RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Cusk ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Brosme brosme
FDA Seafood List Common Name: Cusk
FDA Seafood List Market Name: Cusk
Unique IEF Gel Code: 19930823gel04
Unique Web Code: ckg01Srr
Tissue Aliquot Code: brosbros 001 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 9
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	59	5.1	0.275543	8	3.87055	10.9	6.9	3.5	60.4	8.4	0.259072
2	4.55	200	17.2	0.314923	6	3.11042	8.8	5.5	4.55	200.2	6.1	0.289674
3	5.2	277	23.8	0.370297	6	3.99341	11.3	7.1	5.2	278.2	5.7	0.393205
4	5.85	427	36.7	0.581677	5	4.69467	13.3	8.3	5.85	427.3	4.6	0.577494
5	6.55	594	51	0.451057	6	4.17568	11.8	7.4	6.55	593.4	5.7	0.412517
6	6.85	635	54.6	0.213351	7	2.46428	7	4.4	6.85	634.9	7.4	0.187392
7	7.35	727	62.5	0.326477	7	3.59101	10.1	6.4	7.35	725.4	6.6	0.306454
8	8.15	889	76.4	0.0990684	8	1.31274	3.7	2.3	8.15	889.9	7.6	0.0971188
9	8.45	948	81.4	0.139054	6	1.53424	4.3	2.7	8.45	948.3	6.3	0.137872
10	8.65	988	84.9	0.0923559	6	0.910299	2.6	1.6	8.65	986.8	5.7	0.0904002
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1103	94.8	0.497359	7	5.74017	16.2	10.2	9.3	1100.7	7.1	0.45765

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 8
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 10
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	70	6	0.696985	6	7.0138	10.2	7.9	3.5	68.9	5.9	0.668415
2	4.082	146	12.5	0.497065	4	3.84087	5.6	4.3	4.082	145.8	4.3	0.500307
3	4.297	174	14.9	0.755282	4	6.07299	8.8	6.9	4.297	174.6	4.3	0.792442
4	4.443	193	16.6	0.0199369	3	0.0978892	0.1	0.1	4.443	192.6	2.6	0.0208433
5	4.55	207	17.8	0.228007	4	1.59145	2.3	1.8	4.55	207.2	4.2	0.214623
6	4.767	233	20	0.372034	4	2.94338	4.3	3.3	4.767	233.4	4.4	0.378408
7	4.942	254	21.8	0.0326058	6	0.352909	0.5	0.4	4.942	254.4	6.1	0.0324812
8	5.008	262	22.5	0.0923901	3	0.492056	0.7	0.6	5.008	261.9	3.1	0.0894981
9	5.1	273	23.4	0.089538	2	0.342303	0.5	0.4	5.1	272.9	2.4	0.0819033
10	5.2	285	24.5	0.274999	8	3.40753	4.9	3.8	5.2	286.8	7.7	0.250976
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.285	304	26.1	0.613438	3	3.28816	4.8	3.7	5.285	303.9	3.1	0.602177
12	5.427	336	28.8	0.014569	4	0.0945878	0.1	0.1	5.427	337.2	4.1	0.0131094
13	5.503	353	30.3	0.03024	3	0.176148	0.3	0.2	5.503	352.8	3.2	0.0313486
14	5.592	373	32	0.551354	4	3.57557	5.2	4	5.592	373.2	3.6	0.559392
15	5.667	390	33.5	0.417769	8	5.50728	8	6.2	5.667	391.2	7.8	0.399878
16	5.85	431	37	0.438934	4	3.17719	4.6	3.6	5.85	431.3	4.1	0.439187
17	6.115	493	42.3	0.0882787	2	0.378509	0.5	0.4	6.115	493.1	2.5	0.0864189
18	6.174	507	43.5	0.0229941	4	0.163841	0.2	0.2	6.174	506.8	4.5	0.0206362
19	6.247	524	45	0.469713	2	1.73039	2.5	2	6.247	524.1	2.4	0.406466
20	6.285	533	45.8	0.0953715	9	1.67404	2.4	1.9	6.285	529.6	8.6	0.109491
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.46	574	49.3	0.0907461	16	2.63039	3.8	3	6.46	577.2	16	0.0926157
22	6.55	595	51.1	0.233885	6	2.00946	2.9	2.3	6.55	594.1	5.6	0.203957
23	6.85	635	54.5	0.146407	8	1.76925	2.6	2	6.85	635.9	7.7	0.128996
24	7.35	726	62.3	0.444947	5	4.05307	5.9	4.6	7.35	725.5	5.5	0.418873
25	7.632	782	67.1	0.0551294	12	0.951461	1.4	1.1	7.632	776.4	11.9	0.0450823
26	7.793	814	69.9	0.0208426	11	0.366932	0.5	0.4	7.793	813.5	11	0.018737
27	8.15	885	76	0.438853	3	1.93942	2.8	2.2	8.15	885.6	2.8	0.396548
28	8.45	944	81	0.118575	7	1.41122	2	1.6	8.45	944.2	6.9	0.115358
29	8.65	987	84.7	0.117681	5	1.11799	1.6	1.3	8.65	987.1	5.5	0.115056
30	9.3	1103	94.7	0.533698	8	6.87956	10	7.8	9.3	1100.9	7.9	0.492513

