo, Japan.
OBJECTIVE: Interferon (IFN) plays an important role in the HIV disease. Recent studies show some effects of IFN in the treatment of early HIV-infection. Cell culture studies also support these effects. HIV infection of CD4 positive cells is blocked by IFN administered at the time of viral challenge. Here we present a paper that the ability of the synthetic single-stranded oligodeoxyribonucleotides (oligo DNAs) possessing particular hexamer palindromic sequences to induce IFN production of human peripheral blood lymphocytes (PBL). RESULTS: When PBL were cultued with a 30-mer oligo DNA with the palindrome of AACGTT, IFN was detected in the culture fluid 8 hr later and the titer of IFN reached 10(3)-10(4) units/ml at the maximal level 18 hr later. Type of IFN induced with oligo DNAs was dominantly IFN-alpha. DISCUSSION AND CONCLUSIONS: It is suggested that certain palindoromic structures in the synthetic oligo DNAs are essential to induce IFN production of PBL.
Publication Types:
Keywords:
- Base Sequence
- DNA
- HIV Infections
- Humans
- Interferon-alpha
- Interferons
- Oligodeoxyribonucleotides
- Oligonucleotides
- genetics
Other ID:
UI: 102209832
From Meeting Abstracts