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Actinobacillus actinomycetemcomitans Search Results

Record: 1 of 1  
MiniMap tRNA-Gly-3 IGR1043 IGR1040 IGR1044 IGR1037 IGR1042 IGR1039 IGR1041 IGR1038 IGR1036 AA01605 orn, - AA01597 ybiP, - AA01596 prlC,opdA, - AA01603 ptsH, - AA01599 ybaN, - AA01604 crr, - AA01601 yjeQ, - AA01598 oppA, - AA01607 ptsI, - AA01600 AA01605 orn, - AA01597 ybiP, - AA01596 prlC,opdA, - AA01603 ptsH, - AA01599 ybaN, - AA01604 crr, - AA01601 yjeQ, - AA01598 oppA, - AA01607 ptsI, - AA01600 AA01605 orn, - AA01597 ybiP, - AA01596 prlC,opdA, - AA01603 ptsH, - AA01599 ybaN, - AA01604 crr, - AA01601 yjeQ, - AA01598 oppA, - AA01607 ptsI, - AA01600


Gene ID:AA01600

DNA Molecule Name:
1  

GenBank ID:


Gene Name:
ptsI  

Definition:
phosphotransferase system enzyme I

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
1080621

Gene Stop:
1082345

Gene Length:
1725

Molecular Weight*:
64847

pI*:


Net Charge*:


EC:
2.7.3.9  

Functional Class:
Transport and binding proteins; Carbohydrates, organic alcohols, and acids  

Gene Ontology:
Biological process
  GO:0009401    phosphoenolpyruvate-dependent sugar phosphotransferase system
  GO:0016310    phosphorylation

Cellular component
  GO:0005737    cytoplasm

Molecular function
  GO:0005351    sugar porter activity
  GO:0008965    phosphoenolpyruvate-protein phosphotransferase activity
  GO:0016772    transferase activity, transferring phosphorus-containing groups


Pathway: pathway table

Secondary Evidence:
De Reuse,H. and Danchin,A.
The ptsH, ptsI, and crr genes of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system: a complex
operon with several modes of transcription
J. Bacteriol. 170 (9), 3827-3837 (1988)
PubMed: 2457575

Saffen,D.W., Presper,K.A., Doering,T.L. and Roseman,S.
Sugar transport by the bacterial phosphotransferase system.
Molecular cloning and structural analysis of the Escherichia coli
ptsH, ptsI, and crr genes
J. Biol. Chem. 262 (33), 16241-16253 (1987)
PubMed: 2960675

Comment:
[FUNCTION] This is a component of the phosphoenolpyruvate dependent sugar phosphotransferase system (PTS), a major carbohydrate active transport system. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (pep) to
the phosphoryl carrier protein (hpr). Enzyme i is common to all pts (by similarity).
[CATALYTIC ACTIVITY] phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine.
[subunit] homodimer (by similarity).
[SUBCELLULAR LOCATION] Cytoplasmic.
[SIMILARITY] Belongs to the pep-utilizing enzymes family.

View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|15602762|ref|NP_245834.1|  PtsI [Pasteurella multocida su...   954   0.0  
 gi|53728564|ref|ZP_00132377.2|  COG1080: Phosphoenolpyruvate...   940   0.0  
 gi|152978669|ref|YP_001344298.1|  phosphoenolpyruvate-protei...   940   0.0  
 gi|113461240|ref|YP_719309.1|  phosphotransferase system, en...   937   0.0  
 gi|52425564|ref|YP_088701.1|  PtsA protein [Mannheimia succi...   925   0.0  
 gi|145633647|ref|ZP_01789374.1|  phosphoenolpyruvate-protein...   905   0.0  
 gi|53732545|ref|ZP_00154613.2|  COG1080: Phosphoenolpyruvate...   904   0.0  
 gi|145635431|ref|ZP_01791132.1|  phosphoenolpyruvate-protein...   904   0.0  
 gi|145631580|ref|ZP_01787346.1|  phosphoenolpyruvate-protein...   902   0.0  
 gi|16273599|ref|NP_439854.1|  phosphoenolpyruvate-protein ph...   901   0.0