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 9
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 11
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.531	73	6.3	0.696642	4	4.89369	11.7	8.9	3.531	72.9	3.9	0.710754
2	4.125	150	12.9	0.41876	4	3.28832	7.9	6	4.125	150.2	4.4	0.417931
3	4.342	178	15.3	0.754459	4	5.95377	14.3	10.8	4.342	178.5	4.3	0.7819
4	4.803	235	20.2	0.330298	5	3.104	7.4	5.7	4.803	235.3	5.3	0.33217
5	5.023	261	22.4	0.0428476	5	0.336655	0.8	0.6	5.023	260	4.6	0.0414478
6	5.175	279	23.9	0.0595694	12	1.17583	2.8	2.1	5.175	282.5	11.6	0.0572815
7	5.252	294	25.2	0.152046	4	1.01606	2.4	1.9	5.252	293.6	4.2	0.13583
8	5.3	305	26.2	0.634261	5	5.3479	12.8	9.7	5.3	305.6	4.8	0.622337
9	5.444	338	29	0.0051686	3	0.0272135	0.1	0	5.444	337.3	3.1	0.00488409
10	5.518	355	30.4	0.0193268	3	0.107576	0.3	0.2	5.518	355.8	3	0.0204002
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.562	365	31.3	0.0489917	2	0.22617	0.5	0.4	5.562	365.8	2.2	0.057421
12	5.61	376	32.2	0.54909	4	3.59635	8.6	6.5	5.61	376.4	3.7	0.55498
13	5.68	392	33.6	0.450268	7	5.33919	12.8	9.7	5.68	392.8	6.8	0.442106
14	5.875	437	37.5	0.0190153	11	0.358106	0.9	0.7	5.875	437.2	10.9	0.018581
15	6.107	492	42.2	0.102731	4	0.704277	1.7	1.3	6.107	491.8	4.4	0.0898191
16	6.17	507	43.5	0.0490962	6	0.578482	1.4	1.1	6.17	506.3	6	0.0545218
17	6.242	524	44.9	0.421112	5	3.56383	8.5	6.5	6.242	524	5.3	0.379547
18	6.44	571	49	0.0313463	11	0.623258	1.5	1.1	6.44	566.6	10.8	0.0326256
19	7.577	774	66.4	0.0416203	13	0.910152	2.2	1.7	7.577	769.7	13.2	0.0388657
20	7.741	807	69.2	0.0304184	12	0.58852	1.4	1.1	7.741	809.6	12.4	0.0266934

