| Mediators Inflamm. 1995 July; 4(4): 273–281. doi: 10.1155/S0962935195000445. | PMCID: PMC2365649 |
Copyright © 1995 Hindawi Publishing Corporation. Effect of dexamethasone on carrageenin-induced inflammation in the lung S. F. Smith,  1 A. Benjamin, 1 A. Dewar, 3 M. Sheppard, 4 B. Fox, 2 T. Smith, 1 A. Guz, 1 and T. D. Tetley 11
Department of Medicine,
Charing Cross and Westminster Medical School,
Fulham Palace Road,
London,
W6 8RF,
UK,
2
Department of Histopathology,
Charing Cross and Westminster Medical School,
Fulham Palace Road,
London,
W6 8RF,
UK,
3
Electron Microscopy Unit,
Royal Brompton Hospital,
Sydney Street,
London,
SW3 6NP,
UK,
4
Department of Histopathology,
Royal Brompton Hospital,
Sydney Street,
London,
SW3 6NP,
UK,
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract To study the anti-inflammatory mechanisms of glucocorticoids, we have compared the effects of intratracheal carrageenin (2.5 mg) on control rats and those in which inflammation was subdued by prior dexamethasone treatment (10 mg/l in drinking water). Inflammation was maximal 48 h post-carrageenin. After dexamethasone, carrageenin caused tittle inflammation or oedema (wet lung (mg), n = 6, mean ± S.E.M.; control, 995 ± 51; carrageenin + dexamethasone, 1144 ± 83; compared with carrageenin alone, 1881 ± 198), but rats had more lung lavage neutrophils than those given carrageenin alone (PMN × 10 6 /lung, mean ± S.E.M.; control, 0.055 ± 0.003; carrageenin + dexamethasone, 8.54 ± 1.52; compared with carrageenin alone, 6.30 ± 1.71). Proteolysis and partial inactivation of the anti-inflammatory mediator, lipocortin 1 (Lcl), in carrageenin-instilled rats was offset in those also given dexamethasone, by increased Lc1 levels (intact Lc1 ng/ml lavage fluid, n = 4, mean ± S.E.M.; control 24 ± 6; carrageenin 15 ± 4; carrageenin + dexamethasone, 40 ± 15). Maintenance of sufficient intact (fully active) extracellular Lc1 may contribute to the actions of glucocorticoids. Full Text The Full Text of this article is available as a PDF (2.2M). These references are in PubMed. This may not be the complete list of references from this article. - Witko-Sarsat, V; Descamps-Latscha, B. Neutrophil-derived Oxidants and Proteinases as Immunomodulatory Mediators in Inflammation. Mediators Inflamm. 1994;3(4):257–273. [PubMed]
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