InterPro Summary:  InterProScan

InterPro
IPR000121
Domain
PEP-utilizing enzyme
PD000940 [268-531]T 5e-137 PD000940 Q9CMD3_PASMU_Q9CMD3; Q9CMD3_PASMU_Q9CMD3;
PF02896 [250-542]T 1.3e-189 PF02896 PEP-utilizers_C PEP-utilizers_C
PS00742 [447-465]T 8e-5 PS00742 PEP_ENZYMES_2 PEP_ENZYMES_2
InterPro
IPR006318
Domain
Phosphoenolpyruvate-protein phosphotransferase
PR01736 [293-312]T 8.9e-31 PR01736 PHPHTRNFRASE[447-462]T 8.9e-31 PR01736 PHPHTRNFRASE[464-479]T 8.9e-31 PR01736 PHPHTRNFRASE[500-512]T 8.9e-31 PR01736 PHPHTRNFRASE PHPHTRNFRASE
TIGR01417 [2-566]T 0 TIGR01417 PTS_I_fam: phosphoenolpyruvate-protein phos PTS_I_fam: phosphoenolpyruvate-protein phos
InterPro
IPR008279
Domain
PEP-utilising enzyme, mobile region
PF00391 [145-225]T 4.2e-38 PF00391 PEP-utilizers PEP-utilizers
PS00370 [184-195]T 8e-5 PS00370 PEP_ENZYMES_PHOS_SITE PEP_ENZYMES_PHOS_SITE
InterPro
IPR008731
Domain
phosphotransferase system, PEP-utilising enzyme, N-terminal
PF05524 [2-127]T 1e-53 PF05524 PEP-utilisers_N PEP-utilisers_N
noIPR
unintegrated
unintegrated
G3DSA:1.10.274.10 [21-146]T 8.2e-34 G3DSA:1.10.274.10 no description no description
G3DSA:3.20.20.60 [250-573]T 1.7e-118 G3DSA:3.20.20.60 no description no description
G3DSA:3.50.30.10 [146-229]T 3.4e-15 G3DSA:3.50.30.10 no description no description
PTHR22931 [1-569]T 2.3e-247 PTHR22931 PHOSPHOENOLPYRUVATE DIKINASE-RELATED PHOSPHOENOLPYRUVATE DIKINASE-RELATED
PTHR22931:SF10 [1-569]T 2.3e-247 PTHR22931:SF10 PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE


COGS Summary:  COGS Search
No hits to the COGs database.
Blocks Summary:  Blocks Search

Significant hit ( 9.5e-81) to 7/7 blocks of the IPB000121 family, which is described as "PEP-utilizing enzyme". Interpro entry for IPR000121.
    IPB000121A    76-86    0.48
    IPB000121B    178-204    1.4e-18
    IPB000121C    288-300    3e-08
    IPB000121D    350-361    1.2e-07
    IPB000121E    388-398    0.00052
    IPB000121F    431-467    5.3e-25
    IPB000121G    496-509    3e-07
Significant hit ( 3.8e-44) to 6/12 blocks of the IPB002192 family, which is described as "Pyruvate phosphate dikinase, PEP/pyruvate binding domain". Interpro entry for IPR002192.
    IPB002192E    178-204    9.3e-08
    IPB002192G    270-301    1.4e-09
    IPB002192I    350-359    0.11
    IPB002192J    373-398    1.4
    IPB002192K    433-467    1.5e-16
    IPB002192L    496-513    9.8
Significant hit ( 2.2e-12) to 1/2 blocks of the IPB002114 family, which is described as "Serine phosphorylation site in HPr protein". Interpro entry for IPR002114.
    IPB002114B    496-539    2.2e-12

 ProDom Summary:  Protein Domain Search


Paralogs:  Local Blast Search

AA01600 has no significant similarity (blastp p-value < 1e-3) to any other gene in this genome.


Pfam Summary:  Pfam Search

Residues 250 to 542 (E-value = 1.2e-189) place AA01600 in the PEP-utilizers_C family which is described as PEP-utilising enzyme, TIM barrel domain (PF02896)


 Structural Feature(s):
Feature Type  Start  Stop
Uncleavable N-term signal seq  
1  
14

Top PDB Hits:


Gene Protein Sequence:
MISGIPASPGIVFGKALVLKEEKIVLDTQKIKDSQIEDEVARFYAGRDAA
VEQLNSIKDGAYQSLGEEKAAIFEGHLMILEDEELEEEIIDYLRSNHVNA
AVAANVIIDQQVAMLSEIDDEYLKERAGDIRDIGNRLIKNILGMHIVDLG
EINEEAILVAYDLTPSETAQLNLDKVLGFVTDIGGRTSHTSIMARSLELP
AIVGTNNVTHKVNTGDYLILDALNNVVYVNPSQEDIQRLKALQAKLVEEK
AELAKLKDLPALTLDGHRVDVVANIGTIRDVEGAERNGAEGVGLYRTEFL
FMDRDQLPTEEEQFIAYKEVVEAMNGNLVVLRTMDIGGDKELPYLNLPKE
MNPFLGWRAIRIALDRREILHAQLRAVLRASAYGKLAVMFPMIISVEEIR
ELKSVVEKLKVELRNEGKAFDEDIQIGVMVETPAAAVNAGFLAKEVDFFS
IGTNDLAQYTLAVDRGNELISHLYNPMSPSVLNLIKQVIDASHAEGKWTG
MCGELAGDERATILLLGMGLDEFSMSAISVPRIKKLIRNVNFQDAKALAE
KALQQPTAAEIESLVSDFLYEKALN