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 10
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 12
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	71	6.1	0.197236	7	2.4445	8.6	4.9	3.5	72.1	7.2	0.192173
2	4.55	206	17.6	0.273985	5	2.34145	8.2	4.7	4.55	206.2	5.5	0.242266
3	5.2	282	24.1	0.362079	5	2.97104	10.4	6	5.2	281.9	4.9	0.343343
4	5.85	434	37.1	0.525635	4	4.07341	14.3	8.2	5.85	433.8	4.3	0.530206
5	6.55	602	51.4	0.384586	5	3.34723	11.7	6.7	6.55	601.1	5.5	0.346408
6	6.85	642	54.8	0.177333	7	2.03668	7.1	4.1	6.85	642.5	7.4	0.156139
7	7.35	734	62.7	0.290432	7	3.37133	11.8	6.8	7.35	732.3	7.2	0.262364
8	8.15	896	76.5	0.075014	9	1.19092	4.2	2.4	8.15	896	9.3	0.0723202
9	8.45	955	81.6	0.0976577	6	0.984188	3.4	2	8.45	954.4	5.6	0.0984447
10	8.65	993	84.8	0.0689781	6	0.691879	2.4	1.4	8.65	992.3	5.7	0.0682808
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1107	94.5	0.427412	7	5.12614	17.9	10.3	9.3	1104.3	7.5	0.385742

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	76	6.5	0.652183	7	7.07901	9.7	7.9	3.5	75.5	6.5	0.612027
2	4.071	150	12.8	0.422787	5	3.43954	4.7	3.8	4.071	150.5	4.6	0.426437
3	4.295	179	15.3	0.692595	4	5.58476	7.7	6.2	4.295	179	4.4	0.714839
4	4.434	197	16.9	0.0236239	3	0.125847	0.2	0.1	4.434	197.2	2.9	0.0244159
5	4.55	212	18.1	0.192984	5	1.68581	2.3	1.9	4.55	211.8	4.9	0.19272
6	4.769	238	20.4	0.260335	6	2.79059	3.8	3.1	4.769	237.4	6.1	0.256869
7	5.116	279	23.9	0.127865	16	3.32202	4.6	3.7	5.116	269.3	15.8	0.118811
8	5.2	289	24.7	0.341601	7	3.65344	5	4.1	5.2	290.7	6.7	0.308433
9	5.283	308	26.3	0.320289	7	3.95832	5.4	4.4	5.283	306.2	7.4	0.300969
10	5.418	339	29	0.00911341	4	0.0552233	0.1	0.1	5.418	338	3.5	0.00889648
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.492	356	30.5	0	2	0.0101759	0	0	5.492	356	2	0.00287059
12	5.545	368	31.5	0.0596597	3	0.304248	0.4	0.3	5.545	368.2	2.8	0.0604531
13	5.584	377	32.2	0.541915	4	3.35416	4.6	3.7	5.584	377.2	3.7	0.51548
14	5.649	392	33.5	0.350466	10	5.91343	8.1	6.6	5.649	393	9.6	0.347748
15	5.754	416	35.6	0.0201191	7	0.00740576	0	0	5.754	417.9	6.9	0.00060649
16	5.85	438	37.5	0.458019	4	3.57354	4.9	4	5.85	438.2	4.3	0.470614
17	6.113	498	42.6	0.0219069	3	0.110818	0.2	0.1	6.113	497.9	2.9	0.0213156
18	6.169	511	43.7	0.01839	6	0.148125	0.2	0.2	6.169	511.1	5.8	0.0144756
19	6.235	526	45	0.49066	2	2.04148	2.8	2.3	6.235	526.2	2.5	0.464281
20	6.458	577	49.4	0.0849738	18	2.91489	4	3.3	6.458	582.8	18.4	0.0895036
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.55	598	51.2	0.309565	5	2.16248	3	2.4	6.55	597.1	4.7	0.258942
22	6.85	639	54.7	0.181667	7	2.00578	2.8	2.2	6.85	639.3	7.3	0.155959
23	7.35	732	62.6	0.475244	6	4.57819	6.3	5.1	7.35	731.3	5.8	0.448867
24	7.592	778	66.6	0.0403301	13	0.908685	1.2	1	7.592	780.7	12.6	0.0407924
25	7.803	818	70	0.0264744	18	0.74818	1	0.8	7.803	824.4	17.9	0.0236431
26	8.15	884	75.6	0.240214	5	1.48047	2	1.7	8.15	885.1	4.5	0.184194
27	8.45	950	81.3	0.152743	7	1.83102	2.5	2	8.45	949.8	6.7	0.153419
28	8.65	991	84.8	0.133083	6	1.35733	1.9	1.5	8.65	990.5	6	0.128414
29	9.3	1107	94.7	0.558315	9	7.70179	10.6	8.6	9.3	1104	8.5	0.509474

