BT0847

Library Description

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

48 ESTs from this library were grouped into 24 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM		UniGene Entry
5	104167		Hs.618461	MRNA; cDNA DKFZp667G1510 (from clone DKFZp667G1510).
5	104167		Hs.524571	Cell division cycle associated 8.
5	104167		Hs.491682	Protein kinase, DNA-activated, catalytic polypeptide.
5	104167		Hs.445952	Transcribed locus.
3	62500		Hs.619713	Transcribed locus.
2	41667		Hs.697098	Transcribed locus, strongly similar to XP_216331.4 PREDICTED: similar to dpy-19-like 3 [Rattus norvegicus].
2	41667		Hs.654350	GA binding protein transcription factor, beta subunit 1.
2	41667		Hs.529901	X (inactive)-specific transcript (non-protein coding).
2	41667		Hs.517065	Regulating synaptic membrane exocytosis 4.
2	41667		Hs.461086	Cadherin 1, type 1, E-cadherin (epithelial).
2	41667		Hs.446352	V-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian).
1	20833		Hs.77422	Proteolipid protein 2 (colonic epithelium-enriched).
1	20833		Hs.709730	Zinc finger protein 586.
1	20833		Hs.708095	Forkhead box K1.
1	20833		Hs.687944	Transcribed locus.
1	20833		Hs.658922	Musashi homolog 2 (Drosophila).
1	20833		Hs.632348	Solute carrier family 7 (cationic amino acid transporter, y+ system), member 8.
1	20833		Hs.567303	Mdm2 p53 binding protein homolog (mouse).
1	20833		Hs.544577	Glyceraldehyde-3-phosphate dehydrogenase.
1	20833		Hs.528664	Potassium channel, subfamily K, member 15.
1	20833		Hs.517134	Chromosome 20 open reading frame 43.
1	20833		Hs.367956	F-box and leucine-rich repeat protein 14.
1	20833		Hs.156814	Histidine acid phosphatase domain containing 2A.
1	20833		Hs.131262	Transcribed locus.

Source: NCBI UniGene

EST Sequences

97 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

• mammary gland
• breast (mammary gland) tumor
• adult
• female

Source: NCBI Clone Registry

References

Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).