Gene Nucleotide Sequence:  Sequence Viewer
ATGATTTCAGGAATTCCGGCCTCTCCGGGTATTGTTTTTGGCAAAGCACT
TGTGCTTAAAGAAGAAAAAATTGTACTTGATACGCAAAAAATTAAAGACA
GCCAAATTGAAGATGAAGTGGCACGCTTTTATGCGGGACGCGATGCTGCG
GTTGAACAGTTAAATTCTATTAAAGATGGGGCCTATCAATCTTTGGGGGA
AGAGAAGGCGGCTATCTTTGAAGGTCATTTAATGATTTTGGAAGATGAGG
AACTGGAAGAAGAAATCATTGATTACCTTCGTTCAAATCATGTCAATGCT
GCAGTTGCCGCGAATGTGATTATCGATCAGCAAGTGGCGATGTTATCGGA
AATTGATGATGAATATTTGAAAGAACGTGCCGGCGATATTCGCGATATTG
GTAATCGTTTAATTAAAAATATTTTAGGTATGCATATCGTTGATTTAGGT
GAAATTAACGAAGAAGCGATTTTGGTTGCCTATGATTTAACCCCTTCCGA
AACCGCACAGTTAAATTTGGATAAAGTGTTAGGCTTTGTTACCGACATTG
GTGGTCGCACCTCCCATACTTCAATTATGGCGCGCTCTTTGGAATTGCCG
GCAATCGTGGGAACCAATAACGTTACCCATAAAGTGAATACCGGGGATTA
CCTGATCCTGGATGCGCTAAATAACGTAGTTTATGTCAATCCTTCTCAAG
AAGATATTCAACGCTTGAAAGCATTACAAGCGAAATTGGTGGAAGAAAAA
GCCGAATTAGCAAAATTAAAAGACTTACCGGCGCTCACGTTAGATGGTCA
CCGTGTCGATGTGGTGGCGAATATCGGTACAATCCGTGATGTGGAAGGCG
CTGAACGTAACGGCGCGGAAGGTGTTGGTTTATACCGTACCGAATTTTTG
TTCATGGACCGCGACCAGCTACCAACCGAAGAAGAACAGTTTATTGCGTA
TAAAGAAGTGGTAGAAGCCATGAATGGGAATCTCGTGGTGTTGCGTACCA
TGGATATCGGTGGCGATAAAGAACTGCCATATTTGAATCTGCCGAAAGAA
ATGAACCCGTTCCTTGGCTGGCGTGCAATTCGCATTGCGTTGGATCGTCG
TGAGATTCTACATGCGCAATTAAGAGCCGTGTTGCGCGCTTCTGCTTACG
GAAAACTAGCGGTGATGTTCCCGATGATTATTTCCGTGGAAGAAATTCGC
GAATTAAAATCGGTGGTTGAAAAATTAAAAGTTGAATTGCGTAACGAAGG
CAAAGCCTTTGATGAAGATATTCAAATCGGTGTAATGGTTGAAACCCCGG
CGGCGGCCGTCAATGCCGGATTCTTAGCAAAAGAAGTGGATTTCTTTAGT
ATCGGTACCAATGATTTAGCACAATACACTTTGGCGGTTGACCGTGGTAA
CGAGTTAATTTCCCATCTCTATAACCCAATGAGTCCATCGGTGCTGAACT
TAATTAAACAGGTGATTGATGCCTCTCATGCAGAAGGAAAATGGACCGGT
ATGTGTGGTGAGTTGGCAGGTGATGAACGCGCGACGATTCTATTGTTGGG
TATGGGCTTGGATGAGTTCAGTATGAGTGCCATTTCTGTGCCGCGCATTA
AGAAATTAATCCGTAATGTGAATTTCCAAGATGCAAAAGCCTTAGCTGAA
AAAGCGTTACAGCAACCGACGGCTGCCGAAATTGAAAGTTTGGTCTCTGA
TTTTTTGTATGAAAAAGCATTAAAT

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