PeakCounter: 29

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.549	74	6.4	0.678178	4	5.11004	11.7	9	3.549	74.6	4.1	0.697296
2	4.124	151	13	0.427435	4	3.37671	7.7	6	4.124	151.4	4.4	0.428952
3	4.341	180	15.5	0.747594	4	5.81591	13.3	10.3	4.341	179.8	4.2	0.788224
4	4.818	240	20.6	0.399801	5	3.25818	7.5	5.8	4.818	239.8	4.6	0.402522
5	5.062	269	23.1	0.074564	5	0.54424	1.2	1	5.062	267.8	4.6	0.067439
6	5.154	280	24	0.0999506	2	0.415803	1	0.7	5.154	280.2	2.4	0.0992874
7	5.255	298	25.6	0.197061	5	1.80238	4.1	3.2	5.255	298.2	5.3	0.193367
8	5.303	309	26.5	0.712598	4	4.71604	10.8	8.3	5.303	308.8	3.9	0.682638
9	5.421	336	28.8	0.000333779	2	0.0362113	0.1	0.1	5.421	339.2	2.2	0.00932609
10	5.522	359	30.8	0.0947014	7	1.11992	2.6	2	5.522	359.7	6.6	0.0956977
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.561	368	31.6	0.158545	3	0.531515	1.2	0.9	5.561	368.5	2.6	0.113552
12	5.601	377	32.4	0.668515	3	3.5713	8.2	6.3	5.601	377.4	3.1	0.642812
13	5.666	392	33.6	0.436751	9	6.6157	15.1	11.7	5.666	392.8	8.7	0.429314
14	5.793	421	36.1	0.025201	10	0.728006	1.7	1.3	5.793	413.1	10.4	0.039541
15	5.871	439	37.7	0.0195194	6	0.222158	0.5	0.4	5.871	440.8	6.4	0.0196981
16	6.099	492	42.2	0.074755	2	0.330522	0.8	0.6	6.099	491.7	2.4	0.0774384
17	6.146	503	43.2	0.0255908	9	0.325349	0.7	0.6	6.146	506.9	9.2	0.0200163
18	6.219	520	44.6	0.405745	3	2.21528	5.1	3.9	6.219	520.4	3.1	0.403866
19	6.241	525	45.1	0.131878	5	1.02376	2.3	1.8	6.241	527.5	4.5	0.127516
20	6.383	558	47.9	0.0302026	14	0.702412	1.6	1.2	6.383	563.8	13.6	0.0291188
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.583	775	66.5	0.0169188	8	0.224218	0.5	0.4	7.583	774	8.3	0.0152903
22	7.758	809	69.4	0.0371338	16	1.02688	2.3	1.8	7.758	810.6	16	0.0362416

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	64	5.5	0.323136	10	5.51048	14.5	9.4	3.5	66	10.1	0.308652
2	4.55	209	17.9	0.409898	5	3.198	8.4	5.5	4.55	209.2	4.9	0.369549
3	5.2	287	24.6	0.56733	5	4.49675	11.8	7.7	5.2	286.9	4.7	0.54386
4	5.85	435	37.3	0.647795	4	4.38283	11.5	7.5	5.85	435.3	3.8	0.658392
5	6.55	601	51.5	0.499893	5	4.04513	10.6	6.9	6.55	599.9	4.8	0.476785
6	6.85	640	54.9	0.259538	7	2.77628	7.3	4.7	6.85	640.6	6.8	0.230331
7	7.35	732	62.8	0.376289	7	4.18541	11	7.1	7.35	730.6	6.8	0.346101
8	8.15	892	76.5	0.103254	9	1.6626	4.4	2.8	8.15	892.4	9.1	0.102686
9	8.45	949	81.4	0.151095	7	1.72377	4.5	2.9	8.45	948.6	6.5	0.149118
10	8.65	987	84.6	0.0939144	6	0.947881	2.5	1.6	8.65	986.6	5.8	0.0922351
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1104	94.7	0.434939	7	5.09467	13.4	8.7	9.3	1102	7.2	0.400889

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 134

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 10.900 4.550 8.800 5.200 11.300 5.850 13.300 6.550 11.800 6.850 7.000 7.350 10.100 8.150 3.700 8.450 4.300 8.650 2.600 9.300 16.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 9 PeakCount: 11 Return to Lane Navigator
3.500 10.200 4.082 5.600 4.297 8.800 4.443 0.100 4.550 2.300 4.767 4.300 4.942 0.500 5.008 0.700 5.100 0.500 5.200 4.900 5.285 4.800 5.427 0.100 5.503 0.300 5.592 5.200 5.667 8.000 5.850 4.600 6.115 0.500 6.174 0.200 6.247 2.500 6.285 2.400 6.460 3.800 6.550 2.900 6.850 2.600 7.350 5.900 7.632 1.400 7.793 0.500 8.150 2.800 8.450 2.000 8.650 1.600 9.300 10.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 8 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 10 PeakCount: 30 Return to Lane Navigator
3.531 11.700 4.125 7.900 4.342 14.300 4.803 7.400 5.023 0.800 5.175 2.800 5.252 2.400 5.300 12.800 5.444 0.100 5.518 0.300 5.562 0.500 5.610 8.600 5.680 12.800 5.875 0.900 6.107 1.700 6.170 1.400 6.242 8.500 6.440 1.500 7.577 2.200 7.741 1.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 9 LANE TYPE: Specimen ACTUAL GEL LANE: 11 PeakCount: 20 Return to Lane Navigator
3.500 8.600 4.550 8.200 5.200 10.400 5.850 14.300 6.550 11.700 6.850 7.100 7.350 11.800 8.150 4.200 8.450 3.400 8.650 2.400 9.300 17.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 10 LANE TYPE: pI Markers ACTUAL GEL LANE: 12 PeakCount: 11 Return to Lane Navigator
3.500 9.700 4.071 4.700 4.295 7.700 4.434 0.200 4.550 2.300 4.769 3.800 5.116 4.600 5.200 5.000 5.283 5.400 5.418 0.100 5.492 0 5.545 0.400 5.584 4.600 5.649 8.100 5.754 0 5.850 4.900 6.113 0.200 6.169 0.200 6.235 2.800 6.458 4.000 6.550 3.000 6.850 2.800 7.350 6.300 7.592 1.200 7.803 1.000 8.150 2.000 8.450 2.500 8.650 1.900 9.300 10.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 11 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 13 PeakCount: 29 Return to Lane Navigator
3.549 11.700 4.124 7.700 4.341 13.300 4.818 7.500 5.062 1.200 5.154 1.000 5.255 4.100 5.303 10.800 5.421 0.100 5.522 2.600 5.561 1.200 5.601 8.200 5.666 15.100 5.793 1.700 5.871 0.500 6.099 0.800 6.146 0.700 6.219 5.100 6.241 2.300 6.383 1.600 7.583 0.500 7.758 2.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 22 Return to Lane Navigator
3.500 14.500 4.550 8.400 5.200 11.800 5.850 11.500 6.550 10.600 6.850 7.300 7.350 11.000 8.150 4.400 8.450 4.500 8.650 2.500 9.300 13.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Cusk ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Cusk ---- Go to Display Gel Lane Navigator.